FastQCFastQC Report
Fri 27 May 2016
SRR2140039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11892148
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160450.049.0066531
GTATCAA285050.037.3227161
CTTATAC106450.037.1352041
ATCAACG346150.030.0120353
TCAACGC346150.029.9875894
CAACGCA356500.029.1412095
TATCAAC365500.028.7432022
AACGCAG366950.028.3347436
TATACAC162550.027.594153
TTATACA164350.025.150642
ACGCAGA420350.024.724967
CGCAGAG425200.024.4328358
ACATCTC181800.022.0022248
GTGGTAT81150.021.708961
TGGTATC75950.021.4414942
ACACATC185450.021.3143356
GCAGAGT495550.020.8516399
TACACAT217900.020.2893875
GTACATG350750.019.9067041
GAGTACT294950.019.65053412-13
Produced by FastQC (version 0.11.5)