FastQCFastQC Report
Fri 27 May 2016
SRR2140025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5799669
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59700.044.16621
GTATCAA105050.035.4652251
TCAACGC122450.029.300044
ATCAACG122900.029.1927593
CAACGCA125800.028.5529565
AACGCAG130300.027.7269446
TATCAAC130800.027.7183132
ACGCAGA152400.023.67887
CGCAGAG155100.023.2659958
GTACATG162750.022.6097031
TACATGG160900.022.3773962
ACATGGG164300.021.3289893
CATGGGG123150.019.6820494
GCAGAGT189900.018.9801049
GAGTACT105250.017.79647812-13
AGAGTAC164150.017.7133910-11
CAGAGTA180900.016.89191610-11
TATACCG3901.10958354E-1016.0455725
GTACTTT117500.015.90635214-15
GAGTACA118150.015.5722781
Produced by FastQC (version 0.11.5)