##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140042_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5046313
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.594232264229348	18.0	18.0	30.0	18.0	33.0
2	31.044426891475023	32.0	31.0	33.0	27.0	33.0
3	31.822516161799715	33.0	31.0	33.0	29.0	33.0
4	32.62140200181796	33.0	33.0	33.0	31.0	34.0
5	33.01224914110559	33.0	33.0	34.0	32.0	34.0
6	36.99918098619725	38.0	37.0	38.0	35.0	38.0
7	37.23708002258282	38.0	38.0	38.0	36.0	38.0
8	37.16530940510428	38.0	38.0	38.0	36.0	38.0
9	37.39863421075942	38.0	38.0	38.0	37.0	38.0
10-11	37.465253146207935	38.0	38.0	38.0	37.0	38.0
12-13	37.49985474543493	38.0	38.0	38.0	37.0	38.0
14-15	37.47116300554484	38.0	38.0	38.0	37.0	38.0
16-17	37.47636026540566	38.0	38.0	38.0	37.0	38.0
18-19	37.43413438682856	38.0	38.0	38.0	37.0	38.0
20-21	37.51834001876589	38.0	38.0	38.0	37.0	38.0
22-23	37.54351514579875	38.0	38.0	38.0	38.0	38.0
24-25	37.55307075621269	38.0	38.0	38.0	38.0	38.0
26-27	37.53709369629267	38.0	38.0	38.0	38.0	38.0
28-29	37.47230480498357	38.0	38.0	38.0	37.0	38.0
30-31	37.510709013294715	38.0	38.0	38.0	37.5	38.0
32-33	37.499625433665415	38.0	38.0	38.0	37.5	38.0
34-35	37.477061435588915	38.0	38.0	38.0	37.0	38.0
36-37	37.45982115070956	38.0	38.0	38.0	37.0	38.0
38-39	37.43512448956572	38.0	38.0	38.0	37.0	38.0
40-41	37.431323089877324	38.0	38.0	38.0	37.0	38.0
42-43	37.372668674887706	38.0	38.0	38.0	37.0	38.0
44-45	37.383738775440136	38.0	38.0	38.0	37.0	38.0
46-47	37.3642440199267	38.0	38.0	38.0	37.0	38.0
48-49	37.331545829476994	38.0	38.0	38.0	37.0	38.0
50-51	37.300265457802794	38.0	38.0	38.0	37.0	38.0
52-53	37.278975562923236	38.0	38.0	38.0	37.0	38.0
54-55	37.265524869345214	38.0	38.0	38.0	37.0	38.0
56-57	37.268011356308946	38.0	38.0	38.0	37.0	38.0
58-59	37.26083058746955	38.0	38.0	38.0	37.0	38.0
60-61	37.25599731841248	38.0	38.0	38.0	37.0	38.0
62-63	37.25616040589089	38.0	38.0	38.0	37.0	38.0
64-65	37.256427915713275	38.0	38.0	38.0	37.0	38.0
66-67	37.25591167516266	38.0	38.0	38.0	37.0	38.0
68-69	37.25406957148407	38.0	38.0	38.0	37.0	38.0
70-71	37.259377734897896	38.0	38.0	38.0	37.0	38.0
72-73	37.22777882563848	38.0	38.0	38.0	37.0	38.0
74-75	37.22540173448604	38.0	38.0	38.0	37.0	38.0
76-77	37.22081802368278	38.0	38.0	38.0	37.0	38.0
78-79	37.20709491465723	38.0	38.0	38.0	37.0	38.0
80-81	37.20217207145211	38.0	38.0	38.0	36.5	38.0
82-83	37.203674996598934	38.0	38.0	38.0	36.5	38.0
84-85	37.185712180560685	38.0	38.0	38.0	36.0	38.0
86-87	37.168660767783976	38.0	38.0	38.0	36.0	38.0
88-89	37.15185562769005	38.0	38.0	38.0	36.0	38.0
90-91	37.14030861658725	38.0	38.0	38.0	36.0	38.0
92-93	37.136022175954544	38.0	38.0	38.0	36.0	38.0
94-95	37.09742950999466	38.0	38.0	38.0	36.0	38.0
96-97	37.1117937097427	38.0	38.0	38.0	36.0	38.0
98-99	37.10084033401694	38.0	38.0	38.0	36.0	38.0
100	35.51672812215866	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	6.0
19	8.0
20	40.0
21	146.0
22	328.0
23	795.0
24	1788.0
25	3325.0
26	5781.0
27	9525.0
28	15089.0
29	22289.0
30	31387.0
31	41920.0
32	54793.0
33	74608.0
34	111566.0
35	204541.0
36	712244.0
37	3756129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.33656632079699	18.93580521065578	19.757058272049317	23.970570196497917
2	14.020716944520187	21.152015415638854	42.25700631590962	22.570261323931337
3	17.647399200168522	25.939651385080552	31.234051474809434	25.178897939941496
4	12.09302712693406	18.451035439141407	39.26849166906611	30.187445764858424
5	13.061854450394383	37.4520485668722	36.057991383654866	13.428105599078554
6	29.22027286971758	38.321214531623326	19.08881196110173	13.369700637557367
7	25.74402340877389	32.81108405285205	23.432315831380258	18.012576706993798
8	23.62509492080553	37.016993796657836	21.850452369968405	17.50745891256823
9	24.968942622374193	17.087274748553643	21.717078139351468	36.226704489720696
10-11	23.624981248685923	26.95913828571474	29.488172850158122	19.927707615441214
12-13	24.509270431699342	24.452030621168365	29.401792952597273	21.63690599453502
14-15	21.728101289000502	25.925938402948844	26.98121380897301	25.364746499077643
16-17	21.425196970540668	29.07221569490438	27.723944194503986	21.778643140050963
18-19	21.425821188657938	28.12139278716956	29.403675515173155	21.04911050899934
20-21	22.101119500811194	27.32360376492167	29.25618085606648	21.31909587820066
22-23	21.806473629186474	27.459363072825184	29.122165878585687	21.611997419402655
24-25	21.75557734891952	27.559353638816315	29.221700443242938	21.46336856902123
26-27	21.756360709524014	27.755091847791295	29.131594756243572	21.356952686441115
28-29	21.56046671987965	27.7548919661483	29.160634606440038	21.524006707532017
30-31	22.150577771057183	27.421515144208854	29.104170840165967	21.323736244568
32-33	21.536229088660303	27.75746325788382	29.113058936521792	21.59324871693408
34-35	21.747139293810825	27.739678229123676	29.20626992624672	21.306912550818783
36-37	21.592825881929276	27.723340695442584	29.19633971688625	21.487493705741894
38-39	21.471297103169068	27.43029575426567	29.46029370705667	21.638113435508586
40-41	21.77547576408509	27.317438043994013	29.345395683467807	21.561690508453086
42-43	21.86321402575257	27.319801700155445	29.18977502162078	21.627209252471207
44-45	21.721676552097495	27.565601369582627	29.07245636549412	21.640265712825755
46-47	21.92112390102345	27.334661658548644	28.745148829947837	21.99906561048007
48-49	21.814048807819706	27.374877798832088	28.862282336834955	21.94879105651325
50-51	21.670252520248134	27.549790139733638	28.8910298121282	21.888927527890033
52-53	21.820033204534273	27.540445176435778	28.953720186892333	21.685801432137612
54-55	21.76438745644659	27.536990565113896	28.72160278049828	21.97701919794124
56-57	21.987790717335205	27.71836068561011	28.451092113054116	21.84275648400057
58-59	21.64243502008301	28.037337093355873	28.80627068187083	21.513957204690282
60-61	21.916406800230895	28.22021339169238	28.354226704302114	21.509153103774608
62-63	21.632400814739913	28.470590626779014	28.326308703878812	21.570699854602267
64-65	21.63500574065611	28.413193476831154	28.333515399449343	21.618285383063395
66-67	21.70560810546784	28.368788336295562	28.200378620625894	21.725224937610705
68-69	21.762336558589315	28.193734434693575	28.338445099900344	21.70548390681676
70-71	21.757932655186	27.899986146549242	28.385835762101586	21.956245436163172
72-73	21.915147295916874	27.737996515165282	28.416275953555605	21.930580235362243
74-75	21.816763755913566	27.80067378307578	28.37040279126921	22.012159669741436
76-77	21.90781634181615	27.77390094870271	28.293697465743605	22.024585243737533
78-79	21.883767674808887	27.796684259773656	28.278756470579324	22.040791594838133
80-81	21.921745075366804	27.78214696766814	28.242003359895552	22.0541045970695
82-83	21.978280603426793	27.778071060455872	28.29818401821114	21.9454643179062
84-85	22.10449078126849	27.72575021029181	28.19641689051902	21.973342117920684
86-87	21.932438108754408	27.721852878468294	28.256508202966206	22.089200809811093
88-89	22.028591004109597	27.75869249309977	28.189106289052628	22.02361021373801
90-91	22.06235540659186	27.820611249607474	28.19121897450029	21.925814369300376
92-93	22.0892902555472	27.765019039597544	28.184929722381437	21.960760982473822
94-95	22.00186665544103	27.851419787255832	28.127077349223377	22.019636208079756
96-97	22.09587078858729	27.924502097200993	28.210602191048217	21.7690249231635
98-99	23.14857670335494	29.81919877190532	28.463887996843358	18.568336527896385
100	21.953549730359097	30.836090276710664	22.41688020703951	24.793479785890725
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	904.0
1	1005.0
2	1497.0
3	2049.0
4	2448.5
5	2921.0
6	3427.0
7	5016.0
8	6581.0
9	6989.0
10	7196.5
11	7528.0
12	8209.0
13	9679.0
14	12583.5
15	15560.5
16	17039.0
17	17169.0
18	16370.0
19	15221.0
20	14489.0
21	14684.0
22	16533.0
23	20508.0
24	26456.0
25	34593.0
26	45052.5
27	56744.0
28	67984.0
29	79734.5
30	91076.0
31	102324.0
32	114937.0
33	127107.0
34	138913.5
35	150160.5
36	162349.5
37	172941.0
38	180251.5
39	187664.0
40	195711.0
41	201922.5
42	207365.0
43	221313.5
44	227461.0
45	223757.0
46	221390.5
47	215334.0
48	209700.0
49	204340.0
50	198585.5
51	189955.0
52	179088.5
53	168082.5
54	156718.0
55	144076.0
56	130759.5
57	117372.0
58	103321.5
59	88934.5
60	73756.5
61	59197.0
62	46472.5
63	35451.5
64	26436.5
65	19548.5
66	14417.5
67	10959.5
68	8290.0
69	6028.0
70	4382.0
71	3127.5
72	2193.0
73	1465.5
74	943.5
75	629.5
76	392.0
77	234.0
78	144.5
79	87.5
80	64.0
81	41.5
82	29.5
83	20.5
84	11.5
85	5.5
86	3.5
87	3.5
88	2.5
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.783480335048579E-4
3	0.0
4	0.0
5	0.006380896309840472
6	4.359618596785415E-4
7	0.0
8	1.9816448167206435E-5
9	1.5853158533765148E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.918161598277249E-5
26-27	1.9819766967107907E-5
28-29	6.740794255891652E-4
30-31	2.976632541558999E-4
32-33	0.0017299465883961944
34-35	2.990797316656648E-4
36-37	0.001421223531338129
38-39	7.246705013814032E-4
40-41	8.505569426609188E-4
42-43	1.222662788380302E-4
44-45	6.158929955721399E-5
46-47	5.693677264566963E-4
48-49	0.0017848410630346369
50-51	0.001914382100569434
52-53	0.0019921899794751448
54-55	0.001356228463198794
56-57	0.0031877006447555327
58-59	3.91003742448876E-4
60-61	1.0953273226881392E-5
62-63	0.0022757580179871054
64-65	0.0024634053335066265
66-67	0.003513339033500701
68-69	9.109094155240827E-4
70-71	0.005319994808974762
72-73	0.0011781621463734654
74-75	0.004217593419988777
76-77	3.688427356899132E-4
78-79	0.0011180660967014164
80-81	0.0
82-83	3.683083438168211E-5
84-85	0.001116251933596405
86-87	0.0
88-89	2.5347527272671965E-5
90-91	0.0033836115798466966
92-93	5.832778895295008E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	25.0
20-21	124.0
22-23	220.0
24-25	324.0
26-27	1074.0
28-29	3067.0
30-31	9562.0
32-33	12701.0
34-35	17360.0
36-37	26521.0
38-39	30008.0
40-41	29992.0
42-43	34545.0
44-45	40603.0
46-47	40346.0
48-49	37539.0
50-51	35132.0
52-53	35525.0
54-55	38871.0
56-57	39513.0
58-59	38736.0
60-61	38963.0
62-63	39628.0
64-65	43237.0
66-67	48080.0
68-69	49978.0
70-71	56977.0
72-73	43357.0
74-75	41334.0
76-77	43058.0
78-79	43666.0
80-81	43078.0
82-83	41349.0
84-85	42309.0
86-87	43449.0
88-89	43841.0
90-91	43813.0
92-93	43798.0
94-95	50772.0
96-97	152939.0
98-99	328001.0
100-101	3292898.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.07316782147781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.85795115210831	81.82078698708855
2	6.1163064696486025	10.89597585237201
3	1.2386259800400239	3.3098501936444245
4	0.4153111016979386	1.479723018386533
5	0.16106662426186655	0.7173357226658075
6	0.07616612421158148	0.40706147785258323
7	0.040074865364830126	0.24987166456452498
8	0.027414851541820925	0.1953542137748412
9	0.017656474454788514	0.1415446301022312
>10	0.04795130218903195	0.632077485790387
>50	0.0010756286176622565	0.06610426589702442
>100	3.6930529436639124E-4	0.06475589528585424
>500	3.0120569137147654E-5	0.019558592575214202
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9816448167206435E-5	0.0
2	0.0	0.0	0.0	3.963289633441287E-5	0.0
3	0.0	0.0	0.0	3.963289633441287E-5	0.0
4	0.0	5.94493445016193E-5	0.0	3.963289633441287E-5	0.0
5	0.0	5.94493445016193E-5	0.0	3.963289633441287E-5	0.0
6	0.0	5.94493445016193E-5	0.0	3.963289633441287E-5	0.0
7	0.0	5.94493445016193E-5	0.0	3.963289633441287E-5	0.0
8	0.0	5.94493445016193E-5	0.0	3.963289633441287E-5	0.0
9	0.0	9.908224083603217E-5	0.0	3.963289633441287E-5	0.0
10-11	0.0	9.908224083603217E-5	0.0	5.94493445016193E-5	0.0
12-13	0.0	1.089904649196354E-4	0.0	6.935756858522252E-5	0.0
14-15	0.0	1.8825625758846112E-4	0.0	7.926579266882574E-5	9.908224083603218E-6
16-17	0.0	1.9816448167206434E-4	0.0	7.926579266882574E-5	1.9816448167206435E-5
18-19	0.0	2.2788915392287399E-4	0.0	7.926579266882574E-5	2.9724672250809654E-5
20-21	9.908224083603218E-6	2.576138261736836E-4	0.0	9.908224083603217E-5	3.963289633441287E-5
22-23	1.9816448167206435E-5	2.873384984244933E-4	0.0	1.5853158533765148E-4	3.963289633441287E-5
24-25	1.9816448167206435E-5	3.566960670097158E-4	0.0	2.1798092983927076E-4	3.963289633441287E-5
26-27	1.9816448167206435E-5	3.963289633441287E-4	0.0	2.477056020900804E-4	3.963289633441287E-5
28-29	1.9816448167206435E-5	3.963289633441287E-4	0.0	6.242181172670027E-4	3.963289633441287E-5
30-31	1.9816448167206435E-5	4.1614541151133513E-4	0.0	0.0015456829570421019	3.963289633441287E-5
32-33	4.9541120418016086E-5	4.1614541151133513E-4	0.0	0.0037155840313512064	3.963289633441287E-5
34-35	5.94493445016193E-5	4.4587008376214475E-4	0.0	0.007322177597782777	3.963289633441287E-5
36-37	5.94493445016193E-5	4.5577830784574797E-4	0.0	0.012088033381995926	3.963289633441287E-5
38-39	5.94493445016193E-5	4.5577830784574797E-4	0.0	0.018637369501257654	3.963289633441287E-5
40-41	9.908224083603217E-5	5.251358764309705E-4	0.0	0.025573126359779902	5.94493445016193E-5
42-43	9.908224083603217E-5	5.548605486817801E-4	0.0	0.03277640526855944	5.94493445016193E-5
44-45	9.908224083603217E-5	6.044016690997963E-4	0.0	0.04104977237836813	5.94493445016193E-5
46-47	9.908224083603217E-5	6.341263413506059E-4	0.0	0.05035359479287155	5.94493445016193E-5
48-49	9.908224083603217E-5	6.539427895178123E-4	0.0	0.05969705010370938	5.94493445016193E-5
50-51	9.908224083603217E-5	7.133921340194316E-4	0.0	0.06860454355486867	7.926579266882574E-5
52-53	9.908224083603217E-5	7.133921340194316E-4	0.0	0.0773237807484395	9.908224083603217E-5
54-55	9.908224083603217E-5	7.133921340194316E-4	0.0	0.08528999291165648	9.908224083603217E-5
56-57	9.908224083603217E-5	7.133921340194316E-4	0.0	0.09142318361940688	1.188986890032386E-4
58-59	9.908224083603217E-5	7.530250303538445E-4	0.0	0.09737802629365241	1.188986890032386E-4
60-61	1.188986890032386E-4	7.728414785210509E-4	0.0	0.10351121700140281	1.188986890032386E-4
62-63	1.188986890032386E-4	7.926579266882574E-4	0.0	0.10929761986622709	1.188986890032386E-4
64-65	1.288069130868418E-4	8.322908230226703E-4	0.0	0.11497503226613173	1.188986890032386E-4
66-67	1.3871513717044504E-4	8.71923719357083E-4	0.0	0.12021648280635783	1.288069130868418E-4
68-69	1.3871513717044504E-4	8.71923719357083E-4	0.0	0.12561646493192158	1.3871513717044504E-4
70-71	1.3871513717044504E-4	8.818319434406863E-4	0.0	0.13046158650880355	1.6843980942125468E-4
72-73	1.3871513717044504E-4	9.016483916078927E-4	0.0	0.13424652810874	2.1798092983927076E-4
74-75	1.5853158533765148E-4	9.511895120259088E-4	0.0	0.13743697626366022	2.1798092983927076E-4
76-77	1.5853158533765148E-4	9.908224083603216E-4	0.0	0.14082558890025254	2.1798092983927076E-4
78-79	1.6843980942125468E-4	0.001010638856527528	0.0	0.14310448043948126	2.1798092983927076E-4
80-81	1.783480335048579E-4	0.001010638856527528	0.0	0.14366924921224666	2.2788915392287399E-4
82-83	1.783480335048579E-4	0.001010638856527528	0.0	0.14409530284784158	2.377973780064772E-4
84-85	1.783480335048579E-4	0.0010205470806111312	0.0	0.14427365088134644	2.377973780064772E-4
86-87	1.783480335048579E-4	0.0010304553046947345	0.0	0.14440245779443328	2.377973780064772E-4
88	1.783480335048579E-4	0.0010700882010291474	0.0	0.14440245779443328	2.377973780064772E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5240	0.0	47.086308	1
CTTATAC	4885	0.0	37.138336	1
GTATCAA	9850	0.0	35.406574	1
ATCAACG	12150	0.0	31.690445	3
TCAACGC	12245	0.0	31.409721	4
TATACAC	6695	0.0	31.178581	3
CAACGCA	12485	0.0	30.80593	5
TATCAAC	12985	0.0	29.849836	2
AACGCAG	13025	0.0	29.559477	6
TTATACA	7190	0.0	26.894741	2
ACGCAGA	14705	0.0	26.09532	7
CGCAGAG	14985	0.0	25.607716	8
ACACATC	8135	0.0	23.086763	6
ACATCTC	8305	0.0	22.71698	8
TACACAT	9490	0.0	21.635998	5
GCAGAGT	17810	0.0	21.44999	9
GAGTACT	11130	0.0	20.134094	12-13
TACATGG	12430	0.0	20.05579	2
CACATCT	9465	0.0	19.61718	7
TGGTATC	2665	0.0	19.541624	2
>>END_MODULE