##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8165863
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.24529091413853	18.0	18.0	18.0	18.0	32.0
2	29.74651007493023	32.0	27.0	32.0	27.0	33.0
3	31.739995393016024	33.0	32.0	33.0	29.0	33.0
4	32.26173314443311	33.0	33.0	33.0	32.0	33.0
5	32.85347378470591	33.0	33.0	34.0	32.0	34.0
6	36.90734463705796	38.0	37.0	38.0	35.0	38.0
7	37.178056256883075	38.0	38.0	38.0	36.0	38.0
8	37.21550435024442	38.0	38.0	38.0	36.0	38.0
9	37.33704141252431	38.0	38.0	38.0	37.0	38.0
10-11	37.39237224528503	38.0	38.0	38.0	37.0	38.0
12-13	37.407952349923086	38.0	38.0	38.0	37.0	38.0
14-15	37.40948599064177	38.0	38.0	38.0	37.0	38.0
16-17	37.367129108093046	38.0	38.0	38.0	37.0	38.0
18-19	37.354101152566486	38.0	38.0	38.0	37.0	38.0
20-21	37.40188846465419	38.0	38.0	38.0	37.0	38.0
22-23	37.419300876410304	38.0	38.0	38.0	37.0	38.0
24-25	37.424781999049785	38.0	38.0	38.0	37.0	38.0
26-27	37.330318005331335	38.0	38.0	38.0	37.0	38.0
28-29	37.23912397378043	38.0	38.0	38.0	37.0	38.0
30-31	37.322335061359325	38.0	38.0	38.0	37.0	38.0
32-33	37.345026920300015	38.0	38.0	38.0	37.0	38.0
34-35	37.301570392434655	38.0	38.0	38.0	37.0	38.0
36-37	37.30261630554628	38.0	38.0	38.0	37.0	38.0
38-39	37.30140437645566	38.0	38.0	38.0	37.0	38.0
40-41	37.262363429228174	38.0	38.0	38.0	37.0	38.0
42-43	37.26334320087561	38.0	38.0	38.0	37.0	38.0
44-45	37.19947973919415	38.0	38.0	38.0	37.0	38.0
46-47	37.1795005677791	38.0	38.0	38.0	37.0	38.0
48-49	37.156978358633076	38.0	38.0	38.0	36.5	38.0
50-51	37.14951936949382	38.0	38.0	38.0	36.0	38.0
52-53	37.12866985283692	38.0	38.0	38.0	36.0	38.0
54-55	37.080595904725	38.0	38.0	38.0	36.0	38.0
56-57	37.08118296388105	38.0	38.0	38.0	36.0	38.0
58-59	37.08854057558477	38.0	38.0	38.0	36.0	38.0
60-61	37.09613735989734	38.0	38.0	38.0	36.0	38.0
62-63	37.026192127901524	38.0	38.0	38.0	36.0	38.0
64-65	37.05386142250421	38.0	38.0	38.0	36.0	38.0
66-67	37.07156952582511	38.0	38.0	38.0	36.0	38.0
68-69	37.04196918945519	38.0	38.0	38.0	36.0	38.0
70-71	36.995580494030214	38.0	38.0	38.0	36.0	38.0
72-73	37.00645154470857	38.0	38.0	38.0	36.0	38.0
74-75	37.02435256443661	38.0	38.0	38.0	36.0	38.0
76-77	36.96144997935468	38.0	38.0	38.0	36.0	38.0
78-79	36.98519996689136	38.0	38.0	38.0	36.0	38.0
80-81	36.96032770561482	38.0	38.0	38.0	36.0	38.0
82-83	36.91100582452448	38.0	38.0	38.0	36.0	38.0
84-85	36.93648620000924	38.0	38.0	38.0	36.0	38.0
86-87	36.9540073783404	38.0	38.0	38.0	36.0	38.0
88-89	36.867726152865586	38.0	38.0	38.0	35.5	38.0
90-91	36.82630057443256	38.0	38.0	38.0	35.0	38.0
92-93	36.87515691878751	38.0	38.0	38.0	35.0	38.0
94-95	36.85689267808131	38.0	38.0	38.0	35.0	38.0
96-97	36.80807496396439	38.0	38.0	38.0	35.0	38.0
98-99	36.818384831876344	38.0	38.0	38.0	35.0	38.0
100	35.20478291782931	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	12.0
19	28.0
20	92.0
21	301.0
22	760.0
23	1805.0
24	3828.0
25	7390.0
26	12894.0
27	21077.0
28	31971.0
29	46860.0
30	64161.0
31	85775.0
32	115301.0
33	157220.0
34	238332.0
35	450301.0
36	1538960.0
37	5388794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.70892764181814	14.950814139301627	22.07546220160686	24.264796017273373
2	14.499571692125318	21.727241054366015	40.95151089506243	22.82167635844624
3	18.239970472196266	26.147732333986013	30.64191745563206	24.970379738185663
4	11.906060575630876	18.0006314094612	39.71226293672998	30.381045078177944
5	12.694636723224242	37.87409249539015	36.56538031642509	12.865890464960522
6	29.489740973149953	38.68597361714353	19.054626760062323	12.769658649644194
7	26.036446126134155	32.66556949720305	24.005698847923078	17.29228552873971
8	24.164586155804965	36.9732629606938	21.823180232144477	17.038970651356752
9	25.65941897384269	16.156553202031432	21.439436346164513	36.744591477961364
10-11	23.816851935919082	26.872162807531797	29.71812654706551	19.592858709483615
12-13	24.56914229396207	24.488979303228575	29.719136850569257	21.2227415522401
14-15	21.644448357754715	25.861632750880098	27.14858796920791	25.345330922157277
16-17	21.428286269314096	28.918553985047264	27.855714699107736	21.797445046530907
18-19	21.410988648719677	28.287365340319816	29.386569429342618	20.91507658161789
20-21	22.1415799348763	27.563656278446004	29.21114085752204	21.08362292915566
22-23	21.84520309702558	27.46523341281113	29.092168732317887	21.597394757845407
24-25	21.612164226860624	27.506131409657776	29.232564904147925	21.649139459333675
26-27	21.64343255092022	27.706627908571924	29.079062054760424	21.570877485747435
28-29	21.55932972657165	27.783376922933652	29.084564478135437	21.57272887235926
30-31	21.789487062846298	27.64083971394824	29.064167075192866	21.5055061480126
32-33	21.561434806262348	27.754686337553036	29.088679389238358	21.595199466946262
34-35	21.68844297705511	27.781620829386313	29.084900416591182	21.4450357769674
36-37	21.699629973881056	27.67254593270152	29.05785037961205	21.56997371380538
38-39	21.500070928259404	27.82085181034245	29.164109527206072	21.514967734192076
40-41	21.72844691006574	27.69327492292139	29.043854332748225	21.53442383426465
42-43	21.609020335967593	27.747963555864285	28.948416120824533	21.694599987343587
44-45	21.715749577937686	27.87315732252077	28.82459112054749	21.586501978994054
46-47	21.714176920093752	27.862781509695267	28.661489774981924	21.761551795229053
48-49	21.682884972478995	27.925860700217385	28.696248147442887	21.695006179860734
50-51	21.648035364433827	28.034208922612798	28.586077392798202	21.731678320155176
52-53	21.78253955805947	27.95941975824212	28.51528346293194	21.742757220766475
54-55	21.799737028434713	27.903965995282647	28.51071160523684	21.785585371045805
56-57	21.763754494188696	27.986455964224305	28.49307175370012	21.756717787886878
58-59	21.731716348204152	27.980945223909888	28.540898518641605	21.74643990924435
60-61	21.76246947484932	27.969400888715047	28.57352298738416	21.694606649051472
62-63	21.761076762470267	27.965752969881873	28.530666600455408	21.74250366719245
64-65	21.707569402644765	27.98888160631693	28.569158160657043	21.734390830381262
66-67	21.792503865224504	28.01002557610251	28.414491488681392	21.782979069991594
68-69	21.76549246490334	28.030398520397494	28.440831179162867	21.7632778355363
70-71	21.786909952397803	27.810067042407017	28.504158784410922	21.898864220784258
72-73	21.796578215755055	27.840931683752313	28.501636289802452	21.86085381069018
74-75	21.702994483260447	28.00396492452536	28.449788760107214	21.843251832106976
76-77	21.833366556498802	27.927220335908338	28.35202170776874	21.88739139982413
78-79	21.78162471479198	27.942102771979222	28.34948753580271	21.926784977426088
80-81	21.79315854282918	27.96887963096026	28.29709040462449	21.940871421586074
82-83	21.83815376458639	27.943702242684765	28.374107867897397	21.844036124831444
84-85	21.92591642056512	27.925065696583008	28.328865450621336	21.820152432230532
86-87	21.78684486432162	27.972408197096353	28.367718316727846	21.87302862185418
88-89	21.85828193300092	27.956551822981712	28.273835384483675	21.911330859533688
90-91	21.895985768788886	27.96751878860173	28.295424150463884	21.841071292145504
92-93	21.90955538488483	27.997228634229128	28.27416003439094	21.819055946495112
94-95	21.79306813010738	28.047654290370318	28.30089546764653	21.858382111875773
96-97	21.901687361250623	28.166009673416674	28.339219542665166	21.593083422667533
98-99	22.945176900443855	30.054100680948626	28.59952299030412	18.401199428303396
100	28.918501987600564	41.1245980456752	29.956899966724237	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1238.0
1	1466.0
2	2301.0
3	3152.0
4	3875.0
5	4847.5
6	5866.5
7	7744.5
8	9327.5
9	9657.0
10	9509.5
11	9543.5
12	10485.5
13	12456.5
14	16521.0
15	21071.0
16	23917.0
17	24908.5
18	24435.5
19	23641.5
20	23460.0
21	24949.0
22	29145.0
23	36730.0
24	47716.5
25	61759.5
26	79540.5
27	100049.5
28	120318.0
29	141743.0
30	162312.5
31	180743.0
32	199570.0
33	217684.0
34	234853.5
35	252645.0
36	271221.0
37	287707.0
38	300679.5
39	311924.5
40	323533.5
41	332494.0
42	337698.0
43	342578.0
44	348053.5
45	351246.0
46	351298.5
47	350189.5
48	345949.0
49	336588.0
50	325276.0
51	312574.0
52	296554.0
53	278732.5
54	258579.5
55	236513.0
56	214944.5
57	193197.5
58	170400.0
59	146170.5
60	122173.5
61	99505.0
62	78905.5
63	61383.0
64	46699.0
65	35084.5
66	25764.0
67	19173.5
68	14341.5
69	10412.5
70	7483.5
71	5254.0
72	3701.5
73	2547.0
74	1685.0
75	1070.5
76	690.5
77	443.0
78	285.5
79	182.0
80	113.5
81	76.0
82	55.0
83	37.0
84	24.0
85	15.5
86	11.5
87	9.0
88	7.0
89	5.5
90	6.0
91	6.0
92	4.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	9.796882460555607E-5
3	0.0
4	6.123051537847255E-5
5	0.0024737128212902913
6	3.673830922708353E-4
7	2.449220615138902E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0010966716016888743
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.0426834187660337E-4
50-51	4.540427771467241E-4
52-53	1.7928913054997804E-4
54-55	2.94825326380012E-4
56-57	4.127281854039963E-4
58-59	3.554421786144795E-4
60-61	4.143449537756769E-5
62-63	4.0451940235466564E-4
64-65	9.154062520134536E-5
66-67	1.067003555255846E-4
68-69	4.889417553167203E-4
70-71	9.452517459890423E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.001114191391629056
90-91	0.0
92-93	8.207076354616936E-6
94-95	7.474890596670684E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	278.0
22-23	428.0
24-25	617.0
26-27	2093.0
28-29	6959.0
30-31	17468.0
32-33	22519.0
34-35	31607.0
36-37	52715.0
38-39	63122.0
40-41	59629.0
42-43	63951.0
44-45	74672.0
46-47	78053.0
48-49	75326.0
50-51	70175.0
52-53	66765.0
54-55	70952.0
56-57	75022.0
58-59	74437.0
60-61	72758.0
62-63	68312.0
64-65	70389.0
66-67	74528.0
68-69	77301.0
70-71	75469.0
72-73	69228.0
74-75	68729.0
76-77	71891.0
78-79	74768.0
80-81	74950.0
82-83	69411.0
84-85	66762.0
86-87	69013.0
88-89	71791.0
90-91	73394.0
92-93	71324.0
94-95	80099.0
96-97	239060.0
98-99	1807007.0
100-101	3912758.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.14491510448623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.32776736948844	80.50078301465827
2	6.345145923738047	11.18584697546934
3	1.396632720835996	3.693182178307095
4	0.4680041654590609	1.650087497317394
5	0.1964200554692287	0.8656714557076821
6	0.10121872829040096	0.535314972728487
7	0.04927449632322858	0.30403074066591107
8	0.032688505861745945	0.23050604592608775
9	0.020701513349844075	0.1642259823080755
>10	0.06119956327772631	0.7769227632830413
>50	7.149696125065577E-4	0.042643721863921846
>100	1.9291601968290717E-4	0.025763840508894978
>500	3.907227415090006E-5	0.025020811255794483
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2246103075694511E-5	0.0	1.2246103075694511E-5	0.0
3	0.0	1.2246103075694511E-5	0.0	1.2246103075694511E-5	0.0
4	0.0	3.673830922708353E-5	0.0	1.2246103075694511E-5	0.0
5	0.0	3.673830922708353E-5	0.0	1.2246103075694511E-5	0.0
6	3.673830922708353E-5	3.673830922708353E-5	0.0	1.2246103075694511E-5	0.0
7	3.673830922708353E-5	3.673830922708353E-5	0.0	2.4492206151389022E-5	0.0
8	3.673830922708353E-5	4.8984412302778044E-5	0.0	3.673830922708353E-5	0.0
9	3.673830922708353E-5	7.347661845416706E-5	0.0	8.572272152986158E-5	0.0
10-11	3.673830922708353E-5	7.347661845416706E-5	0.0	8.572272152986158E-5	0.0
12-13	3.673830922708353E-5	7.347661845416706E-5	0.0	8.572272152986158E-5	0.0
14-15	4.286136076493078E-5	1.0409187614340334E-4	0.0	8.572272152986158E-5	0.0
16-17	6.73535669163198E-5	1.102149276812506E-4	0.0	9.796882460555609E-5	0.0
18-19	7.347661845416706E-5	1.1633797921909784E-4	0.0	9.796882460555609E-5	0.0
20-21	7.347661845416706E-5	1.2858408229479235E-4	0.0	1.0409187614340334E-4	0.0
22-23	8.572272152986158E-5	1.5307628844618136E-4	0.0	1.224610307569451E-4	0.0
24-25	9.796882460555609E-5	1.775684945975704E-4	0.0	1.469532369083341E-4	0.0
26-27	1.102149276812506E-4	1.8369154613541766E-4	0.0	1.8369154613541766E-4	0.0
28-29	1.102149276812506E-4	1.8369154613541766E-4	0.0	3.673830922708353E-4	0.0
30-31	1.224610307569451E-4	2.571681645895847E-4	0.0	0.001126641482963895	0.0
32-33	1.224610307569451E-4	2.571681645895847E-4	0.0	0.002994172202007308	0.0
34-35	1.347071338326396E-4	3.1839867996805724E-4	0.0	0.005180101601018778	0.0
36-37	1.469532369083341E-4	3.9187529842222435E-4	0.0	0.008511041637607686	0.0
38-39	1.469532369083341E-4	4.102444530357661E-4	0.0	0.014346309753176118	0.0
40-41	1.5919933998402862E-4	4.286136076493079E-4	0.0	0.02023668533258518	0.0
42-43	1.8369154613541766E-4	4.408597107250024E-4	0.0	0.025759677819723402	0.0
44-45	1.8369154613541766E-4	4.7759801995208586E-4	0.0	0.03194395987294913	0.0
46-47	1.8369154613541766E-4	5.143363291791694E-4	0.0	0.038979346089935626	0.0
48-49	1.8369154613541766E-4	5.143363291791694E-4	0.0	0.04591676348231657	0.0
50-51	2.0206070074895943E-4	6.00059050709031E-4	0.0	0.05289091918392459	0.0
52-53	2.204298553625012E-4	6.00059050709031E-4	0.0	0.05958341451479164	0.0
54-55	2.204298553625012E-4	6.00059050709031E-4	0.0	0.06605547999029619	0.0
56-57	2.204298553625012E-4	6.00059050709031E-4	0.0	0.07236222307427886	0.0
58-59	2.204298553625012E-4	6.00059050709031E-4	0.0	0.07886490380747264	0.0
60-61	2.326759584381957E-4	6.061821022468782E-4	0.0	0.08531860012836365	0.0
62-63	2.449220615138902E-4	6.123051537847255E-4	0.0	0.09151512828466507	0.0
64-65	2.449220615138902E-4	6.857817722388926E-4	0.0	0.09717282790563594	0.0
66-67	2.571681645895847E-4	6.857817722388926E-4	0.0	0.10311831094888563	0.0
68-69	2.571681645895847E-4	7.041509268524344E-4	0.0	0.10927197774442211	0.0
70-71	2.571681645895847E-4	7.225200814659762E-4	0.0	0.11466638614926555	0.0
72-73	3.1227562843021E-4	7.225200814659762E-4	0.0	0.11883618424653952	0.0
74-75	3.1839867996805724E-4	7.286431330038234E-4	0.0	0.12198955578853085	0.0
76-77	3.1839867996805724E-4	7.408892360795179E-4	0.0	0.12506945071206804	0.0
78-79	3.1839867996805724E-4	7.470122876173652E-4	0.0	0.12720027264723888	0.0
80-81	3.245217315059045E-4	7.470122876173652E-4	0.0	0.12787380831640208	0.0
82-83	3.3064478304375175E-4	7.470122876173652E-4	0.0	0.12840039074865695	0.0
84-85	3.3064478304375175E-4	7.531353391552124E-4	0.0	0.12874940468631424	0.0
86-87	3.36767834581599E-4	7.837505968444487E-4	0.0	0.12888411182014686	0.0
88	3.428908861194463E-4	8.082428029958377E-4	0.0	0.12890248097476042	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7875	0.0	43.82572	1
GTATCAA	13855	0.0	31.957659	1
ATCAACG	16190	0.0	29.844257	3
TCAACGC	16380	0.0	29.549469	4
CAACGCA	16775	0.0	28.85367	5
AACGCAG	17425	0.0	27.825317	6
TATCAAC	18240	0.0	26.790028	2
ACGCAGA	19870	0.0	24.401419	7
CGCAGAG	20820	0.0	23.267784	8
TACATGG	21280	0.0	21.855282	2
ACATGGG	21580	0.0	20.966347	3
TTCAACG	970	0.0	20.393505	1
GTACATG	21530	0.0	20.369926	1
GCAGAGT	25970	0.0	18.767096	9
CATGGGG	15195	0.0	18.419876	4
GAGTACT	15040	0.0	18.077782	12-13
CAGAGTA	25120	0.0	17.17373	10-11
AGAGTAC	23200	0.0	17.16183	10-11
ATGGGGG	9550	0.0	15.909979	5
GTACTTT	17395	0.0	15.558234	14-15
>>END_MODULE