FastQCFastQC Report
Fri 27 May 2016
SRR2140036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9975083
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170800.040.3359221
GTATCAA281000.032.470581
CTTATAC76550.027.8472841
TCAACGC337300.026.6924174
ATCAACG337350.026.6883283
CAACGCA345000.026.0720215
TATCAAC351550.025.7464642
AACGCAG354300.025.4361766
ACGCAGA401650.022.4054417
CGCAGAG405500.022.213918
TATACAC118650.021.333673
GAGTACT254000.019.83000612-13
GCAGAGT459850.019.5327519
TTATACA119000.019.4665132
AGAGTAC392650.018.54138610-11
GTACATG295400.018.40461
TACATGG289250.018.2015782
GTACTTT280350.017.89801214-15
CAGAGTA446850.017.61006510-11
TATGCCG61250.017.53686742-43
Produced by FastQC (version 0.11.5)