##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140032_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15627441
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.124221105681986	18.0	18.0	18.0	18.0	32.0
2	29.214109143013243	28.0	27.0	32.0	27.0	33.0
3	31.502505112641284	32.0	32.0	33.0	27.0	33.0
4	32.11830177442359	33.0	32.0	33.0	31.0	33.0
5	32.745088719259925	33.0	33.0	34.0	32.0	34.0
6	36.786696043197345	38.0	37.0	38.0	35.0	38.0
7	37.111518450141645	38.0	38.0	38.0	36.0	38.0
8	37.12593079058817	38.0	38.0	38.0	36.0	38.0
9	37.281929075912046	38.0	38.0	38.0	37.0	38.0
10-11	37.35075154019138	38.0	38.0	38.0	37.0	38.0
12-13	37.36772514450702	38.0	38.0	38.0	37.0	38.0
14-15	37.37073772986889	38.0	38.0	38.0	37.0	38.0
16-17	37.3239197639588	38.0	38.0	38.0	37.0	38.0
18-19	37.31680481148513	38.0	38.0	38.0	37.0	38.0
20-21	37.36542062813655	38.0	38.0	38.0	37.0	38.0
22-23	37.381983183210465	38.0	38.0	38.0	37.0	38.0
24-25	37.39097725617634	38.0	38.0	38.0	37.0	38.0
26-27	37.29679129310367	38.0	38.0	38.0	37.0	38.0
28-29	37.20733264390321	38.0	38.0	38.0	36.5	38.0
30-31	37.2888012807705	38.0	38.0	38.0	37.0	38.0
32-33	37.3129773013119	38.0	38.0	38.0	37.0	38.0
34-35	37.2704149357107	38.0	38.0	38.0	37.0	38.0
36-37	37.27568836573949	38.0	38.0	38.0	37.0	38.0
38-39	37.274544548997326	38.0	38.0	38.0	37.0	38.0
40-41	37.23328280579438	38.0	38.0	38.0	37.0	38.0
42-43	37.22667609941462	38.0	38.0	38.0	37.0	38.0
44-45	37.16279758076227	38.0	38.0	38.0	36.5	38.0
46-47	37.14093188851122	38.0	38.0	38.0	36.0	38.0
48-49	37.11552614909756	38.0	38.0	38.0	36.0	38.0
50-51	37.101039034488174	38.0	38.0	38.0	36.0	38.0
52-53	37.07309182006365	38.0	38.0	38.0	36.0	38.0
54-55	37.01991000000031	38.0	38.0	38.0	36.0	38.0
56-57	37.0124592574006	38.0	38.0	38.0	36.0	38.0
58-59	37.015953769788936	38.0	38.0	38.0	36.0	38.0
60-61	37.01981543929031	38.0	38.0	38.0	36.0	38.0
62-63	36.9443329838232	38.0	38.0	38.0	36.0	38.0
64-65	36.97030518099738	38.0	38.0	38.0	36.0	38.0
66-67	36.98039071213222	38.0	38.0	38.0	36.0	38.0
68-69	36.945665707879556	38.0	38.0	38.0	36.0	38.0
70-71	36.89572836659757	38.0	38.0	38.0	35.0	38.0
72-73	36.8978259661023	38.0	38.0	38.0	35.0	38.0
74-75	36.91440522782676	38.0	38.0	38.0	35.0	38.0
76-77	36.843125872307155	38.0	38.0	38.0	35.0	38.0
78-79	36.86170031779871	38.0	38.0	38.0	35.0	38.0
80-81	36.83449366332911	38.0	38.0	38.0	35.0	38.0
82-83	36.78118815420477	38.0	38.0	38.0	35.0	38.0
84-85	36.80135068048851	38.0	38.0	38.0	35.0	38.0
86-87	36.814985297968505	38.0	38.0	38.0	35.0	38.0
88-89	36.72161022468414	38.0	38.0	38.0	34.5	38.0
90-91	36.673863775688815	38.0	38.0	38.0	34.0	38.0
92-93	36.71778478736524	38.0	38.0	38.0	34.5	38.0
94-95	36.698613080227105	38.0	38.0	38.0	34.0	38.0
96-97	36.64354477121557	38.0	38.0	38.0	34.0	38.0
98-99	36.655563949865524	38.0	38.0	38.0	34.0	38.0
100	34.890791776659405	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	24.0
19	81.0
20	253.0
21	642.0
22	1671.0
23	3997.0
24	8418.0
25	15744.0
26	27177.0
27	42901.0
28	66052.0
29	94691.0
30	131311.0
31	177109.0
32	236922.0
33	328204.0
34	502593.0
35	943624.0
36	3173096.0
37	9872929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.808933785128346	12.034964649682568	28.524708555930555	23.631393009258524
2	14.445371233346997	21.94319157302526	40.95069984293648	22.660737350691267
3	18.09328859408268	26.312337381404927	30.824989196887703	24.769384827624688
4	11.89268516054903	18.221937594633232	39.84845756546914	30.03691967934859
5	12.665378992030274	37.90235779607984	36.676208615577416	12.756054596312467
6	29.320370978987686	38.97798662452532	19.137364302512612	12.564278093974382
7	25.87908380358005	32.71215013852241	24.195211818190256	17.213554239707285
8	24.07655866369932	37.00960381165413	21.949351784466824	16.96448574017973
9	25.61645249532537	16.115172023365822	21.372513900388427	36.89586158092038
10-11	23.64539082246415	26.81065953152535	30.0565780411521	19.487371604858403
12-13	24.515821240342547	24.483714256224033	29.868626603677466	21.131837899755947
14-15	21.639700959357324	25.86359148628365	27.171383977709468	25.32532357664956
16-17	21.432187777896587	28.95560443965202	27.839570790892765	21.77263699155863
18-19	21.49033229432765	28.325520473889487	29.28964505449101	20.894502177291855
20-21	22.189803259975847	27.618972109539214	29.070357648226924	21.120866982258015
22-23	21.891411890012353	27.53762359992188	28.96112968197618	21.609834828089593
24-25	21.676809676108142	27.61231890631553	29.06333706786535	21.647534349710973
26-27	21.71611005802935	27.750150671246683	28.901306837253642	21.63243243347032
28-29	21.64416206133212	27.793436242508037	28.948510366260805	21.613891329899044
30-31	21.87226856663032	27.681046608491407	28.906116814323152	21.540568010555123
32-33	21.66138014929329	27.83167267663378	28.88528178035218	21.621665393720757
34-35	21.748053841251288	27.845123379458236	28.901768203097124	21.50505457619335
36-37	21.753586426718886	27.75245906832029	28.911596484978908	21.582358019981914
38-39	21.58663087551663	27.86261242688632	29.011042664936692	21.53971403266036
40-41	21.823393880130595	27.716554697522884	28.906492044948262	21.55355937739826
42-43	21.70282660504463	27.79979087949037	28.778115785451565	21.71926673001343
44-45	21.787204317729906	27.88228530389809	28.66559282922844	21.664917549143563
46-47	21.828561950587904	27.851627199963776	28.51997493944808	21.799835910000244
48-49	21.769837127463852	27.901011802212512	28.568677370823025	21.76047369950061
50-51	21.722427120567154	28.03850417087583	28.48887806004662	21.7501906485104
52-53	21.906141480127463	27.941567371111635	28.387819081981508	21.764472066779394
54-55	21.874679942697554	27.875582134446457	28.409939739851826	21.839798183004167
56-57	21.86464233428	27.949914460596194	28.41845614722323	21.76698705790058
58-59	21.842398731345387	27.937618530184643	28.423815912245438	21.79616682622453
60-61	21.85713793120363	27.933411751262906	28.4480636792446	21.76138663828886
62-63	21.886097953260457	27.920008571358235	28.41128476706198	21.78260870831933
64-65	21.865392739152842	27.90567168142852	28.429494462434906	21.79944111698373
66-67	21.8930017865191	27.939563567129923	28.307917561244366	21.859517085106614
68-69	21.8859192528138	27.937068628886518	28.346184233249065	21.83082788505061
70-71	21.91046091269979	27.711140773641223	28.408015498969142	21.97038281468985
72-73	21.92293720045599	27.754137222783303	28.376449626615624	21.946475950145082
74-75	21.77836773546353	27.912522084207904	28.31986625973681	21.989243920591758
76-77	21.925760545253073	27.852863005408224	28.235287628607843	21.986088820730863
78-79	21.912389495698577	27.905480600510852	28.195994751558516	21.98613515223205
80-81	21.914667381802186	27.874765011237514	28.199663256589435	22.01090435037087
82-83	21.97798887097375	27.85378086305861	28.23734530331209	21.930884962655554
84-85	22.02938061020639	27.841025494530232	28.196497865003874	21.933096030259513
86-87	21.88391173398863	27.86599341469409	28.239654123799106	22.010440727518173
88-89	21.96498213455593	27.85574126278924	28.155368629515355	22.023907973139472
90-91	22.021761942879685	27.90994921773995	28.164458264474558	21.903830574905808
92-93	22.03649197565834	27.874723381469042	28.119103410173167	21.969681232699454
94-95	21.941353351326665	27.928320570489973	28.156094913372737	21.974231164810625
96-97	22.03072047149836	28.063227880981312	28.20867161782743	21.697380029692894
98-99	23.073470311458493	29.98297395022465	28.439905896838937	18.503649841477916
100	29.1502011677674	40.98448447485216	29.865314357380445	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2078.0
1	2802.5
2	4661.5
3	6082.5
4	7209.5
5	8679.0
6	9923.5
7	13220.0
8	16462.5
9	17115.5
10	17073.0
11	17324.0
12	18623.5
13	22018.5
14	29627.5
15	38230.0
16	42633.0
17	43051.0
18	41873.0
19	40960.5
20	41886.0
21	46025.5
22	54695.5
23	69849.5
24	91767.5
25	120747.0
26	157861.0
27	198475.0
28	237736.5
29	277941.5
30	314546.5
31	347132.0
32	382730.0
33	419731.0
34	455351.5
35	489083.0
36	523576.0
37	555030.0
38	579865.5
39	599730.5
40	620305.0
41	639640.5
42	651157.0
43	660791.0
44	671268.5
45	676141.0
46	674124.0
47	670480.5
48	660228.0
49	643100.5
50	623800.5
51	598485.5
52	566792.5
53	531932.5
54	493824.0
55	451895.0
56	408627.5
57	365240.0
58	320458.5
59	273121.0
60	227029.0
61	184365.0
62	146218.5
63	113485.5
64	85111.0
65	63270.0
66	47274.0
67	36127.5
68	27456.0
69	20042.0
70	14503.0
71	10126.5
72	7006.5
73	4770.0
74	3205.0
75	2152.5
76	1365.0
77	801.5
78	502.0
79	329.5
80	205.0
81	124.5
82	76.5
83	49.0
84	34.5
85	22.0
86	13.0
87	9.0
88	7.5
89	6.0
90	4.0
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.839400193544164E-5
3	0.0
4	1.919700096772082E-5
5	0.00195169509838495
6	2.11167010644929E-4
7	3.839400193544164E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	9.956746420845802E-4
30-31	0.0
32-33	0.0
34-35	6.447142537779046E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.9376123322854E-6
44-45	6.681971499654241E-6
46-47	1.3495906371069878E-5
48-49	1.1925749195156743E-4
50-51	4.953448420515574E-4
52-53	2.1864658320290312E-4
54-55	3.186338102686223E-4
56-57	4.984912508244992E-4
58-59	4.213595286600895E-4
60-61	5.049600421511788E-5
62-63	4.042390031422481E-4
64-65	1.2131554577842121E-4
66-67	1.5966288110926344E-4
68-69	6.229214686656993E-4
70-71	1.4794572804434185E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	8.977424574810382E-4
90-91	0.0
92-93	1.2822737072212655E-5
94-95	9.51600063601488E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	204.0
20-21	467.0
22-23	806.0
24-25	1158.0
26-27	4460.0
28-29	14486.0
30-31	35801.0
32-33	45697.0
34-35	62854.0
36-37	102799.0
38-39	121325.0
40-41	113994.0
42-43	123124.0
44-45	143661.0
46-47	147045.0
48-49	140983.0
50-51	131147.0
52-53	124862.0
54-55	134789.0
56-57	140672.0
58-59	139754.0
60-61	135632.0
62-63	128637.0
64-65	132322.0
66-67	140898.0
68-69	143595.0
70-71	140899.0
72-73	130708.0
74-75	129405.0
76-77	136001.0
78-79	140681.0
80-81	141104.0
82-83	131185.0
84-85	126981.0
86-87	130945.0
88-89	136889.0
90-91	139282.0
92-93	136777.0
94-95	156023.0
96-97	463796.0
98-99	3493281.0
100-101	7482312.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.03606199393512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.23896345699242	77.63805054254246
2	6.833949115991422	11.759321396136722
3	1.6637026461878863	4.294152720206848
4	0.6042066475862984	2.0793424235552993
5	0.26910426333780707	1.1576335541681908
6	0.13711650617234258	0.7078178535261272
7	0.0765232433789425	0.4608630957929377
8	0.04915442909449295	0.3383242807080227
9	0.03252914988935661	0.2518811960841623
>10	0.09327586463674091	1.183521278138325
>50	0.001095815046712921	0.06107453944646048
>100	3.444122481237884E-4	0.04216480742825345
>500	2.2982882628425623E-5	0.013244366478133134
>1k	1.1466554910269654E-5	0.012607945788112973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.399000322573607E-6	0.0	1.919700096772082E-5	0.0
3	0.0	6.399000322573607E-6	0.0	2.5596001290294427E-5	0.0
4	0.0	1.2798000645147213E-5	0.0	3.199500161286803E-5	0.0
5	1.2798000645147213E-5	1.2798000645147213E-5	0.0	3.839400193544164E-5	0.0
6	1.919700096772082E-5	1.2798000645147213E-5	0.0	3.839400193544164E-5	6.399000322573607E-6
7	1.919700096772082E-5	1.919700096772082E-5	0.0	5.7591002903162456E-5	6.399000322573607E-6
8	1.919700096772082E-5	1.919700096772082E-5	0.0	5.7591002903162456E-5	6.399000322573607E-6
9	1.919700096772082E-5	7.678800387088328E-5	0.0	1.2158100612889852E-4	1.2798000645147213E-5
10-11	1.919700096772082E-5	8.318700419345688E-5	0.0	1.3437900677404574E-4	1.2798000645147213E-5
12-13	2.5596001290294427E-5	9.278550467731728E-5	0.0	1.3757850693533254E-4	1.2798000645147213E-5
14-15	2.5596001290294427E-5	1.4077800709661934E-4	0.0	1.5997500806434016E-4	2.5596001290294427E-5
16-17	2.8795501451581228E-5	1.5357600774176656E-4	0.0	1.6317450822562695E-4	3.839400193544164E-5
18-19	3.199500161286803E-5	1.6317450822562698E-4	0.0	1.7917200903206097E-4	3.839400193544164E-5
20-21	3.839400193544164E-5	1.8237150919334777E-4	0.0	2.207655111287894E-4	4.479300225801524E-5
22-23	5.119200258058885E-5	1.983690099997818E-4	0.0	2.4636151241908384E-4	5.7591002903162456E-5
24-25	6.718950338702287E-5	2.3356351177393662E-4	0.0	2.751570138706651E-4	7.998750403217008E-5
26-27	7.678800387088328E-5	2.5596001290294424E-4	0.0	3.3914701709640116E-4	8.638650435474369E-5
28-29	7.678800387088328E-5	2.5915951306423104E-4	0.0	6.49498532741221E-4	9.278550467731728E-5
30-31	8.318700419345688E-5	3.0395251532224627E-4	0.0	0.0017085330861271529	9.918450499989091E-5
32-33	8.318700419345688E-5	3.135510158061067E-4	0.0	0.003909789197092474	1.023840051611777E-4
34-35	8.318700419345688E-5	3.935385198382768E-4	0.0	0.006616566333541109	1.119825056450381E-4
36-37	8.318700419345688E-5	4.6712702354787325E-4	0.0	0.0109582880524073	1.1518200580632491E-4
38-39	8.318700419345688E-5	5.087205256446017E-4	0.0	0.017357288374980906	1.1518200580632491E-4
40-41	8.958600451603049E-5	5.727105288703378E-4	0.0	0.02417862271884437	1.4397750725790614E-4
42-43	8.958600451603049E-5	5.951070299993453E-4	0.0	0.030622416043675993	1.5037650758047973E-4
44-45	1.055835053224645E-4	6.239025314509266E-4	0.0	0.03783408940721644	1.5357600774176656E-4
46-47	1.2158100612889852E-4	6.49498532741221E-4	0.0	0.045349715286079145	1.6317450822562695E-4
48-49	1.2478050629018532E-4	6.590970332250814E-4	0.0	0.05306371017494163	1.8557100935463457E-4
50-51	1.3437900677404574E-4	7.294860367733911E-4	3.1995001612868033E-6	0.06110085458009408	1.8557100935463457E-4
52-53	1.4717700741919294E-4	7.422840374185383E-4	6.399000322573607E-6	0.06834132344508612	2.047680103223554E-4
54-55	1.4717700741919294E-4	7.422840374185383E-4	6.399000322573607E-6	0.07575136581862635	2.2396501129007623E-4
56-57	1.4717700741919294E-4	7.422840374185383E-4	6.399000322573607E-6	0.08335657770200508	2.2396501129007623E-4
58-59	1.4717700741919294E-4	7.614810383862592E-4	6.399000322573607E-6	0.0907698195757066	2.2716451145136303E-4
60-61	1.4717700741919294E-4	7.838775395152668E-4	6.399000322573607E-6	0.09827264745392415	2.3676301193522342E-4
62-63	1.4717700741919294E-4	7.902765398378404E-4	6.399000322573607E-6	0.10544272731536788	2.4316201225779705E-4
64-65	1.4717700741919294E-4	8.510670429022896E-4	6.399000322573607E-6	0.11202089964697355	2.4636151241908384E-4
66-67	1.4717700741919294E-4	8.542665430635764E-4	6.399000322573607E-6	0.11864706448099852	2.4956101258037064E-4
68-69	1.5997500806434016E-4	8.76663044192584E-4	6.399000322573607E-6	0.12540440882163625	2.4956101258037064E-4
70-71	1.6317450822562695E-4	8.862615446764445E-4	6.399000322573607E-6	0.1314290676253393	2.4956101258037064E-4
72-73	1.6957350854820058E-4	8.894610448377313E-4	6.399000322573607E-6	0.13556922083404443	2.815560141932387E-4
74-75	1.7277300870948738E-4	9.054585456441653E-4	6.399000322573607E-6	0.1388711050004924	2.815560141932387E-4
76-77	1.7277300870948738E-4	9.438525475796069E-4	6.399000322573607E-6	0.14208340316242435	2.815560141932387E-4
78-79	1.7917200903206097E-4	9.59850048386041E-4	6.399000322573607E-6	0.14433905077613154	2.815560141932387E-4
80-81	1.7917200903206097E-4	9.662490487086146E-4	6.399000322573607E-6	0.14498534980871147	2.9435401483838587E-4
82-83	1.8877050951592137E-4	9.726480490311882E-4	6.399000322573607E-6	0.14555486083742053	3.167505159673935E-4
84-85	1.983690099997818E-4	9.854460496763354E-4	6.399000322573607E-6	0.14579482334951704	3.199500161286803E-4
86-87	1.983690099997818E-4	9.91845049998909E-4	6.399000322573607E-6	0.1459260028561298	3.231495162899671E-4
88	1.983690099997818E-4	0.0010430370525794977	6.399000322573607E-6	0.14594200035693625	3.263490164512539E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13165	0.0	40.80982	1
GTATCAA	23340	0.0	30.103035	1
ATCAACG	28305	0.0	29.787071	3
TCAACGC	28925	0.0	29.148592	4
CAACGCA	29665	0.0	28.378927	5
AACGCAG	30700	0.0	27.449324	6
TATCAAC	32285	0.0	26.427845	2
ACGCAGA	34700	0.0	24.21239	7
TTCAACG	2480	0.0	23.07182	1
CGCAGAG	36480	0.0	23.042505	8
TCGCAGA	5225	0.0	21.579586	1
ACATGGG	37670	0.0	21.052757	3
TACATGG	38705	0.0	20.946396	2
GTACATG	36795	0.0	18.69492	1
GCAGAGT	45425	0.0	18.560595	9
CATGGGG	27055	0.0	18.349648	4
AGAGTAC	40315	0.0	17.537226	10-11
GAGTACT	26460	0.0	17.513935	12-13
CAGAGTA	44680	0.0	16.765553	10-11
GTACTTT	30955	0.0	15.032269	14-15
>>END_MODULE