FastQCFastQC Report
Fri 27 May 2016
SRR2140030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9470142
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93150.046.5793231
GTATCAA173400.033.9518051
ATCAACG216250.030.450243
TCAACGC221900.029.7132594
CAACGCA224900.029.3358175
TATCAAC232900.028.4742622
AACGCAG232200.028.4127986
ACGCAGA260950.025.2172347
TTCAACG14950.024.185421
CGCAGAG273350.024.042188
TACATGG274150.022.358962
ACATGGG275950.021.6581753
GTACATG274850.020.2126541
GCAGAGT328550.020.0028299
CATGGGG192100.018.8885024
AGAGTAC299650.018.14892210-11
GAGTACT191450.018.07451212-13
TCGCAGA34250.017.884541
CAGAGTA325000.017.68874710-11
GTACTTT215650.015.99715214-15
Produced by FastQC (version 0.11.5)