FastQCFastQC Report
Fri 27 May 2016
SRR2140033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8165863
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78750.043.825721
GTATCAA138550.031.9576591
ATCAACG161900.029.8442573
TCAACGC163800.029.5494694
CAACGCA167750.028.853675
AACGCAG174250.027.8253176
TATCAAC182400.026.7900282
ACGCAGA198700.024.4014197
CGCAGAG208200.023.2677848
TACATGG212800.021.8552822
ACATGGG215800.020.9663473
TTCAACG9700.020.3935051
GTACATG215300.020.3699261
GCAGAGT259700.018.7670969
CATGGGG151950.018.4198764
GAGTACT150400.018.07778212-13
CAGAGTA251200.017.1737310-11
AGAGTAC232000.017.1618310-11
ATGGGGG95500.015.9099795
GTACTTT173950.015.55823414-15
Produced by FastQC (version 0.11.5)