##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140030_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9470142
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.25115600167347	18.0	18.0	18.0	18.0	32.0
2	29.56992239398311	30.0	27.0	32.0	27.0	33.0
3	31.665986529029873	32.0	32.0	33.0	28.0	33.0
4	32.223504990738256	33.0	33.0	33.0	32.0	33.0
5	32.81977968228988	33.0	33.0	34.0	32.0	34.0
6	36.86368293104792	38.0	37.0	38.0	35.0	38.0
7	37.147032114196385	38.0	38.0	38.0	36.0	38.0
8	37.17719322476896	38.0	38.0	38.0	36.0	38.0
9	37.31055373826496	38.0	38.0	38.0	37.0	38.0
10-11	37.367011972998924	38.0	38.0	38.0	37.0	38.0
12-13	37.38357613856265	38.0	38.0	38.0	37.0	38.0
14-15	37.386839975577985	38.0	38.0	38.0	37.0	38.0
16-17	37.34442677839466	38.0	38.0	38.0	37.0	38.0
18-19	37.33362525081461	38.0	38.0	38.0	37.0	38.0
20-21	37.38193944685503	38.0	38.0	38.0	37.0	38.0
22-23	37.39713275946325	38.0	38.0	38.0	37.0	38.0
24-25	37.40462184978455	38.0	38.0	38.0	37.0	38.0
26-27	37.30893285811724	38.0	38.0	38.0	37.0	38.0
28-29	37.218578566033656	38.0	38.0	38.0	37.0	38.0
30-31	37.30143177915568	38.0	38.0	38.0	37.0	38.0
32-33	37.32380046927176	38.0	38.0	38.0	37.0	38.0
34-35	37.278811837705646	38.0	38.0	38.0	37.0	38.0
36-37	37.28073966986034	38.0	38.0	38.0	37.0	38.0
38-39	37.27975296605099	38.0	38.0	38.0	37.0	38.0
40-41	37.23816952098946	38.0	38.0	38.0	37.0	38.0
42-43	37.234325218345205	38.0	38.0	38.0	37.0	38.0
44-45	37.167961949758066	38.0	38.0	38.0	36.5	38.0
46-47	37.14392734510455	38.0	38.0	38.0	36.0	38.0
48-49	37.11887129468858	38.0	38.0	38.0	36.0	38.0
50-51	37.108105043465635	38.0	38.0	38.0	36.0	38.0
52-53	37.08758097752744	38.0	38.0	38.0	36.0	38.0
54-55	37.03712102234563	38.0	38.0	38.0	36.0	38.0
56-57	37.03746094794732	38.0	38.0	38.0	36.0	38.0
58-59	37.04338114753355	38.0	38.0	38.0	36.0	38.0
60-61	37.05058112009769	38.0	38.0	38.0	36.0	38.0
62-63	36.980600140487994	38.0	38.0	38.0	36.0	38.0
64-65	37.01024084194722	38.0	38.0	38.0	36.0	38.0
66-67	37.027550534820406	38.0	38.0	38.0	36.0	38.0
68-69	36.997438189090744	38.0	38.0	38.0	36.0	38.0
70-71	36.9505076504862	38.0	38.0	38.0	36.0	38.0
72-73	36.959929914128004	38.0	38.0	38.0	36.0	38.0
74-75	36.978893067707105	38.0	38.0	38.0	36.0	38.0
76-77	36.91335047011722	38.0	38.0	38.0	35.5	38.0
78-79	36.93555543249572	38.0	38.0	38.0	36.0	38.0
80-81	36.91016013791686	38.0	38.0	38.0	35.5	38.0
82-83	36.859810962441685	38.0	38.0	38.0	35.0	38.0
84-85	36.883117369847554	38.0	38.0	38.0	35.0	38.0
86-87	36.90270333253171	38.0	38.0	38.0	35.0	38.0
88-89	36.814354475397565	38.0	38.0	38.0	35.0	38.0
90-91	36.77101290665516	38.0	38.0	38.0	35.0	38.0
92-93	36.821341616439064	38.0	38.0	38.0	35.0	38.0
94-95	36.80213786663461	38.0	38.0	38.0	35.0	38.0
96-97	36.75288834244262	38.0	38.0	38.0	35.0	38.0
98-99	36.767511902455034	38.0	38.0	38.0	35.0	38.0
100	35.10573824884972	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	11.0
19	37.0
20	131.0
21	344.0
22	898.0
23	2292.0
24	4918.0
25	9216.0
26	16465.0
27	26041.0
28	39982.0
29	57895.0
30	79774.0
31	105656.0
32	139419.0
33	190614.0
34	287399.0
35	536930.0
36	1796097.0
37	6176023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.473609160242795	14.126620276654775	24.616399627376232	23.7833709357262
2	14.309188069995592	21.694781882293384	41.49677211707241	22.49925793063861
3	18.077616998773617	26.684393961568897	30.621135353619827	24.616853686037654
4	11.879143023922783	18.18166074911701	39.93155958900696	30.007636637953244
5	12.6072948796876	38.10998602946379	36.44223346951878	12.840485621329831
6	29.089829330460017	38.884727258827084	19.25812086317018	12.767322547542722
7	25.733054491831425	32.918242453777054	24.176730321162264	17.17197273322925
8	24.153861684439367	37.02939195631914	21.772503516842725	17.044242842398774
9	25.67990004796127	16.442139938345168	21.49123001534718	36.38672999834638
10-11	23.791311682549214	26.982963930213504	29.658520431900598	19.56720395533668
12-13	24.694703627464087	24.594335544282227	29.635986450889547	21.074974377364143
14-15	21.751051884966454	25.812358463051556	27.261264931402295	25.175324720579688
16-17	21.370561286198242	28.907945625313747	27.882865959137675	21.83862712935033
18-19	21.36176521957115	28.27864143958982	29.506833160474255	20.852760180364772
20-21	22.21574563836396	27.480125951252653	29.295846795564064	21.008281614819325
22-23	21.88126901262797	27.423296615845942	29.152233340616217	21.543201030909874
24-25	21.62081859527147	27.4620545289456	29.311420360184957	21.60570651559797
26-27	21.61686415029935	27.64908331488613	29.162105912849224	21.571946621965292
28-29	21.5476653823935	27.730459206894757	29.150903787697263	21.57097162301448
30-31	21.842450297244884	27.55398329294716	29.129099593229927	21.474466816578026
32-33	21.6208673665515	27.688263271994725	29.107891891662767	21.582977469791008
34-35	21.7033720254385	27.723630623161217	29.16502624401765	21.407971107382636
36-37	21.67877027367062	27.663189095201734	29.167237776011145	21.4908028551165
38-39	21.51939934604942	27.789502573384826	29.185480124745194	21.505617955820554
40-41	21.737802128067663	27.639122276258384	29.110885411495897	21.512190184178056
42-43	21.650390782822065	27.712967292408546	28.960535051395997	21.676106873373392
44-45	21.740148060486593	27.86641049425792	28.849974348941586	21.543467096313897
46-47	21.7212428311705	27.785718321045707	28.70417951329508	21.788859334488716
48-49	21.70929747170038	27.82968277937185	28.7390083033361	21.722011445591676
50-51	21.649267381056212	27.956186125869426	28.661445481972393	21.733101011101972
52-53	21.801055155570147	27.887392128774174	28.526352558934892	21.785200156720784
54-55	21.759238974329016	27.844998695559664	28.564184908069173	21.83157742204215
56-57	21.79296342387297	27.92331690178566	28.531729666978173	21.751990007363194
58-59	21.7670442934207	27.945775622428613	28.567591860767845	21.719588223382843
60-61	21.813573530477544	27.936729711626956	28.59441610639316	21.655280651502334
62-63	21.79330716801906	27.86488326140803	28.558490267387203	21.783319303185706
64-65	21.693797008380113	27.87937464808624	28.61979550532283	21.807032838210816
66-67	21.807720048115673	27.91153662769168	28.437819934119286	21.84292339007336
68-69	21.794197385496616	27.920178178706212	28.514691823339767	21.770932612457404
70-71	21.807980967542527	27.693572841060554	28.537961558209602	21.960484633187317
72-73	21.821850682822145	27.74971482812665	28.542944794841524	21.885489694209685
74-75	21.686229980561343	27.91387231226358	28.507948847543652	21.89194885963143
76-77	21.824037919309344	27.83750305731169	28.392897731462014	21.945561291916952
78-79	21.81147711245731	27.882462060638126	28.353347097846292	21.952713729058264
80-81	21.82054863310273	27.90203408621741	28.311735306482237	21.965681974197622
82-83	21.910188306335744	27.843884027167455	28.387799794342587	21.858127872154213
84-85	21.98904645864226	27.88475176917335	28.29003902685214	21.83616274533225
86-87	21.776904246997407	27.942479527988297	28.35551251683644	21.925103708177858
88-89	21.896552621579556	27.862796541258106	28.305749019688964	21.934901817473378
90-91	21.986043718600314	27.917579504177333	28.289909219514186	21.806467557708167
92-93	21.976208350687	27.906406463760224	28.239085368694123	21.87829981685865
94-95	21.82363295996128	28.048060080273025	28.26666877301974	21.861638186745953
96-97	21.940229638929377	28.09933426847808	28.35027132427161	21.61016476832093
98-99	22.952697788693268	30.0411753584501	28.56873423608463	18.437392616772
100	21.78593647932341	30.950581966817577	22.629439401707216	24.6340421521518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1514.0
1	1942.0
2	3172.5
3	4137.0
4	4765.5
5	5799.5
6	6906.0
7	9774.0
8	12527.0
9	12938.5
10	12559.0
11	12402.0
12	13400.0
13	16015.5
14	21212.0
15	26770.5
16	29706.5
17	30283.0
18	29404.5
19	28172.5
20	27630.0
21	28592.5
22	32586.0
23	40427.0
24	52216.0
25	68258.5
26	88662.0
27	111460.5
28	133707.0
29	157697.5
30	181482.0
31	204397.5
32	227944.0
33	249545.5
34	271568.0
35	292802.5
36	313022.0
37	331912.0
38	344219.0
39	352279.5
40	363323.5
41	372098.5
42	377649.0
43	385293.5
44	390449.5
45	394617.0
46	396619.5
47	395768.0
48	392009.0
49	382501.5
50	370691.0
51	356303.0
52	338970.0
53	321426.0
54	302814.0
55	279378.5
56	254511.5
57	229137.5
58	201684.0
59	174152.0
60	146012.5
61	118230.0
62	93596.5
63	72447.5
64	54418.5
65	39907.5
66	28668.0
67	21250.0
68	15908.0
69	11431.5
70	8191.5
71	5786.5
72	3935.5
73	2603.0
74	1659.0
75	1024.5
76	671.0
77	431.5
78	275.5
79	185.0
80	126.0
81	76.5
82	45.0
83	32.0
84	25.0
85	16.0
86	9.0
87	5.0
88	2.5
89	2.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.167851126202754E-5
3	0.0
4	6.335702252405507E-5
5	0.002238614795849946
6	2.95666105112257E-4
7	7.391652627806425E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	8.768816903827948E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.4414069301758665E-6
44-45	5.48392453330157E-6
46-47	1.6599793476436094E-5
48-49	1.1167290024795849E-4
50-51	4.901209641073714E-4
52-53	2.1018764757871214E-4
54-55	3.0928456809183275E-4
56-57	4.4492159556973657E-4
58-59	3.615909675976001E-4
60-61	3.532402046932789E-5
62-63	3.8620387084415666E-4
64-65	8.99172006449581E-5
66-67	1.028628734429057E-4
68-69	5.011037114796383E-4
70-71	8.643924993946165E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0010613008736601753
90-91	0.0
92-93	2.0734543313735275E-5
94-95	6.99010487743655E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	88.0
20-21	278.0
22-23	428.0
24-25	588.0
26-27	2094.0
28-29	6837.0
30-31	18096.0
32-33	22826.0
34-35	31822.0
36-37	53438.0
38-39	66286.0
40-41	62542.0
42-43	67916.0
44-45	79635.0
46-47	82290.0
48-49	80518.0
50-51	75295.0
52-53	70935.0
54-55	75359.0
56-57	79757.0
58-59	79982.0
60-61	79214.0
62-63	74403.0
64-65	76256.0
66-67	80778.0
68-69	83248.0
70-71	82845.0
72-73	76089.0
74-75	74642.0
76-77	78314.0
78-79	81873.0
80-81	82947.0
82-83	77016.0
84-85	73524.0
86-87	75550.0
88-89	80110.0
90-91	82138.0
92-93	80357.0
94-95	90653.0
96-97	280066.0
98-99	627671.0
100-101	6175438.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.4246139909958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.80471147099578	76.71532811982567
2	7.108851343193347	12.145417638233273
3	1.7545764604582774	4.496520505570082
4	0.6443545924813615	2.2017496934418284
5	0.2761140614742867	1.1793468559463511
6	0.14772674669171842	0.7571700187371397
7	0.07998834148960067	0.47830812368703635
8	0.05269282734772624	0.3601011549018961
9	0.0336905975136373	0.25902056589556227
>10	0.09489219510172249	1.205829040411241
>50	0.00200146246738903	0.11759592146038571
>100	3.6130740063471415E-4	0.050363885142348755
>500	1.9296702209786463E-5	0.013582925901578483
>1k	1.9296682318386178E-5	0.019665550845575308
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.055950375400918E-5	0.0	1.055950375400918E-5	0.0
5	0.0	1.055950375400918E-5	0.0	2.111900750801836E-5	0.0
6	0.0	1.055950375400918E-5	0.0	2.111900750801836E-5	0.0
7	0.0	1.055950375400918E-5	0.0	4.223801501603672E-5	0.0
8	0.0	3.167851126202754E-5	0.0	4.223801501603672E-5	0.0
9	0.0	6.335702252405507E-5	0.0	9.503553378608261E-5	0.0
10-11	0.0	6.335702252405507E-5	0.0	1.1615454129410097E-4	0.0
12-13	0.0	6.335702252405507E-5	0.0	1.3199379692511475E-4	0.0
14-15	0.0	1.1615454129410097E-4	0.0	1.4783305255612852E-4	0.0
16-17	0.0	1.1615454129410097E-4	0.0	1.5311280443313312E-4	0.0
18-19	0.0	1.2671404504811015E-4	0.0	1.6367230818714227E-4	0.0
20-21	0.0	1.3727354880211935E-4	0.0	1.7951156381815605E-4	0.0
22-23	0.0	1.583925563101377E-4	0.0	2.111900750801836E-4	0.0
24-25	0.0	1.7423181194115147E-4	0.0	2.3230908258820194E-4	0.0
26-27	0.0	1.9535081944916982E-4	0.0	2.639875938502295E-4	0.0
28-29	0.0	2.0063057132617442E-4	0.0	6.335702252405508E-4	0.0
30-31	0.0	2.111900750801836E-4	0.0	0.0014466520142992576	0.0
32-33	0.0	2.1646982695718817E-4	0.0	0.003167851126202754	0.0
34-35	0.0	2.9566610511225704E-4	0.0	0.0055279002152238054	0.0
36-37	0.0	3.695826313903213E-4	0.0	0.008727429852688586	0.0
38-39	0.0	3.695826313903213E-4	0.0	0.01428700857917442	0.0
40-41	0.0	3.9070163889833964E-4	0.0	0.020284806711451636	0.0
42-43	0.0	4.0126114265234884E-4	0.0	0.026045016009263643	0.0
44-45	0.0	4.1710039828336256E-4	0.0	0.03250743230671726	0.0
46-47	0.0	4.3293965391437634E-4	0.0	0.03937638949870023	0.0
48-49	0.0	4.3821940579138097E-4	0.0	0.04690003592343177	0.0
50-51	0.0	5.068561801924406E-4	0.0	0.05471934845327557	0.0
52-53	0.0	5.068561801924406E-4	0.0	0.06173085894593766	0.0
54-55	0.0	5.068561801924406E-4	0.0	0.06862621489730567	0.0
56-57	0.0	5.068561801924406E-4	0.0	0.07509919069851329	0.0
58-59	0.0	5.068561801924406E-4	0.0	0.08173583880790805	0.0
60-61	0.0	5.174156839464498E-4	0.0	0.08824049312037771	0.0
62-63	5.27975187700459E-6	5.27975187700459E-4	0.0	0.09473458792909337	0.0
64-65	1.055950375400918E-5	5.807727064705048E-4	0.0	0.10078518358014062	0.0
66-67	1.055950375400918E-5	5.91332210224514E-4	0.0	0.10690969575746594	0.0
68-69	1.055950375400918E-5	6.124512177325325E-4	0.0	0.11268574431090897	5.27975187700459E-6
70-71	1.055950375400918E-5	6.282904733635462E-4	0.0	0.11798133544354455	1.055950375400918E-5
72-73	1.055950375400918E-5	6.335702252405508E-4	0.0	0.12213650017074718	1.055950375400918E-5
74-75	1.583925563101377E-5	6.335702252405508E-4	0.0	0.1251776372519018	1.055950375400918E-5
76-77	2.111900750801836E-5	6.441297289945599E-4	0.0	0.1283824466412436	1.055950375400918E-5
78-79	2.111900750801836E-5	6.546892327485691E-4	0.0	0.13056298416644652	1.055950375400918E-5
80-81	2.111900750801836E-5	6.546892327485691E-4	0.0	0.13113847712104	1.055950375400918E-5
82-83	2.111900750801836E-5	6.546892327485691E-4	0.0	0.13172452957938752	1.055950375400918E-5
84-85	2.639875938502295E-5	6.546892327485691E-4	0.0	0.13203075518825377	1.055950375400918E-5
86-87	3.167851126202754E-5	6.652487365025783E-4	0.0	0.13212051097016286	1.055950375400918E-5
88	3.167851126202754E-5	7.07486751518615E-4	0.0	0.13213107047391687	1.055950375400918E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9315	0.0	46.579323	1
GTATCAA	17340	0.0	33.951805	1
ATCAACG	21625	0.0	30.45024	3
TCAACGC	22190	0.0	29.713259	4
CAACGCA	22490	0.0	29.335817	5
TATCAAC	23290	0.0	28.474262	2
AACGCAG	23220	0.0	28.412798	6
ACGCAGA	26095	0.0	25.217234	7
TTCAACG	1495	0.0	24.18542	1
CGCAGAG	27335	0.0	24.04218	8
TACATGG	27415	0.0	22.35896	2
ACATGGG	27595	0.0	21.658175	3
GTACATG	27485	0.0	20.212654	1
GCAGAGT	32855	0.0	20.002829	9
CATGGGG	19210	0.0	18.888502	4
AGAGTAC	29965	0.0	18.148922	10-11
GAGTACT	19145	0.0	18.074512	12-13
TCGCAGA	3425	0.0	17.88454	1
CAGAGTA	32500	0.0	17.688747	10-11
GTACTTT	21565	0.0	15.997152	14-15
>>END_MODULE