##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140026_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5891891
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42082448572114	33.0	31.0	34.0	31.0	34.0
2	32.61796696510509	34.0	31.0	34.0	31.0	34.0
3	32.71513882385129	34.0	31.0	34.0	31.0	34.0
4	36.20254617744965	37.0	37.0	37.0	35.0	37.0
5	36.000304146835035	37.0	35.0	37.0	35.0	37.0
6	36.06469722538995	37.0	35.0	37.0	35.0	37.0
7	36.053324306237165	37.0	35.0	37.0	35.0	37.0
8	36.05796644914171	37.0	35.0	37.0	35.0	37.0
9	37.94308499597158	39.0	38.0	39.0	35.0	39.0
10-11	37.79087138577411	39.0	38.0	39.0	35.0	39.0
12-13	37.79747138906677	39.0	38.0	39.0	35.0	39.0
14-15	39.15684506043985	40.0	39.0	41.0	36.0	41.0
16-17	39.121098727047055	40.0	39.0	41.0	36.0	41.0
18-19	39.160620418809515	40.0	39.0	41.0	36.0	41.0
20-21	39.169077172170915	40.0	39.0	41.0	36.0	41.0
22-23	39.10738723472383	40.0	39.0	41.0	36.0	41.0
24-25	39.13403490955576	40.0	39.0	41.0	36.0	41.0
26-27	39.02958007571449	40.0	39.0	41.0	36.0	41.0
28-29	38.951173563288734	40.0	39.0	41.0	35.0	41.0
30-31	38.85591918150182	40.0	38.0	41.0	35.0	41.0
32-33	38.78081191450674	40.0	38.0	41.0	35.0	41.0
34-35	38.73340621801406	40.0	38.0	41.0	35.0	41.0
36-37	38.72073221548395	40.0	38.0	41.0	35.0	41.0
38-39	38.64406999268419	40.0	38.0	41.0	35.0	41.0
40-41	38.61890976046266	40.0	38.0	41.0	35.0	41.0
42-43	38.57139935484989	40.0	38.0	41.0	35.0	41.0
44-45	38.479144172794015	40.0	38.0	41.0	34.0	41.0
46-47	38.41551001689442	40.0	38.0	41.0	34.0	41.0
48-49	38.42291478433346	40.0	38.0	41.0	34.0	41.0
50-51	38.360311545331214	40.0	38.0	41.0	34.0	41.0
52-53	38.18489123917857	40.0	37.5	41.0	34.0	41.0
54-55	38.02467091222367	40.0	37.0	41.0	33.0	41.0
56-57	37.90633258773923	40.0	37.0	41.0	33.0	41.0
58-59	37.752809474707796	39.0	36.5	41.0	33.0	41.0
60-61	37.58112797315667	39.0	36.0	41.0	33.0	41.0
62-63	37.42172351040959	39.0	36.0	41.0	33.0	41.0
64-65	37.21716061112442	39.0	35.0	40.0	32.0	41.0
66-67	36.934347332030356	38.0	35.0	40.0	32.0	41.0
68-69	36.68852864965232	37.5	35.0	40.0	32.0	41.0
70-71	36.39736075408077	37.0	35.0	39.5	32.0	41.0
72-73	36.095179389036964	36.5	35.0	39.0	31.0	41.0
74-75	35.66028531165399	36.0	34.5	39.0	31.0	40.5
76-77	33.73659672995183	34.5	32.0	36.5	28.0	39.0
78-79	34.794101437829745	35.0	34.0	37.0	30.0	39.0
80-81	34.763977096218234	35.0	34.0	37.0	31.0	39.0
82-83	34.51946495534842	35.0	34.0	36.5	31.0	39.0
84-85	34.25778696699269	35.0	34.0	36.0	30.5	37.0
86-87	34.019932401801526	35.0	34.0	36.0	30.0	37.0
88-89	33.86227854899521	35.0	34.0	35.0	30.0	37.0
90-91	33.78036798936362	35.0	34.0	35.0	30.0	36.0
92-93	33.659648060994485	35.0	34.0	35.0	30.0	36.0
94-95	33.561443748358386	35.0	34.0	35.0	30.0	36.0
96-97	33.54698833935454	35.0	34.0	35.0	30.0	36.0
98-99	33.66184953207591	35.0	34.0	35.0	31.0	35.0
100-101	32.44656128044207	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	9.0
20	29.0
21	96.0
22	479.0
23	1501.0
24	3903.0
25	8677.0
26	15878.0
27	26259.0
28	39008.0
29	54666.0
30	73534.0
31	96778.0
32	128751.0
33	175777.0
34	258113.0
35	401445.0
36	724164.0
37	1382376.0
38	1739085.0
39	749737.0
40	11624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00527470722049	20.295385641044618	13.42456946335226	24.274770188382643
2	14.716555520288951	21.597384764487927	41.01601763858219	22.67004207664093
3	18.158822014867553	26.593940722935983	30.451004609555742	24.79623265264072
4	12.250584110365036	18.007707575155308	39.53737237065473	30.20433594382493
5	12.808044140667233	38.30887231281095	36.04900362209688	12.834079924424943
6	29.552990712149967	38.510895737887886	19.11842904086311	12.817684509099031
7	26.206041937949863	32.75027530011447	23.828311171278084	17.215371590657583
8	24.3917544705603	36.810321695000006	21.652924739931937	17.144999094507764
9	25.7938158712766	16.347914904710525	21.372143976235805	36.486125247777075
10-11	23.988251971101935	26.83569528378199	29.51902295395597	19.657029791160106
12-13	24.79342770495821	24.465620843859114	29.44225480338717	21.29869664779551
14-15	21.886900628397733	25.80901254173216	26.98316049545736	25.320926334412757
16-17	21.533249008060686	28.866543587410952	27.665337009021336	21.934870395507026
18-19	21.545663353242617	28.30652332162968	29.133167942176797	21.014645382950906
20-21	22.384196896358436	27.58111404700993	28.917417131344436	21.1172719252872
22-23	22.070639715316553	27.49594319978944	28.739018588331135	21.69439849656287
24-25	21.7982123824162	27.550856943477005	28.905887541476133	21.745043132630666
26-27	21.76343342653019	27.77328024622994	28.738767967006275	21.724518360233592
28-29	21.694679591154227	27.91497238510508	28.75844095358819	21.631907070152497
30-31	22.034531214768744	27.726016488046024	28.660154104404654	21.57929819278057
32-33	21.766984258668913	27.89226794488699	28.62454753168452	21.71620026475958
34-35	21.860836372621186	27.920904433827452	28.653536600562425	21.564722592988932
36-37	21.848239194285107	27.874876357826047	28.61486164298716	21.66202280490169
38-39	21.69954362004548	27.980837799553537	28.71901277919368	21.6006058012073
40-41	21.973586121837002	27.842843300587617	28.558877847354967	21.624692730220413
42-43	21.858916227624317	27.901972112296892	28.399835156835152	21.83927650324364
44-45	21.947144115416958	28.06868769314918	28.21412656914154	21.77004162229233
46-47	21.941341677951456	28.018416847980017	28.027983994937884	22.012257479130646
48-49	21.934019791565966	28.035889308860558	28.08272268472965	21.94736821484383
50-51	21.872261010943244	28.177254937502695	27.971638487891987	21.978845563662073
52-53	22.057231010603243	28.111988067864026	27.827425752559265	22.00335516897347
54-55	22.056623111342834	27.99321230866272	27.93343423839017	22.016730341604276
56-57	22.051579293684963	28.10788107293945	27.83958825793314	22.000951375442447
58-59	21.996859518711922	28.1796220007414	27.848595440786784	21.97492303975989
60-61	22.071272058969296	28.124192206073793	27.851451823121653	21.953083911835257
62-63	22.042746690893964	28.138471697577465	27.808369205459865	22.010412406068706
64-65	21.991045368734746	28.114817031695555	27.84582851294189	22.04830908662781
66-67	22.122908215474965	28.16146769895641	27.62952165438786	22.08610243118077
68-69	22.069945273632346	28.15158025474851	27.69979567209565	22.078678799523487
70-71	22.024300150057734	27.910748949405416	27.802156026073423	22.26279487446343
72-73	22.059218326099316	28.017182802440498	27.795701860723383	22.127897010736802
74-75	21.97089432700211	28.133792815543586	27.712481253507633	22.18283160394667
76-77	22.027870863816133	28.06815236112078	27.678037991630582	22.225938783432504
78-79	22.053971498318965	28.096892096455424	27.63376815267623	22.215368252549386
80-81	21.994660952057206	28.10032092111281	27.65210022411035	22.252917902719638
82-83	22.06126078615856	28.062634240883273	27.794224609565312	22.08188036339286
84-85	22.216476009424188	28.02037074275227	27.663473603245976	22.09967964457757
86-87	21.983902639263462	28.10819858611496	27.718990205740372	22.188908568881207
88-89	22.062203442662696	28.05666193329408	27.705053163855503	22.176081460187717
90-91	22.12979603747702	28.03864429702456	27.758369424900337	22.073190240598077
92-93	22.119728532357964	28.022162451215117	27.72874763837511	22.129361378051804
94-95	21.99309032120646	28.126383839910314	27.763172218494645	22.117353620388577
96-97	21.995316565181547	28.132707525569632	27.837511379576245	22.034464529672576
98-99	22.21751230400105	28.731234976963442	27.839402896012928	21.211849823022575
100-101	26.19122919296987	35.77385591943561	28.52991738198939	9.504997505605125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1329.0
1	1158.0
2	1516.5
3	2181.0
4	2954.0
5	4657.0
6	6084.5
7	7134.5
8	7822.0
9	7568.0
10	6811.5
11	6205.5
12	6794.5
13	8934.0
14	13563.5
15	19614.0
16	24453.0
17	27338.5
18	28023.0
19	27077.0
20	25764.0
21	24971.0
22	25637.0
23	28953.5
24	35367.0
25	44678.0
26	56437.0
27	69712.5
28	83814.5
29	98560.0
30	112203.5
31	125225.0
32	139187.5
33	152949.0
34	166144.0
35	178952.5
36	193038.0
37	205181.5
38	213059.5
39	219470.5
40	226560.0
41	232786.5
42	236901.0
43	241394.5
44	245640.5
45	249244.5
46	251046.0
47	251572.0
48	250566.0
49	246406.5
50	241139.0
51	232879.5
52	221541.5
53	210056.0
54	197131.5
55	181909.0
56	167129.0
57	151579.0
58	134331.0
59	116495.5
60	97481.0
61	79454.5
62	63469.5
63	49651.5
64	37606.5
65	28121.0
66	21365.5
67	16440.0
68	12541.5
69	9038.0
70	6465.5
71	4651.0
72	3251.0
73	2222.0
74	1534.0
75	1041.0
76	715.5
77	502.5
78	348.0
79	227.5
80	154.0
81	106.5
82	80.5
83	58.0
84	34.5
85	20.0
86	11.0
87	9.5
88	9.0
89	7.5
90	6.5
91	5.5
92	2.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004429817184330124
3	0.0
4	4.2431199083621876E-4
5	0.0
6	0.0
7	0.0023591746690493766
8	0.0011541286150745151
9	3.39449592668975E-5
10-11	1.5275231670103877E-4
12-13	1.1880735743414127E-4
14-15	2.545871945017313E-5
16-17	3.9885327138604565E-4
18-19	0.0
20-21	5.9406300224844354E-5
22-23	0.0
24-25	0.0
26-27	6.709449172058219E-4
28-29	1.359814473712279E-4
30-31	4.2559443199911745E-5
32-33	0.001852087827882491
34-35	5.566822423503295E-4
36-37	0.0014459857198581695
38-39	0.003771807877806604
40-41	1.1366586310730469E-4
42-43	9.34796283638005E-4
44-45	0.0019861733831390803
46-47	1.0810323859285617E-4
48-49	2.824573056670958E-4
50-51	2.9473691966761044E-4
52-53	0.0
54-55	1.785538920284615E-4
56-57	0.0017005523089890244
58-59	0.003372511799707773
60-61	0.0010203608637383865
62-63	0.0010610956264291017
64-65	0.0
66-67	1.1049759275971555E-4
68-69	0.0
70-71	6.176482487047143E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.109772602044978E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	165.0
20-21	501.0
22-23	935.0
24-25	2339.0
26-27	3433.0
28-29	6837.0
30-31	15205.0
32-33	18639.0
34-35	25491.0
36-37	39790.0
38-39	48284.0
40-41	48034.0
42-43	53259.0
44-45	62414.0
46-47	63888.0
48-49	59923.0
50-51	55288.0
52-53	53063.0
54-55	56788.0
56-57	59164.0
58-59	58520.0
60-61	57160.0
62-63	54421.0
64-65	56104.0
66-67	59422.0
68-69	60489.0
70-71	60330.0
72-73	57222.0
74-75	57754.0
76-77	56240.0
78-79	59964.0
80-81	60691.0
82-83	57706.0
84-85	56667.0
86-87	59214.0
88-89	63718.0
90-91	66870.0
92-93	69184.0
94-95	74299.0
96-97	111724.0
98-99	477982.0
100-101	3482770.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.48370327709478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.477728477066	80.94288183027349
2	6.324658347902832	11.192583851696694
3	1.3493521111080782	3.5818701544682585
4	0.44288078482386795	1.5675092780592796
5	0.17110294687677446	0.756991119064051
6	0.0900790091796702	0.47823145918491794
7	0.05092351214535868	0.3154130656948214
8	0.024515797114366637	0.17353988139752588
9	0.01642911230315329	0.13083378283244546
>10	0.050885223763036856	0.673637134146311
>50	9.022911382678352E-4	0.054109915858522865
>100	4.898728587171619E-4	0.09043965351592446
>500	2.625686038634219E-5	0.01691365489204814
>1k	2.6256859517950687E-5	0.025045218915675377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.697247963344875E-5	0.0	0.0	0.0
3	0.0	1.697247963344875E-5	0.0	0.0	0.0
4	0.0	1.0183487780069251E-4	0.0	0.0	0.0
5	0.0	1.0183487780069251E-4	0.0	0.0	0.0
6	0.0	1.0183487780069251E-4	0.0	0.0	0.0
7	0.0	1.1880735743414126E-4	0.0	0.0	0.0
8	0.0	1.1880735743414126E-4	0.0	0.0	0.0
9	0.0	2.7155967413518E-4	0.0	0.0	0.0
10-11	1.697247963344875E-5	2.885321537686288E-4	0.0	5.940367871707064E-5	0.0
12-13	1.697247963344875E-5	2.9701839358535313E-4	0.0	8.486239816724377E-5	0.0
14-15	1.697247963344875E-5	3.818807917525969E-4	0.0	8.486239816724377E-5	8.486239816724376E-6
16-17	1.697247963344875E-5	3.903670315693213E-4	0.0	1.0183487780069251E-4	3.39449592668975E-5
18-19	1.697247963344875E-5	4.158257510194944E-4	0.0	1.1880735743414126E-4	3.39449592668975E-5
20-21	1.697247963344875E-5	4.243119908362188E-4	0.0	1.2729359725086563E-4	4.2431199083621876E-5
22-23	1.697247963344875E-5	4.582569501031163E-4	0.0	1.5275231670103877E-4	5.940367871707063E-5
24-25	1.697247963344875E-5	5.091743890034625E-4	0.0	1.782110361512119E-4	7.637615835051939E-5
26-27	1.697247963344875E-5	5.600918279038089E-4	0.0	2.9701839358535313E-4	8.486239816724377E-5
28-29	1.697247963344875E-5	5.770643075372576E-4	0.0	8.146790224055401E-4	8.486239816724377E-5
30-31	1.697247963344875E-5	6.873854251546745E-4	0.0	0.0022403673116152354	8.486239816724377E-5
32-33	1.697247963344875E-5	7.128441446048475E-4	0.0	0.004684404378831856	8.486239816724377E-5
34-35	1.697247963344875E-5	8.061927825888156E-4	0.0	0.007764909432302804	1.0183487780069251E-4
36-37	1.697247963344875E-5	9.25000140022957E-4	0.0	0.012712387245453115	1.0183487780069251E-4
38-39	1.697247963344875E-5	9.674313391065789E-4	0.0	0.02090160866859214	1.0183487780069251E-4
40-41	1.697247963344875E-5	0.001060779977090547	0.0	0.029175692489898403	1.0183487780069251E-4
42-43	1.697247963344875E-5	0.0010947249363574445	0.0	0.03760252862790571	1.0183487780069251E-4
44-45	1.697247963344875E-5	0.0011456423752577907	0.0	0.04656399787436665	1.0183487780069251E-4
46-47	1.697247963344875E-5	0.0011711010947079638	0.0	0.05578005431532932	1.0183487780069251E-4
48-49	1.697247963344875E-5	0.001179587334524688	0.0	0.06547134018602856	1.0183487780069251E-4
50-51	1.697247963344875E-5	0.0013153671715922782	0.0	0.07458556174919054	1.0183487780069251E-4
52-53	1.697247963344875E-5	0.0013238534114090026	0.0	0.0833094162807832	1.1880735743414126E-4
54-55	1.697247963344875E-5	0.0013238534114090026	0.0	0.09201629833274241	1.3577983706759E-4
56-57	1.697247963344875E-5	0.0013408258910424513	0.0	0.10022249223551488	1.3577983706759E-4
58-59	1.697247963344875E-5	0.0013577983706759003	0.0	0.10774130071313268	1.3577983706759E-4
60-61	1.697247963344875E-5	0.0013577983706759003	0.0	0.11503098071569892	1.3577983706759E-4
62-63	1.697247963344875E-5	0.0013832570901260734	0.0	0.12203212856449652	1.3577983706759E-4
64-65	1.697247963344875E-5	0.0014766057281100414	0.0	0.1291181388114614	1.3577983706759E-4
66-67	1.697247963344875E-5	0.0014935782077434901	0.0	0.1357374058685064	1.3577983706759E-4
68-69	1.697247963344875E-5	0.0015105506873769388	0.0	0.1431119482692399	1.3577983706759E-4
70-71	1.697247963344875E-5	0.0015105506873769388	0.0	0.14947662813178314	1.3577983706759E-4
72-73	1.697247963344875E-5	0.0015190369271936633	0.0	0.15504360145155435	1.6972479633448753E-4
74-75	1.697247963344875E-5	0.001552981886460561	0.0	0.15926974888028309	1.6972479633448753E-4
76-77	1.697247963344875E-5	0.0016378442846278045	0.0	0.16305461183854214	1.6972479633448753E-4
78-79	1.697247963344875E-5	0.0016802754837114263	0.0	0.1667206674393671	1.6972479633448753E-4
80-81	1.697247963344875E-5	0.0016802754837114263	0.0	0.16841791540271195	1.782110361512119E-4
82-83	1.697247963344875E-5	0.0016887617235281508	0.0	0.16905438338896628	2.0366975560138503E-4
84-85	1.697247963344875E-5	0.0017057342031615995	0.0	0.16949566785943596	2.0366975560138503E-4
86-87	1.697247963344875E-5	0.0017566516420619458	0.0	0.16966539265577044	2.0366975560138503E-4
88-89	1.697247963344875E-5	0.0018415140402291896	0.0	0.16972479633448753	2.0366975560138503E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6290	0.0	43.625282	1
GTATCAA	11435	0.0	35.446445	1
ATCAACG	12890	0.0	30.535898	3
TCAACGC	12920	0.0	30.464996	4
CAACGCA	13125	0.0	30.052645	5
TATCAAC	13820	0.0	28.905493	2
AACGCAG	13690	0.0	28.904003	6
ACGCAGA	15980	0.0	24.683416	7
CGCAGAG	16330	0.0	24.077126	8
GTACATG	17685	0.0	22.328112	1
TACATGG	17370	0.0	22.130985	2
ACATGGG	17850	0.0	20.99635	3
GCAGAGT	19465	0.0	20.199303	9
CATGGGG	12570	0.0	19.233866	4
AGAGTAC	17430	0.0	17.746132	10-11
GAGTACT	10830	0.0	17.573	12-13
CAGAGTA	19135	0.0	17.268776	10-11
TATACCG	425	1.8189894E-12	16.731325	5
GAGTACA	12435	0.0	15.978385	1
ATGGGGG	8145	0.0	15.6117935	5
>>END_MODULE