##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7536362
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41798337181786	33.0	31.0	34.0	31.0	34.0
2	32.59875109502436	34.0	31.0	34.0	31.0	34.0
3	32.694919909632794	34.0	31.0	34.0	31.0	34.0
4	36.185844443247284	37.0	35.0	37.0	35.0	37.0
5	35.991604171880276	37.0	35.0	37.0	35.0	37.0
6	36.05722641773312	37.0	35.0	37.0	35.0	37.0
7	36.03719898274525	37.0	35.0	37.0	35.0	37.0
8	36.0385461844853	37.0	35.0	37.0	35.0	37.0
9	37.91656717126911	39.0	38.0	39.0	35.0	39.0
10-11	37.76010600340058	39.0	37.5	39.0	35.0	39.0
12-13	37.76894667480145	39.0	38.0	39.0	35.0	39.0
14-15	39.120762312107615	40.0	38.5	41.0	36.0	41.0
16-17	39.07863827401072	40.0	38.0	41.0	36.0	41.0
18-19	39.11330108612086	40.0	38.5	41.0	36.0	41.0
20-21	39.12081259574944	40.0	39.0	41.0	36.0	41.0
22-23	39.056588084435475	40.0	39.0	41.0	36.0	41.0
24-25	39.08003063293106	40.0	39.0	41.0	36.0	41.0
26-27	38.97337761391727	40.0	39.0	41.0	35.5	41.0
28-29	38.8943099392737	40.0	38.0	41.0	35.0	41.0
30-31	38.79978362961002	40.0	38.0	41.0	35.0	41.0
32-33	38.72850340182699	40.0	38.0	41.0	35.0	41.0
34-35	38.67717477860582	40.0	38.0	41.0	35.0	41.0
36-37	38.66483575392843	40.0	38.0	41.0	35.0	41.0
38-39	38.58364039985716	40.0	38.0	41.0	35.0	41.0
40-41	38.557721413987736	40.0	38.0	41.0	35.0	41.0
42-43	38.5144312692192	40.0	38.0	41.0	34.0	41.0
44-45	38.41890562780904	40.0	38.0	41.0	34.0	41.0
46-47	38.354016236714344	40.0	38.0	41.0	34.0	41.0
48-49	38.35732147500968	40.0	38.0	41.0	34.0	41.0
50-51	38.28914411742957	40.0	38.0	41.0	34.0	41.0
52-53	38.10973857603673	40.0	37.0	41.0	33.5	41.0
54-55	37.9446372095593	40.0	37.0	41.0	33.0	41.0
56-57	37.816721057772185	39.5	37.0	41.0	33.0	41.0
58-59	37.6574873424625	39.0	36.0	41.0	33.0	41.0
60-61	37.47367645727191	39.0	36.0	41.0	33.0	41.0
62-63	37.31621058178367	39.0	35.5	40.5	32.5	41.0
64-65	37.11309941488519	39.0	35.0	40.0	32.0	41.0
66-67	36.83019565490321	38.0	35.0	40.0	32.0	41.0
68-69	36.58770137732296	37.5	35.0	40.0	32.0	41.0
70-71	36.296914184733154	37.0	35.0	39.5	31.0	41.0
72-73	35.99375330425327	36.5	35.0	39.0	31.0	41.0
74-75	35.56010529588549	36.0	34.0	39.0	31.0	40.0
76-77	33.62827231635217	34.5	32.0	36.5	28.0	39.0
78-79	34.71662448495553	35.0	34.0	37.0	30.0	39.0
80-81	34.69153469420104	35.0	34.0	37.0	30.5	39.0
82-83	34.44514619723867	35.0	34.0	36.5	30.5	39.0
84-85	34.182384357147725	35.0	34.0	36.0	30.0	37.0
86-87	33.949287790782634	35.0	34.0	36.0	30.0	37.0
88-89	33.788932907641204	35.0	34.0	35.0	30.0	37.0
90-91	33.71231217258971	35.0	34.0	35.0	30.0	36.0
92-93	33.59713522794532	35.0	34.0	35.0	30.0	36.0
94-95	33.50136249204134	35.0	34.0	35.0	30.0	36.0
96-97	33.48831264668224	35.0	33.0	35.0	30.0	35.5
98-99	33.61368538912329	35.0	34.0	35.0	30.5	35.0
100-101	32.395801995003765	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	12.0
20	35.0
21	152.0
22	576.0
23	2016.0
24	5477.0
25	11783.0
26	21456.0
27	35034.0
28	52283.0
29	72186.0
30	96839.0
31	126720.0
32	168390.0
33	229863.0
34	336177.0
35	521820.0
36	943881.0
37	1762788.0
38	2162846.0
39	967934.0
40	18092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89999100361687	20.394707685220006	13.259859332659445	24.445441978503688
2	14.818489503370339	21.791477166118323	40.63687046164045	22.753162868870884
3	17.979154660982932	26.621150446482062	30.75983223202816	24.639862660506843
4	12.24172286442226	18.162533423085208	39.40426995463178	30.191473757860756
5	12.81467105746778	38.16308186894419	36.24772801518823	12.774519058399795
6	29.536147547052543	38.71282987733339	18.97414163491616	12.776880940697913
7	26.025662256488815	32.69171057471374	23.883642215630044	17.398984953167403
8	24.346199752265207	36.75547036769443	21.79516830905413	17.103161570986238
9	25.49838409968956	16.279114203203775	21.305575267410404	36.91692642969626
10-11	23.796423814011895	26.78158785821206	29.74569397160669	19.676294356169358
12-13	24.59867851183172	24.3035489849731	29.64687689746976	21.450895605725425
14-15	21.7641762540186	25.807765458172355	26.87635140644461	25.55170688136443
16-17	21.630871406558068	28.991778892997427	27.51037540405924	21.86697429638526
18-19	21.647712782374306	28.286466334817778	28.907793972741757	21.15802691006616
20-21	22.31858199462473	27.59797181367183	28.658860397181847	21.424585794521597
22-23	22.01796242010994	27.59981741499688	28.5699080724721	21.81231209242108
24-25	21.832049881681474	27.683774362688485	28.707818845914986	21.776356909715062
26-27	21.801068396641423	27.86603541180416	28.580506575387037	21.752389616167385
28-29	21.6956849129951	27.87472825817887	28.575839268795615	21.853747560030413
30-31	21.953745092849076	27.69586395947629	28.587998777173407	21.762392170501226
32-33	21.721122899265307	27.921154340081443	28.530243353786783	21.827479406866466
34-35	21.818389054439166	28.02560928145369	28.573142101810234	21.58285956229691
36-37	21.852974754426466	27.879825354647647	28.449976308611234	21.817223582314647
38-39	21.722577435728386	27.94474308489603	28.547429706619752	21.785249772755833
40-41	21.955557336228267	27.79191567880494	28.479293459558637	21.773233525408155
42-43	21.888124629796494	27.853099092896226	28.3727985075583	21.885977769748983
44-45	21.886670409504756	28.036592075917078	28.17443538914082	21.902302125437345
46-47	22.050109481656428	27.872725031984857	27.916904638171225	22.16026084818749
48-49	21.92413438412536	27.96484241347428	28.043407934594004	22.067615267806357
50-51	21.856731220361283	28.093292310997107	27.994790806768176	22.05518566187343
52-53	22.02902750485754	27.994129499485176	27.932350588168838	22.04449240748845
54-55	21.993594865215467	27.935041725166126	27.888012311430067	22.183351098188343
56-57	22.08340803609137	28.03680401722052	27.759262329330763	22.120525617357345
58-59	21.93100180675024	28.124886342539952	27.92212333205898	22.021988518650833
60-61	22.085111447449087	28.13547388211574	27.794781758836447	21.984632911598727
62-63	21.975584655930398	28.249578692108653	27.73902678519366	22.03580986676729
64-65	21.930416663349362	28.202607962149134	27.7418794912217	22.12509588327981
66-67	22.039328381727515	28.22264295316686	27.561941418143487	22.17608724696214
68-69	21.99664468138409	28.195452413816795	27.64197716516574	22.16592573963337
70-71	22.01239830357336	27.92919019836908	27.68341912757431	22.374992370483255
72-73	22.056445355298628	27.949623984030893	27.665189266810486	22.328741393859993
74-75	21.948952217720425	28.05997593573754	27.616292678112863	22.37477916842917
76-77	22.06311302177765	27.986814623094787	27.562205281381573	22.38786707374599
78-79	22.072594864942044	28.028450818831317	27.481185732068745	22.417768584157894
80-81	22.066842122932936	28.061094005502035	27.484744251662853	22.387319619902176
82-83	22.09182496168714	28.004211293671037	27.56067554742716	22.343288197214655
84-85	22.193927304183	27.99761042364527	27.48821148058162	22.320250791590116
86-87	22.0744635728234	27.987499682457234	27.565115067869776	22.372921676849586
88-89	22.11743890853718	27.960188092327158	27.569893161860026	22.35247983727563
90-91	22.142351112636653	27.95868328125349	27.608747975811536	22.290217630298322
92-93	22.107039075458857	27.894332216123637	27.628840542654242	22.369788165763264
94-95	21.998287939832363	27.94806443828427	27.72264742857361	22.33100019330976
96-97	22.021757864606283	27.971144896214756	27.760098311017128	22.246998928161833
98-99	22.23089221336286	28.599420820022388	27.7995124407709	21.37017452584385
100-101	26.263660472142497	35.62662228464759	28.44213863825092	9.667578604958994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1325.0
1	1239.5
2	1762.5
3	2534.5
4	3414.0
5	5396.5
6	7076.0
7	8195.0
8	8937.5
9	8878.5
10	8126.0
11	7355.0
12	7921.0
13	10227.0
14	15455.5
15	22357.5
16	27869.5
17	31255.0
18	32535.5
19	32151.0
20	31327.5
21	31197.0
22	33088.0
23	38285.0
24	47428.0
25	60549.5
26	77057.5
27	94905.0
28	114472.5
29	135435.0
30	153181.5
31	169288.0
32	185281.5
33	200450.5
34	215904.5
35	230659.5
36	246827.5
37	261860.5
38	272323.0
39	280312.0
40	290151.5
41	300180.5
42	306320.0
43	311259.5
44	316575.0
45	322597.5
46	329366.5
47	334672.5
48	332450.5
49	325380.5
50	318009.0
51	306031.0
52	289478.5
53	272782.5
54	254841.5
55	234709.0
56	214942.0
57	193640.5
58	171280.5
59	148928.0
60	125771.5
61	103032.5
62	82261.0
63	64405.5
64	49157.5
65	37436.0
66	28672.0
67	21900.0
68	16634.0
69	12128.0
70	8673.0
71	6299.0
72	4550.0
73	3186.5
74	2218.0
75	1539.5
76	1082.0
77	750.0
78	512.0
79	350.5
80	233.0
81	159.5
82	120.0
83	86.0
84	62.5
85	47.0
86	38.5
87	34.5
88	27.5
89	18.5
90	10.0
91	4.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004299156542639539
3	1.3269001674813392E-5
4	3.8480104856958834E-4
5	0.0
6	0.0
7	0.002096502264620516
8	0.001021713128960631
9	7.961401004888035E-5
10-11	1.1942101507332052E-4
12-13	1.3269001674813392E-4
14-15	6.634500837406696E-6
16-17	3.8480104856958834E-4
18-19	0.0
20-21	7.29830197042874E-5
22-23	0.0
24-25	0.0
26-27	6.640084196267609E-4
28-29	2.0600933208983444E-4
30-31	1.9972120251603445E-5
32-33	0.0018832079820237779
34-35	4.559176875479426E-4
36-37	0.0013688020743217722
38-39	0.004018429291860967
40-41	8.232555420877046E-5
42-43	8.448861706330725E-4
44-45	0.0018340503630229687
46-47	8.504248049072345E-5
48-49	2.582261897879289E-4
50-51	2.467430104055156E-4
52-53	0.0
54-55	1.853591619215339E-4
56-57	0.0017403251797598383
58-59	0.003226913521754712
60-61	0.0013223719970815557
62-63	9.809077211061369E-4
64-65	7.88270658721529E-6
66-67	8.789635964441607E-5
68-69	8.102285192822638E-6
70-71	3.285928070541646E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	240.0
20-21	735.0
22-23	1349.0
24-25	3082.0
26-27	4912.0
28-29	10466.0
30-31	22022.0
32-33	27892.0
34-35	37118.0
36-37	57794.0
38-39	66125.0
40-41	67139.0
42-43	73881.0
44-45	86224.0
46-47	85871.0
48-49	82487.0
50-51	76233.0
52-53	70211.0
54-55	77153.0
56-57	80220.0
58-59	80366.0
60-61	83310.0
62-63	78452.0
64-65	83271.0
66-67	87534.0
68-69	84830.0
70-71	82437.0
72-73	77790.0
74-75	78269.0
76-77	77443.0
78-79	81835.0
80-81	82202.0
82-83	78335.0
84-85	77193.0
86-87	80672.0
88-89	84651.0
90-91	88473.0
92-93	91499.0
94-95	98982.0
96-97	144978.0
98-99	599026.0
100-101	4283660.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.90872843789805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.21021659174919	80.18174161125943
2	6.436440081253234	11.316385264193862
3	1.3977971457372051	3.686357090876429
4	0.4662764734348731	1.639590875206682
5	0.20707761452967458	0.9101964890628447
6	0.10360491722412332	0.5464665919851819
7	0.0576490417344821	0.3547497768179147
8	0.03459811586944097	0.24331810979436938
9	0.02114572543733117	0.16730044515834291
>10	0.06410204659498632	0.8436324847674845
>50	8.44505498199844E-4	0.045329260868600396
>100	2.0494192129577376E-4	0.040006058085794424
>500	4.2799015892155845E-5	0.02492594192304912
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.653800334962678E-5	0.0	1.326900167481339E-5	0.0
3	0.0	2.653800334962678E-5	0.0	1.326900167481339E-5	0.0
4	0.0	9.288301172369374E-5	0.0	3.9807005024440174E-5	0.0
5	0.0	9.288301172369374E-5	0.0	3.9807005024440174E-5	0.0
6	3.9807005024440174E-5	9.288301172369374E-5	0.0	3.9807005024440174E-5	1.326900167481339E-5
7	3.9807005024440174E-5	9.288301172369374E-5	0.0	3.9807005024440174E-5	1.326900167481339E-5
8	3.9807005024440174E-5	1.1942101507332053E-4	0.0	3.9807005024440174E-5	1.326900167481339E-5
9	3.9807005024440174E-5	2.2557302847182764E-4	0.0	1.0615201339850713E-4	2.653800334962678E-5
10-11	3.9807005024440174E-5	2.2557302847182764E-4	0.0	1.3269001674813392E-4	2.653800334962678E-5
12-13	3.9807005024440174E-5	2.2557302847182764E-4	0.0	1.592280200977607E-4	2.653800334962678E-5
14-15	3.9807005024440174E-5	3.118215393581147E-4	0.0	1.658625209351674E-4	4.644150586184687E-5
16-17	9.288301172369374E-5	3.184560401955214E-4	0.0	2.0566952595960756E-4	6.634500837406696E-5
18-19	9.288301172369374E-5	3.5162854438255486E-4	0.0	2.3884203014664106E-4	6.634500837406696E-5
20-21	9.288301172369374E-5	3.8480104856958834E-4	0.0	2.919180368458946E-4	6.634500837406696E-5
22-23	9.288301172369374E-5	4.511460569436553E-4	0.0	3.5162854438255486E-4	6.634500837406696E-5
24-25	9.951751256110043E-5	5.042220636429089E-4	0.0	4.57780557781062E-4	6.634500837406696E-5
26-27	1.0615201339850713E-4	5.307600669925357E-4	0.0	5.772015728543825E-4	6.634500837406696E-5
28-29	1.0615201339850713E-4	5.307600669925357E-4	0.0	0.0016055492026524204	6.634500837406696E-5
30-31	1.0615201339850713E-4	5.971050753666026E-4	0.0	0.0051351036481527826	6.634500837406696E-5
32-33	1.0615201339850713E-4	5.971050753666026E-4	0.0	0.011139326906005841	6.634500837406696E-5
34-35	1.0615201339850713E-4	7.165260904399231E-4	0.0	0.018364298317941737	7.961401004888035E-5
36-37	1.0615201339850713E-4	8.226781038384302E-4	0.0	0.028979499657792444	9.288301172369374E-5
38-39	1.0615201339850713E-4	8.425816063506503E-4	0.0	0.0476556195150923	9.951751256110043E-5
40-41	1.0615201339850713E-4	9.15561115562124E-4	0.0	0.08931365027316893	1.0615201339850713E-4
42-43	1.0615201339850713E-4	9.553681205865641E-4	0.0	0.10676238747554855	1.1278651423591383E-4
44-45	1.1278651423591383E-4	0.0011344996431965448	0.0	0.1302816929441553	1.2605551591072722E-4
46-47	1.1942101507332053E-4	0.0012738241607820856	0.0	0.15553923763216257	1.3269001674813392E-4
48-49	1.1942101507332053E-4	0.0012738241607820856	0.0	0.17685588882275027	1.459590184229473E-4
50-51	1.3269001674813392E-4	0.0014529556833920664	0.0	0.20020269726958445	1.459590184229473E-4
52-53	1.3269001674813392E-4	0.0014728591859042865	0.0	0.23180945925898994	1.52593519260354E-4
54-55	1.3269001674813392E-4	0.0014728591859042865	0.0	0.2949898107336139	1.592280200977607E-4
56-57	1.3269001674813392E-4	0.0014993971892539133	0.0	0.3590658198212878	1.592280200977607E-4
58-59	1.3269001674813392E-4	0.0015193006917661334	0.0	0.4268040733712101	1.592280200977607E-4
60-61	1.52593519260354E-4	0.00154583869511576	0.0	0.4731128892163089	1.592280200977607E-4
62-63	1.9903502512220086E-4	0.0015657421976279801	0.0	0.49397839434995294	1.592280200977607E-4
64-65	1.9903502512220086E-4	0.0017117012160509275	0.0	0.5130724877600095	1.592280200977607E-4
66-67	2.1230402679701425E-4	0.0017316047185631477	0.0	0.5223939614365658	1.592280200977607E-4
68-69	2.1230402679701425E-4	0.0017846807252624012	0.0	0.5305875699707632	1.592280200977607E-4
70-71	2.1230402679701425E-4	0.001831122231124248	0.0	0.5380978249187074	1.7249702177257409E-4
72-73	2.6538003349626784E-4	0.0018443912327990614	0.0	0.5440357031681864	1.7249702177257409E-4
74-75	2.6538003349626784E-4	0.001877563736986095	0.0	0.5490646548029408	1.7249702177257409E-4
76-77	2.786490351710812E-4	0.0019505432461975685	0.0	0.5532576593321819	1.7249702177257409E-4
78-79	2.786490351710812E-4	0.0019770812495471953	0.0	0.5571720148262518	1.7249702177257409E-4
80-81	2.786490351710812E-4	0.0019770812495471953	0.0	0.5589036195448148	1.7249702177257409E-4
82-83	2.786490351710812E-4	0.001983715750384602	0.0	0.5595206281226937	1.7249702177257409E-4
84-85	2.786490351710812E-4	0.0020036192528968223	0.0	0.5600911951947107	1.7249702177257409E-4
86-87	2.786490351710812E-4	0.0020965022646205157	0.0	0.5603831132315565	1.7249702177257409E-4
88-89	2.919180368458946E-4	0.0022358267822060567	0.0	0.5604958997457925	1.7249702177257409E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6865	0.0	43.573975	1
GTATCAA	12065	0.0	34.690556	1
TCAACGC	13745	0.0	29.396614	4
ATCAACG	13805	0.0	29.35863	3
CAACGCA	14110	0.0	28.665455	5
AACGCAG	14580	0.0	27.826408	6
TATCAAC	15040	0.0	27.52674	2
ACGCAGA	17150	0.0	23.560143	7
CGCAGAG	17365	0.0	23.292076	8
GTACATG	19015	0.0	21.598278	1
TACATGG	18800	0.0	21.230206	2
ACATGGG	18795	0.0	20.838633	3
GCAGAGT	21920	0.0	18.414019	9
CATGGGG	13720	0.0	18.398832	4
AGAGTAC	18900	0.0	17.323412	10-11
GAGTACT	11930	0.0	17.124712	12-13
CAGAGTA	20845	0.0	16.588974	10-11
GAGTACA	13990	0.0	14.9438305	1
GTACTTT	14220	0.0	14.469294	14-15
ATGGGGG	8830	0.0	14.223814	5
>>END_MODULE