FastQCFastQC Report
Fri 27 May 2016
SRR2140037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10713396
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158400.050.6891671
GTATCAA285250.037.635841
ATCAACG359100.029.3069863
TCAACGC359100.029.2833354
CAACGCA366050.028.7509525
TATCAAC373350.028.398152
AACGCAG377450.027.9850226
GTGGTAT74600.025.10781
TGGTATC72700.024.5821532
ACGCAGA434100.024.263717
CGCAGAG437550.024.0527618
GCAGAGT502550.020.8564879
GTACATG340400.020.1294611
TACATGG333600.020.1019342
GAGTACT299850.020.01340312-13
ACATGGG340600.019.371673
AGAGTAC458600.019.21631610-11
CAGAGTA492700.018.60563510-11
AGTACTT326150.017.91883512-13
GTACTTT334200.017.89264714-15
Produced by FastQC (version 0.11.5)