FastQCFastQC Report
Fri 27 May 2016
SRR2140038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11297769
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169200.052.006541
GTATCAA305350.037.2416461
ATCAACG392650.028.4037193
TCAACGC395350.028.2427144
TATCAAC401400.028.0103112
CAACGCA403250.027.6684325
AACGCAG413250.026.9885466
GTGGTAT78950.024.5573391
ACGCAGA478050.023.3212787
TGGTATC79800.023.2231032
CGCAGAG482350.023.0690148
GCAGAGT549600.020.1140469
GTACATG381200.020.0185991
GAGTACT318450.019.7089112-13
TACATGG380550.019.5916792
AGAGTAC499350.019.00138710-11
ACATGGG385800.018.7025893
CAGAGTA535300.018.36089510-11
AGTACTT344000.017.81584212-13
GTACTTT352250.017.80638714-15
Produced by FastQC (version 0.11.5)