FastQCFastQC Report
Fri 27 May 2016
SRR2140032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15627441
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131650.040.809821
GTATCAA233400.030.1030351
ATCAACG283050.029.7870713
TCAACGC289250.029.1485924
CAACGCA296650.028.3789275
AACGCAG307000.027.4493246
TATCAAC322850.026.4278452
ACGCAGA347000.024.212397
TTCAACG24800.023.071821
CGCAGAG364800.023.0425058
TCGCAGA52250.021.5795861
ACATGGG376700.021.0527573
TACATGG387050.020.9463962
GTACATG367950.018.694921
GCAGAGT454250.018.5605959
CATGGGG270550.018.3496484
AGAGTAC403150.017.53722610-11
GAGTACT264600.017.51393512-13
CAGAGTA446800.016.76555310-11
GTACTTT309550.015.03226914-15
Produced by FastQC (version 0.11.5)