FastQCFastQC Report
Fri 27 May 2016
SRR2140026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5891891
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62900.043.6252821
GTATCAA114350.035.4464451
ATCAACG128900.030.5358983
TCAACGC129200.030.4649964
CAACGCA131250.030.0526455
TATCAAC138200.028.9054932
AACGCAG136900.028.9040036
ACGCAGA159800.024.6834167
CGCAGAG163300.024.0771268
GTACATG176850.022.3281121
TACATGG173700.022.1309852
ACATGGG178500.020.996353
GCAGAGT194650.020.1993039
CATGGGG125700.019.2338664
AGAGTAC174300.017.74613210-11
GAGTACT108300.017.57312-13
CAGAGTA191350.017.26877610-11
TATACCG4251.8189894E-1216.7313255
GAGTACA124350.015.9783851
ATGGGGG81450.015.61179355
Produced by FastQC (version 0.11.5)