FastQCFastQC Report
Fri 27 May 2016
SRR2140042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5046313
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.047.0863081
CTTATAC48850.037.1383361
GTATCAA98500.035.4065741
ATCAACG121500.031.6904453
TCAACGC122450.031.4097214
TATACAC66950.031.1785813
CAACGCA124850.030.805935
TATCAAC129850.029.8498362
AACGCAG130250.029.5594776
TTATACA71900.026.8947412
ACGCAGA147050.026.095327
CGCAGAG149850.025.6077168
ACACATC81350.023.0867636
ACATCTC83050.022.716988
TACACAT94900.021.6359985
GCAGAGT178100.021.449999
GAGTACT111300.020.13409412-13
TACATGG124300.020.055792
CACATCT94650.019.617187
TGGTATC26650.019.5416242
Produced by FastQC (version 0.11.5)