FastQCFastQC Report
Fri 27 May 2016
SRR2140029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10449938
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102600.045.6140141
GTATCAA174950.033.4803241
ATCAACG195050.028.9257763
TCAACGC195650.028.8155024
CAACGCA200700.028.0905825
AACGCAG210450.026.9297876
TATCAAC217600.026.4334052
ACGCAGA245300.023.069447
CGCAGAG249450.022.6683988
GTACATG261500.022.0118621
TACATGG261400.021.5522122
ACATGGG263850.020.7115333
GCAGAGT309000.018.3544069
CATGGGG191350.018.348244
GAGTACT175250.018.1315312-13
CAGAGTA302150.016.92706510-11
AGAGTAC271150.016.72239310-11
GTACTTT206150.015.48609114-15
GAGTACA187400.014.4345261
ATGGGGG125450.014.2289015
Produced by FastQC (version 0.11.5)