##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140025_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5799669
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.429834530211984	33.0	31.0	34.0	31.0	34.0
2	32.61613912793989	34.0	31.0	34.0	31.0	34.0
3	32.70905494779099	34.0	31.0	34.0	31.0	34.0
4	36.195021302077755	37.0	37.0	37.0	35.0	37.0
5	35.99950410963108	37.0	35.0	37.0	35.0	37.0
6	36.06579892749052	37.0	35.0	37.0	35.0	37.0
7	36.04999078395681	37.0	35.0	37.0	35.0	37.0
8	36.053173551800974	37.0	35.0	37.0	35.0	37.0
9	37.929213718920856	39.0	38.0	39.0	35.0	39.0
10-11	37.77589117585848	39.0	37.5	39.0	35.0	39.0
12-13	37.779111273419225	39.0	38.0	39.0	35.0	39.0
14-15	39.13744991308987	40.0	39.0	41.0	36.0	41.0
16-17	39.099461452024244	40.0	38.5	41.0	36.0	41.0
18-19	39.12917633747719	40.0	39.0	41.0	36.0	41.0
20-21	39.13769281766898	40.0	39.0	41.0	36.0	41.0
22-23	39.07255214995281	40.0	39.0	41.0	36.0	41.0
24-25	39.09362866304657	40.0	39.0	41.0	36.0	41.0
26-27	38.98588483483998	40.0	39.0	41.0	35.5	41.0
28-29	38.905993790391534	40.0	38.0	41.0	35.0	41.0
30-31	38.80552264833864	40.0	38.0	41.0	35.0	41.0
32-33	38.732933420396165	40.0	38.0	41.0	35.0	41.0
34-35	38.683273298167975	40.0	38.0	41.0	35.0	41.0
36-37	38.66539553719733	40.0	38.0	41.0	35.0	41.0
38-39	38.585749186270654	40.0	38.0	41.0	35.0	41.0
40-41	38.55369042663634	40.0	38.0	41.0	35.0	41.0
42-43	38.50174788204401	40.0	38.0	41.0	34.0	41.0
44-45	38.40630590513713	40.0	38.0	41.0	34.0	41.0
46-47	38.33228869525918	40.0	38.0	41.0	34.0	41.0
48-49	38.32855154478666	40.0	38.0	41.0	34.0	41.0
50-51	38.253045496753266	40.0	38.0	41.0	34.0	41.0
52-53	38.06435080712768	40.0	37.0	41.0	33.0	41.0
54-55	37.89001517650033	40.0	37.0	41.0	33.0	41.0
56-57	37.758172903600745	39.0	36.5	41.0	33.0	41.0
58-59	37.589199937305715	39.0	36.0	41.0	33.0	41.0
60-61	37.39974652583989	39.0	36.0	41.0	32.5	41.0
62-63	37.225780371240496	39.0	35.0	40.0	32.0	41.0
64-65	37.0025830399889	38.5	35.0	40.0	32.0	41.0
66-67	36.71154235750603	38.0	35.0	40.0	32.0	41.0
68-69	36.46005843191093	37.0	35.0	40.0	31.5	41.0
70-71	36.1683196919614	37.0	35.0	39.0	31.0	41.0
72-73	35.861872606601466	36.0	35.0	39.0	31.0	41.0
74-75	35.42984453451595	36.0	34.0	39.0	31.0	40.0
76-77	33.52040150761897	34.5	32.0	36.5	28.0	39.0
78-79	34.60919238870957	35.0	34.0	37.0	30.0	39.0
80-81	34.59641373000372	35.0	34.0	37.0	30.5	39.0
82-83	34.36593042456796	35.0	34.0	36.0	30.5	38.5
84-85	34.11243921089543	35.0	34.0	36.0	30.0	37.0
86-87	33.89498925424245	35.0	34.0	35.5	30.0	37.0
88-89	33.75074104638587	35.0	34.0	35.0	30.0	36.5
90-91	33.675909332739806	35.0	34.0	35.0	30.0	36.0
92-93	33.560890108623454	35.0	34.0	35.0	30.0	36.0
94-95	33.46691990114547	35.0	33.0	35.0	30.0	36.0
96-97	33.46034193215548	35.0	33.0	35.0	30.0	35.5
98-99	33.58667085629138	35.0	33.0	35.0	30.0	35.0
100-101	32.35024451728479	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	9.0
20	29.0
21	132.0
22	469.0
23	1624.0
24	4277.0
25	9210.0
26	16741.0
27	27545.0
28	40916.0
29	56693.0
30	75883.0
31	100034.0
32	132047.0
33	180745.0
34	267198.0
35	416525.0
36	754498.0
37	1391076.0
38	1621777.0
39	689846.0
40	12394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47875180462885	19.884565826084213	13.129852755390006	24.50682961389693
2	15.0898619287488	21.404777082858786	40.38785411655729	23.117506871835122
3	18.451628343489375	26.17191642209803	30.131661007433703	25.244794226978897
4	12.560564062207957	17.64196216181545	38.846142970154716	30.951330805821875
5	13.159233742477372	37.95601093786559	35.712831197780424	13.17192412187661
6	30.259864830217037	37.966821899663586	18.694911726858894	13.078401543260487
7	26.701010787585282	32.31351054403136	23.44709586299734	17.538382805386014
8	24.793038021827652	36.44044709166952	21.350379059579115	17.416135826923714
9	25.92586845132209	16.100245824628086	20.980046599259303	36.993839124790526
10-11	24.394156243138067	26.507556176358282	29.110053788502583	19.988233792001065
12-13	25.06738323716106	24.10651689701917	29.120093550279307	21.706006315540456
14-15	22.163020552471327	25.441251588991953	26.533150391811255	25.86257746672546
16-17	21.973638620273935	28.51363575976068	27.166642656632284	22.3460829633331
18-19	21.96930548967536	27.92702480089812	28.639755130853157	21.463914578573362
20-21	22.780914823846686	27.200044487188162	28.388904240034247	21.630136448930905
22-23	22.491232466117825	27.143127764953785	28.248044404294433	22.11759536463396
24-25	22.20544166221207	27.219936995709958	28.412354332980932	22.162267009097036
26-27	22.214057850917353	27.372418084684508	28.254808686231474	22.15871537816666
28-29	22.136997367902747	27.517759511293207	28.256599735831827	22.08864338497222
30-31	22.45069042656809	27.35223387342701	28.143883682526415	22.05319201747848
32-33	22.19724515287935	27.53084050406291	28.1087771846049	22.16313715845284
34-35	22.26008645332509	27.54703310122238	28.19931431889289	21.993566126559646
36-37	22.27340432510441	27.486226575995452	28.117892987558218	22.122476111341918
38-39	22.137971153862253	27.594266023534153	28.183100119509984	22.084662703093606
40-41	22.36151045922253	27.41195051778444	28.120672576771856	22.105866446221174
42-43	22.22118540071657	27.567670530993045	27.964330949839056	22.24681311845133
44-45	22.34662260387284	27.681930579010388	27.78184861308844	22.189598204028336
46-47	22.40090347622991	27.576031656095957	27.570052553836643	22.45301231383749
48-49	22.36038655225765	27.6944691370418	27.580062589290407	22.365081721410146
50-51	22.30542962218178	27.82227713147182	27.497695568255036	22.374597678091362
52-53	22.480795114638354	27.7123623722767	27.360048638035718	22.446793875049227
54-55	22.441561244598777	27.62490324472197	27.444634015550562	22.48890149512869
56-57	22.408261464360294	27.73765515829839	27.409753069375565	22.44433030796575
58-59	22.43892274000394	27.790049546132273	27.37909367850567	22.391934035358123
60-61	22.49279848185093	27.738648725240168	27.382926374175504	22.385626418733402
62-63	22.467953030239922	27.75150667228813	27.36576540510424	22.41477489236771
64-65	22.41800962279974	27.787161940447845	27.337432254815386	22.45739618193703
66-67	22.494742687072865	27.746908344592136	27.19522112782601	22.563127840508994
68-69	22.442579056861305	27.770947305213152	27.300492476382658	22.48598116154288
70-71	22.414642697531644	27.512321993118043	27.372124349196405	22.70091096015391
72-73	22.471760310040974	27.6266218268237	27.324629045399707	22.576988817735618
74-75	22.375012413659377	27.741756418585805	27.29387802290033	22.58935314485448
76-77	22.479674898232027	27.69357732102779	27.23717226414486	22.58957551659532
78-79	22.455145523948303	27.692172438557073	27.21193709026831	22.64074494722631
80-81	22.407900618030567	27.724921056399992	27.218944860520537	22.648233465048904
82-83	22.470445816050315	27.672099568233076	27.356998651066956	22.50045596464965
84-85	22.579116105704756	27.655829591542048	27.224695662433678	22.540358640319518
86-87	22.418249136058293	27.66869811933776	27.336731378559655	22.576321366044297
88-89	22.441417878148624	27.698601823151293	27.265417523915165	22.594562774784922
90-91	22.520007697607088	27.684582203313646	27.306819989264362	22.4885901098149
92-93	22.52889133369036	27.65033307727538	27.29835436320326	22.522421225830996
94-95	22.38138569867712	27.697989963248858	27.380844697037848	22.53977964103617
96-97	22.377257156744975	27.702245127414145	27.411153542768307	22.50934417307257
98-99	22.651823155082425	28.319885866654943	27.421067453518823	21.60722352474381
100-101	26.804093944194456	35.40331981174746	27.933225344081986	9.859360899976103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1209.0
1	1069.5
2	1440.0
3	2057.0
4	2775.0
5	4436.0
6	5833.5
7	6816.5
8	7398.0
9	7210.0
10	6494.0
11	5717.5
12	6100.5
13	8022.5
14	12382.5
15	18084.5
16	22590.5
17	25296.0
18	26058.5
19	25339.5
20	24173.0
21	23337.5
22	23783.5
23	26604.5
24	32253.5
25	40381.5
26	50579.5
27	61779.0
28	74329.0
29	88530.5
30	101726.5
31	113852.0
32	126456.5
33	139148.5
34	150936.5
35	162409.5
36	174755.0
37	185313.5
38	193498.5
39	200473.5
40	207720.5
41	215309.5
42	220900.0
43	227374.0
44	235251.5
45	242315.5
46	246399.0
47	248259.5
48	249764.0
49	248488.5
50	245338.5
51	240952.0
52	233354.0
53	223916.5
54	212878.0
55	198585.0
56	183842.0
57	167512.0
58	149992.0
59	131934.5
60	111479.0
61	91636.5
62	73697.0
63	57900.0
64	44088.5
65	33220.5
66	25352.0
67	19526.5
68	14808.0
69	10575.5
70	7538.5
71	5467.0
72	3889.5
73	2758.0
74	1904.0
75	1297.5
76	925.5
77	639.0
78	416.5
79	268.5
80	182.5
81	127.0
82	87.5
83	56.0
84	39.0
85	29.0
86	22.0
87	19.0
88	14.5
89	9.5
90	4.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0046899228214575695
3	3.448472662836448E-5
4	4.3105908285455603E-4
5	0.0
6	0.0
7	0.0022759919574720554
8	9.138452556516586E-4
9	1.0345417988509344E-4
10-11	1.638024514847313E-4
12-13	1.2069654319927568E-4
14-15	2.586354497127336E-5
16-17	1.8104481479891353E-4
18-19	0.0
20-21	1.6380914544734726E-4
22-23	0.0
24-25	0.0
26-27	6.643813673296417E-4
28-29	2.2450013103036495E-4
30-31	3.459439712603587E-5
32-33	0.001838485095219219
34-35	5.656541254765309E-4
36-37	0.0013906613504336284
38-39	0.003718850987844028
40-41	1.59966101405711E-4
42-43	8.694882846313822E-4
44-45	0.0018286738963862507
46-47	1.0987618419057511E-4
48-49	2.5003796872858475E-4
50-51	1.8721863963753723E-4
52-53	0.0
54-55	2.196536958930871E-4
56-57	0.0016412213003492904
58-59	0.0030758492712776022
60-61	0.0011457895739667918
62-63	0.0010088162550063754
64-65	2.019305367030963E-5
66-67	9.191544432742814E-5
68-69	2.0677511056265163E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	4.5144994435879434E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	149.0
20-21	515.0
22-23	991.0
24-25	2474.0
26-27	3434.0
28-29	7276.0
30-31	15032.0
32-33	18664.0
34-35	25271.0
36-37	40027.0
38-39	48025.0
40-41	47473.0
42-43	52292.0
44-45	61384.0
46-47	62514.0
48-49	59125.0
50-51	54316.0
52-53	51530.0
54-55	55716.0
56-57	58202.0
58-59	58597.0
60-61	57207.0
62-63	53800.0
64-65	55198.0
66-67	59381.0
68-69	60005.0
70-71	59212.0
72-73	55966.0
74-75	56001.0
76-77	55317.0
78-79	59166.0
80-81	60802.0
82-83	57207.0
84-85	56155.0
86-87	58836.0
88-89	62565.0
90-91	66393.0
92-93	68616.0
94-95	73902.0
96-97	110789.0
98-99	473085.0
100-101	3417059.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.30283873041648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.3769161073388	80.68841086709133
2	6.366930904710265	11.244361457726704
3	1.3706826772085943	3.6310551418837793
4	0.45428238874755233	1.604576980465739
5	0.18378319492660164	0.8114288911482199
6	0.09024446450627231	0.47813054373660946
7	0.04700027500348366	0.29051803927424896
8	0.03090475737425339	0.21831822451370775
9	0.022016808560324418	0.17497320260947022
>10	0.056033466979097994	0.7402873009170619
>50	9.279918169542823E-4	0.0537738571208949
>100	2.4984441740413804E-4	0.04963007348599465
>500	2.7118410440541086E-5	0.014535420026200995
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.896945325672897E-5	0.0	0.0	0.0
3	0.0	6.896945325672897E-5	0.0	0.0	0.0
4	0.0	1.7242363314182242E-4	0.0	1.724236331418224E-5	0.0
5	1.724236331418224E-5	1.7242363314182242E-4	0.0	1.724236331418224E-5	0.0
6	5.172708994254672E-5	1.8966599645600464E-4	0.0	1.724236331418224E-5	0.0
7	5.172708994254672E-5	1.8966599645600464E-4	0.0	1.724236331418224E-5	0.0
8	5.172708994254672E-5	2.0690835977018687E-4	0.0	1.724236331418224E-5	0.0
9	5.172708994254672E-5	3.4484726628364483E-4	0.0	1.724236331418224E-5	0.0
10-11	5.172708994254672E-5	3.4484726628364483E-4	0.0	5.172708994254672E-5	0.0
12-13	5.172708994254672E-5	3.6208962959782706E-4	0.0	5.172708994254672E-5	0.0
14-15	5.172708994254672E-5	4.3968026451164714E-4	0.0	6.896945325672897E-5	0.0
16-17	6.896945325672897E-5	4.5692262782582937E-4	0.0	8.621181657091121E-5	0.0
18-19	6.896945325672897E-5	5.000285361112849E-4	0.0	8.621181657091121E-5	0.0
20-21	6.896945325672897E-5	5.345132627396495E-4	0.0	9.483299822800232E-5	0.0
22-23	1.1207536154218456E-4	5.77619171025105E-4	0.0	1.2069654319927568E-4	0.0
24-25	1.3793890651345793E-4	6.207250793105606E-4	0.0	1.2069654319927568E-4	0.0
26-27	1.3793890651345793E-4	6.207250793105606E-4	0.0	1.4656008817054905E-4	0.0
28-29	1.3793890651345793E-4	6.293462609676518E-4	0.0	3.5346844794073595E-4	0.0
30-31	1.5518126982764016E-4	6.724521692531073E-4	0.0	0.001362146701820397	0.0
32-33	1.5518126982764016E-4	6.896945325672897E-4	0.0	0.003353639664608446	0.0
34-35	1.6380245148473128E-4	8.276334390807476E-4	0.0	0.006215871974762698	0.0
36-37	1.7242363314182242E-4	9.655723455942054E-4	0.0	0.010233342626967158	0.0
38-39	1.7242363314182242E-4	9.655723455942054E-4	0.0	0.016725092414756774	0.0
40-41	1.7242363314182242E-4	0.00115523834205021	0.0	0.02324270574751766	0.0
42-43	1.7242363314182242E-4	0.0011983442503356657	0.0	0.029691349627021815	0.0
44-45	1.8104481479891353E-4	0.0013535255201633058	0.0	0.03680382449412199	0.0
46-47	2.069083597701869E-4	0.0014656008817054904	0.0	0.04427838899082	0.0
48-49	2.3277190474146024E-4	0.0014656008817054904	0.0	0.052261603205286375	0.0
50-51	2.586354497127336E-4	0.0016294033331902217	0.0	0.060158605603181835	0.0
52-53	2.586354497127336E-4	0.0016380245148473129	0.0	0.06770213955313656	0.0
54-55	2.586354497127336E-4	0.001646645696504404	0.0	0.0752542946847484	0.0
56-57	2.586354497127336E-4	0.0016897516047898597	0.0	0.0827288591814464	0.0
58-59	2.586354497127336E-4	0.0016897516047898597	0.0	0.09010859067991639	0.0
60-61	2.7587781302691587E-4	0.0017414786947324064	0.0	0.0978590329896413	0.0
62-63	2.931201763410981E-4	0.0017759634213607707	0.0	0.10484219013188512	0.0
64-65	2.931201763410981E-4	0.002017356507759322	0.0	0.11117013746818999	0.0
66-67	2.931201763410981E-4	0.0020345988710735044	0.0	0.11804121924889162	0.0
68-69	2.931201763410981E-4	0.0020432200527305956	0.0	0.12468815030650887	0.0
70-71	3.103625396552803E-4	0.0020604624160447775	0.0	0.1314730202706396	0.0
72-73	3.2760490296946255E-4	0.0020690835977018687	0.0	0.13711127307437718	0.0
74-75	3.2760490296946255E-4	0.0021121895059873245	0.0	0.14169774171594968	0.0
76-77	3.448472662836448E-4	0.0021639165959298715	0.0	0.1463014527208363	0.0
78-79	3.6208962959782706E-4	0.0021897801409011446	0.0	0.1501120150132706	0.0
80-81	3.6208962959782706E-4	0.0021897801409011446	0.0	0.15170693361983245	0.0
82-83	3.6208962959782706E-4	0.0022156436858724177	0.0	0.15217247742931536	0.0
84-85	3.6208962959782706E-4	0.002275991957472056	0.0	0.1526207788754841	0.0
86-87	3.6208962959782706E-4	0.0023449614107287848	0.0	0.1529225202334823	0.0
88-89	3.793319929120093E-4	0.0024829003172422426	0.0	0.152991489686739	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5970	0.0	44.1662	1
GTATCAA	10505	0.0	35.465225	1
TCAACGC	12245	0.0	29.30004	4
ATCAACG	12290	0.0	29.192759	3
CAACGCA	12580	0.0	28.552956	5
AACGCAG	13030	0.0	27.726944	6
TATCAAC	13080	0.0	27.718313	2
ACGCAGA	15240	0.0	23.6788	7
CGCAGAG	15510	0.0	23.265995	8
GTACATG	16275	0.0	22.609703	1
TACATGG	16090	0.0	22.377396	2
ACATGGG	16430	0.0	21.328989	3
CATGGGG	12315	0.0	19.682049	4
GCAGAGT	18990	0.0	18.980104	9
GAGTACT	10525	0.0	17.796478	12-13
AGAGTAC	16415	0.0	17.71339	10-11
CAGAGTA	18090	0.0	16.891916	10-11
TATACCG	390	1.10958354E-10	16.045572	5
GTACTTT	11750	0.0	15.906352	14-15
GAGTACA	11815	0.0	15.572278	1
>>END_MODULE