##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140038_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11297769
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64965445832713	34.0	31.0	34.0	31.0	34.0
2	32.80593009115339	34.0	31.0	34.0	31.0	34.0
3	32.85135428065488	34.0	31.0	34.0	31.0	34.0
4	36.31812643717534	37.0	37.0	37.0	35.0	37.0
5	36.25180555559243	37.0	37.0	37.0	35.0	37.0
6	36.288381183931094	37.0	37.0	37.0	35.0	37.0
7	36.24377051787835	37.0	37.0	37.0	35.0	37.0
8	36.22439412595531	37.0	37.0	37.0	35.0	37.0
9	37.89891260832116	39.0	38.0	39.0	35.0	39.0
10-11	37.87171037927931	39.0	38.0	39.0	35.0	39.0
12-13	37.84420393088228	39.0	38.0	39.0	35.0	39.0
14-15	39.25353775599413	41.0	39.0	41.0	36.0	41.0
16-17	39.238655924014736	41.0	39.0	41.0	36.0	41.0
18-19	39.27019347802208	41.0	39.0	41.0	36.0	41.0
20-21	39.1900831608031	41.0	39.0	41.0	36.0	41.0
22-23	39.186829159814664	40.0	39.0	41.0	36.0	41.0
24-25	39.092968516391835	40.0	39.0	41.0	35.5	41.0
26-27	38.94922305368702	40.0	39.0	41.0	35.0	41.0
28-29	38.81724778293541	40.0	38.0	41.0	35.0	41.0
30-31	38.697399344343495	40.0	38.0	41.0	35.0	41.0
32-33	38.59790557364267	40.0	38.0	41.0	35.0	41.0
34-35	38.45094848151195	40.0	38.0	41.0	34.5	41.0
36-37	38.591684162824095	40.0	38.0	41.0	35.0	41.0
38-39	38.65361238977867	40.0	38.0	41.0	35.0	41.0
40-41	38.63295091995725	40.0	38.0	41.0	35.0	41.0
42-43	38.59475513934282	40.0	38.0	41.0	34.5	41.0
44-45	38.51409965398216	40.0	38.0	41.0	34.0	41.0
46-47	38.444667034709646	40.0	38.0	41.0	34.0	41.0
48-49	38.35014895479188	40.0	38.0	41.0	34.0	41.0
50-51	38.24897398799525	40.0	38.0	41.0	34.0	41.0
52-53	38.13127054246374	40.0	37.0	41.0	33.5	41.0
54-55	37.982639456500635	40.0	37.0	41.0	33.0	41.0
56-57	37.82668760950105	40.0	36.5	41.0	33.0	41.0
58-59	37.632952634920144	39.0	36.0	41.0	33.0	41.0
60-61	37.394774077718836	39.0	36.0	41.0	33.0	41.0
62-63	37.182517400804784	39.0	35.0	40.0	32.0	41.0
64-65	36.9324740878389	38.0	35.0	40.0	32.0	41.0
66-67	36.646648079959775	38.0	35.0	40.0	32.0	41.0
68-69	36.284558099380334	37.0	35.0	40.0	31.0	41.0
70-71	36.00487766628194	36.5	35.0	39.0	31.0	41.0
72-73	35.67889686010274	36.0	34.0	39.0	31.0	40.0
74-75	35.35133728901439	36.0	34.0	38.5	31.0	40.0
76-77	34.337040729279444	35.0	33.5	37.0	29.5	39.0
78-79	34.63538794907382	35.0	34.0	37.0	30.0	39.0
80-81	34.48090808478007	35.0	34.0	36.5	30.0	39.0
82-83	34.21878813011755	35.0	34.0	36.0	30.0	37.5
84-85	33.96555556530613	35.0	34.0	36.0	30.0	37.0
86-87	33.751708976336914	35.0	33.0	35.0	30.0	37.0
88-89	33.58159504896626	35.0	33.0	35.0	30.0	36.0
90-91	33.40376044368516	35.0	33.0	35.0	29.5	36.0
92-93	33.2728640121765	34.5	33.0	35.0	29.0	36.0
94-95	33.18921356942239	34.0	33.0	35.0	29.0	35.0
96-97	33.17617865776273	34.0	33.0	35.0	29.0	35.0
98-99	33.188515636598396	34.0	33.0	35.0	29.5	35.0
100-101	32.18710282071058	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	5.0
20	60.0
21	298.0
22	1417.0
23	4823.0
24	12251.0
25	25297.0
26	45054.0
27	70536.0
28	101127.0
29	135294.0
30	174460.0
31	218046.0
32	276354.0
33	362675.0
34	513328.0
35	753740.0
36	1309957.0
37	2648702.0
38	3247486.0
39	1345529.0
40	51326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.196508620418776	21.00436820756381	15.812449342874684	23.986673829142727
2	13.827506902126348	21.327161010193876	41.34509339347737	23.50023869420241
3	17.166442330339734	26.157031534279024	31.085809950619453	25.590716184761785
4	12.16391699350854	18.620688495346027	38.4972003759681	30.718194135177335
5	13.228921568497285	37.81630691864916	35.28931242973724	13.665459083116321
6	29.965040000375293	37.98531373760607	18.484835368823703	13.564810893194931
7	26.5480821921567	32.95587828003918	22.562029724629703	17.934009803174416
8	24.836717762595427	36.62006189009529	20.604961917702514	17.938258429606766
9	25.557630006419853	17.10717399160843	21.06088379041915	36.27431221155256
10-11	24.27706731483511	27.03367297384627	28.364760359438385	20.324499351880238
12-13	25.170019194498455	24.469518029157705	28.68910351632714	21.6713592600167
14-15	22.281633601783586	25.879777504880202	26.58375794830906	25.25483094502715
16-17	21.610940761455584	29.117894845294757	27.194176537869108	22.076987855380548
18-19	21.612273326908465	27.987678628203376	29.322402442677177	21.077645602210985
20-21	22.518553177454866	27.042385272847508	29.23097023020625	21.208091319491377
22-23	22.234104543228376	26.97653749382272	29.125721384039334	21.66363657890957
24-25	21.964107938716474	27.18817953282386	29.196350451213004	21.65136207724666
26-27	21.918613872937495	27.334495974995864	29.079016565141114	21.66787358692552
28-29	21.746018359931096	27.393010614995056	29.1654276496844	21.695543375389448
30-31	22.11636058006747	27.172122572524675	29.08352368909057	21.62799315831728
32-33	21.889902848897663	27.40028501461636	28.950101039286714	21.759711097199258
34-35	21.98700026845369	27.444996017847618	29.014958925513657	21.553044788185037
36-37	22.01010078669623	27.372201597031765	28.93728448725506	21.680413129016944
38-39	21.855210720005143	27.45362427822003	28.966097710964643	21.72506729081018
40-41	22.103428894672984	27.323877895135396	28.815106720151668	21.75758649003995
42-43	22.0252839933751	27.40925091613083	28.63317698891122	21.93228810158285
44-45	22.139036754465728	27.55829877737718	28.353940298674924	21.94872416948217
46-47	22.22212418554547	27.55843335512379	27.996561327444258	22.222881131886478
48-49	22.157706098566827	27.726084246069878	27.937290571580487	22.178919083782805
50-51	22.14831534772012	27.78503800796678	27.85941755370514	22.207229090607957
52-53	22.31185622090744	27.744852719610126	27.75842877044693	22.184862289035504
54-55	22.26124985229709	27.672806166240814	27.73683354804527	22.329110433416822
56-57	22.33881016519779	27.775616094614364	27.661666238064946	22.2239075021229
58-59	22.242823295874672	27.877884926015394	27.755130471361788	22.124161306748142
60-61	22.419551557709077	27.80484766871117	27.662028707142568	22.113572066437186
62-63	22.317673488608776	27.913520309342466	27.566130613346772	22.20267558870199
64-65	22.28742043200171	27.866935943267585	27.580498904902573	22.265144719828132
66-67	22.39665617304762	27.842448408281427	27.459200116066658	22.301695302604298
68-69	22.304915541317914	27.78979365201976	27.540436483718008	22.364854322944318
70-71	22.295373545371806	27.603542351778653	27.607509970445363	22.493574132404177
72-73	22.352014577173467	27.654569645966504	27.56168813550045	22.43172764135958
74-75	22.205781180242923	27.765346169497825	27.558397056013995	22.47047559424526
76-77	22.34828720711267	27.662039499320528	27.490631557810058	22.499041735756744
78-79	22.316076828843524	27.67396380515535	27.474776485132836	22.535182880868295
80-81	22.345715416207753	27.694288119429533	27.47635650951525	22.48363995484746
82-83	22.353706212315753	27.648132394604392	27.601419174373977	22.396742218705885
84-85	22.456290872922345	27.628511946460364	27.51528819852322	22.399908982094065
86-87	22.278879002001418	27.66100333726605	27.588211769134503	22.47190589159803
88-89	22.3357987213319	27.569448536771272	27.578832975498386	22.51591976639844
90-91	22.413029417496436	27.54256168870747	27.591897230130503	22.452511663665593
92-93	22.426591186625167	27.441856008902256	27.647505666874313	22.484047137598267
94-95	22.2647442926678	27.51643596295288	27.69220277870035	22.52661696567897
96-97	22.249458168543462	27.571092380955665	27.68548456491634	22.493964885584536
98-99	22.453917672851674	28.167823844649902	27.713144338597957	21.66511414390047
100-101	22.988979883776185	30.80718621521143	24.934994092165045	21.26883980884734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2931.0
1	2511.5
2	3041.0
3	4226.0
4	5646.5
5	8490.0
6	11004.0
7	13939.5
8	16757.5
9	17528.5
10	16149.5
11	14635.5
12	16108.0
13	21917.5
14	36246.5
15	55896.0
16	71415.5
17	79384.5
18	80107.0
19	75417.5
20	67965.5
21	59986.0
22	54592.5
23	55342.5
24	62136.0
25	75300.0
26	92910.5
27	112908.5
28	137329.5
29	164074.5
30	188910.5
31	214555.0
32	241477.0
33	267532.5
34	291721.0
35	312566.5
36	336660.0
37	360879.5
38	378043.5
39	389032.0
40	401623.5
41	416899.0
42	426780.0
43	435402.5
44	445885.0
45	456107.0
46	463657.5
47	466001.5
48	461186.0
49	451751.5
50	443789.5
51	434492.5
52	417592.0
53	397280.0
54	377199.5
55	352724.0
56	325598.5
57	295581.0
58	263460.5
59	229670.5
60	192117.5
61	156811.0
62	125096.5
63	96781.0
64	72856.5
65	54121.5
66	40892.5
67	31495.5
68	24202.5
69	17682.5
70	12593.0
71	9149.0
72	6541.5
73	4498.5
74	3013.0
75	1987.5
76	1277.0
77	813.5
78	497.5
79	290.5
80	197.0
81	135.5
82	102.0
83	77.5
84	54.5
85	39.5
86	30.0
87	25.0
88	17.0
89	8.5
90	4.5
91	3.0
92	2.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007859958899850049
3	0.0
4	1.770261013479741E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.833404984647854E-4
12-13	4.425652533699352E-6
14-15	7.346583205940925E-4
16-17	8.054687611332821E-4
18-19	8.851305067398704E-6
20-21	4.5585538496876614E-4
22-23	0.001301279427670751
24-25	0.004400437511507432
26-27	0.016478843574012726
28-29	0.32251384792892906
30-31	0.30960455496158457
32-33	0.31834505905560195
34-35	0.1832943127951946
36-37	0.04438456888433179
38-39	0.0345213470344804
40-41	0.03083328654763177
42-43	0.02647725461139819
44-45	0.014280486033077763
46-47	0.007327446641631069
48-49	0.0055783523178569525
50-51	0.0021647873980257517
52-53	0.0019808927655129618
54-55	0.0014248360143278055
56-57	6.045690133907684E-4
58-59	0.0021169158993700125
60-61	0.0024497830146508336
62-63	3.0755965255878967E-4
64-65	1.9513725954836632E-4
66-67	0.00783449149275754
68-69	0.007025017377607438
70-71	0.00900104663572615
72-73	0.009629572056823528
74-75	0.007600391081353388
76-77	0.004816952361985694
78-79	5.038620490032643E-4
80-81	7.532953419576076E-4
82-83	7.014606108768373E-4
84-85	0.0
86-87	0.0016819766724412665
88-89	0.0013965757325550658
90-91	0.0010359665723515027
92-93	0.011349694183278811
94-95	9.496087730556107E-5
96-97	5.442348656447387E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	208.0
20-21	677.0
22-23	1570.0
24-25	3904.0
26-27	5908.0
28-29	9450.0
30-31	20629.0
32-33	27822.0
34-35	41579.0
36-37	57104.0
38-39	68115.0
40-41	72682.0
42-43	87503.0
44-45	105863.0
46-47	104962.0
48-49	91302.0
50-51	79286.0
52-53	76674.0
54-55	82795.0
56-57	87310.0
58-59	86638.0
60-61	85533.0
62-63	85099.0
64-65	92098.0
66-67	98047.0
68-69	96745.0
70-71	94637.0
72-73	91206.0
74-75	93983.0
76-77	95365.0
78-79	101224.0
80-81	102851.0
82-83	101125.0
84-85	103373.0
86-87	108423.0
88-89	117537.0
90-91	126730.0
92-93	131420.0
94-95	147601.0
96-97	220632.0
98-99	958629.0
100-101	7133530.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.79210171188221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.653087252247	73.39775416860402
2	7.635628215983395	12.6433941578363
3	1.9433740465697407	4.8268806518350225
4	0.7937853279792206	2.628766224458217
5	0.362703599204109	1.5014496638286174
6	0.1991638184933512	0.989351467081703
7	0.12380439309103465	0.7175018133619552
8	0.07263404116945472	0.48108199393972284
9	0.049621071465222516	0.36974095162211507
>10	0.16175845384631152	2.079543851835377
>50	0.0035355265733510713	0.19699259344955733
>100	8.467721494940988E-4	0.11877896234172249
>500	3.832111834892151E-5	0.02549239413920673
>1k	1.916010994538821E-5	0.023271105666576284
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.851305067398704E-6	0.0
2	0.0	4.425652533699352E-5	0.0	8.851305067398704E-6	0.0
3	0.0	4.425652533699352E-5	0.0	1.770261013479741E-5	0.0
4	0.0	7.081044053918964E-5	0.0	1.770261013479741E-5	0.0
5	0.0	7.966174560658835E-5	0.0	1.770261013479741E-5	0.0
6	0.0	7.966174560658835E-5	0.0	2.6553915202196113E-5	0.0
7	0.0	7.966174560658835E-5	0.0	4.425652533699352E-5	0.0
8	0.0	1.1506696587618316E-4	0.0	4.425652533699352E-5	0.0
9	0.0	1.947287114827715E-4	0.0	5.310783040439223E-5	0.0
10-11	0.0	1.947287114827715E-4	0.0	5.310783040439223E-5	0.0
12-13	0.0	1.947287114827715E-4	0.0	7.523609307288899E-5	0.0
14-15	0.0	2.25708279218667E-4	0.0	8.851305067398704E-5	0.0
16-17	0.0	2.3013393175236633E-4	0.0	1.0621566080878445E-4	0.0
18-19	0.0	2.655391520219612E-4	0.0	1.2391827094358188E-4	0.0
20-21	4.425652533699352E-6	2.965187197578566E-4	0.0	1.3719522854467993E-4	0.0
22-23	8.851305067398704E-6	3.452008976285495E-4	0.0	1.4162088107837927E-4	0.0
24-25	8.851305067398704E-6	3.717548128307456E-4	0.0	1.593234912131767E-4	0.0
26-27	8.851305067398704E-6	3.89457422965543E-4	0.0	2.124313216175689E-4	0.0
28-29	8.851305067398704E-6	3.9388307549924237E-4	0.0	5.045243888417262E-4	0.0
30-31	8.851305067398704E-6	4.51416558437334E-4	0.0	0.0012126287942336225	0.0
32-33	8.851305067398704E-6	4.735448211058307E-4	0.0	0.0027394789183598995	0.0
34-35	8.851305067398704E-6	5.886117869820139E-4	0.0	0.004762002126260503	0.0
36-37	8.851305067398704E-6	7.169557104592952E-4	0.0	0.00792634368785554	0.0
38-39	8.851305067398704E-6	7.78914845931086E-4	0.0	0.013037972364278293	0.0
40-41	8.851305067398704E-6	8.497252864702756E-4	0.0	0.018202708871105436	0.0
42-43	8.851305067398704E-6	8.807048542061711E-4	0.0	0.023216973191786804	0.0
44-45	8.851305067398704E-6	0.001004623125149753	0.0	0.029430589349100693	0.0
46-47	8.851305067398704E-6	0.0011064131334248381	0.0	0.03551143593040361	0.0
48-49	8.851305067398704E-6	0.0011152644384922368	0.0	0.042158766036020034	0.0
50-51	8.851305067398704E-6	0.0012303314043684201	0.0	0.04887690658217565	0.0
52-53	8.851305067398704E-6	0.0012480340145032175	0.0	0.055046266214152546	0.0
54-55	8.851305067398704E-6	0.0012524596670369168	0.0	0.061963561124324634	0.0
56-57	8.851305067398704E-6	0.0012568853195706162	0.0	0.06896494343263701	0.0
58-59	8.851305067398704E-6	0.0012613109721043155	0.0	0.07493957435313114	0.0
60-61	8.851305067398704E-6	0.001279013582239113	0.0	0.08055572741839562	0.0
62-63	8.851305067398704E-6	0.0013099931499750083	0.0	0.08603468525511541	0.0
64-65	8.851305067398704E-6	0.0015357014291936754	0.0	0.09168624354064948	0.0
66-67	8.851305067398704E-6	0.0015534040393284728	0.0	0.0977803670795535	0.0
68-69	8.851305067398704E-6	0.001593234912131767	0.0	0.10398955758433368	0.0
70-71	8.851305067398704E-6	0.001619788827333963	0.0	0.10955260281919377	0.0
72-73	8.851305067398704E-6	0.0016330657849350611	0.0	0.1133940692184448	0.0
74-75	8.851305067398704E-6	0.0016817479628057539	0.0	0.11689476037260099	0.0
76-77	8.851305067398704E-6	0.0017525584033449438	0.0	0.11982896800244366	0.0
78-79	8.851305067398704E-6	0.0018233688438841333	0.0	0.12254189300560137	0.0
80-81	8.851305067398704E-6	0.0018233688438841333	0.0	0.12378107571503719	0.0
82-83	8.851305067398704E-6	0.0018277944964178326	0.0	0.12418823574813753	0.0
84-85	8.851305067398704E-6	0.001832220148951532	0.0	0.12445820055269319	0.0
86-87	8.851305067398704E-6	0.0018410714540189306	0.0	0.12453786229829977	0.0
88-89	8.851305067398704E-6	0.0019915436401647087	0.0	0.12453786229829977	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16920	0.0	52.00654	1
GTATCAA	30535	0.0	37.241646	1
ATCAACG	39265	0.0	28.403719	3
TCAACGC	39535	0.0	28.242714	4
TATCAAC	40140	0.0	28.010311	2
CAACGCA	40325	0.0	27.668432	5
AACGCAG	41325	0.0	26.988546	6
GTGGTAT	7895	0.0	24.557339	1
ACGCAGA	47805	0.0	23.321278	7
TGGTATC	7980	0.0	23.223103	2
CGCAGAG	48235	0.0	23.069014	8
GCAGAGT	54960	0.0	20.114046	9
GTACATG	38120	0.0	20.018599	1
GAGTACT	31845	0.0	19.70891	12-13
TACATGG	38055	0.0	19.591679	2
AGAGTAC	49935	0.0	19.001387	10-11
ACATGGG	38580	0.0	18.702589	3
CAGAGTA	53530	0.0	18.360895	10-11
AGTACTT	34400	0.0	17.815842	12-13
GTACTTT	35225	0.0	17.806387	14-15
>>END_MODULE