FastQCFastQC Report
Fri 27 May 2016
SRR2140043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13842537
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142900.045.2791331
CTTATAC123900.039.6754381
GTATCAA270000.035.3534241
ATCAACG340700.031.7152143
TCAACGC342800.031.5458414
TATACAC178600.030.8958633
CAACGCA353100.030.5530725
TATCAAC362100.030.1002312
AACGCAG365200.029.6322946
TTATACA197300.026.7336062
ACGCAGA414700.026.0642977
CGCAGAG421800.025.5749448
ACACATC217400.023.337076
ACATCTC224650.023.2111768
TACACAT250550.022.0065085
GCAGAGT499250.021.5988799
CACATCT247350.020.4939987
CATCTCC258600.019.4868379
GAGTACT318850.019.08599512-13
AGAGTAC454850.019.0830910-11
Produced by FastQC (version 0.11.5)