FastQCFastQC Report
Fri 27 May 2016
SRR2140022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13390743
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130500.041.53231
GTATCAA213750.032.4649661
TCAACGC241900.027.9458834
ATCAACG245000.027.6092723
CAACGCA246700.027.4018385
AACGCAG254550.026.6059556
TATCAAC266850.025.6306172
ACGCAGA305100.022.1977927
GTACATG323450.022.0849111
CGCAGAG308100.021.9679748
TACATGG321050.021.614822
ACATGGG321750.020.9975013
CATGGGG245200.018.8099214
TATACCG9250.018.4503615
GCAGAGT389350.017.351479
AGAGTAC334450.016.73362410-11
GAGTACT220150.016.70506712-13
CAGAGTA378150.015.80704610-11
GTACTTT252050.014.67376714-15
ATGGGGG159550.014.5318595
Produced by FastQC (version 0.11.5)