FastQCFastQC Report
Fri 27 May 2016
SRR2140028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11909787
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110750.043.1140441
CTTATAC110900.035.929781
GTATCAA176650.031.0093571
TATACAC168550.026.7239253
ATCAACG198600.026.6384183
TCAACGC198750.026.576464
CAACGCA203500.025.9151425
AACGCAG210900.025.1833236
TTATACA169100.024.7934822
TATCAAC222400.024.3685822
TACATGG276050.022.0734632
ACACATC189600.021.870346
GTACATG289800.021.8442381
ACATCTC190150.021.7191358
ACGCAGA248150.021.3527537
ACATGGG284100.020.9931953
CGCAGAG253950.020.8810128
TACACAT222900.020.226375
CACATCT215450.019.0532657
CATGGGG208150.018.9822544
Produced by FastQC (version 0.11.5)