##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140027_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11413259
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.423094490364235	33.0	31.0	34.0	31.0	34.0
2	32.60435297227549	34.0	31.0	34.0	31.0	34.0
3	32.69615409586341	34.0	31.0	34.0	31.0	34.0
4	36.18607086722557	37.0	35.0	37.0	35.0	37.0
5	35.98912116162439	37.0	35.0	37.0	35.0	37.0
6	36.05784123535618	37.0	35.0	37.0	35.0	37.0
7	36.04039792665706	37.0	35.0	37.0	35.0	37.0
8	36.04333197029875	37.0	35.0	37.0	35.0	37.0
9	37.9170549796513	39.0	38.0	39.0	35.0	39.0
10-11	37.761958569414745	39.0	37.5	39.0	35.0	39.0
12-13	37.76628340774532	39.0	38.0	39.0	35.0	39.0
14-15	39.11574989229632	40.0	38.5	41.0	36.0	41.0
16-17	39.07679839737274	40.0	38.0	41.0	36.0	41.0
18-19	39.11352502383412	40.0	38.5	41.0	36.0	41.0
20-21	39.120362581275344	40.0	39.0	41.0	36.0	41.0
22-23	39.056609143157544	40.0	39.0	41.0	36.0	41.0
24-25	39.07874472075505	40.0	39.0	41.0	36.0	41.0
26-27	38.968665797133255	40.0	39.0	41.0	35.0	41.0
28-29	38.88806809775487	40.0	38.0	41.0	35.0	41.0
30-31	38.787900419715285	40.0	38.0	41.0	35.0	41.0
32-33	38.709427993906175	40.0	38.0	41.0	35.0	41.0
34-35	38.655526881957684	40.0	38.0	41.0	35.0	41.0
36-37	38.64048004246047	40.0	38.0	41.0	35.0	41.0
38-39	38.5580162941874	40.0	38.0	41.0	35.0	41.0
40-41	38.526575249746855	40.0	38.0	41.0	34.5	41.0
42-43	38.47508434191222	40.0	38.0	41.0	34.0	41.0
44-45	38.378730686445905	40.0	38.0	41.0	34.0	41.0
46-47	38.306182435913435	40.0	38.0	41.0	34.0	41.0
48-49	38.306528940967716	40.0	38.0	41.0	34.0	41.0
50-51	38.235036951885675	40.0	38.0	41.0	34.0	41.0
52-53	38.05117652298706	40.0	37.0	41.0	33.0	41.0
54-55	37.88164199155385	40.0	37.0	41.0	33.0	41.0
56-57	37.75140828843289	39.0	36.5	41.0	33.0	41.0
58-59	37.58490542298176	39.0	36.0	41.0	33.0	41.0
60-61	37.401104194092596	39.0	36.0	41.0	32.5	41.0
62-63	37.23000970372105	39.0	35.0	40.0	32.0	41.0
64-65	37.011338834753424	38.5	35.0	40.0	32.0	41.0
66-67	36.719289026144864	38.0	35.0	40.0	32.0	41.0
68-69	36.466984259205674	37.0	35.0	40.0	31.5	41.0
70-71	36.174918543627896	37.0	35.0	39.5	31.0	41.0
72-73	35.87101307943725	36.0	35.0	39.0	31.0	41.0
74-75	35.44078672651934	36.0	34.0	39.0	31.0	40.0
76-77	33.5339323365485	34.5	32.0	36.5	28.0	39.0
78-79	34.61847780227085	35.0	34.0	37.0	30.0	39.0
80-81	34.60445396049147	35.0	34.0	37.0	30.5	39.0
82-83	34.370785414592376	35.0	34.0	36.0	30.0	38.5
84-85	34.11978352178092	35.0	34.0	36.0	30.0	37.0
86-87	33.89452822249157	35.0	34.0	35.5	30.0	37.0
88-89	33.74556961669603	35.0	34.0	35.0	30.0	36.5
90-91	33.674874525500826	35.0	34.0	35.0	30.0	36.0
92-93	33.55972163325005	35.0	34.0	35.0	30.0	36.0
94-95	33.4667299657126	35.0	33.0	35.0	30.0	36.0
96-97	33.46096685758093	35.0	33.0	35.0	30.0	35.5
98-99	33.58570227268452	35.0	34.0	35.0	30.0	35.0
100-101	32.31831293425706	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	19.0
20	63.0
21	270.0
22	1022.0
23	3414.0
24	8713.0
25	18696.0
26	34159.0
27	55808.0
28	82591.0
29	115557.0
30	154196.0
31	200917.0
32	265745.0
33	363038.0
34	534060.0
35	832843.0
36	1500243.0
37	2758941.0
38	3172720.0
39	1288308.0
40	21934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49249053228355	19.929250707444737	13.36757537877656	24.210683381495155
2	15.066954017600304	21.346330133347262	40.569860501729735	23.016855347322707
3	18.480002551419155	26.144090695941628	30.270555571521395	25.10535118111782
4	12.600393491229244	17.677472999501017	38.70593868598813	31.016194823281605
5	13.115990796318563	38.01786150651624	35.71151763050326	13.154630066661941
6	30.291175403202136	37.88803337311747	18.644308224697976	13.176482998982411
7	26.650095579175105	32.481726402519364	23.350048992158186	17.518129026147346
8	24.778906446114153	36.650654442176595	21.21317900265089	17.357260109058362
9	25.91446102176128	16.219495002870783	21.00885698406931	36.857186991298626
10-11	24.394946503830152	26.58296539100573	29.05825572687597	19.963832378288142
12-13	25.089916400166985	24.18563334400953	29.139077547695592	21.5853727081279
14-15	22.17419423830374	25.53404688680577	26.57889356439862	25.71286531049188
16-17	21.898261673895565	28.6707889035006	27.237937476195373	22.19301194640847
18-19	21.948016775927016	27.977236826045914	28.77618478648386	21.298561611543207
20-21	22.68319195901278	27.208209991511232	28.630643786481198	21.47795426299479
22-23	22.427125579068857	27.097537258693105	28.515589691006856	21.959747471231182
24-25	22.160534469479945	27.242375426338754	28.603052524794514	21.994037579386784
26-27	22.118158276280834	27.366680029571732	28.513617162723047	22.00154453142439
28-29	22.06865184027996	27.476123864253786	28.50617306864293	21.94905122682332
30-31	22.326771360131502	27.35150311711011	28.40068030128432	21.921045221474067
32-33	22.090072663460628	27.525045234313765	28.36990516202257	22.014976940203038
34-35	22.217238193874756	27.54678802650466	28.35364372765778	21.882330051962807
36-37	22.194142084235054	27.490501471080186	28.309970381224993	22.00538606345977
38-39	22.037118078591476	27.616558576675732	28.392902552400567	21.953420792332224
40-41	22.288170867085505	27.475728759008927	28.245500413753504	21.990599960152064
42-43	22.138848036627845	27.572544646658404	28.1299549213559	22.15865239535785
44-45	22.237396372485847	27.697587924285326	27.951692301233223	22.1133234019956
46-47	22.304056129504822	27.66688117373822	27.739373444765818	22.289689251991142
48-49	22.2176243124732	27.769970227363828	27.72686131182485	22.285544148338122
50-51	22.214850206864405	27.900255229166394	27.633495892341315	22.251398671627886
52-53	22.42945154002321	27.75400581492419	27.517901602211413	22.298641042841176
54-55	22.342693613928013	27.665460052502382	27.601767512545926	22.390078821023675
56-57	22.317300889281018	27.814930561519365	27.57087421354607	22.29689433565355
58-59	22.33316798407691	27.84605379470643	27.519249323225935	22.301528897990718
60-61	22.37038295025621	27.802911142003968	27.57559043338106	22.25111547435876
62-63	22.358311726499704	27.85658704489493	27.518738387801427	22.26636284080394
64-65	22.3200960345593	27.834270931604475	27.49195592950997	22.353677104326255
66-67	22.41513148253851	27.838749048249035	27.372670323713177	22.37344914549928
68-69	22.340583971472817	27.863512530597635	27.4390322265708	22.35687127135875
70-71	22.347153660723524	27.63125595531262	27.51867977161379	22.502910612350053
72-73	22.37542873730758	27.71812214358525	27.45490588752746	22.451543231579716
74-75	22.233606771645345	27.849670897568707	27.440803038995483	22.475919291790465
76-77	22.382915471594565	27.751209171646245	27.378368367993815	22.487506988765375
78-79	22.348425853760308	27.776047570632816	27.317065704489846	22.558460871117024
80-81	22.323913919124607	27.839843297845846	27.323971683770555	22.51227109925899
82-83	22.346471208857512	27.804802107348404	27.42287445566156	22.425852228132523
84-85	22.442743424553573	27.769942885169886	27.35582450356686	22.431489186709687
86-87	22.315574127083693	27.77845258922656	27.439425140547414	22.466548143142337
88-89	22.34552383561033	27.778228259494515	27.41107884961486	22.465169055280295
90-91	22.368715000441412	27.830412086283808	27.40968169567309	22.391191217601698
92-93	22.37089493307233	27.764745986278694	27.43155804441163	22.432801036237347
94-95	22.267136071487386	27.788948700016896	27.490544841389504	22.453370387106215
96-97	22.29292588693786	27.82512285132771	27.505658026364582	22.37629323536985
98-99	22.547820556090045	28.430621878694772	27.515664496872933	21.505893068342246
100-101	23.2992536361326	30.983261758530578	24.642171254222216	21.0753133511146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3483.0
1	2912.0
2	3348.0
3	4636.5
4	5998.0
5	9120.5
6	11971.0
7	14374.5
8	16334.0
9	16295.0
10	14609.0
11	13039.5
12	13856.0
13	17619.5
14	26502.5
15	38399.5
16	48010.5
17	54002.0
18	55853.0
19	54135.5
20	51019.5
21	48587.0
22	49013.5
23	53933.0
24	64450.5
25	80748.5
26	101087.5
27	123578.5
28	148917.0
29	176371.0
30	201574.5
31	225342.0
32	250486.0
33	274767.0
34	297130.5
35	318157.5
36	341949.0
37	363062.0
38	380003.0
39	395583.0
40	410858.5
41	426540.0
42	436338.0
43	444770.5
44	455044.0
45	464102.5
46	469008.5
47	471332.0
48	473189.5
49	469693.0
50	463849.0
51	454267.5
52	438015.0
53	420881.5
54	402003.0
55	378263.5
56	350880.0
57	320941.5
58	288981.0
59	254990.0
60	218112.0
61	181428.5
62	147346.0
63	116415.0
64	88936.5
65	67214.0
66	50869.5
67	38737.5
68	29568.5
69	21566.0
70	15388.5
71	11018.5
72	7687.5
73	5230.5
74	3546.0
75	2419.0
76	1656.0
77	1090.5
78	725.5
79	503.5
80	364.0
81	263.0
82	182.5
83	120.5
84	86.5
85	62.5
86	45.5
87	33.0
88	22.0
89	15.5
90	12.0
91	10.0
92	7.5
93	5.5
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0043896313927511855
3	2.628521792066578E-5
4	3.855165295030981E-4
5	0.0
6	8.761739306888595E-6
7	0.0018224417758328274
8	9.112208879164137E-4
9	5.257043584133156E-5
10-11	1.0514087168266312E-4
12-13	1.6209217717743897E-4
14-15	4.818956618788726E-5
16-17	3.329460936617666E-4
18-19	0.0
20-21	9.63835237097773E-5
22-23	0.0
24-25	0.0
26-27	7.409977758666758E-4
28-29	1.5357014610400436E-4
30-31	3.075978920404343E-5
32-33	0.00186348110958802
34-35	5.922457965023113E-4
36-37	0.0015320510406578813
38-39	0.0039660513161432955
40-41	1.6670863669236366E-4
42-43	9.763576139091452E-4
44-45	0.002099158759835338
46-47	7.412765876221821E-5
48-49	3.183878487464813E-4
50-51	2.3173791709041599E-4
52-53	0.0
54-55	1.7343071339423225E-4
56-57	0.0018051251151192996
58-59	0.0034170028686107824
60-61	0.0011378079161619404
62-63	0.0011394343938020792
64-65	0.0
66-67	1.434669652965708E-4
68-69	5.182086873022548E-6
70-71	4.1943418956978323E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.6872822562248343E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	319.0
20-21	1021.0
22-23	1959.0
24-25	4986.0
26-27	6812.0
28-29	13331.0
30-31	27308.0
32-33	34235.0
34-35	47170.0
36-37	72200.0
38-39	85466.0
40-41	85729.0
42-43	95898.0
44-45	115057.0
46-47	115201.0
48-49	109107.0
50-51	98906.0
52-53	94621.0
54-55	101268.0
56-57	106277.0
58-59	106371.0
60-61	104283.0
62-63	98067.0
64-65	102374.0
66-67	108916.0
68-69	111741.0
70-71	109750.0
72-73	104497.0
74-75	105133.0
76-77	103489.0
78-79	111282.0
80-81	113161.0
82-83	107699.0
84-85	105240.0
86-87	110395.0
88-89	118344.0
90-91	127006.0
92-93	131876.0
94-95	144062.0
96-97	218718.0
98-99	948321.0
100-101	6905663.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.31206619027097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06227493495241	76.83398760497042
2	6.914535867774117	11.797866832530964
3	1.6878494521593561	4.319817725454947
4	0.6009701245717181	2.050800121833512
5	0.28568110518464485	1.218602267741109
6	0.15322460887328518	0.7843144784505649
7	0.08465907950307298	0.5055708695921503
8	0.05347092766846527	0.364937225640584
9	0.0331960910085132	0.2548824402038883
>10	0.1205393039147734	1.6026653771413133
>50	0.003153476966807513	0.1805401420051375
>100	3.93930134430422E-4	0.051011064926601145
>500	3.406610246837502E-5	0.018730634049421716
>1k	1.7031185984628587E-5	0.01627321545941783
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.761739306888593E-6
2	0.0	2.628521792066578E-5	0.0	0.0	8.761739306888593E-6
3	0.0	3.504695722755437E-5	0.0	0.0	8.761739306888593E-6
4	0.0	8.761739306888594E-5	0.0	1.7523478613777186E-5	8.761739306888593E-6
5	0.0	8.761739306888594E-5	0.0	1.7523478613777186E-5	8.761739306888593E-6
6	6.133217514822015E-5	8.761739306888594E-5	0.0	3.504695722755437E-5	8.761739306888593E-6
7	6.133217514822015E-5	8.761739306888594E-5	0.0	4.380869653444297E-5	1.7523478613777186E-5
8	6.133217514822015E-5	9.637913237577453E-5	0.0	4.380869653444297E-5	1.7523478613777186E-5
9	6.133217514822015E-5	1.5771130752399468E-4	0.0	6.133217514822015E-5	3.504695722755437E-5
10-11	6.133217514822015E-5	1.5771130752399468E-4	0.0	8.323652341544165E-5	3.504695722755437E-5
12-13	6.133217514822015E-5	1.5771130752399468E-4	0.0	1.0952174133610742E-4	3.504695722755437E-5
14-15	6.571304480166445E-5	2.36566961285992E-4	0.0	1.358069592567732E-4	3.942782688099867E-5
16-17	1.1390261098955171E-4	2.497095702463249E-4	0.0	1.401878289102175E-4	4.380869653444297E-5
18-19	1.1390261098955171E-4	2.716139185135464E-4	0.0	1.5771130752399468E-4	5.257043584133156E-5
20-21	1.1390261098955171E-4	2.935182667807679E-4	0.0	2.0152000405843765E-4	6.133217514822015E-5
22-23	1.18283480642996E-4	3.2418435435487794E-4	0.0	2.2342435232565913E-4	6.571304480166445E-5
24-25	1.270452199498846E-4	3.7237392054276523E-4	0.0	2.36566961285992E-4	7.009391445510874E-5
26-27	1.314260896033289E-4	3.942782688099867E-4	0.0	2.672330488601021E-4	7.009391445510874E-5
28-29	1.314260896033289E-4	3.942782688099867E-4	0.0	6.921774052441989E-4	7.447478410855304E-5
30-31	1.314260896033289E-4	4.2056348673065246E-4	0.0	0.0018005374275656058	7.885565376199734E-5
32-33	1.314260896033289E-4	4.2056348673065246E-4	0.0	0.0038726887736447585	1.1390261098955171E-4
34-35	1.445686985636618E-4	4.906574011857612E-4	0.0	0.006619494046354332	1.18283480642996E-4
36-37	1.489495682171061E-4	5.651321852943143E-4	0.0	0.010597323691681754	1.226643502964403E-4
38-39	1.5771130752399468E-4	5.826556639080914E-4	0.0	0.01675244555477099	1.226643502964403E-4
40-41	1.7523478613777187E-4	6.133217514822016E-4	0.0	0.023301845686670215	1.226643502964403E-4
42-43	1.7523478613777187E-4	6.571304480166445E-4	0.0	0.029141544934711463	1.270452199498846E-4
44-45	1.7961565579121617E-4	7.66652189352752E-4	0.0	0.0356821833273038	1.314260896033289E-4
46-47	1.8399652544466046E-4	8.498887127681936E-4	0.0	0.0430902339112781	1.445686985636618E-4
48-49	1.8399652544466046E-4	8.498887127681936E-4	0.0	0.05072608971723151	1.5771130752399468E-4
50-51	2.0590087371188194E-4	0.0010076000202921883	0.0	0.05839261161075903	1.5771130752399468E-4
52-53	2.1028174336532623E-4	0.001020742629252521	0.0	0.06549838218864568	1.8399652544466046E-4
54-55	2.1028174336532623E-4	0.0010251234989059653	0.0	0.07241139450178077	2.1466261301877052E-4
56-57	2.1028174336532623E-4	0.0010514087168266312	0.0	0.07966611464788453	2.2342435232565913E-4
58-59	2.1028174336532623E-4	0.0010601704561335199	0.0	0.08657036522171274	2.2780522197910343E-4
60-61	2.2780522197910343E-4	0.0010733130650938528	0.0	0.09411860363459726	2.2780522197910343E-4
62-63	2.2780522197910343E-4	0.00109083654370763	0.0	0.1013908472593148	2.2780522197910343E-4
64-65	2.2780522197910343E-4	0.0011915965457368488	0.0	0.10792272391260024	2.36566961285992E-4
66-67	2.2780522197910343E-4	0.0012047391546971815	0.0	0.11432317447628237	2.36566961285992E-4
68-69	2.2780522197910343E-4	0.0012266435029644031	0.0	0.12088571721714192	2.36566961285992E-4
70-71	2.2780522197910343E-4	0.001239786111924736	0.0	0.1272029312574086	2.36566961285992E-4
72-73	2.36566961285992E-4	0.0012441669815781802	0.0	0.1320306496155042	2.453287005928806E-4
74-75	2.36566961285992E-4	0.0012835948084591788	0.0	0.13626695057038485	2.453287005928806E-4
76-77	2.36566961285992E-4	0.0013405461139539547	0.0	0.13975850368417997	2.5409043989976923E-4
78-79	2.36566961285992E-4	0.0013493078532608434	0.0	0.1431142498387183	2.5409043989976923E-4
80-81	2.36566961285992E-4	0.0013493078532608434	0.0	0.14473079074083922	2.584713095532135E-4
82-83	2.36566961285992E-4	0.0013536887229142877	0.0	0.14530030379578698	2.716139185135464E-4
84-85	2.36566961285992E-4	0.001358069592567732	0.0	0.1458172464148934	2.716139185135464E-4
86-87	2.36566961285992E-4	0.0013843548104883977	0.0	0.14604067076721908	2.716139185135464E-4
88-89	2.36566961285992E-4	0.001445686985636618	0.0	0.14606695598513975	2.716139185135464E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12075	0.0	47.954834	1
GTATCAA	21505	0.0	35.915096	1
TCAACGC	25390	0.0	29.520828	4
ATCAACG	25960	0.0	28.95393	3
CAACGCA	26010	0.0	28.849466	5
AACGCAG	27070	0.0	27.766558	6
TATCAAC	27465	0.0	27.56855	2
ACGCAGA	31825	0.0	23.591412	7
CGCAGAG	32465	0.0	23.086836	8
GTACATG	33600	0.0	23.011854	1
TACATGG	33560	0.0	22.586842	2
ACATGGG	34015	0.0	21.700394	3
GCAGAGT	38670	0.0	19.425713	9
CATGGGG	25620	0.0	19.273252	4
GAGTACT	22105	0.0	17.822018	12-13
AGAGTAC	34620	0.0	17.791359	10-11
CAGAGTA	38155	0.0	17.243553	10-11
GTGGTAT	6415	0.0	17.237331	1
ATGGGGG	16165	0.0	16.474037	5
TGGTATC	5910	0.0	16.42523	2
>>END_MODULE