##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10713396
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64840130991144	34.0	31.0	34.0	31.0	34.0
2	32.80371042011328	34.0	31.0	34.0	31.0	34.0
3	32.84904357124482	34.0	31.0	34.0	31.0	34.0
4	36.32009542072374	37.0	37.0	37.0	35.0	37.0
5	36.25290860153027	37.0	37.0	37.0	35.0	37.0
6	36.289268874220646	37.0	37.0	37.0	35.0	37.0
7	36.24463624792736	37.0	37.0	37.0	35.0	37.0
8	36.224077780752246	37.0	37.0	37.0	35.0	37.0
9	37.90158153399725	39.0	38.0	39.0	35.0	39.0
10-11	37.87193491214177	39.0	38.0	39.0	35.0	39.0
12-13	37.84683736137449	39.0	38.0	39.0	35.0	39.0
14-15	39.258195253867214	41.0	39.0	41.0	36.0	41.0
16-17	39.24277045299175	41.0	39.0	41.0	36.0	41.0
18-19	39.26998754643252	41.0	39.0	41.0	36.0	41.0
20-21	39.18972710605382	40.5	39.0	41.0	36.0	41.0
22-23	39.18689665610434	40.0	39.0	41.0	36.0	41.0
24-25	39.09565104033618	40.0	39.0	41.0	35.5	41.0
26-27	38.955909173258135	40.0	39.0	41.0	35.0	41.0
28-29	38.826219139989675	40.0	38.0	41.0	35.0	41.0
30-31	38.707156916802404	40.0	38.0	41.0	35.0	41.0
32-33	38.611247563651325	40.0	38.0	41.0	35.0	41.0
34-35	38.466993332687835	40.0	38.0	41.0	34.5	41.0
36-37	38.611705830391955	40.0	38.0	41.0	35.0	41.0
38-39	38.67250418256166	40.0	38.0	41.0	35.0	41.0
40-41	38.65288406726458	40.0	38.0	41.0	35.0	41.0
42-43	38.61586139837448	40.0	38.0	41.0	35.0	41.0
44-45	38.53952751363299	40.0	38.0	41.0	34.0	41.0
46-47	38.47043528294757	40.0	38.0	41.0	34.0	41.0
48-49	38.37896101935296	40.0	38.0	41.0	34.0	41.0
50-51	38.282170184610955	40.0	38.0	41.0	34.0	41.0
52-53	38.17060001923171	40.0	37.0	41.0	33.5	41.0
54-55	38.02625346120024	40.0	37.0	41.0	33.0	41.0
56-57	37.87520611502951	40.0	37.0	41.0	33.0	41.0
58-59	37.6864351701219	39.0	36.0	41.0	33.0	41.0
60-61	37.45632574641333	39.0	36.0	41.0	33.0	41.0
62-63	37.25114826338218	39.0	35.0	40.0	32.5	41.0
64-65	37.00883115847289	38.0	35.0	40.0	32.0	41.0
66-67	36.72527289946167	38.0	35.0	40.0	32.0	41.0
68-69	36.3658969592948	37.0	35.0	40.0	31.0	41.0
70-71	36.08581076252167	37.0	35.0	39.0	31.0	41.0
72-73	35.7576049762452	36.0	34.5	39.0	31.0	40.0
74-75	35.42567628088786	36.0	34.0	38.5	31.0	40.0
76-77	34.40605446040537	35.0	33.5	37.0	29.5	39.0
78-79	34.70090436800743	35.0	34.0	37.0	30.0	39.0
80-81	34.538159286696	35.0	34.0	37.0	30.0	39.0
82-83	34.264496070147764	35.0	34.0	36.0	30.0	38.0
84-85	34.00550317208871	35.0	34.0	36.0	30.0	37.0
86-87	33.78231129261013	35.0	33.5	35.0	30.0	37.0
88-89	33.60198128567699	35.0	33.0	35.0	30.0	36.5
90-91	33.41606729152299	35.0	33.0	35.0	29.5	36.0
92-93	33.282084802972136	34.5	33.0	35.0	29.0	36.0
94-95	33.193488303314716	34.0	33.0	35.0	29.0	35.5
96-97	33.177422243568	34.0	33.0	35.0	29.0	35.0
98-99	33.189820746851005	34.0	33.0	35.0	29.5	35.0
100-101	32.191478841642066	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	0.0
19	5.0
20	39.0
21	218.0
22	1231.0
23	4077.0
24	10396.0
25	22186.0
26	39607.0
27	63823.0
28	92968.0
29	125831.0
30	163242.0
31	206559.0
32	263647.0
33	344927.0
34	486421.0
35	711436.0
36	1229595.0
37	2486824.0
38	3135848.0
39	1277153.0
40	47360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.84587109446902	21.178718680799253	15.83189868086646	24.14351154386527
2	13.711875711493915	21.367664582340527	41.445741631618034	23.474718074547525
3	17.034150515858837	26.07816419742162	31.25632619199365	25.63135909472589
4	12.032742568110775	18.640359781443657	38.69516408107124	30.63173356937433
5	13.069702641440678	37.84417191336902	35.5915808582078	13.494544586982505
6	29.9105717738801	38.23501903598075	18.423177860689552	13.431231329449597
7	26.377023681379836	32.964029332995814	22.705620141363205	17.95332684426115
8	24.55965410034316	36.95928909936681	20.716586972048827	17.764469828241204
9	25.36429158410648	17.06883606281332	21.195212050408667	36.37166030267153
10-11	24.122871456160606	27.136113862102373	28.55882542370192	20.1821892580351
12-13	24.980431041660367	24.535394752513582	28.859065790156546	21.6251084156695
14-15	21.998940569199032	26.005362493349388	26.717505343824964	25.278191593626616
16-17	21.449830691962422	29.247413941887068	27.37444990172508	21.928305464425428
18-19	21.42861543223371	28.17055920840772	29.422982110057745	20.97784324930083
20-21	22.30812951774446	27.243188064184242	29.37377964076283	21.074902777308463
22-23	21.97897483351284	27.162358674077247	29.277953914534066	21.58071257787585
24-25	21.76973990709237	27.351504117528062	29.334928868747372	21.543827106632197
26-27	21.750001529645676	27.502095147510857	29.22877320034617	21.519130122497295
28-29	21.543001468961815	27.577294869639836	29.294336767348376	21.58536689404997
30-31	21.945615312578003	27.360610911656902	29.252101463282802	21.44167231248229
32-33	21.658890180660503	27.553747075296915	29.121895654209716	21.66546708983287
34-35	21.813261641490257	27.61816844036818	29.176114979355326	21.39245493878623
36-37	21.80072985290759	27.522438542931443	29.109386991235358	21.567444612925605
38-39	21.64457978627963	27.630610980584237	29.157400608089397	21.567408625046745
40-41	21.89788292875523	27.52193572800194	29.005038754805927	21.575142588436904
42-43	21.821878674189627	27.61761094356036	28.776492181932262	21.78401820031775
44-45	21.919476750733658	27.744369114904956	28.549916456141954	21.78623767821943
46-47	22.009440515596637	27.71768654452168	28.2048094418067	22.068063498074984
48-49	21.96009700597484	27.87468651564531	28.17975394899343	21.98546252938642
50-51	21.94454629175424	27.997878811746485	28.044370609083842	22.013204287415437
52-53	22.144308648206255	27.910870319228152	27.932930880932556	22.011890151633036
54-55	22.09265939755062	27.811837229077103	27.971955995607335	22.123547377764947
56-57	22.108409402774647	27.95147558325219	27.88845401900985	22.051660994963314
58-59	22.033966726154105	28.02970459951849	27.987412641074073	21.94891603325333
60-61	22.184301453287954	28.027267786475157	27.880827605267065	21.90760315496982
62-63	22.108735506530834	28.12370638247556	27.79125775753429	21.97630035345932
64-65	22.06902377957004	28.061894518968895	27.799684673183165	22.0693970282779
66-67	22.16081217604024	28.020711140573408	27.694767687560347	22.12370899582601
68-69	22.10316018304773	27.967133599217348	27.79711188597527	22.132594331759655
70-71	22.09234796034389	27.789832882449883	27.832706693219443	22.285112463986785
72-73	22.138426915756444	27.857430200042906	27.790942094050568	22.21320079015009
74-75	22.020681589102033	27.924486247668177	27.784143233608592	22.270688929621198
76-77	22.140299814619326	27.86436612366416	27.72289198302822	22.272442078688297
78-79	22.090217321948693	27.905081390134328	27.685496981739455	22.319204306177525
80-81	22.138224344042836	27.888642791302924	27.707157647812668	22.265975216841564
82-83	22.129999220195412	27.84967938211016	27.81989302733625	22.200428370358175
84-85	22.23294399718829	27.81442243058733	27.7769039511848	22.17572962103958
86-87	22.065634909948056	27.84433543322381	27.822389568078158	22.26764008874997
88-89	22.14332899957802	27.774124269498678	27.79064370660791	22.29190302431539
90-91	22.16665086799658	27.770266726461024	27.856620091866457	22.20646231367594
92-93	22.213506365399624	27.66496967835864	27.877602274704373	22.24392168153736
94-95	22.055873194819725	27.75563784389351	27.913129245200118	22.275359716086644
96-97	22.01316245420886	27.77365304203871	27.955533378395852	22.257651125356578
98-99	22.2322576693626	28.359124388766183	27.997867964773942	21.41074997709728
100-101	22.76938852561852	31.061768858188422	25.212002661969535	20.956839954223526
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2780.0
1	2321.5
2	2672.0
3	3702.5
4	4997.0
5	7605.5
6	9941.0
7	12690.5
8	15282.0
9	15923.0
10	14698.0
11	13416.0
12	14905.5
13	20441.5
14	33867.5
15	52098.0
16	66261.5
17	73486.0
18	74324.5
19	70269.5
20	63447.5
21	55961.5
22	51610.5
23	53357.0
24	60946.0
25	74756.0
26	93190.0
27	113547.0
28	135975.5
29	159708.0
30	182108.0
31	205147.0
32	230505.5
33	256822.5
34	281837.5
35	303375.5
36	326642.0
37	348478.0
38	365400.0
39	379239.0
40	394048.0
41	408739.0
42	417855.5
43	425837.5
44	433677.0
45	440581.5
46	445377.0
47	445971.5
48	440273.0
49	429527.0
50	418846.0
51	404606.0
52	383501.0
53	360652.0
54	338381.5
55	314705.0
56	289825.0
57	262842.5
58	233575.0
59	202146.5
60	168892.0
61	137930.5
62	109642.5
63	84797.0
64	63497.0
65	46961.0
66	35988.5
67	27967.5
68	21436.0
69	15818.5
70	11477.0
71	8231.0
72	5728.0
73	4006.5
74	2763.5
75	1832.5
76	1161.5
77	739.0
78	476.5
79	312.5
80	210.5
81	137.0
82	87.0
83	55.0
84	40.0
85	31.5
86	23.5
87	14.5
88	4.5
89	2.0
90	3.5
91	4.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008410031702365898
3	0.0
4	2.8002325313094E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.02733325642028E-4
12-13	0.0
14-15	8.96074410019008E-4
16-17	7.747310003289339E-4
18-19	1.8668216875396E-5
20-21	5.18057839430913E-4
22-23	0.0012649013311616239
24-25	0.0043836086090151645
26-27	0.016788253471525953
28-29	0.32080041521174657
30-31	0.3084053363627553
32-33	0.3167209296603984
34-35	0.18284738100835352
36-37	0.04446629338441469
38-39	0.03416642692306853
40-41	0.030108527164333106
42-43	0.026202381914198557
44-45	0.013604451140066568
46-47	0.0069489838468156425
48-49	0.004940578804847828
50-51	0.0018409298307702864
52-53	0.0015084345546374541
54-55	0.0011509034870003179
56-57	4.1719114461936116E-4
58-59	0.00209788333829764
60-61	0.001908299042561378
62-63	2.0332889016261982E-4
64-65	1.3142542863352145E-4
66-67	0.007984531336659354
68-69	0.007171883261022646
70-71	0.00956565543611494
72-73	0.010053261546569305
74-75	0.007981661018360795
76-77	0.005060535122115717
78-79	3.875598674680056E-4
80-81	5.676522889045889E-4
82-83	6.713504357465991E-4
84-85	0.0
86-87	0.0017417980810311444
88-89	0.0013652240976773917
90-91	0.001130754971412349
92-93	0.011868131814489079
94-95	1.3013752747993324E-4
96-97	2.5242745282336E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	182.0
20-21	647.0
22-23	1399.0
24-25	3294.0
26-27	5058.0
28-29	8558.0
30-31	19101.0
32-33	25628.0
34-35	37981.0
36-37	53167.0
38-39	63461.0
40-41	66953.0
42-43	80354.0
44-45	97816.0
46-47	97664.0
48-49	84098.0
50-51	73518.0
52-53	70690.0
54-55	76092.0
56-57	80136.0
58-59	79491.0
60-61	78480.0
62-63	78084.0
64-65	84587.0
66-67	89814.0
68-69	88666.0
70-71	86323.0
72-73	83893.0
74-75	85787.0
76-77	87713.0
78-79	92697.0
80-81	95041.0
82-83	92638.0
84-85	94350.0
86-87	100012.0
88-89	108197.0
90-91	117997.0
92-93	122039.0
94-95	137236.0
96-97	207765.0
98-99	915338.0
100-101	6841451.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.77002771817777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.08052085399687	74.6227770108903
2	7.438741145795241	12.462871039432338
3	1.8804829087949055	4.725843161799265
4	0.7093096714859048	2.3767556336458333
5	0.34641623903985924	1.4509648973197955
6	0.17795733186612783	0.8944494373847095
7	0.11309034055216542	0.6631506673899142
8	0.07077144600085429	0.47428207945176687
9	0.042643891089619385	0.32150519447295334
>10	0.1367279266922998	1.736578631602649
>50	0.0026778481546171893	0.14605580001276897
>100	6.225532385932213E-4	0.09793158840969453
>500	1.8922390457234663E-5	0.009304708246077806
>1k	1.8920902364621594E-5	0.017530149941842384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.8668216875396E-5	0.0
5	0.0	0.0	0.0	1.8668216875396E-5	0.0
6	5.6004650626188E-5	0.0	0.0	1.8668216875396E-5	0.0
7	5.6004650626188E-5	0.0	0.0	1.8668216875396E-5	0.0
8	5.6004650626188E-5	1.8668216875396E-5	0.0	1.8668216875396E-5	9.334108437698E-6
9	5.6004650626188E-5	5.6004650626188E-5	0.0	3.7336433750792E-5	9.334108437698E-6
10-11	5.6004650626188E-5	5.6004650626188E-5	0.0	4.667054218849E-5	9.334108437698E-6
12-13	5.6004650626188E-5	5.6004650626188E-5	0.0	5.1337596407339E-5	2.3335271094245E-5
14-15	5.6004650626188E-5	9.334108437698E-5	0.0	7.933992172043299E-5	4.2003487969640997E-5
16-17	1.21343409690074E-4	9.334108437698E-5	0.0	1.30677518127772E-4	6.0671704845036994E-5
18-19	1.21343409690074E-4	1.02675192814678E-4	0.0	1.58679843440866E-4	1.02675192814678E-4
20-21	1.26010463908923E-4	1.30677518127772E-4	0.0	1.6801395187856399E-4	1.4001162656547E-4
22-23	1.30677518127772E-4	1.6801395187856399E-4	0.0	1.82015114535111E-4	1.5401278922201698E-4
24-25	1.35344572346621E-4	1.91349222972809E-4	0.0	2.28685656723601E-4	1.77348060316262E-4
26-27	1.4001162656547E-4	1.96016277191658E-4	0.0	3.03358524225185E-4	1.77348060316262E-4
28-29	1.4001162656547E-4	1.96016277191658E-4	0.0	5.08708909854541E-4	1.82015114535111E-4
30-31	1.4001162656547E-4	2.24018602504752E-4	0.0	0.001339444560809663	1.8668216875396E-4
32-33	1.4001162656547E-4	2.24018602504752E-4	0.0	0.0029262429952183226	1.96016277191658E-4
34-35	1.49345735003168E-4	2.70689144693242E-4	0.0	0.0051850972371412386	1.96016277191658E-4
36-37	1.49345735003168E-4	3.12692632662883E-4	0.0	0.008708723172372233	1.96016277191658E-4
38-39	1.49345735003168E-4	3.17359686881732E-4	0.0	0.01330110452371965	2.00683331410507E-4
40-41	1.49345735003168E-4	3.64030229070222E-4	0.0	0.018322854863201173	2.05350385629356E-4
42-43	1.58679843440866E-4	3.82698445945618E-4	0.0	0.02319992652189838	2.10017439848205E-4
44-45	1.58679843440866E-4	4.52704259228353E-4	0.0	0.028842395072486822	2.1468449406705399E-4
46-47	1.58679843440866E-4	5.04041855635692E-4	0.0	0.03539027214153197	2.1468449406705399E-4
48-49	1.58679843440866E-4	5.04041855635692E-4	0.0	0.042218172463708056	2.56687982036695E-4
50-51	1.91349222972809E-4	5.78714723137276E-4	0.0	0.04892472937619406	2.61355036255544E-4
52-53	2.05350385629356E-4	5.78714723137276E-4	0.0	0.05517858202945172	2.89357361568638E-4
54-55	2.05350385629356E-4	5.83381777356125E-4	0.0	0.062048485839597455	3.17359686881732E-4
56-57	2.05350385629356E-4	5.97382940012672E-4	0.0	0.06870370515567613	3.2669379531942997E-4
58-59	2.05350385629356E-4	6.0671704845037E-4	0.0	0.07454218998345623	3.45362012194826E-4
60-61	2.10017439848205E-4	6.16051156888068E-4	0.0	0.08040867713654942	3.45362012194826E-4
62-63	2.24018602504752E-4	6.25385265325766E-4	0.0	0.08583646119307081	3.50029066413675E-4
64-65	2.24018602504752E-4	7.09392241265048E-4	0.0	0.0914462603641273	3.59363174851373E-4
66-67	2.42686819380148E-4	7.14059295483897E-4	0.0	0.0973314157340959	3.92032554383316E-4
68-69	2.42686819380148E-4	7.37394566578142E-4	0.0	0.10331457924266031	4.06033717039863E-4
70-71	2.42686819380148E-4	7.4672867501584E-4	0.0	0.1089430466305922	4.10700771258712E-4
72-73	2.52020927817846E-4	7.56062783453538E-4	0.0	0.11295671325880234	4.667054218849E-4
74-75	2.52020927817846E-4	7.79398054547783E-4	0.0	0.11643366865184485	4.80706584541447E-4
76-77	2.52020927817846E-4	8.07400379860877E-4	0.0	0.11940658218925165	4.94707747197994E-4
78-79	2.52020927817846E-4	8.21401542517424E-4	0.0	0.12206213603977675	4.94707747197994E-4
80-81	2.52020927817846E-4	8.260685967362729E-4	0.0	0.12318689610651934	5.04041855635692E-4
82-83	2.52020927817846E-4	8.447368136116689E-4	0.0	0.12354625928137072	5.32044180948786E-4
84-85	2.52020927817846E-4	8.494038678305179E-4	0.0	0.12377027788387548	5.32044180948786E-4
86-87	2.52020927817846E-4	8.72739138924763E-4	0.0	0.12381694842606397	5.41378289386484E-4
88-89	2.61355036255544E-4	9.14742626894404E-4	0.0	0.12383561664293936	5.64713560480729E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15840	0.0	50.689167	1
GTATCAA	28525	0.0	37.63584	1
ATCAACG	35910	0.0	29.306986	3
TCAACGC	35910	0.0	29.283335	4
CAACGCA	36605	0.0	28.750952	5
TATCAAC	37335	0.0	28.39815	2
AACGCAG	37745	0.0	27.985022	6
GTGGTAT	7460	0.0	25.1078	1
TGGTATC	7270	0.0	24.582153	2
ACGCAGA	43410	0.0	24.26371	7
CGCAGAG	43755	0.0	24.052761	8
GCAGAGT	50255	0.0	20.856487	9
GTACATG	34040	0.0	20.129461	1
TACATGG	33360	0.0	20.101934	2
GAGTACT	29985	0.0	20.013403	12-13
ACATGGG	34060	0.0	19.37167	3
AGAGTAC	45860	0.0	19.216316	10-11
CAGAGTA	49270	0.0	18.605635	10-11
AGTACTT	32615	0.0	17.918835	12-13
GTACTTT	33420	0.0	17.892647	14-15
>>END_MODULE