FastQCFastQC Report
Fri 27 May 2016
SRR2140023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7536362
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68650.043.5739751
GTATCAA120650.034.6905561
TCAACGC137450.029.3966144
ATCAACG138050.029.358633
CAACGCA141100.028.6654555
AACGCAG145800.027.8264086
TATCAAC150400.027.526742
ACGCAGA171500.023.5601437
CGCAGAG173650.023.2920768
GTACATG190150.021.5982781
TACATGG188000.021.2302062
ACATGGG187950.020.8386333
GCAGAGT219200.018.4140199
CATGGGG137200.018.3988324
AGAGTAC189000.017.32341210-11
GAGTACT119300.017.12471212-13
CAGAGTA208450.016.58897410-11
GAGTACA139900.014.94383051
GTACTTT142200.014.46929414-15
ATGGGGG88300.014.2238145
Produced by FastQC (version 0.11.5)