FastQCFastQC Report
Fri 27 May 2016
SRR2140021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7805411
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66000.038.2266121
GTATCAA106850.031.7396371
TCAACGC115550.028.2088284
ATCAACG116250.028.074193
CAACGCA118750.027.3790075
AACGCAG125600.025.9515066
TATCAAC129200.025.485192
GTACATG187650.022.4375951
TACATGG185550.021.7604792
ACGCAGA149500.021.7201527
ACATGGG185300.021.4327983
CGCAGAG152150.021.3414388
CATGGGG131500.019.842364
GCAGAGT197150.016.4906529
TATACCG5650.016.0250175
GAGTACA129000.015.9844351
GAGTACT105300.015.67201912-13
ATGGGGG87150.015.4892615
AGAGTAC167200.015.43263910-11
CAGAGTA188000.015.01672510-11
Produced by FastQC (version 0.11.5)