##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13390743
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41780982578786	33.0	31.0	34.0	31.0	34.0
2	32.60349153142585	34.0	31.0	34.0	31.0	34.0
3	32.70005211809382	34.0	31.0	34.0	31.0	34.0
4	36.18892006216533	37.0	35.0	37.0	35.0	37.0
5	35.989773158965114	37.0	35.0	37.0	35.0	37.0
6	36.05325828447309	37.0	35.0	37.0	35.0	37.0
7	36.0373825410584	37.0	35.0	37.0	35.0	37.0
8	36.039753133937374	37.0	35.0	37.0	35.0	37.0
9	37.920330709057744	39.0	38.0	39.0	35.0	39.0
10-11	37.764356652950475	39.0	37.5	39.0	35.0	39.0
12-13	37.7734128718623	39.0	38.0	39.0	35.0	39.0
14-15	39.13021278953677	40.0	39.0	41.0	36.0	41.0
16-17	39.090157917301525	40.0	38.0	41.0	36.0	41.0
18-19	39.11693708855438	40.0	38.5	41.0	36.0	41.0
20-21	39.12466095452025	40.0	39.0	41.0	36.0	41.0
22-23	39.06211951204517	40.0	39.0	41.0	36.0	41.0
24-25	39.08413899342883	40.0	39.0	41.0	36.0	41.0
26-27	38.977746037575216	40.0	39.0	41.0	35.5	41.0
28-29	38.89847408924797	40.0	38.0	41.0	35.0	41.0
30-31	38.79956912511615	40.0	38.0	41.0	35.0	41.0
32-33	38.7292455223715	40.0	38.0	41.0	35.0	41.0
34-35	38.683947759343496	40.0	38.0	41.0	35.0	41.0
36-37	38.66935437184699	40.0	38.0	41.0	35.0	41.0
38-39	38.58842969567168	40.0	38.0	41.0	35.0	41.0
40-41	38.56120054702586	40.0	38.0	41.0	35.0	41.0
42-43	38.5130394571044	40.0	38.0	41.0	34.0	41.0
44-45	38.42226822506227	40.0	38.0	41.0	34.0	41.0
46-47	38.354288501154606	40.0	38.0	41.0	34.0	41.0
48-49	38.35811909896195	40.0	38.0	41.0	34.0	41.0
50-51	38.28657969930215	40.0	38.0	41.0	34.0	41.0
52-53	38.1041831601634	40.0	37.0	41.0	33.5	41.0
54-55	37.936027383251144	40.0	37.0	41.0	33.0	41.0
56-57	37.80717486547819	39.5	37.0	41.0	33.0	41.0
58-59	37.64351207293014	39.0	36.0	41.0	33.0	41.0
60-61	37.45789819124458	39.0	36.0	41.0	33.0	41.0
62-63	37.2936321524417	39.0	35.5	40.0	32.5	41.0
64-65	37.07780432690456	38.5	35.0	40.0	32.0	41.0
66-67	36.78893370128553	38.0	35.0	40.0	32.0	41.0
68-69	36.53808606984971	37.5	35.0	40.0	32.0	41.0
70-71	36.242589323735245	37.0	35.0	39.5	31.0	41.0
72-73	35.9352941945225	36.5	35.0	39.0	31.0	41.0
74-75	35.50364269929511	36.0	34.0	39.0	31.0	40.0
76-77	33.580092647564975	34.5	32.0	36.5	28.0	39.0
78-79	34.665623100433905	35.0	34.0	37.0	30.0	39.0
80-81	34.643996434292376	35.0	34.0	37.0	30.5	39.0
82-83	34.40673801283026	35.0	34.0	36.0	30.5	38.5
84-85	34.14849256879554	35.0	34.0	36.0	30.0	37.0
86-87	33.92158318869052	35.0	34.0	36.0	30.0	37.0
88-89	33.77134007826418	35.0	34.0	35.0	30.0	36.5
90-91	33.6913258293454	35.0	34.0	35.0	30.0	36.0
92-93	33.57563489572223	35.0	34.0	35.0	30.0	36.0
94-95	33.47866117679868	35.0	33.0	35.0	30.0	36.0
96-97	33.46826008013769	35.0	33.0	35.0	30.0	35.5
98-99	33.592101072044485	35.0	34.0	35.0	30.0	35.0
100-101	32.36844645137815	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	17.0
20	56.0
21	274.0
22	1129.0
23	3584.0
24	9536.0
25	20563.0
26	37981.0
27	62870.0
28	92852.0
29	129548.0
30	174931.0
31	229677.0
32	303982.0
33	414691.0
34	606833.0
35	946987.0
36	1703652.0
37	3172948.0
38	3802508.0
39	1646653.0
40	29465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35166786488248	20.04228592842085	13.05383876010465	24.55220744659202
2	15.052573028384478	21.573806270526305	40.372861037408434	23.000759663680785
3	18.4196439599837	26.099576856906065	30.320627490246622	25.160151692863618
4	12.461246352405684	17.796862368775283	39.03676252317377	30.705128755645255
5	13.066212980116191	37.972739824817786	35.98498604595727	12.976061149108753
6	30.178066308797526	38.182275485555614	18.763135007753863	12.876523197892991
7	26.59158842386099	32.33730692899244	23.524938435716432	17.54616621143014
8	24.583846565960307	36.756029260495445	21.442302795077207	17.217821378467036
9	25.68075401359447	16.18221995199668	21.057738407287424	37.079287627121424
10-11	24.131887093653955	26.591402639729818	29.453248858454394	19.823461408161837
12-13	24.905870477732552	24.107261709401907	29.40395420725742	21.58291360560812
14-15	21.969200357859236	25.608790104206342	26.610743693029658	25.811265844904764
16-17	21.886612068583112	28.731204682174244	27.293442121256973	22.088741127985674
18-19	21.920090617824567	28.11228249246513	28.68237781876629	21.28524907094401
20-21	22.568161979133237	27.419147717188565	28.483531301697308	21.529159001980897
22-23	22.285918510287374	27.350476122543597	28.346945326152856	22.01666004101617
24-25	22.104419186457537	27.41769594594443	28.443445241610277	22.03443962598775
26-27	22.10577159698545	27.60452462798805	28.30155666052603	21.98814711450047
28-29	22.00890039947414	27.684712423761308	28.35015732910373	21.956229847660815
30-31	22.29442084439494	27.563353822647922	28.24268607747271	21.89953925548443
32-33	22.030356219206663	27.698088661063274	28.226793012640883	22.044762107089177
34-35	22.135137094209053	27.746739820185596	28.255614570794314	21.862508514811037
36-37	22.178043060046058	27.67637691837982	28.189542560243375	21.95603746133075
38-39	21.981804071760187	27.754061855972758	28.27621775023472	21.987916322032333
40-41	22.244146626314727	27.59359281550741	28.179223275001547	21.983037283176316
42-43	22.1352183447131	27.714120200136932	28.014092916094484	22.136568539055478
44-45	22.188313058595728	27.822827799909238	27.889342734949462	22.099516406545572
46-47	22.25490084552706	27.75363484994796	27.709421061084793	22.28204324344018
48-49	22.19262408620511	27.846456424717296	27.711786090605383	22.249133398472214
50-51	22.154641694927655	27.947485199727538	27.66651570360957	22.231357401735234
52-53	22.35516737819209	27.82196508294808	27.579166949985968	22.24370058887386
54-55	22.28102886023536	27.743618640214823	27.63403311575602	22.341319383793795
56-57	22.313918899037148	27.88095252699317	27.546875221874465	22.258253352095213
58-59	22.262860568738294	27.9191490834098	27.55436463974731	22.263625708104602
60-61	22.332565582713602	27.924300511564184	27.538738972188053	22.204394933534168
62-63	22.30401192022735	27.9897513335589	27.487991530108285	22.218245216105466
64-65	22.266390243589036	27.911311563607804	27.5126992412535	22.309598951549656
66-67	22.34951656426329	27.92120406345048	27.377486946531416	22.35179242575481
68-69	22.315881846345476	27.929528138976835	27.448419024762998	22.30617098991469
70-71	22.33649246034043	27.70145151050172	27.488608014316696	22.47344801484116
72-73	22.33690033616092	27.755795107449554	27.46105986447351	22.44624469191602
74-75	22.199414200990603	27.89724507030225	27.45724030569876	22.44610042300839
76-77	22.336393740987514	27.817667892292853	27.391586262450986	22.45435210426865
78-79	22.324406291501177	27.829453021944566	27.349814786574356	22.4963258999799
80-81	22.289827375631216	27.887229557968894	27.354859649149265	22.468083417250625
82-83	22.35975650882918	27.798006695100003	27.44529021763611	22.396946578434708
84-85	22.42402289498807	27.787554553886228	27.404577868787566	22.383844682338133
86-87	22.30301423132572	27.795791786711792	27.458417109777212	22.442776872185277
88-89	22.349160882007006	27.76355408586434	27.417749788731605	22.46953524339705
90-91	22.377106217860465	27.824229952251656	27.43049743498454	22.36816639490334
92-93	22.376632756405204	27.776738707995165	27.449339083680208	22.397289451919423
94-95	22.29156758903633	27.802782315246066	27.482976955404727	22.42267314031288
96-97	22.308522459141024	27.8325485801979	27.513309280274918	22.345619680386157
98-99	22.540273063333924	28.42979687547284	27.55902701086726	21.470903050325976
100-101	26.66014284834139	35.427751356371225	28.15652359714119	9.755582198146191
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2378.0
1	2162.5
2	2953.5
3	4256.0
4	5762.0
5	9125.0
6	12055.0
7	14096.0
8	15358.5
9	15176.5
10	13900.5
11	12580.5
12	13558.0
13	17601.0
14	26630.0
15	38495.5
16	47911.5
17	53324.5
18	55222.5
19	54664.5
20	53272.5
21	52879.0
22	55411.0
23	63560.0
24	78180.0
25	98631.0
26	125244.5
27	154583.0
28	186070.5
29	219599.0
30	249633.0
31	277650.5
32	305913.5
33	333599.0
34	361363.5
35	387825.5
36	415361.0
37	439983.5
38	460378.5
39	479248.0
40	498131.5
41	517795.0
42	532597.0
43	546296.5
44	559535.0
45	569769.0
46	577426.5
47	582829.0
48	582494.0
49	574922.5
50	564995.5
51	550227.0
52	526745.0
53	501204.0
54	473466.0
55	440799.5
56	407876.5
57	369990.5
58	328651.5
59	286827.5
60	243088.5
61	200423.0
62	161800.5
63	127514.0
64	97000.5
65	73255.0
66	55775.0
67	43034.5
68	33011.5
69	24119.0
70	17426.0
71	12654.0
72	9079.0
73	6392.0
74	4365.5
75	3008.5
76	2119.5
77	1429.5
78	972.0
79	675.0
80	466.5
81	316.5
82	212.0
83	140.5
84	104.0
85	76.0
86	54.5
87	43.0
88	33.0
89	21.5
90	13.5
91	9.5
92	7.0
93	4.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004532982225108793
3	7.467845510887633E-6
4	5.07813494740359E-4
5	0.0
6	7.467845510887633E-6
7	0.0020013825969178857
8	9.633520709045047E-4
9	2.2403536532662898E-5
10-11	1.2321945092964596E-4
12-13	1.381551419514212E-4
14-15	2.2403536532662898E-5
16-17	3.0618166594639296E-4
18-19	0.0
20-21	8.961807130484248E-5
22-23	0.0
24-25	0.0
26-27	6.838803204954672E-4
28-29	1.7952012699253784E-4
30-31	3.371821047116778E-5
32-33	0.0018861813544585645
34-35	4.865043871570234E-4
36-37	0.001395431289702722
38-39	0.004009689458534058
40-41	1.0412431394128538E-4
42-43	9.066064254582556E-4
44-45	0.0018947748948458898
46-47	7.954975475208358E-5
48-49	2.373997086823914E-4
50-51	2.1157296811933073E-4
52-53	0.0
54-55	1.4954220768579652E-4
56-57	0.0017014462587276315
58-59	0.0033494979196132405
60-61	0.0010968974706705747
62-63	0.0011182485147636435
64-65	4.400520018251597E-6
66-67	1.0243593089030763E-4
68-69	1.8041532148251238E-5
70-71	3.197973763823242E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.4791222632108915E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	394.0
20-21	1259.0
22-23	2372.0
24-25	5458.0
26-27	8549.0
28-29	18326.0
30-31	36678.0
32-33	45822.0
34-35	63347.0
36-37	98382.0
38-39	116830.0
40-41	113590.0
42-43	125034.0
44-45	146204.0
46-47	146881.0
48-49	140031.0
50-51	129016.0
52-53	123107.0
54-55	133499.0
56-57	138376.0
58-59	139349.0
60-61	134695.0
62-63	128842.0
64-65	132818.0
66-67	141212.0
68-69	141131.0
70-71	138288.0
72-73	130256.0
74-75	131354.0
76-77	128991.0
78-79	137402.0
80-81	138838.0
82-83	131760.0
84-85	129501.0
86-87	135061.0
88-89	143193.0
90-91	151888.0
92-93	156916.0
94-95	170580.0
96-97	254873.0
98-99	1082353.0
100-101	7818287.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.26185024223994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.35820453993125	75.29487648859133
2	7.313051721125495	12.32422537878463
3	1.8044719999792351	4.561444482856965
4	0.6981587214677073	2.3531258253450265
5	0.31324845907957893	1.3197447373787952
6	0.17804907708823622	0.9001646801626798
7	0.09649518446372464	0.5691603947665779
8	0.05813773781475376	0.3919034685735513
9	0.04331542452607251	0.3284854033135472
>10	0.13336990820979505	1.6952715390945627
>50	0.0029239152619243494	0.1636806559701927
>100	5.279611807996266E-4	0.06830309743980995
>500	3.023596659269203E-5	0.016229093818467273
>1k	1.5113904832029246E-5	0.013384753903940427
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.467845510887633E-6	0.0	7.467845510887633E-6	0.0
3	0.0	2.24035365326629E-5	0.0	7.467845510887633E-6	0.0
4	0.0	1.0454983715242687E-4	0.0	7.467845510887633E-6	7.467845510887633E-6
5	0.0	1.120176826633145E-4	0.0	1.4935691021775266E-5	7.467845510887633E-6
6	0.0	1.120176826633145E-4	0.0	1.4935691021775266E-5	1.4935691021775266E-5
7	0.0	1.120176826633145E-4	0.0	1.4935691021775266E-5	1.4935691021775266E-5
8	0.0	1.1948552817420213E-4	0.0	1.4935691021775266E-5	2.9871382043550532E-5
9	0.0	1.792282922613032E-4	0.0	4.48070730653258E-5	4.48070730653258E-5
10-11	0.0	1.792282922613032E-4	0.0	4.48070730653258E-5	4.48070730653258E-5
12-13	0.0	1.9789790603852228E-4	0.0	4.48070730653258E-5	4.8540995820769615E-5
14-15	0.0	2.725763611473986E-4	0.0	5.227491857621343E-5	7.094453235343251E-5
16-17	0.0	2.800442066582862E-4	0.0	5.227491857621343E-5	1.0081591439698305E-4
18-19	3.7339227554438165E-6	3.0244774319094917E-4	0.0	6.72106095979887E-5	1.344212191959774E-4
20-21	7.467845510887633E-6	3.2111735696816824E-4	0.0	8.96141461306516E-5	1.381551419514212E-4
22-23	7.467845510887633E-6	3.360530479899435E-4	0.0	1.0454983715242687E-4	1.4562298746230884E-4
24-25	7.467845510887633E-6	3.5845658452260637E-4	0.0	1.3068729644053357E-4	1.6429260123952794E-4
26-27	7.467845510887633E-6	3.7339227554438164E-4	0.0	1.8669613777219082E-4	1.6429260123952794E-4
28-29	1.8669613777219083E-5	3.883279665661569E-4	0.0	5.526205678056848E-4	1.6802652399497176E-4
30-31	2.24035365326629E-5	4.3313503963148274E-4	0.0	0.001687733085460605	1.754943695058594E-4
32-33	2.24035365326629E-5	4.3313503963148274E-4	0.0	0.003670446068601272	1.9416398328307846E-4
34-35	2.24035365326629E-5	4.7794211269680856E-4	0.0	0.006138569009949635	2.1656751981574137E-4
36-37	2.24035365326629E-5	5.414187995393535E-4	0.0	0.010268287577470496	2.203014425711852E-4
38-39	2.24035365326629E-5	5.71290181582904E-4	0.0	0.016638359798257646	2.3897105634840425E-4
40-41	2.24035365326629E-5	6.646382504689993E-4	0.0	0.023549850818584152	2.3897105634840425E-4
42-43	2.24035365326629E-5	6.907757097571061E-4	0.0	0.029441980926674494	2.4270497910384807E-4
44-45	2.24035365326629E-5	7.766559331323138E-4	0.0	0.03643935217037621	2.5017282461473576E-4
46-47	2.24035365326629E-5	8.401326199748587E-4	0.0	0.044198443656188455	2.5764067012562335E-4
48-49	2.24035365326629E-5	8.476004654857464E-4	0.0	0.05168495878085331	2.763102839028424E-4
50-51	2.24035365326629E-5	8.96141461306516E-4	0.0	0.05937310573431213	2.763102839028424E-4
52-53	2.24035365326629E-5	8.96141461306516E-4	0.0	0.06624352360432875	3.136495114572806E-4
54-55	2.24035365326629E-5	9.073432295728474E-4	0.0	0.07344252667682444	3.547226617671626E-4
56-57	2.24035365326629E-5	9.185449978391789E-4	0.0	0.08057431913972211	3.584565845226064E-4
58-59	2.24035365326629E-5	9.484163798827294E-4	0.0	0.0874671405462714	3.584565845226064E-4
60-61	2.24035365326629E-5	9.820216846817237E-4	0.0	0.09410978912820595	3.584565845226064E-4
62-63	2.24035365326629E-5	0.0010118930667252744	0.0	0.10041638466215057	3.7339227554438164E-4
64-65	2.24035365326629E-5	0.0011351125176549202	0.0	0.10650641267627943	3.7339227554438164E-4
66-67	2.24035365326629E-5	0.0011537821314321394	0.0	0.11285034743777847	3.7339227554438164E-4
68-69	2.24035365326629E-5	0.0012321945092964594	0.0	0.11879848638720047	3.7339227554438164E-4
70-71	2.24035365326629E-5	0.00126206589134001	0.0	0.12472048787733436	3.808601210552693E-4
72-73	2.24035365326629E-5	0.00126206589134001	0.0	0.12979115497922705	4.406028851423704E-4
74-75	2.24035365326629E-5	0.0012807355051172292	0.0	0.13398061631083502	4.406028851423704E-4
76-77	2.24035365326629E-5	0.0013330104236934427	0.0	0.1379908493501817	4.443368078978142E-4
78-79	2.24035365326629E-5	0.0013554139602261054	0.0	0.14153060812234242	4.48070730653258E-4
80-81	2.24035365326629E-5	0.0013591478829815492	0.0	0.14294949876941107	4.518046534087018E-4
82-83	2.24035365326629E-5	0.0013703496512478806	0.0	0.1435730638695702	4.630064216750333E-4
84-85	2.24035365326629E-5	0.0014039549560468751	0.0	0.144084611287066	4.667403444304771E-4
86-87	2.24035365326629E-5	0.0014487620291122009	0.0	0.14433851803443618	4.704742671859209E-4
88-89	2.24035365326629E-5	0.0014973030249329704	0.0	0.14448040709914303	4.779421126968085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13050	0.0	41.5323	1
GTATCAA	21375	0.0	32.464966	1
TCAACGC	24190	0.0	27.945883	4
ATCAACG	24500	0.0	27.609272	3
CAACGCA	24670	0.0	27.401838	5
AACGCAG	25455	0.0	26.605955	6
TATCAAC	26685	0.0	25.630617	2
ACGCAGA	30510	0.0	22.197792	7
GTACATG	32345	0.0	22.084911	1
CGCAGAG	30810	0.0	21.967974	8
TACATGG	32105	0.0	21.61482	2
ACATGGG	32175	0.0	20.997501	3
CATGGGG	24520	0.0	18.809921	4
TATACCG	925	0.0	18.450361	5
GCAGAGT	38935	0.0	17.35147	9
AGAGTAC	33445	0.0	16.733624	10-11
GAGTACT	22015	0.0	16.705067	12-13
CAGAGTA	37815	0.0	15.807046	10-11
GTACTTT	25205	0.0	14.673767	14-15
ATGGGGG	15955	0.0	14.531859	5
>>END_MODULE