FastQCFastQC Report
Fri 27 May 2016
SRR2140027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11413259
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120750.047.9548341
GTATCAA215050.035.9150961
TCAACGC253900.029.5208284
ATCAACG259600.028.953933
CAACGCA260100.028.8494665
AACGCAG270700.027.7665586
TATCAAC274650.027.568552
ACGCAGA318250.023.5914127
CGCAGAG324650.023.0868368
GTACATG336000.023.0118541
TACATGG335600.022.5868422
ACATGGG340150.021.7003943
GCAGAGT386700.019.4257139
CATGGGG256200.019.2732524
GAGTACT221050.017.82201812-13
AGAGTAC346200.017.79135910-11
CAGAGTA381550.017.24355310-11
GTGGTAT64150.017.2373311
ATGGGGG161650.016.4740375
TGGTATC59100.016.425232
Produced by FastQC (version 0.11.5)