FastQCFastQC Report
Fri 27 May 2016
SRR2140035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10083119
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133550.050.252461
GTATCAA239700.037.636311
ATCAACG282850.031.0704733
TCAACGC293700.029.9373044
CAACGCA301450.029.153665
TATCAAC307850.028.8006672
AACGCAG306100.028.7248346
ACGCAGA310500.028.3177857
CGCAGAG360350.024.3764938
GTGGTAT64300.022.6142581
TGGTATC61450.022.332252
GCAGAGT420050.020.902039
GAGTACT257450.019.97565512-13
GTACATG276050.019.464861
AGAGTAC378750.019.34241310-11
TACATGG272050.018.8965642
CAGAGTA410450.018.61364210-11
ACATGGG266550.018.6074283
AGTACTT281100.017.7900212-13
GTACTTT286950.017.72760214-15
Produced by FastQC (version 0.11.5)