##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5313911
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.194399191104253	18.0	18.0	30.0	18.0	33.0
2	31.310990342141597	32.0	32.0	33.0	27.0	33.0
3	31.974039836195978	33.0	31.0	33.0	29.0	33.0
4	32.67171260489685	33.0	33.0	33.0	31.0	34.0
5	33.05011487772377	33.0	33.0	34.0	33.0	34.0
6	37.083047495526365	38.0	38.0	38.0	36.0	38.0
7	37.335178364861584	38.0	38.0	38.0	37.0	38.0
8	37.29227851200368	38.0	38.0	38.0	36.0	38.0
9	37.48791483334968	38.0	38.0	38.0	37.0	38.0
10-11	37.53233766993839	38.0	38.0	38.0	37.5	38.0
12-13	37.56171292669373	38.0	38.0	38.0	38.0	38.0
14-15	37.531552749001634	38.0	38.0	38.0	38.0	38.0
16-17	37.53913831074702	38.0	38.0	38.0	38.0	38.0
18-19	37.49112678025658	38.0	38.0	38.0	38.0	38.0
20-21	37.573475473387816	38.0	38.0	38.0	38.0	38.0
22-23	37.59184558309081	38.0	38.0	38.0	38.0	38.0
24-25	37.59769796625109	38.0	38.0	38.0	38.0	38.0
26-27	37.57777687214893	38.0	38.0	38.0	38.0	38.0
28-29	37.50843820167309	38.0	38.0	38.0	38.0	38.0
30-31	37.54564317552547	38.0	38.0	38.0	38.0	38.0
32-33	37.53054749537457	38.0	38.0	38.0	38.0	38.0
34-35	37.50596870986354	38.0	38.0	38.0	38.0	38.0
36-37	37.48454693824398	38.0	38.0	38.0	38.0	38.0
38-39	37.454722018090344	38.0	38.0	38.0	38.0	38.0
40-41	37.45069345656826	38.0	38.0	38.0	38.0	38.0
42-43	37.38364866913028	38.0	38.0	38.0	37.0	38.0
44-45	37.38616613380299	38.0	38.0	38.0	37.0	38.0
46-47	37.3614146744421	38.0	38.0	38.0	37.0	38.0
48-49	37.32581870054847	38.0	38.0	38.0	37.0	38.0
50-51	37.29814633428328	38.0	38.0	38.0	37.0	38.0
52-53	37.281177934916336	38.0	38.0	38.0	37.0	38.0
54-55	37.272616919020145	38.0	38.0	38.0	37.0	38.0
56-57	37.27747370698125	38.0	38.0	38.0	37.0	38.0
58-59	37.27474148636205	38.0	38.0	38.0	37.0	38.0
60-61	37.27457616751789	38.0	38.0	38.0	37.0	38.0
62-63	37.27741186257718	38.0	38.0	38.0	37.0	38.0
64-65	37.2825097804221	38.0	38.0	38.0	37.0	38.0
66-67	37.28729040563736	38.0	38.0	38.0	37.0	38.0
68-69	37.293021328207395	38.0	38.0	38.0	37.0	38.0
70-71	37.30408257734757	38.0	38.0	38.0	37.0	38.0
72-73	37.276079828124566	38.0	38.0	38.0	37.0	38.0
74-75	37.277596453954104	38.0	38.0	38.0	37.0	38.0
76-77	37.28034669435617	38.0	38.0	38.0	37.0	38.0
78-79	37.266788094815624	38.0	38.0	38.0	37.0	38.0
80-81	37.262258753716964	38.0	38.0	38.0	37.0	38.0
82-83	37.26366511614688	38.0	38.0	38.0	37.0	38.0
84-85	37.247013781242174	38.0	38.0	38.0	37.0	38.0
86-87	37.23413039579087	38.0	38.0	38.0	37.0	38.0
88-89	37.21665727240092	38.0	38.0	38.0	37.0	38.0
90-91	37.2047062798633	38.0	38.0	38.0	36.5	38.0
92-93	37.19952818369606	38.0	38.0	38.0	36.0	38.0
94-95	37.16023951607189	38.0	38.0	38.0	36.0	38.0
96-97	37.17196729356003	38.0	38.0	38.0	36.0	38.0
98-99	37.15837252504341	38.0	38.0	38.0	36.0	38.0
100	35.60040939407728	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	4.0
19	14.0
20	48.0
21	116.0
22	317.0
23	851.0
24	1825.0
25	3514.0
26	6068.0
27	10159.0
28	15786.0
29	23139.0
30	32720.0
31	42139.0
32	54358.0
33	71962.0
34	107086.0
35	202091.0
36	783994.0
37	3957716.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.534480536087266	19.71596814474311	18.541691797246887	23.207859521922742
2	14.20836723030198	21.581204904799623	42.11169316981492	22.09873469508348
3	17.365928785785083	26.72925835603946	31.983994462835376	23.920818395340078
4	12.0925811516226	18.857146835918027	39.229731171636104	29.820540840823266
5	12.66932303907472	37.91729462547558	35.92181592889976	13.491566406549943
6	28.413637311409417	38.676585824476675	19.148187430230045	13.761589433883865
7	25.03282422306283	33.72933795842648	23.52386029799897	17.71397752051173
8	23.710492595940202	36.4149066158265	22.08460852453439	17.78999226369891
9	25.21607007733114	16.6763739106167	21.9618506253692	36.14570538668296
10-11	23.474612201822726	27.139737568054866	29.409271250497042	19.976378979625366
12-13	24.51290584279639	25.061466027564254	29.332999743503418	21.092628386135935
14-15	21.829844346282805	26.440102214734118	27.35870058794737	24.371352851035706
16-17	20.839490913566298	29.49852754402548	28.141843173511937	21.52013836889628
18-19	20.893838831700418	28.10167501864446	30.3652338174275	20.63925233222762
20-21	21.907037539132972	27.16046803038265	30.13452207395594	20.79797235652844
22-23	21.55087750751069	27.407889159086924	29.936750684592013	21.10448264881037
24-25	21.394668435428	27.50565469255182	30.154878388553435	20.944798483466748
26-27	21.23310905356425	27.683569344398627	30.14410743115896	20.939214170878152
28-29	20.800707107579765	27.563267334174057	30.28176139796519	21.354264160280987
30-31	21.474440217691676	27.272566663470226	30.332021848527518	20.920971270310577
32-33	21.053234554263174	27.71384165177381	29.994231902190055	21.23869189177296
34-35	21.146238389901036	27.749583166107232	30.326040845431606	20.77813759856012
36-37	21.404569740819223	27.56139875632943	29.924779987729806	21.109251515121542
38-39	21.075489918276002	27.453441253930123	30.1579728367636	21.313095991030277
40-41	21.312805938949815	27.27870857009339	30.191504159162353	21.216981331794443
42-43	21.545436640894067	27.309409105913616	29.88441363338975	21.260740619802565
44-45	21.330812439301216	27.66299794080817	29.64161269769386	21.364576922196754
46-47	21.61864470142812	27.452229130670315	29.18533078346202	21.74379538443955
48-49	21.522092998505705	27.517370600572384	29.197591188549367	21.76294521237255
50-51	21.354613646436434	27.83205595744161	29.02924919404059	21.784081202081364
52-53	21.576022969652136	27.913377296268838	28.918841795734163	21.591757938344863
54-55	21.534623216716536	28.0379357114071	28.61611895199604	21.81132211988032
56-57	21.82120506227854	28.19608103846291	28.220533748068753	21.762180151189796
58-59	21.362808681435336	28.486460578326962	28.721174143429423	21.42955659680828
60-61	21.708478875057164	28.753904726634065	28.169706559828523	21.36790983848025
62-63	21.448712478695374	28.885194745822062	28.145070918288123	21.52102185719444
64-65	21.365245227206927	28.86286652485878	28.180477190961266	21.591411056973026
66-67	21.530368794733967	28.821132178186748	28.04388673173141	21.604612295347874
68-69	21.558847938406736	28.632932759246504	28.12004546637831	21.68817383596845
70-71	21.558552720425855	28.245033611232827	28.285280621489623	21.911133046851695
72-73	21.755125180411795	27.96110532087925	28.42162267878407	21.862146819924888
74-75	21.65400204130443	28.07760073709163	28.28135413816597	21.987043083437975
76-77	21.697398168455006	28.032968984403155	28.220315971065713	22.049316876076126
78-79	21.758902017183747	28.054571124466765	28.157569295074968	22.02895756327452
80-81	21.71444521039259	28.05240668205774	28.19058073730995	22.04256737023972
82-83	21.857837477401567	28.021827507341516	28.22385410212406	21.896480913132855
84-85	22.020744903506518	28.013928919412205	28.04209842080522	21.923227756276056
86-87	21.786183683193823	28.017610741317352	28.169032217694745	22.02717335779408
88-89	21.88708177405036	27.964603912794843	28.091434823778382	22.056879489376417
90-91	21.9651089669591	28.017848267376134	28.06041578044046	21.9566269852243
92-93	22.016598400743565	27.992906058334636	28.019218249494383	21.971277291427416
94-95	21.85521753689784	28.077431120845326	28.033113804109743	22.034237538147092
96-97	21.92163108671659	28.227233680166535	28.060413583476063	21.79072164964081
98-99	22.986161318314355	30.081831229771467	28.294243684173733	18.637763767740452
100	29.101072467761256	41.21038894292742	29.68853858931132	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3027.0
1	2586.5
2	2360.5
3	2729.0
4	3515.5
5	4664.0
6	5310.0
7	5553.0
8	5487.5
9	5405.5
10	5750.5
11	7028.5
12	9220.0
13	14359.0
14	22385.0
15	29046.0
16	33359.5
17	34678.5
18	33700.5
19	31341.0
20	28750.5
21	27533.5
22	28622.0
23	33188.5
24	40591.5
25	50439.0
26	63214.5
27	77070.0
28	90724.0
29	105410.5
30	118631.0
31	130811.5
32	144178.0
33	157844.5
34	171033.0
35	183138.5
36	195434.5
37	205895.5
38	211360.5
39	214271.5
40	218919.0
41	223309.5
42	227568.0
43	242306.5
44	249466.5
45	245761.0
46	241220.5
47	232685.0
48	225812.5
49	217560.5
50	209104.0
51	200080.5
52	188531.0
53	175312.0
54	162538.0
55	149338.5
56	134671.0
57	119885.0
58	106121.0
59	91869.5
60	77141.0
61	63486.0
62	50598.0
63	39470.5
64	30790.5
65	23430.5
66	16934.0
67	12617.5
68	9668.5
69	7221.0
70	5326.0
71	3876.5
72	2840.5
73	2098.5
74	1521.5
75	1044.5
76	697.5
77	453.0
78	317.5
79	215.0
80	143.5
81	93.0
82	67.5
83	57.5
84	43.0
85	27.5
86	17.0
87	11.5
88	6.5
89	3.0
90	1.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.6345943693825507E-4
3	0.0
4	0.0
5	0.0075085939527402695
6	3.575520929876319E-4
7	0.0
8	3.763706241975072E-5
9	1.8818531209875364E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.695415896822263E-4
26-27	9.422726995613438E-6
28-29	6.789635131609061E-4
30-31	2.739797466613206E-4
32-33	0.0015173982992241163
34-35	2.3832641471751407E-4
36-37	0.0014883801680160352
38-39	7.854844795539844E-4
40-41	7.840757354434379E-4
42-43	6.934860164984286E-5
44-45	1.2044390806757385E-4
46-47	5.500650910357725E-4
48-49	0.0017461738901954197
50-51	0.0017719416706159646
52-53	0.0018924131244165946
54-55	0.001464278758192559
56-57	0.003318471891724375
58-59	4.649086919329044E-4
60-61	5.6112440352475906E-5
62-63	0.002219775788985154
64-65	0.0025175096840398315
66-67	0.0033436615322886246
68-69	7.997015084280184E-4
70-71	0.005355455940566826
72-73	0.001250179326465269
74-75	0.004122154001411335
76-77	2.936264423122342E-4
78-79	0.0013494161245106258
80-81	0.0
82-83	4.022562823045316E-5
84-85	9.262612920446006E-4
86-87	0.0
88-89	1.4078243215448223E-5
90-91	0.0034528496898595966
92-93	5.539377980331127E-4
94-95	0.0
96-97	1.5158570777185987E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	889.0
20-21	2533.0
22-23	1606.0
24-25	1951.0
26-27	3270.0
28-29	6836.0
30-31	18938.0
32-33	25267.0
34-35	34080.0
36-37	48374.0
38-39	55426.0
40-41	53520.0
42-43	61783.0
44-45	72554.0
46-47	71345.0
48-49	64005.0
50-51	74555.0
52-53	57496.0
54-55	63055.0
56-57	63929.0
58-59	61420.0
60-61	63564.0
62-63	62224.0
64-65	65094.0
66-67	71628.0
68-69	72972.0
70-71	80058.0
72-73	62045.0
74-75	61237.0
76-77	62947.0
78-79	62826.0
80-81	62693.0
82-83	58698.0
84-85	58380.0
86-87	60111.0
88-89	61104.0
90-91	60699.0
92-93	59788.0
94-95	62683.0
96-97	148695.0
98-99	1017764.0
100-101	2155869.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.56367483630395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.096534466265	79.76747322718182
2	6.407462575063261	11.221219388972521
3	1.5081354538624077	3.9617364747332866
4	0.4935902120365992	1.7288229131662038
5	0.2196637587314632	0.9617282971441081
6	0.10410425623761335	0.5469450745359384
7	0.05532525638173827	0.33911379320319757
8	0.03162183309643813	0.22151391287875064
9	0.01922652472447759	0.15151906432856826
>10	0.06209454716270941	0.8169337221071407
>50	0.0012917722925838392	0.07864656423000202
>100	8.867957877379192E-4	0.1598746193588565
>500	3.127417957008586E-5	0.016759510394760722
>1k	3.127417845008842E-5	0.02771343776486999
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.881853120987536E-5
3	0.0	0.0	0.0	0.0	1.881853120987536E-5
4	0.0	0.0	0.0	0.0	1.881853120987536E-5
5	0.0	0.0	0.0	0.0	1.881853120987536E-5
6	0.0	1.881853120987536E-5	0.0	0.0	1.881853120987536E-5
7	0.0	1.881853120987536E-5	0.0	0.0	1.881853120987536E-5
8	0.0	7.527412483950145E-5	0.0	1.881853120987536E-5	1.881853120987536E-5
9	0.0	1.1291118725925218E-4	0.0	1.881853120987536E-5	1.881853120987536E-5
10-11	0.0	1.1291118725925218E-4	0.0	1.881853120987536E-5	6.586485923456377E-5
12-13	0.0	1.1291118725925218E-4	0.0	1.881853120987536E-5	9.409265604937682E-5
14-15	0.0	1.3172971846912753E-4	0.0	1.881853120987536E-5	9.409265604937682E-5
16-17	0.0	1.3172971846912753E-4	0.0	1.881853120987536E-5	1.035019216543145E-4
18-19	0.0	1.5054824967900292E-4	0.0	1.881853120987536E-5	1.4113898407406523E-4
20-21	0.0	1.6936678088887828E-4	0.0	3.763706241975072E-5	1.6936678088887828E-4
22-23	0.0	1.6936678088887828E-4	0.0	5.645559362962609E-5	1.6936678088887828E-4
24-25	0.0	1.8818531209875364E-4	0.0	1.1291118725925218E-4	2.07003843308629E-4
26-27	0.0	2.07003843308629E-4	0.0	1.6936678088887825E-4	2.07003843308629E-4
28-29	0.0	2.07003843308629E-4	0.0	6.021929987160116E-4	2.07003843308629E-4
30-31	0.0	2.2582237451850435E-4	0.0	0.002239405213975168	2.07003843308629E-4
32-33	0.0	2.2582237451850435E-4	0.0	0.005993702190345304	2.2582237451850435E-4
34-35	0.0	2.728687025431928E-4	0.0	0.011084114882616588	2.2582237451850435E-4
36-37	0.0	3.1991503056788117E-4	0.0	0.01834806792962848	2.2582237451850435E-4
38-39	0.0	3.3873356177775655E-4	0.0	0.028613576704615493	2.2582237451850435E-4
40-41	0.0	3.7637062419750727E-4	0.0	0.039509506275133326	2.2582237451850435E-4
42-43	0.0	3.951891554073826E-4	0.0	0.04953978340999689	2.6345943693825507E-4
44-45	0.0	4.516447490370087E-4	0.0	0.06154600632189738	2.916872337530681E-4
46-47	0.0	4.892818114567595E-4	0.0	0.07502948393377307	3.010964993580058E-4
48-49	0.0	4.892818114567595E-4	0.0	0.0879484056093525	3.010964993580058E-4
50-51	0.0	5.363281394814479E-4	0.0	0.09974762467794437	3.1991503056788117E-4
52-53	0.0	5.457374050863856E-4	0.0	0.11129279357520289	3.1991503056788117E-4
54-55	0.0	5.457374050863856E-4	0.0	0.1237036149081157	3.1991503056788117E-4
56-57	0.0	5.457374050863856E-4	0.0	0.1353616949926335	3.1991503056788117E-4
58-59	0.0	5.457374050863856E-4	0.0	0.1466998600465834	3.1991503056788117E-4
60-61	0.0	5.457374050863856E-4	0.0	0.15754874328907653	3.1991503056788117E-4
62-63	0.0	5.551466706913233E-4	0.0	0.16740965364305121	3.1991503056788117E-4
64-65	0.0	6.774671235555131E-4	0.0	0.17754343269956913	3.1991503056788117E-4
66-67	0.0	6.774671235555131E-4	0.0	0.1873572967255191	3.1991503056788117E-4
68-69	0.0	7.245134515802015E-4	0.0	0.1965407399559383	3.1991503056788117E-4
70-71	0.0	7.339227171851392E-4	0.0	0.20442570453287606	3.3873356177775655E-4
72-73	0.0	7.339227171851392E-4	0.0	0.21074873101939418	3.3873356177775655E-4
74-75	0.0	7.433319827900768E-4	0.0	0.21517108585371492	3.7637062419750727E-4
76-77	0.0	7.621505139999523E-4	0.0	0.21963107775045537	4.14007686617258E-4
78-79	0.0	7.715597796048899E-4	0.0	0.22264204274403543	4.14007686617258E-4
80-81	0.0	7.809690452098275E-4	0.0	0.22379938241344277	4.14007686617258E-4
82-83	0.0	7.997875764197029E-4	0.0	0.2246085792554674	4.14007686617258E-4
84-85	0.0	8.186061076295784E-4	0.0	0.2249849498796649	4.516447490370087E-4
86-87	0.0	8.28015373234516E-4	0.0	0.22513549812934391	4.516447490370087E-4
88	0.0	8.468339044443913E-4	0.0	0.22516372592615871	4.516447490370087E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8940	0.0	48.382595	1
CTTATAC	5760	0.0	34.658688	1
GTATCAA	16785	0.0	33.889587	1
TATACAC	7445	0.0	29.614418	3
ATCAACG	21145	0.0	28.62691	3
TCAACGC	21555	0.0	28.175383	4
TATCAAC	21855	0.0	27.935364	2
CAACGCA	21955	0.0	27.534243	5
AACGCAG	22465	0.0	26.97876	6
TTATACA	8625	0.0	24.58679	2
ACGCAGA	25225	0.0	23.9792	7
CGCAGAG	25715	0.0	23.537865	8
TGGTATC	3980	0.0	23.468107	2
ACACATC	8925	0.0	23.219824	6
GTGGTAT	3840	0.0	22.653418	1
ACATCTC	9080	0.0	22.602722	8
TACACAT	9855	0.0	22.087595	5
GCAGAGT	28470	0.0	21.147503	9
CACATCT	9695	0.0	21.044888	7
GGACCGA	1030	0.0	21.01518	6
>>END_MODULE