##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8269933
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42629668221012	33.0	31.0	34.0	31.0	34.0
2	32.60586403783441	34.0	31.0	34.0	31.0	34.0
3	32.6999148602534	34.0	31.0	34.0	31.0	34.0
4	36.1920270696268	37.0	37.0	37.0	35.0	37.0
5	35.996232617604036	37.0	35.0	37.0	35.0	37.0
6	36.06391043313168	37.0	35.0	37.0	35.0	37.0
7	36.04661017205339	37.0	35.0	37.0	35.0	37.0
8	36.051241769431506	37.0	35.0	37.0	35.0	37.0
9	37.9300817793808	39.0	38.0	39.0	35.0	39.0
10-11	37.77321642146315	39.0	37.5	39.0	35.0	39.0
12-13	37.778017850930596	39.0	38.0	39.0	35.0	39.0
14-15	39.12859342391286	40.0	39.0	41.0	36.0	41.0
16-17	39.09354477236998	40.0	38.5	41.0	36.0	41.0
18-19	39.13122337266819	40.0	39.0	41.0	36.0	41.0
20-21	39.13471897248432	40.0	39.0	41.0	36.0	41.0
22-23	39.070942324337885	40.0	39.0	41.0	36.0	41.0
24-25	39.09380517963152	40.0	39.0	41.0	36.0	41.0
26-27	38.982431595136035	40.0	39.0	41.0	35.5	41.0
28-29	38.90332484478513	40.0	38.0	41.0	35.0	41.0
30-31	38.80382000867765	40.0	38.0	41.0	35.0	41.0
32-33	38.73135055553463	40.0	38.0	41.0	35.0	41.0
34-35	38.67785590545434	40.0	38.0	41.0	35.0	41.0
36-37	38.666862415347396	40.0	38.0	41.0	35.0	41.0
38-39	38.583841159810966	40.0	38.0	41.0	35.0	41.0
40-41	38.55241105563715	40.0	38.0	41.0	35.0	41.0
42-43	38.504182998899566	40.0	38.0	41.0	34.0	41.0
44-45	38.40379245644093	40.0	38.0	41.0	34.0	41.0
46-47	38.33306526055446	40.0	38.0	41.0	34.0	41.0
48-49	38.32815542655226	40.0	38.0	41.0	34.0	41.0
50-51	38.25932761640759	40.0	38.0	41.0	34.0	41.0
52-53	38.072841757160845	40.0	37.0	41.0	33.0	41.0
54-55	37.90395957269885	40.0	37.0	41.0	33.0	41.0
56-57	37.77214132671527	39.0	36.5	41.0	33.0	41.0
58-59	37.60156918151654	39.0	36.0	41.0	33.0	41.0
60-61	37.42226480065527	39.0	36.0	41.0	33.0	41.0
62-63	37.251741627662796	39.0	35.0	40.0	32.5	41.0
64-65	37.03184635349358	38.5	35.0	40.0	32.0	41.0
66-67	36.745485927083934	38.0	35.0	40.0	32.0	41.0
68-69	36.491605680706435	37.0	35.0	40.0	32.0	41.0
70-71	36.2020904673543	37.0	35.0	39.5	31.0	41.0
72-73	35.89930291135147	36.0	35.0	39.0	31.0	41.0
74-75	35.46939725786688	36.0	34.0	39.0	31.0	40.0
76-77	33.55705616258821	34.5	32.0	36.5	28.0	39.0
78-79	34.64356803658502	35.0	34.0	37.0	30.0	39.0
80-81	34.62931369762734	35.0	34.0	37.0	30.5	39.0
82-83	34.3975501434996	35.0	34.0	36.0	30.5	38.5
84-85	34.15162317834918	35.0	34.0	36.0	30.0	37.0
86-87	33.92969293841837	35.0	34.0	35.5	30.0	37.0
88-89	33.77981893983137	35.0	34.0	35.0	30.0	36.5
90-91	33.708816094286966	35.0	34.0	35.0	30.0	36.0
92-93	33.5950280179504	35.0	34.0	35.0	30.0	36.0
94-95	33.501664204829545	35.0	34.0	35.0	30.0	36.0
96-97	33.492834354363225	35.0	33.0	35.0	30.0	35.5
98-99	33.614734724722716	35.0	34.0	35.0	30.5	35.0
100-101	32.38360662066847	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	10.0
20	31.0
21	140.0
22	694.0
23	2224.0
24	5922.0
25	12825.0
26	23552.0
27	38278.0
28	56735.0
29	79430.0
30	106480.0
31	140209.0
32	186133.0
33	254210.0
34	379684.0
35	589581.0
36	1068251.0
37	1973168.0
38	2319528.0
39	1014367.0
40	18476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37104460217513	20.012810260977933	13.197349966438665	24.418795170408274
2	14.980140375367826	21.57823250670956	40.48833659711747	22.95329052080514
3	18.235081227381166	26.453503311332753	30.397307934659207	24.91410752662688
4	12.495453393413031	17.814995192205373	38.99157716945687	30.69797424492473
5	13.015595168667026	38.12761240024557	35.80168061821057	13.055111812876842
6	29.92596191529	38.173864286445855	18.885038125460024	13.01513567280412
7	26.43870803287066	32.59606764222263	23.62840406464721	17.336820260259504
8	24.756165262145846	36.42642308928961	21.531037529974874	17.286374118589677
9	26.047094462203557	16.072441708105945	21.058963967297434	36.82149986239306
10-11	24.310802020406364	26.57596889837091	29.286000996019247	19.82722808520348
12-13	25.078490788211422	24.17373591121413	29.209335109005597	21.538438191568854
14-15	22.165447591454825	25.514943899162386	26.713412326343754	25.60619618303903
16-17	21.875872704954126	28.51749636075043	27.3438251501181	22.26280578417734
18-19	21.893931909726476	27.95560737916498	28.848794784673586	21.301665926434953
20-21	22.80701202796472	27.2640437304902	28.44934229890289	21.479601942642184
22-23	22.466593196945716	27.120129040645686	28.403708558013115	22.009569204395486
24-25	22.133415253165488	27.229537688233705	28.6017614094566	22.035285649144207
26-27	22.124434764802615	27.43431145264175	28.419040602052636	22.022213180502998
28-29	22.06541391597619	27.58717809298904	28.382735398215566	21.964672592819205
30-31	22.3934229109231	27.38831450871146	28.259150906156066	21.959111674209375
32-33	22.13594512726473	27.620152730451466	28.250948292040185	21.992953850243616
34-35	22.208647537493103	27.612471683086227	28.29421445118837	21.884666328232292
36-37	22.22951067178563	27.476586759332537	28.292965394832898	22.000937174048932
38-39	22.05199893362166	27.637879193684366	28.341806422640868	21.9683154500531
40-41	22.30920133939271	27.500330867163992	28.20592498384975	21.984542809593542
42-43	22.201220476959772	27.593604833201084	28.043594561087342	22.1615801287518
44-45	22.309373447666513	27.71673629264082	27.867458967499736	22.106431292192934
46-47	22.356412698825594	27.60339690609935	27.674666929022422	22.365523466052636
48-49	22.280444659905612	27.709316880740488	27.761893633515538	22.248344825838366
50-51	22.2253431333016	27.81534291718784	27.62655964291682	22.332754306593735
52-53	22.434267561484667	27.759399321355176	27.432695705700656	22.373637411459505
54-55	22.41361289702101	27.644707495854714	27.505136979732526	22.43654262739175
56-57	22.39286168739783	27.768498442226768	27.49281673218862	22.345823138186784
58-59	22.333587145680454	27.853623288962908	27.486581833949124	22.32620773140751
60-61	22.413711226684338	27.768624479238834	27.4837770209522	22.333887273124628
62-63	22.408445885616647	27.816769217190107	27.411529492097802	22.36325540509544
64-65	22.352003995437332	27.798627788800484	27.442846597801324	22.406521617960856
66-67	22.48755911259109	27.83232052502434	27.23034245155789	22.449777910826686
68-69	22.39510055813184	27.835206579033063	27.37453376196376	22.395159100871336
70-71	22.378599747671554	27.546601937859933	27.434786355043357	22.64001195942516
72-73	22.467378298154422	27.6285095524252	27.413220142999407	22.49089200642097
74-75	22.26406808428274	27.817440046672594	27.352410785763336	22.566081083281333
76-77	22.408603917386202	27.701789767295033	27.31559876984279	22.57400754547598
78-79	22.440133594136626	27.79644879251964	27.21288228951493	22.550535323828804
80-81	22.38779009651122	27.761964596582533	27.290364613339406	22.55988069356684
82-83	22.451565818582207	27.673215482809255	27.40279307701934	22.4724256215892
84-85	22.563653670615615	27.690325879759726	27.258973479978277	22.487046969646386
86-87	22.32918657490226	27.732012905184934	27.36979454560511	22.569005974307693
88-89	22.41177821011021	27.74309901920863	27.27950626965814	22.56561650102302
90-91	22.47972732510666	27.724086824172172	27.366329982551374	22.429855868169795
92-93	22.52641390501295	27.70865335957657	27.253147777118564	22.51178495829192
94-95	22.339307427967224	27.76443739536523	27.36851704996035	22.5277381267072
96-97	22.346323249648997	27.758957742012964	27.44323616727371	22.451482841064326
98-99	22.5923681878766	28.412302414875164	27.443370675166534	21.551958722081707
100-101	26.719819852561777	35.45313204422636	28.03763298071153	9.78941512250033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1812.0
1	1634.5
2	2257.0
3	3281.5
4	4493.0
5	7038.0
6	9070.0
7	10524.5
8	11493.5
9	11247.5
10	10118.0
11	8978.5
12	9716.5
13	12747.0
14	19314.5
15	27900.5
16	34538.0
17	38381.0
18	39320.5
19	37585.0
20	34985.5
21	33269.5
22	34152.0
23	38681.0
24	46634.0
25	59115.0
26	76571.5
27	94403.0
28	111930.5
29	131466.0
30	148606.0
31	164533.5
32	182680.5
33	201088.5
34	219373.0
35	236647.5
36	256197.5
37	272649.5
38	281373.5
39	289725.0
40	301605.5
41	314122.5
42	322523.0
43	330707.0
44	340711.0
45	349123.0
46	352825.0
47	354164.5
48	354076.5
49	350127.5
50	344614.0
51	336961.5
52	324823.0
53	311455.0
54	296541.0
55	277492.5
56	257669.5
57	235081.0
58	210873.5
59	186428.0
60	158434.5
61	130331.5
62	105182.5
63	83579.0
64	64445.0
65	48721.0
66	36990.5
67	28476.0
68	22176.0
69	16299.5
70	11626.5
71	8424.0
72	5994.0
73	4117.0
74	2781.5
75	1894.0
76	1296.5
77	854.5
78	542.0
79	347.0
80	242.5
81	174.5
82	122.5
83	81.5
84	55.0
85	41.0
86	32.0
87	28.0
88	22.0
89	14.5
90	11.0
91	8.5
92	6.0
93	3.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004727970589362695
3	0.0
4	3.506678953747267E-4
5	0.0
6	0.0
7	0.002261203325347376
8	0.0010882796753008761
9	1.2091996392231957E-5
10-11	1.5114995490289945E-4
12-13	1.632419512951314E-4
14-15	3.627598917669587E-5
16-17	3.3252990078637884E-4
18-19	0.0
20-21	9.069395934093062E-5
22-23	0.0
24-25	0.0
26-27	6.353636509907527E-4
28-29	2.422242826088839E-4
30-31	5.458987747180691E-5
32-33	0.0018431701845871068
34-35	4.88417633708143E-4
36-37	0.0013686113467823028
38-39	0.004045146306880386
40-41	1.747150288682266E-4
42-43	8.877445169498761E-4
44-45	0.0018258671166994378
46-47	1.0945786100924913E-4
48-49	3.8438866045633583E-4
50-51	2.4378626691341353E-4
52-53	0.0
54-55	2.1536536296365888E-4
56-57	0.0016339654578340578
58-59	0.0031831754159090835
60-61	0.0012203216042529115
62-63	0.0010653692209893258
64-65	7.136748086495103E-6
66-67	6.501541009697987E-5
68-69	2.927136117830108E-5
70-71	7.415317628827611E-6
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.212906631487481E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	239.0
20-21	702.0
22-23	1397.0
24-25	3570.0
26-27	5086.0
28-29	10358.0
30-31	22678.0
32-33	27766.0
34-35	37676.0
36-37	58638.0
38-39	71539.0
40-41	70319.0
42-43	78330.0
44-45	92288.0
46-47	92623.0
48-49	87609.0
50-51	81155.0
52-53	78102.0
54-55	84035.0
56-57	87331.0
58-59	86607.0
60-61	84950.0
62-63	80716.0
64-65	82972.0
66-67	88317.0
68-69	89583.0
70-71	88669.0
72-73	84052.0
74-75	84717.0
76-77	83519.0
78-79	87988.0
80-81	89962.0
82-83	85105.0
84-85	83857.0
86-87	87181.0
88-89	92294.0
90-91	97384.0
92-93	100293.0
94-95	108214.0
96-97	158227.0
98-99	661728.0
100-101	4772157.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.74649321049573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.13140589954352	77.28451984017643
2	6.905984038926462	11.843278270111991
3	1.662890035362952	4.277609673811511
4	0.5989277339829487	2.05423811502186
5	0.2891668296037915	1.239752079566104
6	0.14637727326578404	0.7530802720953257
7	0.08483022124893519	0.509172579325667
8	0.05205404218489475	0.3570761259828748
9	0.03242519693956736	0.25023122363048395
>10	0.0940551829128461	1.1934670353212278
>50	0.0014344726540518856	0.07925479319385804
>100	3.406854857997984E-4	0.06058559021429493
>500	6.503280070852739E-5	0.03827224156793752
>1k	4.33550876885606E-5	0.05946215998036672
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2091996392231957E-5	3.627598917669587E-5
2	0.0	0.0	0.0	6.045998196115978E-5	3.627598917669587E-5
3	0.0	0.0	0.0	6.045998196115978E-5	3.627598917669587E-5
4	0.0	1.2091996392231957E-5	0.0	6.045998196115978E-5	4.8367985568927827E-5
5	0.0	1.2091996392231957E-5	0.0	6.045998196115978E-5	6.045998196115978E-5
6	0.0	2.4183992784463913E-5	0.0	6.045998196115978E-5	6.045998196115978E-5
7	0.0	2.4183992784463913E-5	0.0	1.088279675300876E-4	6.045998196115978E-5
8	0.0	2.4183992784463913E-5	0.0	1.2091996392231957E-4	7.255197835339174E-5
9	0.0	4.8367985568927827E-5	0.0	1.6928794949124738E-4	8.464397474562369E-5
10-11	0.0	4.8367985568927827E-5	0.0	2.4183992784463913E-4	8.464397474562369E-5
12-13	0.0	4.8367985568927827E-5	0.0	2.6602392062910306E-4	8.464397474562369E-5
14-15	0.0	6.650598015727576E-5	0.0	3.022999098057989E-4	1.088279675300876E-4
16-17	0.0	7.255197835339174E-5	0.0	3.1439190619803086E-4	1.2091996392231957E-4
18-19	0.0	9.068997294173968E-5	0.0	3.325299007863788E-4	1.3301196031455153E-4
20-21	0.0	9.673597113785565E-5	0.0	3.627598917669587E-4	1.390579585106675E-4
22-23	0.0	1.5114995490289945E-4	0.0	4.171738755320025E-4	1.7533394768736338E-4
24-25	0.0	1.9951794047182728E-4	0.0	4.957718520815103E-4	2.176559350601752E-4
26-27	0.0	2.176559350601752E-4	0.0	6.227378141999457E-4	2.176559350601752E-4
28-29	0.0	2.2370193325629118E-4	0.0	0.0013180276067532833	2.2974793145240718E-4
30-31	0.0	2.78115917021335E-4	0.0	0.003573184933904543	2.3579392964852316E-4
32-33	0.0	2.9020791341356696E-4	0.0	0.007714693698243989	2.539319242368711E-4
34-35	0.0	3.325299007863788E-4	0.0	0.01306540210180663	2.539319242368711E-4
36-37	0.0	3.5066789537472676E-4	0.0	0.020048530018320583	2.7206991882521903E-4
38-39	0.0	3.5066789537472676E-4	0.0	0.031983330457453524	2.9625391160968293E-4
40-41	0.0	3.7485188815919066E-4	0.0	0.043192611113052545	3.1439190619803086E-4
42-43	0.0	3.9903588094365456E-4	0.0	0.05247926434228669	3.2043790439414683E-4
44-45	0.0	4.715878592970463E-4	0.0	0.06318068114941197	3.264839025902628E-4
46-47	0.0	5.320478412582061E-4	0.0	0.07417835186814693	3.264839025902628E-4
48-49	0.0	5.380938394543221E-4	0.0	0.08480721669691882	3.3857589898249476E-4
50-51	0.0	7.194737853378015E-4	0.0	0.0952063335942383	3.3857589898249476E-4
52-53	0.0	7.376117799261494E-4	0.0	0.10520036861241802	3.6880588996307463E-4
54-55	0.0	7.376117799261494E-4	0.0	0.11477118375686962	3.9903588094365456E-4
56-57	0.0	7.617957727106133E-4	0.0	0.12442664287606683	3.9903588094365456E-4
58-59	0.0	7.920257636911932E-4	0.0	0.13325380024239616	3.9903588094365456E-4
60-61	0.0	8.04117760083425E-4	0.0	0.14160332375123233	3.9903588094365456E-4
62-63	0.0	8.22255754671773E-4	0.0	0.15020677918430536	3.9903588094365456E-4
64-65	0.0	8.948077330251647E-4	0.0	0.15774009293666585	3.9903588094365456E-4
66-67	0.0	9.129457276135127E-4	0.0	0.16573290255193118	3.9903588094365456E-4
68-69	0.0	9.250377240057448E-4	0.0	0.17431821999041588	3.9903588094365456E-4
70-71	0.0	9.371297203979766E-4	0.0	0.18201477569407154	4.0508187913977054E-4
72-73	0.0	9.431757185940926E-4	0.0	0.18748640406155648	4.353118701203504E-4
74-75	0.0	9.854977059669044E-4	0.0	0.19220832865272308	4.353118701203504E-4
76-77	0.0	0.0010459576879280643	0.0	0.19616845747117903	4.4740386651258236E-4
78-79	0.0	0.0010520036861241802	0.0	0.19977791839426026	4.4740386651258236E-4
80-81	0.0	0.0010640956825164122	0.0	0.20160380984948728	4.6554186110093034E-4
82-83	0.0	0.0010701416807125282	0.0	0.20226886965106006	4.8367985568927827E-4
84-85	0.0	0.001094325673496992	0.0	0.20273441151216098	4.8367985568927827E-4
86-87	0.0	0.0011124636680853398	0.0	0.20295811344541725	4.8367985568927827E-4
88-89	0.0	0.001136647660869804	0.0	0.20304880341835901	4.897258538853942E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8675	0.0	47.368324	1
GTATCAA	15035	0.0	35.116077	1
TCAACGC	18400	0.0	27.835981	4
ATCAACG	18390	0.0	27.805977	3
CAACGCA	18745	0.0	27.301355	5
TATCAAC	19200	0.0	27.087866	2
AACGCAG	19540	0.0	26.296944	6
TACATGG	24060	0.0	22.875618	2
ACGCAGA	22450	0.0	22.74039	7
GTACATG	24870	0.0	22.430855	1
CGCAGAG	22905	0.0	22.252151	8
ACATGGG	24485	0.0	22.020046	3
CATGGGG	18180	0.0	20.182158	4
GCAGAGT	27670	0.0	18.435036	9
GAGTACT	14820	0.0	17.77005	12-13
AGAGTAC	24210	0.0	17.469614	10-11
CAGAGTA	26965	0.0	16.723734	10-11
TATACCG	630	0.0	16.47047	5
ATGGGGG	12085	0.0	16.038982	5
GAGTACA	17205	0.0	15.657137	1
>>END_MODULE