##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13842537
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.694424295199646	18.0	18.0	30.0	18.0	33.0
2	31.070143717152426	32.0	31.0	33.0	27.0	33.0
3	31.844421438064423	33.0	31.0	33.0	29.0	33.0
4	32.62748642102239	33.0	33.0	33.0	31.0	34.0
5	33.01034044554116	33.0	33.0	34.0	32.0	34.0
6	36.993289886095305	38.0	37.0	38.0	35.0	38.0
7	37.21130895297589	38.0	38.0	38.0	36.0	38.0
8	37.13671771294525	38.0	38.0	38.0	36.0	38.0
9	37.37618357097402	38.0	38.0	38.0	37.0	38.0
10-11	37.44071877864585	38.0	38.0	38.0	37.0	38.0
12-13	37.47801678261723	38.0	38.0	38.0	37.0	38.0
14-15	37.447809711471244	38.0	38.0	38.0	37.0	38.0
16-17	37.45177903443567	38.0	38.0	38.0	37.0	38.0
18-19	37.41255717792194	38.0	38.0	38.0	37.0	38.0
20-21	37.49873506857495	38.0	38.0	38.0	37.0	38.0
22-23	37.52224755372136	38.0	38.0	38.0	37.0	38.0
24-25	37.53154181524073	38.0	38.0	38.0	38.0	38.0
26-27	37.5140051025035	38.0	38.0	38.0	37.5	38.0
28-29	37.45072969741236	38.0	38.0	38.0	37.0	38.0
30-31	37.48805145323732	38.0	38.0	38.0	37.0	38.0
32-33	37.47698557589331	38.0	38.0	38.0	37.0	38.0
34-35	37.45720313713676	38.0	38.0	38.0	37.0	38.0
36-37	37.43821064816295	38.0	38.0	38.0	37.0	38.0
38-39	37.41556213973175	38.0	38.0	38.0	37.0	38.0
40-41	37.40947450871558	38.0	38.0	38.0	37.0	38.0
42-43	37.34686308535973	38.0	38.0	38.0	37.0	38.0
44-45	37.356868079651754	38.0	38.0	38.0	37.0	38.0
46-47	37.33886189742918	38.0	38.0	38.0	37.0	38.0
48-49	37.30632191112453	38.0	38.0	38.0	37.0	38.0
50-51	37.27471687320632	38.0	38.0	38.0	37.0	38.0
52-53	37.25255286772918	38.0	38.0	38.0	37.0	38.0
54-55	37.23867683566738	38.0	38.0	38.0	37.0	38.0
56-57	37.23943747248062	38.0	38.0	38.0	37.0	38.0
58-59	37.23348636195534	38.0	38.0	38.0	37.0	38.0
60-61	37.229214855591835	38.0	38.0	38.0	37.0	38.0
62-63	37.22929762254836	38.0	38.0	38.0	37.0	38.0
64-65	37.22718244477227	38.0	38.0	38.0	37.0	38.0
66-67	37.226032064419094	38.0	38.0	38.0	37.0	38.0
68-69	37.22587656742152	38.0	38.0	38.0	37.0	38.0
70-71	37.230633298433375	38.0	38.0	38.0	37.0	38.0
72-73	37.200698109927615	38.0	38.0	38.0	37.0	38.0
74-75	37.19522576855107	38.0	38.0	38.0	37.0	38.0
76-77	37.19026529818239	38.0	38.0	38.0	37.0	38.0
78-79	37.17420843120813	38.0	38.0	38.0	36.0	38.0
80-81	37.166412117385654	38.0	38.0	38.0	36.0	38.0
82-83	37.16596140645588	38.0	38.0	38.0	36.0	38.0
84-85	37.14823896805686	38.0	38.0	38.0	36.0	38.0
86-87	37.13098018726667	38.0	38.0	38.0	36.0	38.0
88-89	37.11413514913848	38.0	38.0	38.0	36.0	38.0
90-91	37.10010144754381	38.0	38.0	38.0	36.0	38.0
92-93	37.09397188281602	38.0	38.0	38.0	36.0	38.0
94-95	37.05634424651332	38.0	38.0	38.0	36.0	38.0
96-97	37.06558434486384	38.0	38.0	38.0	36.0	38.0
98-99	37.05494404661565	38.0	38.0	38.0	36.0	38.0
100	35.43139264318818	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	18.0
19	42.0
20	111.0
21	393.0
22	1016.0
23	2564.0
24	5551.0
25	10382.0
26	17668.0
27	28446.0
28	44448.0
29	65029.0
30	90048.0
31	120332.0
32	158414.0
33	212436.0
34	316441.0
35	577118.0
36	1961858.0
37	1.0230218E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.40671236782679	18.967375705768386	19.721652179799122	23.9042597466057
2	13.93019342522276	21.16231546785564	42.36823575382696	22.53925535309464
3	17.607653857092814	26.10532303435418	31.231883288446333	25.055139820106675
4	12.006888621645007	18.478794746945592	39.37147504102752	30.14284159038188
5	12.936398805023492	37.62755650335789	36.12869918104186	13.307345510576768
6	29.125265757822472	38.46804296047893	19.117824899999928	13.28886638169867
7	25.673415212832733	32.90467636098787	23.474353003354803	17.94755542282459
8	23.584346882178284	37.028757670290545	21.88652178037069	17.50037366716048
9	24.8850082849805	17.108540721526193	21.730950847891197	36.275500145602116
10-11	23.5063594195197	27.085237337635437	29.53827033295992	19.870132909884944
12-13	24.442203044138513	24.540302836105838	29.45676070795404	21.560733411801607
14-15	21.639575291695106	26.04320841210021	27.036053942716855	25.28116235348783
16-17	21.321785161202747	29.20678846659395	27.744632360383072	21.726794011820232
18-19	21.277125717633986	28.22022437072048	29.50238095805704	21.000268953588495
20-21	21.983376724177415	27.36633132496263	29.395065797125614	21.25522615373435
22-23	21.6679371908335	27.545387766094866	29.224076249944375	21.562598793127265
24-25	21.55132543899686	27.56754169524196	29.424899255726906	21.456233610034275
26-27	21.512514105707876	27.704748260530852	29.3969816205643	21.385756013196975
28-29	21.289023863780173	27.614339676043397	29.55237966725761	21.544256792918816
30-31	21.675716652582935	27.37021805868335	29.27327046357965	21.68079482515407
32-33	21.633336319352097	27.502338081682975	29.442503773834883	21.421821825130046
34-35	21.651422860401823	27.78540935838329	29.332122242481745	21.23104553873314
36-37	21.495365065326205	27.59884035657708	29.29474666564959	21.611047912447127
38-39	21.366013661800956	27.56098646452748	29.5481885066637	21.524811367007864
40-41	21.63114901741643	27.407705396203593	29.466714429545483	21.4944311568345
42-43	21.732315623948566	27.408587739040343	29.33612342792872	21.522973209082373
44-45	21.584360253354888	27.68016766382766	29.186523766535878	21.548948316281578
46-47	21.821054930738068	27.471908829830987	28.828343713248973	21.878692526181972
48-49	21.70299850207639	27.52945000463935	28.9341232270149	21.833428266269358
50-51	21.551792108028184	27.682061099146527	28.97740538294556	21.78874140987973
52-53	21.70964434768649	27.693381444319648	29.048947243182187	21.54802696481167
54-55	21.68419563362788	27.642441316946815	28.844280004507798	21.829083044917503
56-57	21.869359483052076	27.895252392197058	28.53835871720591	21.697029407544964
58-59	21.545302316410716	28.15013941689942	28.869926852931876	21.43463141375798
60-61	21.80538717033178	28.39734755484209	28.426785622321983	21.370479652504144
62-63	21.56758387170539	28.617614347717723	28.386690057588655	21.428111722988234
64-65	21.554476052104445	28.564280677058466	28.381253120617	21.49999015022009
66-67	21.626118298769644	28.49345855585487	28.286797422261124	21.593625723114368
68-69	21.63909127675525	28.365830608496523	28.40059012948822	21.59448798526001
70-71	21.67555167643076	28.046072281490503	28.483645930791113	21.794730111287624
72-73	21.79996814113621	27.794085741555758	28.552923682503064	21.853022434804974
74-75	21.736278568157914	27.90662052814768	28.44417691272621	21.912923990968196
76-77	21.80006004727188	27.87165963276762	28.39727641619623	21.931003903764264
78-79	21.792587871556364	27.89490336807671	28.37409598471502	21.938412775651905
80-81	21.809021535579905	27.90673902149279	28.34710887093092	21.93713057199639
82-83	21.865394891596505	27.85782823994905	28.4147780120537	21.861998856400746
84-85	22.007185603081453	27.847552029765865	28.281371989778425	21.863890377374254
86-87	21.81256098051784	27.823555720987258	28.396135355773783	21.967747942721118
88-89	21.921456523288928	27.830508759710586	28.297055340113342	21.95097937688715
90-91	21.962379697210785	27.877506270832775	28.32353904688733	21.836574985069113
92-93	21.944466460421722	27.903790863135047	28.278044206338844	21.87369847010439
94-95	21.931566246923413	27.94408265889672	28.228931459665024	21.89541963451484
96-97	21.97208515304346	28.03668479605608	28.313093893686975	21.678136157213494
98-99	23.06302697530751	29.917254134491237	28.578563061929096	18.44115582827216
100	21.88294340191808	30.868259108617423	22.554374891302984	24.694422598161513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2566.0
1	2898.5
2	4084.0
3	5257.0
4	6445.5
5	7939.5
6	9261.5
7	13471.5
8	17753.5
9	19094.0
10	19589.5
11	20432.0
12	22670.5
13	26977.0
14	35363.5
15	43901.5
16	48239.5
17	48605.0
18	46035.5
19	42774.5
20	40617.5
21	41005.0
22	45889.5
23	55974.0
24	71997.5
25	94101.5
26	122025.0
27	153873.5
28	186448.0
29	221776.0
30	255799.5
31	288651.0
32	323679.5
33	357562.0
34	389648.5
35	419595.0
36	452139.5
37	481792.5
38	503687.5
39	522363.5
40	542695.0
41	559713.5
42	568859.0
43	577866.0
44	605627.5
45	638200.0
46	627444.0
47	605960.0
48	588888.5
49	561256.0
50	540922.5
51	515304.5
52	481631.0
53	448545.5
54	416009.5
55	379973.5
56	342149.0
57	304088.5
58	266131.0
59	229167.5
60	191153.0
61	153965.0
62	120069.5
63	91525.5
64	68691.0
65	50851.5
66	37748.5
67	28583.0
68	21314.5
69	15429.0
70	11287.0
71	8207.5
72	5689.0
73	3852.5
74	2611.0
75	1745.5
76	1116.5
77	681.5
78	435.0
79	278.0
80	173.0
81	111.0
82	79.0
83	57.0
84	37.0
85	17.5
86	9.5
87	8.5
88	5.0
89	2.5
90	3.5
91	3.5
92	2.5
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.1672327839903914E-4
3	0.0
4	0.0
5	0.006689525193250341
6	3.395331361584947E-4
7	0.0
8	2.8896437119871884E-5
9	1.3003396703942347E-4
10-11	0.0
12-13	0.0
14-15	7.224109279967971E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.39213353186377E-5
26-27	1.8063395219609162E-5
28-29	6.468595852502572E-4
30-31	2.4956768727794356E-4
32-33	0.001565461058721314
34-35	3.451182872949171E-4
36-37	0.0014183946682070408
38-39	7.588446923157884E-4
40-41	7.817023537758454E-4
42-43	1.4838275777516376E-4
44-45	6.725199816895226E-5
46-47	5.311671758274482E-4
48-49	0.0015989648734383221
50-51	0.0017950382463708748
52-53	0.0017657312583240928
54-55	0.0014955846069304537
56-57	0.003145067922500513
58-59	3.9891922118802966E-4
60-61	7.9659221038765E-6
62-63	0.001996608978593901
64-65	0.002525209023872953
66-67	0.003332670756899069
68-69	8.237570199931624E-4
70-71	0.005023074749631118
72-73	0.001182927582106198
74-75	0.00389980622971571
76-77	3.0689196801909056E-4
78-79	0.0010304923561482886
80-81	4.411771842572099E-6
82-83	3.5653900104367884E-5
84-85	9.678062187266361E-4
86-87	0.0
88-89	1.8385706473758933E-5
90-91	0.003081121323683181
92-93	4.557169075294957E-4
94-95	4.751756367947503E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	279.0
22-23	626.0
24-25	969.0
26-27	2956.0
28-29	7935.0
30-31	24727.0
32-33	31750.0
34-35	44513.0
36-37	69515.0
38-39	79770.0
40-41	79451.0
42-43	91433.0
44-45	107814.0
46-47	109883.0
48-49	102924.0
50-51	95353.0
52-53	95571.0
54-55	104366.0
56-57	106767.0
58-59	105468.0
60-61	107890.0
62-63	108903.0
64-65	117598.0
66-67	130475.0
68-69	135974.0
70-71	155947.0
72-73	116421.0
74-75	110958.0
76-77	115976.0
78-79	117657.0
80-81	115672.0
82-83	111602.0
84-85	111807.0
86-87	116201.0
88-89	118241.0
90-91	117627.0
92-93	116994.0
94-95	137097.0
96-97	415306.0
98-99	905649.0
100-101	9096386.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.60566377173882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.32437066907416	75.57347671449857
2	7.350354527884082	12.437632475784772
3	1.879173783637769	4.769662359213701
4	0.691306761231748	2.33953869615612
5	0.30521402154585187	1.2911417442664295
6	0.15938611223243498	0.8090980692853214
7	0.0916645982225627	0.5428740923892768
8	0.053117546025576855	0.3595236191535853
9	0.036950833983788414	0.2813624852505968
>10	0.10604486517102263	1.3580905701439985
>50	0.0018357809967657897	0.1059854682878295
>100	5.256234701624427E-4	0.07700057961218208
>500	2.7457388762197376E-5	0.017275717417281487
>1k	2.741913531528464E-5	0.03733740854042216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4448218559935942E-5
2	0.0	7.224109279967971E-6	0.0	0.0	1.4448218559935942E-5
3	0.0	1.4448218559935942E-5	0.0	0.0	1.4448218559935942E-5
4	0.0	2.1672327839903914E-5	0.0	0.0	1.4448218559935942E-5
5	0.0	2.1672327839903914E-5	0.0	0.0	1.4448218559935942E-5
6	0.0	2.1672327839903914E-5	0.0	0.0	2.8896437119871884E-5
7	0.0	2.8896437119871884E-5	0.0	0.0	2.8896437119871884E-5
8	0.0	4.334465567980783E-5	0.0	0.0	3.6120546399839856E-5
9	0.0	1.011375299195516E-4	0.0	1.4448218559935942E-5	4.334465567980783E-5
10-11	0.0	1.011375299195516E-4	0.0	3.6120546399839856E-5	4.334465567980783E-5
12-13	0.0	1.011375299195516E-4	0.0	5.05687649597758E-5	4.695671031979181E-5
14-15	0.0	1.4448218559935943E-4	0.0	7.585314743966369E-5	5.7792874239743774E-5
16-17	0.0	1.5531834951931138E-4	0.0	9.752547527956761E-5	7.224109279967971E-5
18-19	0.0	1.9143889591915125E-4	0.0	1.011375299195516E-4	7.585314743966369E-5
20-21	0.0	2.2394738767900712E-4	0.0	1.011375299195516E-4	9.030136599959965E-5
22-23	3.6120546399839855E-6	2.4923177015889503E-4	0.0	1.1558574847948753E-4	1.1919780311947152E-4
24-25	7.224109279967971E-6	2.60067934078847E-4	0.0	1.4087013095937545E-4	1.3725807631939145E-4
26-27	7.224109279967971E-6	2.60067934078847E-4	0.0	1.7337862271923132E-4	1.3725807631939145E-4
28-29	7.224109279967971E-6	2.6367998871883094E-4	0.0	3.792657371983185E-4	1.3725807631939145E-4
30-31	7.224109279967971E-6	3.178608083185907E-4	0.0	0.0012028141951146672	1.4448218559935943E-4
32-33	7.224109279967971E-6	3.3230902687852667E-4	0.0	0.003005229460466676	1.6615451343926334E-4
34-35	7.224109279967971E-6	4.009380650382224E-4	0.0	0.005197746626936955	1.7337862271923132E-4
36-37	7.224109279967971E-6	4.5873093927796614E-4	0.0	0.00834745827300299	1.8060273199919927E-4
38-39	7.224109279967971E-6	4.6956710319791815E-4	0.0	0.013028681086422236	1.8782684127916725E-4
40-41	7.224109279967971E-6	5.526443599175498E-4	0.0	0.017554585550322167	1.9505095055913523E-4
42-43	7.224109279967971E-6	5.670925784774857E-4	0.0	0.02242363520502058	1.986630051991192E-4
44-45	7.224109279967971E-6	6.032131248773256E-4	0.0	0.02801870784235578	2.0588711447908718E-4
46-47	7.224109279967971E-6	6.321095619971975E-4	0.0	0.034300070861287925	2.1311122375905516E-4
48-49	7.224109279967971E-6	6.465577805571333E-4	0.0	0.04031414183686126	2.3839560623894305E-4
50-51	7.224109279967971E-6	7.296350372767651E-4	0.0	0.04594533502059629	2.3839560623894305E-4
52-53	7.224109279967971E-6	7.44083255836701E-4	0.0	0.051731846553850644	2.7090409799879894E-4
54-55	7.224109279967971E-6	7.58531474396637E-4	0.0	0.0581468555944622	3.034125897586548E-4
56-57	7.224109279967971E-6	7.874279115165088E-4	0.0	0.06421510738963529	3.0702464439863876E-4
58-59	7.224109279967971E-6	8.127122939963968E-4	0.0	0.07051091862712738	3.1424875367860676E-4
60-61	1.4448218559935942E-5	8.416087311162686E-4	0.0	0.07662251507798029	3.178608083185907E-4
62-63	1.4448218559935942E-5	8.596690043161887E-4	0.0	0.0826401981081936	3.539813547184306E-4
64-65	1.4448218559935942E-5	9.752547527956761E-4	0.0	0.08862537264664708	3.539813547184306E-4
66-67	1.4448218559935942E-5	9.969270806355801E-4	0.0	0.09461415923974051	3.575934093584146E-4
68-69	1.4448218559935942E-5	0.001029435572395436	0.0	0.10044762748331465	3.6120546399839854E-4
70-71	1.4448218559935942E-5	0.0010438837909553717	0.0	0.10527333248233325	3.792657371983185E-4
72-73	1.4448218559935942E-5	0.0010511079002353397	0.0	0.1090515416357565	4.045501196782064E-4
74-75	1.4448218559935942E-5	0.0010872284466351798	0.0	0.11235657163134186	4.045501196782064E-4
76-77	1.4448218559935942E-5	0.0011414092662349394	0.0	0.11560380875268746	4.081621743181904E-4
78-79	1.4448218559935942E-5	0.0011811418672747632	0.0	0.11758321469539869	4.1177422895817436E-4
80-81	1.4448218559935942E-5	0.0011847539219147472	0.0	0.11829117740483555	4.262224475181103E-4
82-83	1.4448218559935942E-5	0.0012028141951146672	0.0	0.1187318480709136	4.551188846379822E-4
84-85	1.4448218559935942E-5	0.001242546796154491	0.0	0.11896663162251256	4.551188846379822E-4
86-87	1.4448218559935942E-5	0.0012858914518342989	0.0	0.11904609682459219	4.6234299391795014E-4
88	1.4448218559935942E-5	0.0013725807631939145	0.0	0.11906054504315214	4.6956710319791815E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14290	0.0	45.279133	1
CTTATAC	12390	0.0	39.675438	1
GTATCAA	27000	0.0	35.353424	1
ATCAACG	34070	0.0	31.715214	3
TCAACGC	34280	0.0	31.545841	4
TATACAC	17860	0.0	30.895863	3
CAACGCA	35310	0.0	30.553072	5
TATCAAC	36210	0.0	30.100231	2
AACGCAG	36520	0.0	29.632294	6
TTATACA	19730	0.0	26.733606	2
ACGCAGA	41470	0.0	26.064297	7
CGCAGAG	42180	0.0	25.574944	8
ACACATC	21740	0.0	23.33707	6
ACATCTC	22465	0.0	23.211176	8
TACACAT	25055	0.0	22.006508	5
GCAGAGT	49925	0.0	21.598879	9
CACATCT	24735	0.0	20.493998	7
CATCTCC	25860	0.0	19.486837	9
GAGTACT	31885	0.0	19.085995	12-13
AGAGTAC	45485	0.0	19.08309	10-11
>>END_MODULE