FastQCFastQC Report
Fri 27 May 2016
SRR2140034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10172137
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100000.045.2833981
GTATCAA177250.033.1008031
ATCAACG218400.029.5735553
TCAACGC220750.029.3157654
CAACGCA224800.028.6755965
TATCAAC237500.027.6016482
AACGCAG236000.027.4033666
ACGCAGA272100.023.690487
TACATGG309700.023.213132
CGCAGAG284100.022.660178
ACATGGG310400.022.5793953
GTACATG292900.022.1947171
TCGCAGA29750.020.313891
CATGGGG225750.020.1148624
GCAGAGT344050.018.784879
GAGTACT176700.018.52537712-13
AGAGTAC297300.017.72979510-11
CAGAGTA329500.017.4300410-11
CGTCGAA4253.5519275E-516.88302494
ATGGGGG153000.016.046565
Produced by FastQC (version 0.11.5)