FastQCFastQC Report
Fri 27 May 2016
SRR2140041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5313911
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89400.048.3825951
CTTATAC57600.034.6586881
GTATCAA167850.033.8895871
TATACAC74450.029.6144183
ATCAACG211450.028.626913
TCAACGC215550.028.1753834
TATCAAC218550.027.9353642
CAACGCA219550.027.5342435
AACGCAG224650.026.978766
TTATACA86250.024.586792
ACGCAGA252250.023.97927
CGCAGAG257150.023.5378658
TGGTATC39800.023.4681072
ACACATC89250.023.2198246
GTGGTAT38400.022.6534181
ACATCTC90800.022.6027228
TACACAT98550.022.0875955
GCAGAGT284700.021.1475039
CACATCT96950.021.0448887
GGACCGA10300.021.015186
Produced by FastQC (version 0.11.5)