##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11909787
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40716252943902	33.0	31.0	34.0	31.0	34.0
2	32.5981315199004	34.0	31.0	34.0	31.0	34.0
3	32.69560614308216	34.0	31.0	34.0	31.0	34.0
4	36.18240620088336	37.0	35.0	37.0	35.0	37.0
5	35.98240262399319	37.0	35.0	37.0	35.0	37.0
6	36.04512792714093	37.0	35.0	37.0	35.0	37.0
7	36.032453812985906	37.0	35.0	37.0	35.0	37.0
8	36.03187000741491	37.0	35.0	37.0	35.0	37.0
9	37.911903462253356	39.0	38.0	39.0	35.0	39.0
10-11	37.755738284824076	39.0	37.5	39.0	35.0	39.0
12-13	37.76171454619633	39.0	37.5	39.0	35.0	39.0
14-15	39.11892328552979	40.0	39.0	41.0	36.0	41.0
16-17	39.07428264670057	40.0	38.0	41.0	36.0	41.0
18-19	39.10234293023041	40.0	38.5	41.0	36.0	41.0
20-21	39.111776927047984	40.0	39.0	41.0	36.0	41.0
22-23	39.0432009353444	40.0	39.0	41.0	36.0	41.0
24-25	39.06511259265772	40.0	39.0	41.0	36.0	41.0
26-27	38.95868035441687	40.0	38.5	41.0	35.5	41.0
28-29	38.880649938775356	40.0	38.0	41.0	35.0	41.0
30-31	38.77997933635149	40.0	38.0	41.0	35.0	41.0
32-33	38.71444804456944	40.0	38.0	41.0	35.0	41.0
34-35	38.65980285979287	40.0	38.0	41.0	35.0	41.0
36-37	38.64494605983622	40.0	38.0	41.0	35.0	41.0
38-39	38.55639638500266	40.0	38.0	41.0	35.0	41.0
40-41	38.54487787542409	40.0	38.0	41.0	35.0	41.0
42-43	38.50185044631019	40.0	38.0	41.0	34.0	41.0
44-45	38.41425385561453	40.0	38.0	41.0	34.0	41.0
46-47	38.35137515300029	40.0	38.0	41.0	34.0	41.0
48-49	38.35963860640669	40.0	38.0	41.0	34.0	41.0
50-51	38.282380645072834	40.0	38.0	41.0	34.0	41.0
52-53	38.11146254655016	40.0	37.0	41.0	33.0	41.0
54-55	37.945781135423104	40.0	37.0	41.0	33.0	41.0
56-57	37.816181822280726	39.5	37.0	41.0	33.0	41.0
58-59	37.661876383458164	39.0	36.0	41.0	33.0	41.0
60-61	37.47396046948024	39.0	36.0	41.0	32.5	41.0
62-63	37.32057953648992	39.0	35.5	40.5	32.0	41.0
64-65	37.1260381058414	39.0	35.0	40.0	32.0	41.0
66-67	36.850772684460516	38.0	35.0	40.0	32.0	41.0
68-69	36.611605905141445	37.5	35.0	40.0	32.0	41.0
70-71	36.32140768612034	37.0	35.0	39.5	31.0	41.0
72-73	36.015149285324384	36.5	35.0	39.0	31.0	41.0
74-75	35.582598356403665	36.0	34.0	39.0	31.0	40.5
76-77	33.6546172428513	34.5	32.0	36.5	28.0	39.0
78-79	34.73282951740481	35.0	34.0	37.0	30.0	39.0
80-81	34.705586602102684	35.0	34.0	37.0	30.5	39.0
82-83	34.457784373852164	35.0	34.0	36.5	30.5	39.0
84-85	34.1893528170138	35.0	34.0	36.0	30.0	37.0
86-87	33.9533932514861	35.0	34.0	36.0	30.0	37.0
88-89	33.7978500471131	35.0	34.0	35.0	30.0	37.0
90-91	33.71088168579702	35.0	34.0	35.0	30.0	36.0
92-93	33.5907029382701	35.0	34.0	35.0	30.0	36.0
94-95	33.49198155322767	35.0	33.5	35.0	30.0	36.0
96-97	33.481692769743375	35.0	33.0	35.0	30.0	36.0
98-99	33.60392795806612	35.0	34.0	35.0	30.0	35.0
100-101	32.38170250547156	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	15.0
20	66.0
21	276.0
22	1057.0
23	3331.0
24	8912.0
25	19112.0
26	35082.0
27	56979.0
28	84538.0
29	118487.0
30	157961.0
31	205790.0
32	272678.0
33	369482.0
34	536691.0
35	828489.0
36	1479003.0
37	2776136.0
38	3441459.0
39	1487558.0
40	26676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.84648306472651	20.258960130857083	12.906460879611029	24.98809592480537
2	14.847925390450603	21.603160164621578	40.702376547042874	22.846537897884946
3	18.325989931808174	26.208407624486924	30.547789065881542	24.91781337782336
4	12.248122209847667	18.133697919955292	38.94144156247187	30.67673830772517
5	12.82742504127068	38.00812726541625	36.31986869286579	12.844579000447279
6	29.736887821755335	38.452157036897475	18.837608094922267	12.973347046424927
7	26.200432997557588	32.262222474494386	23.57742568644152	17.959918841506504
8	24.22339893333593	37.111078122000855	21.716054891267202	16.94946805339601
9	25.395718796891597	16.13987425295658	21.058998304167254	37.405408645984565
10-11	23.988272990795835	26.707456142210624	29.52318186793088	19.78108899906266
12-13	24.722766511515356	24.094610043526025	29.4005682644709	21.78205518048772
14-15	21.730347922346873	25.65857617568883	26.535943053207344	26.075132848756954
16-17	21.80765506766329	28.863436155158496	27.228879679156847	22.100029098021373
18-19	21.81312730445977	28.156460732673054	28.58694282273898	21.443469140128197
20-21	22.37759298853666	27.46815629586009	28.382988152923673	21.77126256267957
22-23	22.097399114481743	27.660159547134917	28.24620676027511	21.996234578108233
24-25	21.961673903738856	27.73659837573582	28.430251049945603	21.87147667057972
26-27	22.07507189217447	27.92226846921538	28.262550952247263	21.740108686362895
28-29	21.84760058127769	27.795349080496518	28.433499850164708	21.92355048806109
30-31	22.241511138373355	27.7815103314005	28.25505514160057	21.721923388625576
32-33	21.966191607866175	27.73776784625846	28.333633661261015	21.96240688461435
34-35	21.839057457783838	28.00042686033497	28.553081257070616	21.607434424810574
36-37	22.067340640076498	27.868730153350374	28.248609175784107	21.815320030789017
38-39	21.784024715548696	27.772705983252216	28.465840568342077	21.97742873285701
40-41	21.98773942398678	27.64802914198434	28.423960670534186	21.940270763494695
42-43	22.01176674687493	27.693845811555722	28.34015712477114	21.95423031679821
44-45	21.91036028918602	27.949415650209996	28.190642668749856	21.94958139185413
46-47	22.160766528061238	27.750310103954295	27.818762564614584	22.270160803369883
48-49	22.053605784146637	27.78196620390864	27.902217501034315	22.2622105109104
50-51	21.858475521618185	27.890269644553218	28.055437838834397	22.1958169949942
52-53	22.03179830796494	27.864412141222523	28.116563215867163	21.987226334945372
54-55	21.97209438986456	27.856030195228946	27.949151253865207	22.22272416104128
56-57	22.194335948147454	28.048283918791693	27.647511684961902	22.109868448098954
58-59	21.93472986673267	28.236844932756732	27.94521053176459	21.883214668746
60-61	22.197338759004477	28.401063350783794	27.53326043265038	21.86833745756135
62-63	21.957325163720533	28.56728446081472	27.569464919393376	21.905925456071373
64-65	21.985244437575922	28.380748205225288	27.608247787749313	22.025759569449477
66-67	22.099632525893238	28.231382783483117	27.548847548023947	22.120137142599706
68-69	22.120302538853075	28.11454695048292	27.641902607503503	22.123247903160497
70-71	22.093677544935364	27.93248045890293	27.72207082218905	22.251771173972656
72-73	22.079959795408687	27.98082029567214	27.730137022361557	22.209082886557617
74-75	22.019543282120267	28.062942401297324	27.708168108807875	22.209346207774527
76-77	22.121680611617382	28.046742709432674	27.614481840137024	22.21709483881292
78-79	22.12240961936838	28.00957120619765	27.582856642751814	22.28516253168216
80-81	22.051374712065325	28.05229930260145	27.645974743929617	22.2503512414036
82-83	22.12590772960481	28.00683558411054	27.73793560475716	22.129321081527493
84-85	22.224365622627133	27.969903062047585	27.66767611273152	22.138055202593762
86-87	22.086648596883805	27.996813637685324	27.733981984036216	22.182555781394655
88-89	22.12318641568013	28.01152595542132	27.671637788365068	22.19364984053348
90-91	22.150365272772213	28.024021169455427	27.69541546436627	22.130198093406094
92-93	22.15210772460213	27.990894922917448	27.7007791445003	22.156218207980125
94-95	22.032130273000273	28.05730966759811	27.770704574928285	22.13985548447334
96-97	22.050528282785894	28.07759043042251	27.77411995839849	22.097761328393105
98-99	22.299091926582516	28.6362452123573	27.839801267516478	21.224861593543704
100-101	26.294841170477596	35.678175298720326	28.44458255995273	9.582400970849347
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1893.0
1	1732.5
2	2431.0
3	3537.0
4	4873.5
5	7831.0
6	10353.0
7	11970.5
8	12813.5
9	12403.0
10	11211.5
11	10161.0
12	11107.0
13	14615.0
14	22361.0
15	32499.5
16	40505.0
17	45458.5
18	47390.0
19	47389.0
20	46757.5
21	46652.0
22	49179.0
23	56701.0
24	70057.5
25	88970.5
26	112858.0
27	138787.0
28	165218.5
29	191421.0
30	215232.0
31	238926.0
32	264629.5
33	292391.0
34	321237.0
35	348445.0
36	375700.5
37	401070.0
38	421981.0
39	439883.5
40	457160.5
41	474679.5
42	488107.5
43	498289.0
44	508526.5
45	520699.0
46	536857.5
47	553008.0
48	549642.0
49	532686.5
50	518988.5
51	498342.0
52	469086.0
53	439145.5
54	408122.0
55	373863.0
56	341069.0
57	307230.0
58	270696.5
59	235164.0
60	198834.5
61	162936.5
62	131010.5
63	103124.5
64	78856.0
65	59738.0
66	45373.0
67	34746.5
68	26586.0
69	19303.5
70	13808.5
71	9949.0
72	7102.5
73	4936.0
74	3335.0
75	2256.5
76	1542.0
77	1030.5
78	715.5
79	514.0
80	373.5
81	252.0
82	180.0
83	124.0
84	83.0
85	65.0
86	52.0
87	44.5
88	32.5
89	21.5
90	15.0
91	8.0
92	3.5
93	2.0
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004483707391240498
3	1.6792911577679767E-5
4	3.6104759892011503E-4
5	0.0
6	0.0
7	0.002107510402998811
8	0.0011167286199157047
9	3.358582315535953E-5
10-11	1.301450647270182E-4
12-13	1.301450647270182E-4
14-15	2.5189367366519653E-5
16-17	3.568493710256951E-4
18-19	0.0
20-21	9.656404553641307E-5
22-23	0.0
24-25	0.0
26-27	7.058706675192016E-4
28-29	2.0182628399674407E-4
30-31	2.1058786064592606E-5
32-33	0.0018669826401026858
34-35	4.875715055411017E-4
36-37	0.001291740813868334
38-39	0.003980285636644968
40-41	9.107729213678993E-5
42-43	8.663204914154858E-4
44-45	0.0018518523429291106
46-47	9.38929016429827E-5
48-49	2.57787290480553E-4
50-51	2.194799824196534E-4
52-53	0.0
54-55	1.7267080385912714E-4
56-57	0.0016896990424173457
58-59	0.0032963289357916684
60-61	0.0011804333496610486
62-63	0.0010482628545559292
64-65	4.959619275818216E-6
66-67	1.1061821200141228E-4
68-69	1.0191570959322383E-5
70-71	4.12830823270324E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	2.2200657983101304E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	403.0
20-21	1159.0
22-23	2096.0
24-25	4520.0
26-27	7107.0
28-29	15359.0
30-31	32539.0
32-33	41147.0
34-35	56550.0
36-37	90009.0
38-39	105258.0
40-41	100220.0
42-43	109451.0
44-45	127993.0
46-47	129218.0
48-49	123115.0
50-51	113030.0
52-53	107884.0
54-55	118002.0
56-57	126169.0
58-59	130374.0
60-61	130354.0
62-63	125475.0
64-65	131282.0
66-67	137504.0
68-69	124323.0
70-71	118455.0
72-73	110049.0
74-75	109364.0
76-77	108485.0
78-79	116983.0
80-81	118711.0
82-83	111977.0
84-85	108641.0
86-87	113178.0
88-89	122838.0
90-91	130226.0
92-93	134621.0
94-95	145910.0
96-97	222513.0
98-99	961563.0
100-101	6985732.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.9863016939356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4522361904576	76.02214732079412
2	7.33495750670458	12.46741823153986
3	1.8247145881365314	4.652272334780901
4	0.6517809093954959	2.215697960169333
5	0.2993436874946307	1.272005646779693
6	0.15509660650870588	0.7908652195472696
7	0.09083426413068782	0.5403767722890087
8	0.05486733883595134	0.37303777691644385
9	0.034418288641576346	0.2632574756053767
>10	0.10013006500827479	1.2631660404319847
>50	0.0012205552950922752	0.06695879870759068
>100	3.6900579784358677E-4	0.05303151192068722
>500	3.099359308922906E-5	0.019764910517708945
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.396455788839885E-6	0.0
2	0.0	0.0	0.0	8.396455788839885E-6	0.0
3	0.0	0.0	0.0	1.679291157767977E-5	0.0
4	0.0	5.0378734733039306E-5	0.0	4.198227894419942E-5	0.0
5	0.0	6.717164631071908E-5	0.0	5.0378734733039306E-5	0.0
6	8.396455788839885E-6	6.717164631071908E-5	0.0	5.0378734733039306E-5	0.0
7	8.396455788839885E-6	6.717164631071908E-5	0.0	6.717164631071908E-5	0.0
8	8.396455788839885E-6	6.717164631071908E-5	0.0	9.236101367723872E-5	8.396455788839885E-6
9	8.396455788839885E-6	1.3434329262143816E-4	0.0	1.4273974841027802E-4	1.679291157767977E-5
10-11	4.198227894419942E-5	1.3434329262143816E-4	0.0	1.511362041991179E-4	1.679291157767977E-5
12-13	5.0378734733039306E-5	1.679291157767977E-4	0.0	1.511362041991179E-4	1.679291157767977E-5
14-15	5.4576962627459245E-5	2.0991139472099712E-4	0.0	1.6373088788237776E-4	2.9387595260939595E-5
16-17	5.877519052187919E-5	2.2670430629867687E-4	0.0	1.7632557156563758E-4	3.358582315535954E-5
18-19	5.877519052187919E-5	2.4349721787635662E-4	0.0	1.7632557156563758E-4	4.198227894419942E-5
20-21	6.297341841629913E-5	2.7288481313729625E-4	0.0	2.0991139472099712E-4	5.4576962627459245E-5
22-23	8.396455788839885E-5	2.980741805038159E-4	0.0	2.644883573484564E-4	6.297341841629913E-5
24-25	1.0495569736049856E-4	3.2326354787033554E-4	0.0	3.022724083982358E-4	7.136987420513902E-5
26-27	1.091539252549185E-4	3.358582315535954E-4	0.0	3.316600036591754E-4	7.556810209955896E-5
28-29	1.091539252549185E-4	3.4425468734243525E-4	0.0	7.178969699458101E-4	7.556810209955896E-5
30-31	1.091539252549185E-4	3.736422826033748E-4	0.0	0.0017884450830228955	7.97663299939789E-5
32-33	1.1755038104375838E-4	3.778405104977948E-4	0.0	0.003576890166045791	8.396455788839885E-5
34-35	1.301450647270182E-4	4.1982278944199423E-4	0.0	0.006133610953747535	9.236101367723872E-5
36-37	1.4273974841027802E-4	4.660032962806136E-4	0.0	0.009886826691358964	9.236101367723872E-5
38-39	1.4273974841027802E-4	4.8699443575271335E-4	0.0	0.016242943723510754	9.655924157165867E-5
40-41	1.679291157767977E-4	5.331749425913327E-4	0.0	0.02308185696352084	1.0075746946607861E-4
42-43	1.679291157767977E-4	5.919501331132118E-4	0.0	0.028694887658360306	1.0915392525491849E-4
44-45	1.8052379946005751E-4	6.50725323635091E-4	0.0	0.035462431024165256	1.2174860893817832E-4
46-47	2.0571316682657718E-4	6.885093746848705E-4	0.0	0.043241747312525405	1.301450647270182E-4
48-49	2.18307850509837E-4	6.927076025792904E-4	0.0	0.050332554226200685	1.4273974841027802E-4
50-51	2.18307850509837E-4	7.724739325732693E-4	0.0	0.05707910645253353	1.4273974841027802E-4
52-53	2.2670430629867687E-4	8.144562115174688E-4	0.0	0.06377947817202775	1.511362041991179E-4
54-55	2.3090253419309683E-4	8.228526673063086E-4	0.0	0.07040428178942243	1.595326599879578E-4
56-57	2.3510076208751676E-4	8.228526673063086E-4	0.0	0.0766512448963193	1.595326599879578E-4
58-59	2.4349721787635665E-4	8.270508952007286E-4	0.0	0.08344397762949077	1.595326599879578E-4
60-61	2.476954457707766E-4	8.312491230951486E-4	0.0	0.08988405921953097	1.7632557156563758E-4
62-63	2.5189367366519654E-4	8.438438067784083E-4	0.0	0.09577417295540214	1.7632557156563758E-4
64-65	2.5189367366519654E-4	8.900243136170277E-4	0.0	0.10161390795654028	1.7632557156563758E-4
66-67	2.5189367366519654E-4	9.152136809835474E-4	0.0	0.10760477916187755	1.7632557156563758E-4
68-69	2.602901294540364E-4	9.362048204556472E-4	0.0	0.11362503796247572	1.7632557156563758E-4
70-71	2.602901294540364E-4	9.529977320333269E-4	0.0	0.11969567549780698	1.7632557156563758E-4
72-73	2.686865852428763E-4	9.571959599277469E-4	0.0	0.12459080922270063	2.18307850509837E-4
74-75	2.770830410317162E-4	9.781870993998466E-4	0.0	0.12895696623289737	2.2250607840425694E-4
76-77	2.770830410317162E-4	9.991782388719462E-4	0.0	0.13253385639894316	2.2670430629867687E-4
78-79	2.812812689261361E-4	9.991782388719462E-4	0.0	0.13607716074183357	2.2670430629867687E-4
80-81	2.9387595260939596E-4	9.991782388719462E-4	0.0	0.13773546076012946	2.392989899819367E-4
82-83	2.980741805038159E-4	9.991782388719462E-4	0.0	0.13831481620955943	2.5189367366519654E-4
84-85	3.022724083982358E-4	0.0010033764667663663	0.0	0.13880600887320654	2.5189367366519654E-4
86-87	3.022724083982358E-4	0.001015971150449626	0.0	0.1390495060910829	2.7288481313729625E-4
88-89	3.064706362926558E-4	0.0010579534293938253	0.0	0.13909568659792151	2.770830410317162E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11075	0.0	43.114044	1
CTTATAC	11090	0.0	35.92978	1
GTATCAA	17665	0.0	31.009357	1
TATACAC	16855	0.0	26.723925	3
ATCAACG	19860	0.0	26.638418	3
TCAACGC	19875	0.0	26.57646	4
CAACGCA	20350	0.0	25.915142	5
AACGCAG	21090	0.0	25.183323	6
TTATACA	16910	0.0	24.793482	2
TATCAAC	22240	0.0	24.368582	2
TACATGG	27605	0.0	22.073463	2
ACACATC	18960	0.0	21.87034	6
GTACATG	28980	0.0	21.844238	1
ACATCTC	19015	0.0	21.719135	8
ACGCAGA	24815	0.0	21.352753	7
ACATGGG	28410	0.0	20.993195	3
CGCAGAG	25395	0.0	20.881012	8
TACACAT	22290	0.0	20.22637	5
CACATCT	21545	0.0	19.053265	7
CATGGGG	20815	0.0	18.982254	4
>>END_MODULE