FastQCFastQC Report
Fri 27 May 2016
SRR2140024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8269933
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86750.047.3683241
GTATCAA150350.035.1160771
TCAACGC184000.027.8359814
ATCAACG183900.027.8059773
CAACGCA187450.027.3013555
TATCAAC192000.027.0878662
AACGCAG195400.026.2969446
TACATGG240600.022.8756182
ACGCAGA224500.022.740397
GTACATG248700.022.4308551
CGCAGAG229050.022.2521518
ACATGGG244850.022.0200463
CATGGGG181800.020.1821584
GCAGAGT276700.018.4350369
GAGTACT148200.017.7700512-13
AGAGTAC242100.017.46961410-11
CAGAGTA269650.016.72373410-11
TATACCG6300.016.470475
ATGGGGG120850.016.0389825
GAGTACA172050.015.6571371
Produced by FastQC (version 0.11.5)