FastQCFastQC Report
Fri 27 May 2016
SRR2140031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7413938
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66850.043.3847161
GTATCAA120750.031.8275031
ATCAACG140500.028.9114553
TCAACGC144750.028.2079894
CAACGCA145900.027.8125425
AACGCAG151400.026.9679096
TATCAAC157000.026.14112
ACGCAGA172550.023.4914477
CGCAGAG179350.022.6242478
TACATGG181700.021.3828562
ACATGGG178950.021.1939563
GTACATG183050.019.615451
GCAGAGT221150.018.4241249
CATGGGG137700.017.974734
AGAGTAC197800.017.32194110-11
GAGTACT129850.017.06690812-13
CAGAGTA218950.016.4368910-11
TGCGCGA5601.6427584E-615.663465594
TTCAACG9700.015.6224691
ATGGGGG86350.015.1606215
Produced by FastQC (version 0.11.5)