FastQCFastQC Report
Fri 27 May 2016
SRR2140040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10861253
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142800.049.8354381
GTATCAA258100.037.6687361
TCAACGC314000.030.3045754
ATCAACG316950.030.0625253
CAACGCA323250.029.450835
TATCAAC331800.028.9776972
AACGCAG333750.028.5629396
ACGCAGA385750.024.6457067
CGCAGAG389150.024.4414278
GTGGTAT66100.022.5733741
TGGTATC65950.021.9075052
GCAGAGT454400.020.7997369
GAGTACT260000.019.21760412-13
GTACATG323750.019.1391491
AGAGTAC413050.018.96767810-11
TACATGG322550.018.8237482
CAGAGTA442700.018.49864610-11
ACATGGG330800.017.7294523
GTACTTT283700.017.46895814-15
AGTACTT280800.017.33467712-13
Produced by FastQC (version 0.11.5)