##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140035_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10083119
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78516558219734	34.0	31.0	34.0	31.0	34.0
2	32.939905697830206	34.0	31.0	34.0	31.0	34.0
3	33.60762746130438	34.0	33.0	34.0	31.0	37.0
4	36.32592871312934	37.0	37.0	37.0	35.0	37.0
5	36.282380779201354	37.0	37.0	37.0	35.0	37.0
6	36.31561236161152	37.0	37.0	37.0	35.0	37.0
7	36.32733611494618	37.0	37.0	37.0	35.0	37.0
8	36.59419947339707	37.0	37.0	37.0	35.0	39.0
9	37.931789855896774	39.0	38.0	39.0	35.0	39.0
10-11	37.91988565244544	39.0	38.0	39.0	35.0	39.0
12-13	38.06929056376306	39.0	38.0	39.0	35.0	40.0
14-15	39.315937756957936	41.0	39.0	41.0	36.0	41.0
16-17	39.29543308970171	41.0	39.0	41.0	36.0	41.0
18-19	39.31117102753622	41.0	39.0	41.0	36.0	41.0
20-21	39.24446390842006	41.0	39.0	41.0	36.0	41.0
22-23	39.22932807385551	40.0	39.0	41.0	36.0	41.0
24-25	39.13471632734421	40.0	39.0	41.0	36.0	41.0
26-27	38.99985254143234	40.0	39.0	41.0	35.0	41.0
28-29	38.88994856212783	40.0	39.0	41.0	35.0	41.0
30-31	38.77252686669688	40.0	38.0	41.0	35.0	41.0
32-33	38.68196651452342	40.0	38.0	41.0	35.0	41.0
34-35	38.556475622000384	40.0	38.0	41.0	34.5	41.0
36-37	38.7002899963131	40.0	38.0	41.0	35.0	41.0
38-39	38.75226548791627	40.0	38.0	41.0	35.0	41.0
40-41	38.72762069350019	40.0	38.0	41.0	35.0	41.0
42-43	38.68652519262667	40.0	38.0	41.0	35.0	41.0
44-45	38.61585296648374	40.0	38.0	41.0	35.0	41.0
46-47	38.54515127476746	40.0	38.0	41.0	34.0	41.0
48-49	38.45648609263198	40.0	38.0	41.0	34.0	41.0
50-51	38.3612511734697	40.0	38.0	41.0	34.0	41.0
52-53	38.24492483129656	40.0	37.5	41.0	34.0	41.0
54-55	38.106757012834095	40.0	37.0	41.0	33.5	41.0
56-57	37.956187119656455	40.0	37.0	41.0	33.0	41.0
58-59	37.76423811401307	39.5	36.5	41.0	33.0	41.0
60-61	37.541509975370275	39.0	36.0	41.0	33.0	41.0
62-63	37.333843780932426	39.0	35.5	40.0	33.0	41.0
64-65	37.08704079381148	38.5	35.0	40.0	32.0	41.0
66-67	36.79385293741148	38.0	35.0	40.0	32.0	41.0
68-69	36.44914790658491	37.0	35.0	40.0	31.5	41.0
70-71	36.156663743893034	37.0	35.0	39.0	31.0	41.0
72-73	35.81677955308403	36.0	34.5	39.0	31.0	40.0
74-75	35.38010220067494	36.0	34.0	38.5	31.0	40.0
76-77	34.55397105439462	35.0	33.5	37.0	29.5	39.0
78-79	34.771390525364296	35.0	34.0	37.0	30.5	39.0
80-81	34.58034878772463	35.0	34.0	37.0	31.0	39.0
82-83	34.30186194713559	35.0	34.0	36.0	30.0	38.0
84-85	34.03545040981659	35.0	34.0	36.0	30.0	37.0
86-87	33.81272564934946	35.0	34.0	35.5	30.0	37.0
88-89	33.62893599738727	35.0	33.0	35.0	30.0	36.5
90-91	33.44446997638205	35.0	33.0	35.0	30.0	36.0
92-93	33.31366989117728	34.5	33.0	35.0	29.5	36.0
94-95	33.23126738633333	34.0	33.0	35.0	29.0	35.5
96-97	33.21278122998122	34.0	33.0	35.0	29.5	35.0
98-99	33.19793000734262	34.0	33.0	35.0	29.5	35.0
100-101	32.017155817571044	33.0	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	1.0
19	7.0
20	38.0
21	219.0
22	1064.0
23	3560.0
24	9484.0
25	19828.0
26	35541.0
27	56994.0
28	83073.0
29	112552.0
30	146537.0
31	186049.0
32	237601.0
33	311101.0
34	438103.0
35	642000.0
36	1117704.0
37	2305669.0
38	3042736.0
39	1263881.0
40	69375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78702259929255	0.0024199430281937246	26.614602848669655	30.595954609009606
2	14.011149934986813	24.66409853836869	38.02739777655895	23.297353750085545
3	15.6399271812404	24.54403975913795	33.71972513258798	26.096307927033667
4	12.660719166575094	24.044868669566036	37.346104120987995	25.948308042870877
5	17.35918565657822	36.400232190105505	32.2613731448321	13.979209008484181
6	27.879437269193375	37.63085736591744	20.331135732247848	14.158569632641338
7	26.00984913888218	33.06174728742061	22.316724543952933	18.61167902974428
8	23.997442853974903	32.52780192154886	22.074148507267	21.400606717209243
9	23.292110912263635	20.987185990823264	23.990913513248152	31.729789583664953
10-11	24.420252653107553	26.43278171863837	28.646445670695662	20.50051995755841
12-13	24.68315968412739	24.345922646626434	28.648347824638126	22.322569844608047
14-15	22.073623423727028	26.991693279834923	26.74986643414422	24.184816862293825
16-17	21.287393739862924	29.23391243789818	27.724466574459004	21.754227247779887
18-19	21.326569219928565	28.276261849636747	29.512105138144314	20.885063792290378
20-21	22.045542544552337	27.54844215088982	29.43708401717468	20.96893128738316
22-23	21.8609744821559	27.286518035832124	29.271675120640094	21.58083236137188
24-25	21.593849998087684	27.548741312922882	29.42172764749212	21.435681041497315
26-27	21.616530827077522	27.77722505961909	29.188806007642604	21.41743810566078
28-29	21.580153072071646	27.661277056804177	29.26636194884938	21.492207922274794
30-31	21.77084493405572	27.517667910579828	29.304030436174532	21.40745671918992
32-33	21.59149395532356	27.8234200786194	29.125747987446704	21.45933797861034
34-35	21.796306364066975	27.651502937636618	29.054739649533268	21.497451048763143
36-37	21.671281358449768	27.722277161416166	29.133342235888847	21.47309924424522
38-39	21.527596053591232	27.950461299728996	29.11364776947249	21.408294877207286
40-41	21.882914935537336	27.639635512762844	28.889604299378423	21.5878452523214
42-43	21.68395936996125	27.7577547072505	28.87733833700591	21.68094758578234
44-45	21.82888657563644	27.99454192466529	28.534444228546967	21.642127271151303
46-47	21.919177339257303	27.866445184376648	28.276030610728675	21.93834686563737
48-49	21.783191549210255	28.030156518614742	28.325443799659478	21.86120813251553
50-51	21.856626162914576	28.259916037808413	28.059316349988094	21.824141449288913
52-53	22.07934968493963	27.978865629977072	27.969116247941578	21.97266843714172
54-55	21.94962164339245	27.968801235957443	28.107348870642713	21.974228250007393
56-57	21.93707832675927	28.226222556543696	27.961890077279328	21.874809039417705
58-59	22.010431975055198	28.02955256454527	28.016032846301837	21.943982614097695
60-61	22.020611088621454	28.07464308366535	28.074659472889763	21.83008635482343
62-63	21.984026448518648	28.27410985597182	27.918527323616217	21.82333637189332
64-65	22.041888282488088	28.030763106755067	27.87448507734861	22.05286353340824
66-67	21.94729415272994	28.094503724645342	27.898627946056337	22.059574176568383
68-69	22.008987531511195	28.185176446324256	27.879442021261635	21.92639400090291
70-71	22.036738307591783	27.8588735610763	27.918366788656563	22.18602134267535
72-73	21.977205863156744	27.968901361384873	27.947527114011972	22.10636566144641
74-75	21.888652499017823	28.15387504902171	27.883072712968616	22.07439973899185
76-77	22.099095629751645	27.922829633210966	27.75518687070279	22.222887866334602
78-79	21.900582474850474	28.012470300571408	27.92846212044784	22.158485104130285
80-81	21.99191820140995	28.06675254222462	27.832440012831743	22.108889243533685
82-83	22.070791810525154	27.900127053740114	27.92043826325151	22.10864287248322
84-85	22.091982603044112	27.92273410580191	27.956005338528843	22.029277952625133
86-87	21.978679334368202	28.01361188111555	27.901129640779278	22.106579143736965
88-89	22.053378390359306	27.881694588386786	27.897334764196096	22.16759225705781
90-91	22.05637303599273	27.85729290785512	27.98702961823945	22.099304437912696
92-93	22.002475169865573	27.93751905957147	27.972556911808233	22.087448858754723
94-95	22.015215643786163	27.835620640622405	27.97627793573968	22.172885779851754
96-97	21.878744270418686	27.951940918105077	28.135627878085888	22.033686933390342
98-99	22.281928821156708	28.87589931146578	28.044756722092295	20.797415145285214
100-101	22.6634442335684	30.961476306706707	24.998130618152235	21.37694884157266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2015.0
1	1711.0
2	2182.5
3	3137.5
4	4283.0
5	6600.5
6	8567.5
7	10679.5
8	12676.0
9	13271.5
10	12343.5
11	11266.0
12	12463.0
13	16723.0
14	27681.5
15	43791.0
16	57289.0
17	64359.5
18	64554.5
19	60317.5
20	54437.0
21	48525.5
22	45526.5
23	47842.5
24	55514.5
25	68150.5
26	85635.5
27	105745.0
28	127793.5
29	151836.5
30	175452.0
31	198942.5
32	224266.0
33	248638.5
34	271594.0
35	292379.5
36	315096.5
37	336957.5
38	353872.0
39	368425.0
40	380508.0
41	391987.5
42	399226.5
43	405012.0
44	410812.0
45	415441.0
46	418759.0
47	418274.5
48	413228.0
49	403506.5
50	392676.0
51	376728.5
52	355536.5
53	333767.0
54	311157.5
55	287386.0
56	262905.0
57	236893.5
58	209207.5
59	180757.0
60	151537.5
61	124044.0
62	98533.5
63	76170.0
64	57003.0
65	42212.5
66	31818.5
67	24363.5
68	18641.5
69	13668.5
70	9806.5
71	7092.5
72	5087.0
73	3509.5
74	2395.5
75	1608.0
76	1054.0
77	644.5
78	390.0
79	246.0
80	156.5
81	114.5
82	91.5
83	68.5
84	52.0
85	38.5
86	27.0
87	18.0
88	10.5
89	7.0
90	5.5
91	4.5
92	3.5
93	2.0
94	0.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002350463185052165
2	0.005444743833728433
3	2.9752698544964117E-5
4	2.9752698544964117E-5
5	1.983513236330941E-5
6	2.9752698544964117E-5
7	3.967026472661882E-5
8	2.9752698544964117E-5
9	1.3884592654316586E-4
10-11	8.380343423498224E-4
12-13	3.4711481635791464E-5
14-15	7.735701621690669E-4
16-17	8.033228607140311E-4
18-19	3.967026472661882E-5
20-21	5.256475854278985E-4
22-23	0.0016812112933955225
24-25	0.00831824607524615
26-27	0.056092579500005485
28-29	0.31666533289302257
30-31	0.3110958754749803
32-33	0.310905086775626
34-35	0.1545301044948749
36-37	0.044618178372804844
38-39	0.035177539065775
40-41	0.03193508271626942
42-43	0.026319428190807806
44-45	0.014139741514845856
46-47	0.008542661788767067
48-49	0.0058835568849816364
50-51	0.00243234564832041
52-53	0.0018554760186616356
54-55	0.0017350853531194993
56-57	7.638364946661083E-4
58-59	0.0034909360880071494
60-61	0.0027423883427299288
62-63	5.339792463736204E-4
64-65	0.001392694446500503
66-67	0.00768399516708604
68-69	0.007149123718465927
70-71	0.009396983756267884
72-73	0.009504512458472381
74-75	0.0074843346783282265
76-77	0.004028838991665925
78-79	4.435158077608525E-4
80-81	6.094382992417333E-4
82-83	5.97740107672918E-4
84-85	2.0744903099337335E-4
86-87	0.002029722791689365
88-89	0.001304891852249031
90-91	0.0024542471094124232
92-93	0.009950247479527254
94-95	8.470576215508139E-5
96-97	4.65332991291426E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	187.0
20-21	662.0
22-23	1289.0
24-25	2913.0
26-27	4704.0
28-29	8321.0
30-31	16758.0
32-33	23145.0
34-35	34922.0
36-37	47052.0
38-39	54406.0
40-41	58260.0
42-43	71185.0
44-45	85975.0
46-47	85087.0
48-49	74403.0
50-51	66277.0
52-53	65024.0
54-55	69472.0
56-57	71376.0
58-59	71350.0
60-61	70099.0
62-63	70419.0
64-65	75878.0
66-67	79715.0
68-69	80291.0
70-71	78770.0
72-73	77649.0
74-75	79104.0
76-77	81900.0
78-79	86219.0
80-81	87629.0
82-83	85910.0
84-85	89307.0
86-87	94268.0
88-89	101514.0
90-91	110077.0
92-93	115573.0
94-95	136782.0
96-97	253569.0
98-99	1025708.0
100-101	6289970.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.12510563046484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0621790629884	74.92365221353275
2	7.528269631927036	12.666329560009654
3	1.9124692517818946	4.82660033463504
4	0.7027598224201884	2.3647897717578026
5	0.31920223167514356	1.3426460728575784
6	0.16117574623378125	0.8135356006191508
7	0.08825710022120202	0.5197246515123001
8	0.05394201451849136	0.3630302135430523
9	0.04287917486245093	0.3246493603185771
>10	0.12585171851227225	1.6264721525027868
>50	0.002545597176910414	0.14216675588788405
>100	4.4894123531453143E-4	0.07484839855374315
>500	1.970644687962257E-5	0.01155491426968527
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9752698544964114E-5	0.0	9.917566181654705E-6	0.0
3	0.0	2.9752698544964114E-5	0.0	9.917566181654705E-6	0.0
4	0.0	2.9752698544964114E-5	0.0	9.917566181654705E-6	0.0
5	0.0	3.967026472661882E-5	0.0	9.917566181654705E-6	0.0
6	2.9752698544964114E-5	3.967026472661882E-5	0.0	3.967026472661882E-5	0.0
7	2.9752698544964114E-5	3.967026472661882E-5	0.0	4.958783090827352E-5	0.0
8	4.958783090827352E-5	5.950539708992823E-5	0.0	6.942296327158293E-5	0.0
9	5.950539708992823E-5	1.4876349272482055E-4	0.0	8.925809563489234E-5	0.0
10-11	6.446418018075558E-5	1.537222758156479E-4	0.0	8.925809563489234E-5	9.917566181654705E-6
12-13	7.934052945323764E-5	1.5868105890647527E-4	0.0	8.925809563489234E-5	9.917566181654705E-6
14-15	8.925809563489234E-5	2.380215883597129E-4	0.0	1.041344449073744E-4	9.917566181654705E-6
16-17	1.4876349272482055E-4	2.67774286904677E-4	0.0	1.2396957727068382E-4	9.917566181654705E-6
18-19	1.537222758156479E-4	2.67774286904677E-4	0.0	1.4876349272482058E-4	9.917566181654705E-6
20-21	1.5868105890647527E-4	2.776918530863317E-4	0.0	1.5868105890647527E-4	9.917566181654705E-6
22-23	1.6363984199730262E-4	3.2727968399460524E-4	0.0	1.785161912697847E-4	9.917566181654705E-6
24-25	1.6859862508812997E-4	3.4215603326708725E-4	0.0	2.082688898147488E-4	9.917566181654705E-6
26-27	1.6859862508812997E-4	3.4711481635791463E-4	0.0	3.024857685404685E-4	9.917566181654705E-6
28-29	1.6859862508812997E-4	3.52073599448742E-4	0.0	6.446418018075557E-4	9.917566181654705E-6
30-31	1.6859862508812997E-4	3.8182629799370614E-4	0.0	0.0015818518059739252	9.917566181654705E-6
32-33	1.6859862508812997E-4	3.9670264726618815E-4	0.0	0.003327343453945153	9.917566181654705E-6
34-35	1.785161912697847E-4	4.6116682744694375E-4	0.0	0.0055290431462724976	9.917566181654705E-6
36-37	1.785161912697847E-4	5.157134414460446E-4	0.0	0.00938201760784535	9.917566181654705E-6
38-39	1.8843375745143938E-4	5.35548573809354E-4	0.0	0.014206913555220364	9.917566181654705E-6
40-41	1.9835132363309408E-4	5.603424892634907E-4	0.0	0.019413635800589087	9.917566181654705E-6
42-43	2.082688898147488E-4	6.297654525350737E-4	0.0	0.024640193178321113	9.917566181654705E-6
44-45	2.380215883597129E-4	6.942296327158293E-4	0.0	0.03088330108967275	9.917566181654705E-6
46-47	2.380215883597129E-4	6.942296327158293E-4	0.0	0.0376917102733787	9.917566181654705E-6
48-49	2.380215883597129E-4	7.091059819883113E-4	0.0	0.044926574802895816	9.917566181654705E-6
50-51	2.578567207230223E-4	7.983640776232036E-4	0.0	0.05123910567751903	9.917566181654705E-6
52-53	2.67774286904677E-4	8.132404268956857E-4	0.0	0.057640894647777136	9.917566181654705E-6
54-55	2.876094192679864E-4	8.231579930773404E-4	0.0	0.06441955113293812	9.917566181654705E-6
56-57	3.0744455163129585E-4	8.529106916223046E-4	0.0	0.06973040782321423	9.917566181654705E-6
58-59	3.0744455163129585E-4	8.677870408947865E-4	0.0	0.07481811927440309	9.917566181654705E-6
60-61	3.1736211781295055E-4	9.024985225305781E-4	0.0	0.08032236850522145	9.917566181654705E-6
62-63	3.4711481635791463E-4	9.024985225305781E-4	0.0	0.08517701715114143	9.917566181654705E-6
64-65	3.52073599448742E-4	0.0010016741843471252	0.0	0.08991761378597238	9.917566181654705E-6
66-67	3.6199116563039675E-4	0.0010115917505287798	0.0	0.09495573740625296	9.917566181654705E-6
68-69	3.6694994872122407E-4	0.0010115917505287798	0.0	0.10004344885744183	9.917566181654705E-6
70-71	3.6694994872122407E-4	0.001016550533619607	0.0	0.10437742527882493	9.917566181654705E-6
72-73	3.8678508108453346E-4	0.0010264680998012619	0.0	0.10798741936894725	9.917566181654705E-6
74-75	3.8678508108453346E-4	0.0010661383645278809	0.0	0.11124038107652999	9.917566181654705E-6
76-77	3.8678508108453346E-4	0.0011157261954361543	0.0	0.11415614553393648	9.917566181654705E-6
78-79	4.0662021344784285E-4	0.0011306025447086364	0.0	0.11675454787353001	9.917566181654705E-6
80-81	4.264553458111523E-4	0.0011306025447086364	0.0	0.11781572745496706	9.917566181654705E-6
82-83	4.3141412890197967E-4	0.0011306025447086364	0.0	0.11817771862059745	9.917566181654705E-6
84-85	4.36372911992807E-4	0.0011504376770719456	0.0	0.11839590507659387	9.917566181654705E-6
86-87	4.413316950836343E-4	0.0011603552432536005	0.0	0.11846532803986544	9.917566181654705E-6
88-89	4.462904781744617E-4	0.0012198606403435288	0.0	0.11848516317222875	9.917566181654705E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13355	0.0	50.25246	1
GTATCAA	23970	0.0	37.63631	1
ATCAACG	28285	0.0	31.070473	3
TCAACGC	29370	0.0	29.937304	4
CAACGCA	30145	0.0	29.15366	5
TATCAAC	30785	0.0	28.800667	2
AACGCAG	30610	0.0	28.724834	6
ACGCAGA	31050	0.0	28.317785	7
CGCAGAG	36035	0.0	24.376493	8
GTGGTAT	6430	0.0	22.614258	1
TGGTATC	6145	0.0	22.33225	2
GCAGAGT	42005	0.0	20.90203	9
GAGTACT	25745	0.0	19.975655	12-13
GTACATG	27605	0.0	19.46486	1
AGAGTAC	37875	0.0	19.342413	10-11
TACATGG	27205	0.0	18.896564	2
CAGAGTA	41045	0.0	18.613642	10-11
ACATGGG	26655	0.0	18.607428	3
AGTACTT	28110	0.0	17.79002	12-13
GTACTTT	28695	0.0	17.727602	14-15
>>END_MODULE