FastQCFastQC Report
Fri 27 May 2016
SRR2140020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12685143
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146850.046.7948571
GTATCAA250750.033.9823041
TCAACGC301250.027.5240174
ATCAACG305150.027.2131863
CAACGCA306900.026.9901625
TATCAAC316650.026.4498022
AACGCAG315300.026.3110456
GTACATG397050.023.3280121
TACATGG401300.022.6451632
ACGCAGA371000.022.3271667
ACATGGG402850.022.0710053
CGCAGAG379100.021.8169888
CATGGGG315250.019.736184
GAGTACT236650.018.23139812-13
GCAGAGT452800.018.2290849
AGAGTAC401200.017.10145210-11
CAGAGTA442450.016.9330610-11
GTGGTAT76900.016.1390531
GTACTAG30250.015.9705821
GTACTTT273450.015.74067114-15
Produced by FastQC (version 0.11.5)