##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140034_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10172137
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.11764705882353	18.0	18.0	18.0	18.0	31.0
2	29.55257769335981	30.0	27.0	32.0	27.0	33.0
3	31.654796332373422	32.0	32.0	33.0	28.0	33.0
4	32.22547209106602	33.0	33.0	33.0	32.0	33.0
5	32.812471361720746	33.0	33.0	34.0	32.0	34.0
6	36.87755591573334	38.0	37.0	38.0	35.0	38.0
7	37.162695803251566	38.0	38.0	38.0	36.0	38.0
8	37.203727987540866	38.0	38.0	38.0	36.0	38.0
9	37.32810008359109	38.0	38.0	38.0	37.0	38.0
10-11	37.380325982632755	38.0	38.0	38.0	37.0	38.0
12-13	37.3942312711675	38.0	38.0	38.0	37.0	38.0
14-15	37.3993744382326	38.0	38.0	38.0	37.0	38.0
16-17	37.35934287947557	38.0	38.0	38.0	37.0	38.0
18-19	37.34796464105822	38.0	38.0	38.0	37.0	38.0
20-21	37.39841647208888	38.0	38.0	38.0	37.0	38.0
22-23	37.41665709720153	38.0	38.0	38.0	37.0	38.0
24-25	37.422433525303845	38.0	38.0	38.0	37.0	38.0
26-27	37.328263612675535	38.0	38.0	38.0	37.0	38.0
28-29	37.240081398395375	38.0	38.0	38.0	37.0	38.0
30-31	37.323155083235534	38.0	38.0	38.0	37.0	38.0
32-33	37.34461502572319	38.0	38.0	38.0	37.0	38.0
34-35	37.304001494663844	38.0	38.0	38.0	37.0	38.0
36-37	37.31114962944115	38.0	38.0	38.0	37.0	38.0
38-39	37.31304464825621	38.0	38.0	38.0	37.0	38.0
40-41	37.273347385490766	38.0	38.0	38.0	37.0	38.0
42-43	37.27441721259819	38.0	38.0	38.0	37.0	38.0
44-45	37.21435163328104	38.0	38.0	38.0	37.0	38.0
46-47	37.19544947015021	38.0	38.0	38.0	37.0	38.0
48-49	37.174631743202454	38.0	38.0	38.0	37.0	38.0
50-51	37.16728013744472	38.0	38.0	38.0	36.5	38.0
52-53	37.15005632846947	38.0	38.0	38.0	36.0	38.0
54-55	37.10468382779027	38.0	38.0	38.0	36.0	38.0
56-57	37.105857826179516	38.0	38.0	38.0	36.0	38.0
58-59	37.11721939211753	38.0	38.0	38.0	36.0	38.0
60-61	37.128261197172506	38.0	38.0	38.0	36.0	38.0
62-63	37.05888059802976	38.0	38.0	38.0	36.0	38.0
64-65	37.090839913946894	38.0	38.0	38.0	36.0	38.0
66-67	37.108175550769026	38.0	38.0	38.0	36.0	38.0
68-69	37.08009867761771	38.0	38.0	38.0	36.0	38.0
70-71	37.035074067026216	38.0	38.0	38.0	36.0	38.0
72-73	37.045102846287534	38.0	38.0	38.0	36.0	38.0
74-75	37.06231479914053	38.0	38.0	38.0	36.0	38.0
76-77	36.99894315235748	38.0	38.0	38.0	36.0	38.0
78-79	37.02337732069836	38.0	38.0	38.0	36.0	38.0
80-81	37.00035398491714	38.0	38.0	38.0	36.0	38.0
82-83	36.95344074977663	38.0	38.0	38.0	36.0	38.0
84-85	36.978003327483584	38.0	38.0	38.0	36.0	38.0
86-87	36.99996978915335	38.0	38.0	38.0	36.0	38.0
88-89	36.913247627370154	38.0	38.0	38.0	35.5	38.0
90-91	36.869614287209544	38.0	38.0	38.0	35.0	38.0
92-93	36.92258264721566	38.0	38.0	38.0	35.0	38.0
94-95	36.90587319063117	38.0	38.0	38.0	35.0	38.0
96-97	36.854338822833896	38.0	38.0	38.0	35.0	38.0
98-99	36.8752076182152	38.0	38.0	38.0	35.0	38.0
100	35.306123445091636	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	7.0
19	40.0
20	119.0
21	308.0
22	815.0
23	2038.0
24	4539.0
25	8784.0
26	15656.0
27	25045.0
28	37829.0
29	55431.0
30	77516.0
31	104298.0
32	139090.0
33	192194.0
34	292367.0
35	562419.0
36	1955512.0
37	6698127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.81063536600028	13.890385078376353	22.880147996433788	24.418831559189577
2	15.251294885743235	21.19027543190794	39.8089663200432	23.749463362305626
3	18.61778896607468	25.87374707989088	29.89673654611612	25.611727407918316
4	12.607514334261491	17.31404979801678	38.13638084916712	31.942055018554615
5	13.39617844714343	37.48816099590577	35.59594553065184	13.51971502629896
6	30.873621021241316	37.30942377644129	18.380845431199088	13.436109771118307
7	27.06171933132189	31.98118112737265	23.356939338854236	17.600160202451224
8	25.371591043258658	35.82422257977847	21.09496755696468	17.709218819998195
9	26.341780493125487	15.602277082976762	20.66345547646478	37.39248694743297
10-11	24.888860619946428	25.991534522195288	28.783017767063107	20.33658709079518
12-13	25.58206304142384	23.65306326487738	28.83486036414964	21.93001332954914
14-15	22.664927733474293	24.799970743610707	26.07916605920663	26.455935463708364
16-17	22.550418854956437	27.855887115952132	26.734937801171966	22.858756227919464
18-19	22.6066902166182	27.202445267892085	28.27108502372707	21.919779491762643
20-21	23.2554328340572	26.48600426681379	28.025609140706255	22.23295375842276
22-23	23.16986656101395	26.376197072462887	27.894494425213512	22.559441941309654
24-25	22.812427906054854	26.543962577438695	27.955114988751806	22.688494527754642
26-27	22.808220834814787	26.662217724553912	27.892064744398542	22.637496696232763
28-29	22.78351141199356	26.597946673398027	27.927639144632195	22.690902769976226
30-31	22.901241789195073	26.5661367699492	27.834125670416178	22.69849577043955
32-33	22.68749780242833	26.771776108040328	27.869110063475745	22.671616026055595
34-35	22.879212782475662	26.695500488151712	27.896219446178787	22.529067283193832
36-37	22.888805455844103	26.64480581506418	27.793160123146375	22.67322860594533
38-39	22.786232427268196	26.709498994783388	27.91933528549822	22.584933292450195
40-41	22.875955443861393	26.529223565391412	27.91842394288019	22.676397047867006
42-43	22.74752051349337	26.697033008139424	27.72654548850999	22.828900989857214
44-45	22.841950499252732	26.7538033760263	27.6379480835585	22.766298041162468
46-47	22.959454573037046	26.75275508571262	27.4789485059258	22.808841835324532
48-49	22.849060919345618	26.855333388406557	27.407003319403728	22.8886023728441
50-51	22.806515029318458	26.953966199811685	27.338437815216444	22.901080955653416
52-53	22.960119302699244	26.818836715211997	27.288699979512305	22.932344002576453
54-55	22.94508204014499	26.794352818407674	27.24474431670934	23.015820824737993
56-57	22.913482288063015	26.846758774383346	27.355906805717055	22.883852131836584
58-59	22.916060985575836	26.859406299164117	27.298657837321024	22.925874877939027
60-61	22.98670280146116	26.85918141714181	27.344651832103413	22.80946394929362
62-63	23.00483041825779	26.838700901895407	27.276192681640925	22.880275998205875
64-65	22.97901797168108	26.856484365472248	27.238364783193543	22.92613287965313
66-67	22.954556593522973	26.813452768533587	27.22705824658632	23.004932391357123
68-69	22.90236656495817	26.845196903970525	27.28925228728239	22.963184243788916
70-71	22.97532115304634	26.692583458787556	27.260314094415772	23.07178129375033
72-73	23.00483708514845	26.752592183805685	27.187876555739578	23.05469417530629
74-75	22.871644158166255	26.913568446992723	27.22752092204056	22.987266472800467
76-77	23.103996577547345	26.790734603721493	27.121916114259403	22.983352704471756
78-79	22.945167640957763	26.771077495246832	27.105285065272138	23.178469798523267
80-81	22.999208744943473	26.909417047006478	27.038840678539817	23.05253352951023
82-83	23.036540966561216	26.785919299530388	27.17704015160118	23.000499582307217
84-85	23.063127375414886	26.856633583249938	27.066918255904632	23.013320785430547
86-87	22.946653681420074	26.865074909071712	27.180045570793222	23.008225838715
88-89	23.025375122344396	26.79396607261351	27.083035629975754	23.09762317506633
90-91	23.125911019551662	26.83196920013885	27.05531487730625	22.986804903003232
92-93	23.096518610436405	26.85248616637893	27.04599161581932	23.005003607365346
94-95	22.990737496681806	26.825222571675784	27.117884276011374	23.066155655631036
96-97	23.162623933713526	26.93012877791095	27.070156677666112	22.837090610709414
98-99	24.256974662293246	28.892190872951574	27.21683734844007	19.633997116315108
100	31.267422568582592	39.998262213838586	28.73431521757882	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1421.0
1	1843.0
2	3271.5
3	4454.0
4	5223.0
5	6521.5
6	7836.0
7	10311.0
8	12354.5
9	12639.5
10	12312.5
11	12027.5
12	12963.5
13	15106.5
14	19650.5
15	25065.5
16	27883.0
17	28161.0
18	26923.0
19	24892.0
20	23521.5
21	24094.5
22	27347.0
23	33737.0
24	43759.0
25	57187.0
26	73108.5
27	91017.5
28	110298.5
29	132206.5
30	152663.5
31	171647.0
32	192452.0
33	212697.0
34	230802.0
35	250554.0
36	270436.5
37	290788.5
38	313022.0
39	327729.0
40	340057.0
41	356156.5
42	369760.0
43	382925.5
44	401174.0
45	418834.0
46	430735.5
47	440339.0
48	444264.0
49	443312.5
50	443460.5
51	441792.5
52	435075.0
53	425735.0
54	411871.0
55	391153.5
56	366188.0
57	335975.0
58	300840.5
59	259844.0
60	217931.5
61	180221.0
62	146423.5
63	115288.5
64	87375.0
65	65566.5
66	49339.0
67	37625.5
68	28148.5
69	20344.0
70	14726.0
71	10470.0
72	7209.5
73	4751.0
74	3135.0
75	2095.5
76	1398.0
77	887.0
78	555.0
79	347.0
80	223.5
81	146.0
82	99.5
83	70.0
84	53.0
85	37.0
86	25.5
87	17.5
88	9.5
89	5.0
90	4.0
91	2.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.9323103886626775E-5
3	0.0
4	4.915387985828347E-5
5	0.00246752476888583
6	2.5560017526307405E-4
7	7.864620777325355E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.916320053812072E-6
28-29	0.0010427463025790068
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.5295162996473342E-5
44-45	1.0286552492791698E-5
46-47	2.5976664123551248E-5
48-49	7.347756068774157E-5
50-51	4.506148400932274E-4
52-53	2.3007804996546155E-4
54-55	3.347420213429353E-4
56-57	5.397184239916724E-4
58-59	4.295624669464073E-4
60-61	7.79022352544006E-5
62-63	4.159290951057848E-4
64-65	9.079951755946333E-5
66-67	1.375759541078718E-4
68-69	5.619969222267524E-4
70-71	9.372446740485619E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	9.53930752233052E-4
90-91	0.0
92-93	0.0
94-95	8.009710438955493E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	102.0
20-21	255.0
22-23	461.0
24-25	739.0
26-27	3008.0
28-29	10649.0
30-31	25126.0
32-33	30072.0
34-35	41703.0
36-37	70509.0
38-39	84686.0
40-41	78129.0
42-43	82236.0
44-45	95436.0
46-47	98162.0
48-49	96572.0
50-51	89017.0
52-53	82846.0
54-55	88111.0
56-57	92210.0
58-59	92878.0
60-61	92063.0
62-63	85285.0
64-65	86721.0
66-67	91668.0
68-69	93986.0
70-71	93749.0
72-73	85433.0
74-75	84109.0
76-77	87025.0
78-79	91407.0
80-81	91567.0
82-83	84347.0
84-85	80384.0
86-87	82400.0
88-89	87887.0
90-91	89535.0
92-93	87977.0
94-95	99384.0
96-97	309667.0
98-99	2351462.0
100-101	4753174.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.94485562723276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.67656026355363	73.55264488580062
2	7.460540574005329	12.376269216239685
3	2.0452852709712555	5.089376745516491
4	0.8124589344075351	2.6955715606995345
5	0.3935206712777045	1.632025763273046
6	0.20499105832433592	1.0201772242551415
7	0.12591073145644388	0.7310553209801808
8	0.07329521484151826	0.48635688105573904
9	0.05103578595574895	0.38098403081297555
>10	0.15379139396994176	1.8582637440196295
>50	0.0022650239334263363	0.11961798603491573
>100	3.239651804646938E-4	0.04081065560743418
>500	2.111212264939731E-5	0.016845985704634203
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9661551943313388E-5	0.0
2	0.0	4.915387985828347E-5	0.0	1.9661551943313388E-5	0.0
3	0.0	4.915387985828347E-5	0.0	1.9661551943313388E-5	0.0
4	0.0	1.0813853568822362E-4	0.0	2.9492327914970078E-5	0.0
5	0.0	1.0813853568822362E-4	0.0	2.9492327914970078E-5	0.0
6	2.457693992914173E-4	1.0813853568822362E-4	0.0	3.9323103886626775E-5	0.0
7	2.457693992914173E-4	1.0813853568822362E-4	0.0	3.9323103886626775E-5	0.0
8	2.457693992914173E-4	1.376308636031937E-4	0.0	4.915387985828347E-5	9.830775971656694E-6
9	2.457693992914173E-4	2.55600175263074E-4	0.0	8.847698374491024E-5	9.830775971656694E-6
10-11	2.605155632489024E-4	2.55600175263074E-4	0.0	1.3271547561736536E-4	9.830775971656694E-6
12-13	2.752617272063874E-4	2.55600175263074E-4	0.0	1.572924155465071E-4	1.9661551943313388E-5
14-15	2.850925031780441E-4	2.998386671355292E-4	0.0	1.572924155465071E-4	4.4238491872455125E-5
16-17	5.898465582994016E-4	3.244156070646709E-4	0.0	1.8678474346147718E-4	4.915387985828347E-5
18-19	5.898465582994016E-4	3.391617710221559E-4	0.0	2.457693992914173E-4	5.406926784411181E-5
20-21	5.947619462852299E-4	3.637387109512976E-4	0.0	2.9983866713552914E-4	6.39000438157685E-5
22-23	5.996773342710583E-4	3.981464268520961E-4	0.0	3.391617710221559E-4	8.356159575908189E-5
24-25	6.045927222568866E-4	5.013695745544913E-4	0.0	3.588233229654693E-4	1.0322314770239529E-4
26-27	6.09508110242715E-4	5.308619024694615E-4	0.0	4.0797720282375276E-4	1.0813853568822362E-4
28-29	6.09508110242715E-4	5.357772904552898E-4	0.0	5.996773342710583E-4	1.0813853568822362E-4
30-31	6.09508110242715E-4	6.488312141293417E-4	0.0	0.0014795317837343323	1.0813853568822362E-4
32-33	6.242542742002E-4	6.488312141293417E-4	0.0	0.003136017534958485	1.0813853568822362E-4
34-35	6.684927660726551E-4	7.520543618317369E-4	0.0	0.005077595789360682	1.0813853568822362E-4
36-37	6.684927660726551E-4	8.454467335624757E-4	0.0	0.008311921084035735	1.0813853568822362E-4
38-39	6.684927660726551E-4	8.65108285505789E-4	0.0	0.01313883208611917	1.0813853568822362E-4
40-41	6.783235420443119E-4	9.388391052932142E-4	0.0	0.01837372029102636	1.0813853568822362E-4
42-43	6.783235420443119E-4	9.585006572365275E-4	0.0	0.02318588512915231	1.0813853568822362E-4
44-45	6.979850939876252E-4	0.0010469776409814378	0.0	0.0284895887658611	1.0813853568822362E-4
46-47	7.176466459309386E-4	0.001120708460768863	0.0	0.034171777277478665	1.1305392367405196E-4
48-49	7.274774219025954E-4	0.0011256238487546912	0.0	0.040340589199693244	1.27800087631537E-4
50-51	8.35615957590819E-4	0.001346816308116967	0.0	0.04644058569010622	1.27800087631537E-4
52-53	8.749390614774457E-4	0.0013664778600602804	0.0	0.05273719769995233	1.3271547561736536E-4
54-55	8.749390614774457E-4	0.001391054799989422	0.0	0.05821785530415094	1.376308636031937E-4
56-57	8.847698374491023E-4	0.0014352932918618771	0.0	0.06398852079951342	1.376308636031937E-4
58-59	8.847698374491023E-4	0.0014352932918618771	0.0	0.0695871477153719	1.376308636031937E-4
60-61	9.63416045222356E-4	0.0014549548438051907	0.0	0.0753774747626777	1.376308636031937E-4
62-63	0.0010322314770239529	0.0014844471717201607	0.0	0.08055829369974077	1.474616395748504E-4
64-65	0.0010371468650097813	0.001631908811295011	0.0	0.08544910474563996	1.474616395748504E-4
66-67	0.001076469968896408	0.0016417395872666677	0.0	0.0908806084699803	1.474616395748504E-4
68-69	0.0010813853568822362	0.0017056396310824364	0.0	0.09637109685015056	1.474616395748504E-4
70-71	0.0011010469088255496	0.001730216571011578	0.0	0.10150767729534119	1.6712319151816377E-4
72-73	0.0012239316084712583	0.0017400473469832347	0.0	0.10551863389177712	1.7695396748982047E-4
74-75	0.0012288469964570866	0.0017646242869123765	0.0	0.10868905914263641	1.7695396748982047E-4
76-77	0.0012878316522870268	0.001858016658643115	0.0	0.11176609202176493	1.7695396748982047E-4
78-79	0.0013271547561736535	0.0019022551505155703	0.0	0.11393869351150107	1.7695396748982047E-4
80-81	0.0013271547561736535	0.0019120859264872268	0.0	0.11462684782951704	1.8678474346147718E-4
82-83	0.0013369855321453102	0.0019170013144730552	0.0	0.11516262511997233	2.0644629540479055E-4
84-85	0.0013517316961027953	0.001926832090444712	0.0	0.11539364835530627	2.0644629540479055E-4
86-87	0.0013615624720744522	0.0019661551943313386	0.0	0.11549195611502283	2.0644629540479055E-4
88	0.0013959701879752504	0.0021332783858495023	0.0	0.11550178689099448	2.0644629540479055E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10000	0.0	45.283398	1
GTATCAA	17725	0.0	33.100803	1
ATCAACG	21840	0.0	29.573555	3
TCAACGC	22075	0.0	29.315765	4
CAACGCA	22480	0.0	28.675596	5
TATCAAC	23750	0.0	27.601648	2
AACGCAG	23600	0.0	27.403366	6
ACGCAGA	27210	0.0	23.69048	7
TACATGG	30970	0.0	23.21313	2
CGCAGAG	28410	0.0	22.66017	8
ACATGGG	31040	0.0	22.579395	3
GTACATG	29290	0.0	22.194717	1
TCGCAGA	2975	0.0	20.31389	1
CATGGGG	22575	0.0	20.114862	4
GCAGAGT	34405	0.0	18.78487	9
GAGTACT	17670	0.0	18.525377	12-13
AGAGTAC	29730	0.0	17.729795	10-11
CAGAGTA	32950	0.0	17.43004	10-11
CGTCGAA	425	3.5519275E-5	16.883024	94
ATGGGGG	15300	0.0	16.04656	5
>>END_MODULE