##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140020_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12685143
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.395393965996284	33.0	31.0	34.0	31.0	34.0
2	32.58897743604467	34.0	31.0	34.0	31.0	34.0
3	32.68409106621817	34.0	31.0	34.0	31.0	34.0
4	36.182543862532725	37.0	35.0	37.0	35.0	37.0
5	35.97371531404888	37.0	35.0	37.0	35.0	37.0
6	36.03820351098919	37.0	35.0	37.0	35.0	37.0
7	36.021863135480615	37.0	35.0	37.0	35.0	37.0
8	36.02693852170212	37.0	35.0	37.0	35.0	37.0
9	37.909464875563486	39.0	38.0	39.0	35.0	39.0
10-11	37.75599108342728	39.0	37.5	39.0	35.0	39.0
12-13	37.760767419019245	39.0	37.5	39.0	35.0	39.0
14-15	39.10171426526291	40.0	38.5	41.0	36.0	41.0
16-17	39.06509733473245	40.0	38.0	41.0	36.0	41.0
18-19	39.098106343775555	40.0	38.5	41.0	36.0	41.0
20-21	39.1041300857611	40.0	39.0	41.0	36.0	41.0
22-23	39.04272373285339	40.0	39.0	41.0	36.0	41.0
24-25	39.060656994677814	40.0	39.0	41.0	36.0	41.0
26-27	38.945794458795774	40.0	38.5	41.0	35.0	41.0
28-29	38.86559844497253	40.0	38.0	41.0	35.0	41.0
30-31	38.7650575776578	40.0	38.0	41.0	35.0	41.0
32-33	38.686704529337185	40.0	38.0	41.0	35.0	41.0
34-35	38.63654031280852	40.0	38.0	41.0	35.0	41.0
36-37	38.61794097588938	40.0	38.0	41.0	35.0	41.0
38-39	38.53389652897202	40.0	38.0	41.0	34.5	41.0
40-41	38.49771551591225	40.0	38.0	41.0	34.0	41.0
42-43	38.44282891737944	40.0	38.0	41.0	34.0	41.0
44-45	38.33994580184455	40.0	38.0	41.0	34.0	41.0
46-47	38.26048064421669	40.0	38.0	41.0	34.0	41.0
48-49	38.253623017421724	40.0	38.0	41.0	34.0	41.0
50-51	38.17159046525411	40.0	37.0	41.0	34.0	41.0
52-53	37.97445870793962	40.0	37.0	41.0	33.0	41.0
54-55	37.79115673234497	39.5	36.5	41.0	33.0	41.0
56-57	37.6457775871249	39.0	36.0	41.0	33.0	41.0
58-59	37.46255882115925	39.0	36.0	41.0	33.0	41.0
60-61	37.260688493954774	39.0	35.0	40.0	32.0	41.0
62-63	37.0746070812775	39.0	35.0	40.0	32.0	41.0
64-65	36.840684831866184	38.0	35.0	40.0	32.0	41.0
66-67	36.53666300691465	37.5	35.0	40.0	31.5	41.0
68-69	36.27745500720519	37.0	35.0	40.0	31.0	41.0
70-71	35.98343391357686	36.5	35.0	39.0	31.0	41.0
72-73	35.68297355451929	36.0	34.0	39.0	31.0	40.5
74-75	35.25553362070991	35.5	34.0	38.5	30.5	40.0
76-77	33.36869020903606	34.5	32.0	36.0	27.5	39.0
78-79	34.44355958521808	35.0	34.0	37.0	30.0	39.0
80-81	34.44285248013237	35.0	34.0	36.5	30.0	39.0
82-83	34.23212002512996	35.0	34.0	36.0	30.0	37.5
84-85	33.99976024212158	35.0	34.0	36.0	30.0	37.0
86-87	33.79513695058477	35.0	34.0	35.0	30.0	37.0
88-89	33.66391163444918	35.0	34.0	35.0	30.0	36.0
90-91	33.60796537118298	35.0	34.0	35.0	30.0	36.0
92-93	33.50418465535242	35.0	33.5	35.0	30.0	36.0
94-95	33.421363257904744	35.0	33.0	35.0	30.0	36.0
96-97	33.418321053096214	35.0	33.0	35.0	30.0	35.0
98-99	33.550766854628165	35.0	33.0	35.0	30.0	35.0
100-101	32.31814122529893	34.0	31.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	13.0
20	51.0
21	244.0
22	1132.0
23	3687.0
24	9646.0
25	20711.0
26	38550.0
27	62234.0
28	92437.0
29	129220.0
30	171188.0
31	224730.0
32	298117.0
33	410798.0
34	610864.0
35	954018.0
36	1723180.0
37	3111383.0
38	3422244.0
39	1380971.0
40	19715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.894337099707904	19.752903061479085	13.193576138637145	24.159183700175866
2	15.26320602622876	21.16871311950365	40.25218868623641	23.315892168031187
3	18.629207087978532	26.15453987894497	29.925495501035073	25.29075753204143
4	12.877961210863328	17.403349978060874	38.22944056088491	31.489248250190894
5	13.36710985441788	37.928858980935416	35.32846259596758	13.375568568679123
6	30.780023528311823	37.3777260532262	18.448440037294024	13.393810381167953
7	27.075019637534165	32.25094860878595	23.090381849526192	17.5836499041537
8	25.283340179993452	36.19940753725439	20.939293971777886	17.577958310974278
9	26.080585150768133	16.192449102457747	20.807486491478492	36.91947925529562
10-11	24.82772144539372	26.28686749243701	28.660675397365026	20.22473566480425
12-13	25.520124990500687	23.838324323830022	28.850072730861754	21.791477954807537
14-15	22.55845244447815	25.186389335364893	26.21186473213029	26.04329348802666
16-17	22.31198547397785	28.365455147879437	26.798186880242227	22.524372497900487
18-19	22.365069120624025	27.65122947372371	28.376909113283155	21.60679229236911
20-21	23.078328179879207	26.874336618344174	28.173552604551627	21.87378259722499
22-23	22.928659819500037	26.704068128427544	28.100587356171854	22.266684695900572
24-25	22.607340453412725	26.878268157689117	28.145225548969254	22.369165839928897
26-27	22.554149448488964	27.059690046563993	28.06810379210961	22.318056712837432
28-29	22.48190903893761	27.15968732653064	28.034705362013103	22.323698272518648
30-31	22.753129135668804	27.007815412817127	27.901968318280083	22.337087133233986
32-33	22.5109935936293	27.221192278079524	27.90864891781019	22.359165210480985
34-35	22.611424091097547	27.192473840118588	27.954030015556146	22.242072053227716
36-37	22.609486762455084	27.138717874981598	27.928923915781255	22.32287144678206
38-39	22.491023128254586	27.25288890441149	27.968171175072655	22.28791679226127
40-41	22.697584380371165	27.05887967321378	27.854787229433153	22.3887487169819
42-43	22.57185984069145	27.1877093924953	27.675935261335997	22.564495505477254
44-45	22.62911020871379	27.292191884454358	27.54327617617299	22.535421730658854
46-47	22.77200898501229	27.244153780901716	27.32012276735928	22.663714466726713
48-49	22.66681909583748	27.417451368415474	27.253945187307888	22.66178434843916
50-51	22.613994635708163	27.50086003638639	27.19235444608549	22.692790881819967
52-53	22.805824855306806	27.33258789157379	27.091562686505018	22.77002456661439
54-55	22.776925315304858	27.22390501951199	27.16515061550595	22.834019049677202
56-57	22.727979691775154	27.37269096604273	27.158733424868892	22.74059591731322
58-59	22.73794086384487	27.473351869851186	27.074452733066927	22.714254533237018
60-61	22.796860027157027	27.42148379135522	27.105289536148536	22.676366645339215
62-63	22.77877168159998	27.448508736189925	27.033989860071856	22.73872972213824
64-65	22.779286816818626	27.474494407542515	26.97561846136731	22.770600314271544
66-67	22.833666695331203	27.416751011362688	26.886900960024413	22.862681333281692
68-69	22.71922321470105	27.439705249927137	27.011321790031396	22.82974974534042
70-71	22.74785552798912	27.218439668786793	27.037184115714357	22.996520687509726
72-73	22.807831310083788	27.319906632954954	26.989008194901686	22.88325386205957
74-75	22.62898424520522	27.506338828010513	27.001005927043714	22.863670999740553
76-77	22.84662793041997	27.34557142612795	26.917988009712268	22.88981263373981
78-79	22.78791366941349	27.37815776718308	26.889827910534663	22.944100652868762
80-81	22.787996626154385	27.415381866122278	26.91329153315614	22.8833299745672
82-83	22.762847884522373	27.375573297095297	27.003154835923894	22.858423982458437
84-85	22.86148273234754	27.37715823653527	26.93529006475202	22.82606896636517
86-87	22.722315000795877	27.382203300953407	27.053193975597665	22.842287722653047
88-89	22.754149379833276	27.35149877763176	26.96037764938221	22.93397419315275
90-91	22.845224334685778	27.352156474531153	27.00312722839136	22.799491962391713
92-93	22.838664830056977	27.360707209844172	26.95542396775647	22.845203992342388
94-95	22.708621920350097	27.382326200062973	27.04115032683294	22.86790155275399
96-97	22.718974821547466	27.404731765388956	27.04947997566102	22.82681343740256
98-99	22.970849640585485	28.020263302233843	27.060983416516056	21.94790364066462
100-101	27.283455849653183	34.95603255075721	27.654778637563403	10.105732962026206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3112.0
1	2754.0
2	3675.5
3	5249.0
4	6841.0
5	10711.5
6	14182.5
7	16643.5
8	18461.0
9	18278.0
10	16321.5
11	14260.5
12	14830.0
13	18928.0
14	28751.0
15	42002.5
16	52513.5
17	58550.0
18	60115.5
19	57661.0
20	53538.5
21	50051.5
22	49206.0
23	53378.0
24	64006.5
25	81227.0
26	103713.5
27	127622.0
28	153783.0
29	183976.0
30	211836.0
31	238033.5
32	265437.5
33	290458.0
34	311897.0
35	333300.5
36	357857.0
37	382551.0
38	403718.5
39	417990.0
40	431670.0
41	448729.5
42	462454.5
43	476708.0
44	494441.5
45	512552.5
46	526706.0
47	535926.0
48	541470.0
49	540146.5
50	536566.0
51	532290.0
52	520922.5
53	507916.0
54	495283.0
55	471541.0
56	441339.5
57	404949.0
58	363439.0
59	322048.0
60	275303.0
61	227627.5
62	183799.5
63	145362.0
64	111260.0
65	84035.0
66	64514.5
67	49978.5
68	38634.5
69	28236.5
70	19751.0
71	14018.5
72	9991.0
73	6975.5
74	4854.0
75	3353.5
76	2290.0
77	1527.0
78	1040.0
79	712.5
80	479.5
81	322.5
82	224.5
83	157.5
84	108.5
85	82.5
86	67.5
87	50.0
88	35.5
89	24.5
90	17.0
91	13.0
92	9.5
93	6.5
94	3.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0046747600716838585
3	2.3649713684741276E-5
4	3.547457052711191E-4
5	0.0
6	0.0
7	0.0019786927116233533
8	9.932879747591335E-4
9	1.5766475789827517E-5
10-11	1.4189828210844766E-4
12-13	1.497815200033614E-4
14-15	1.5766475789827517E-5
16-17	3.626289431660329E-4
18-19	0.0
20-21	9.066150258747929E-5
22-23	0.0
24-25	0.0
26-27	6.43106810335413E-4
28-29	1.5794595705338865E-4
30-31	3.9554471095016486E-5
32-33	0.0018763973060647184
34-35	5.533840209209417E-4
36-37	0.001500665034716785
38-39	0.003944126646955174
40-41	1.4239349800368383E-4
42-43	9.236121065597149E-4
44-45	0.0019284772975505268
46-47	7.973317084552412E-5
48-49	2.2074729070906916E-4
50-51	2.1892567846677503E-4
52-53	0.0
54-55	2.4099369323886497E-4
56-57	0.0017763400891253169
58-59	0.003338200384686147
60-61	0.001110713772778246
62-63	0.0011325148262009276
64-65	9.270546149195905E-6
66-67	1.2191009486856028E-4
68-69	4.746683504102393E-6
70-71	7.691313368190046E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	2.074696436900779E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	413.0
20-21	1132.0
22-23	2340.0
24-25	6560.0
26-27	8606.0
28-29	17124.0
30-31	35234.0
32-33	41984.0
34-35	56831.0
36-37	89206.0
38-39	109256.0
40-41	108128.0
42-43	118231.0
44-45	139524.0
46-47	138839.0
48-49	132975.0
50-51	122420.0
52-53	114827.0
54-55	122603.0
56-57	127991.0
58-59	129496.0
60-61	126561.0
62-63	118379.0
64-65	120883.0
66-67	129235.0
68-69	131849.0
70-71	130881.0
72-73	122956.0
74-75	124179.0
76-77	122060.0
78-79	130800.0
80-81	131912.0
82-83	125145.0
84-85	122890.0
86-87	128173.0
88-89	135962.0
90-91	145905.0
92-93	151739.0
94-95	163571.0
96-97	243525.0
98-99	1029425.0
100-101	7425393.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.70188001000376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.14333577510962	72.0147624317948
2	7.752510308477986	12.667893339991714
3	2.134499930868316	5.231779716994935
4	0.85412327429377	2.791339090804045
5	0.42446084032443565	1.7339624322566205
6	0.23147216158576084	1.134702646292162
7	0.13988094906819004	0.7999975561518297
8	0.08094457552996068	0.5290659197927627
9	0.05215575813937771	0.3835101144000793
>10	0.1807194185321134	2.260786759408039
>50	0.004421895508249427	0.23907326482000077
>100	0.00141874844365097	0.17470526612393442
>500	3.757623439292445E-5	0.022257853703354813
>1k	1.878788416059729E-5	0.016163607465695114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.1532951579655034E-5	0.0	0.0	0.0
3	0.0	3.1532951579655034E-5	0.0	0.0	0.0
4	0.0	1.0248209263387887E-4	0.0	0.0	0.0
5	0.0	1.0248209263387887E-4	0.0	0.0	0.0
6	0.0	1.0248209263387887E-4	0.0	0.0	0.0
7	0.0	1.1036533052879261E-4	0.0	0.0	0.0
8	0.0	1.2613180631862014E-4	0.0	0.0	0.0
9	0.0	2.680300884270678E-4	0.0	0.0	7.883237894913759E-6
10-11	0.0	2.680300884270678E-4	0.0	3.941618947456879E-6	7.883237894913759E-6
12-13	0.0	2.680300884270678E-4	0.0	7.883237894913759E-6	7.883237894913759E-6
14-15	0.0	4.3357808422025675E-4	0.0	7.883237894913759E-6	1.1824856842370638E-5
16-17	0.0	4.57227797904998E-4	0.0	7.883237894913759E-6	1.5766475789827517E-5
18-19	0.0	4.848191305371961E-4	0.0	7.883237894913759E-6	1.5766475789827517E-5
20-21	0.0	5.124104631693943E-4	0.0	1.5766475789827517E-5	1.5766475789827517E-5
22-23	0.0	5.873012231710751E-4	0.0	3.1532951579655034E-5	2.3649713684741276E-5
24-25	0.0	6.661336021202126E-4	0.0	7.094914105422383E-5	3.5474570527111915E-5
26-27	0.0	7.134330294896952E-4	0.0	1.340150442135339E-4	4.729942736948255E-5
28-29	0.0	7.173746484371521E-4	0.0	5.439434147490493E-4	5.1241046316939426E-5
30-31	0.0	8.198567410710309E-4	0.0	0.0019313932842538709	5.518266526439631E-5
32-33	0.0	8.277399789659446E-4	0.0	0.004584102835892351	7.094914105422383E-5
34-35	0.0	9.972295937065905E-4	0.0	0.007954187035967984	7.094914105422383E-5
36-37	0.0	0.0011588359705523225	0.0	0.012640771964494212	7.094914105422383E-5
38-39	0.0	0.0011627775894997795	0.0	0.020228388438348704	8.277399789659446E-5
40-41	0.0	0.0013165007284505978	0.0	0.029104914308021597	8.671561684405134E-5
42-43	0.0	0.0013440920610827958	0.0	0.037098517533464145	9.065723579150822E-5
44-45	0.0	0.0014859903431912434	0.0	0.045553290175759154	9.45988547389651E-5
46-47	0.0	0.0015924140547725792	0.0	0.05501711726860312	1.0248209263387887E-4
48-49	0.0	0.0016042389116149498	0.0	0.06449671083723692	1.0248209263387887E-4
50-51	0.0	0.00179343662109288	0.0	0.07366885812796908	1.0248209263387887E-4
52-53	0.0	0.0017973782400403369	0.0	0.08261633313869618	1.1824856842370638E-4
54-55	3.941618947456879E-6	0.0018052614779352507	0.0	0.09140614339152503	1.4583990105590454E-4
56-57	7.883237894913759E-6	0.0018367944295149057	0.0	0.0992144905264371	1.5372313895081828E-4
58-59	7.883237894913759E-6	0.0018407360484623625	0.0	0.1071489694676678	1.6160637684573204E-4
60-61	7.883237894913759E-6	0.0018801522379369314	0.0	0.11470111137099519	1.6554799579318892E-4
62-63	7.883237894913759E-6	0.0019038019516216726	0.0	0.1217290179543108	1.6554799579318892E-4
64-65	7.883237894913759E-6	0.0020811748042572323	0.0	0.1288672898681552	1.6554799579318892E-4
66-67	7.883237894913759E-6	0.0021402990884690854	0.0	0.13547344322409294	1.694896147406458E-4
68-69	7.883237894913759E-6	0.0022073066105758525	0.0	0.14198499772529172	1.7343123368810268E-4
70-71	7.883237894913759E-6	0.0022427811811029643	0.0	0.14832312099280237	1.891977094779302E-4
72-73	7.883237894913759E-6	0.0022467228000504214	0.0	0.15343540076765394	2.0496418526775773E-4
74-75	7.883237894913759E-6	0.002290080608472447	0.0	0.15772782380143446	2.0496418526775773E-4
76-77	7.883237894913759E-6	0.0023728546063690415	0.0	0.16145265370678125	2.0496418526775773E-4
78-79	7.883237894913759E-6	0.00241227079584361	0.0	0.16478726333632976	2.0496418526775773E-4
80-81	7.883237894913759E-6	0.002416212414791067	0.0	0.16636785253425995	2.1284742316267147E-4
82-83	7.883237894913759E-6	0.002420154033738524	0.0	0.16687237975953445	2.4832199368978337E-4
84-85	7.883237894913759E-6	0.0024595702232130924	0.0	0.16732566593849196	2.522636126372403E-4
86-87	7.883237894913759E-6	0.0024911031747927477	0.0	0.1675976376458665	2.562052315846972E-4
88-89	7.883237894913759E-6	0.0026054101242689973	0.0	0.16769223650060547	2.6014685053215404E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14685	0.0	46.794857	1
GTATCAA	25075	0.0	33.982304	1
TCAACGC	30125	0.0	27.524017	4
ATCAACG	30515	0.0	27.213186	3
CAACGCA	30690	0.0	26.990162	5
TATCAAC	31665	0.0	26.449802	2
AACGCAG	31530	0.0	26.311045	6
GTACATG	39705	0.0	23.328012	1
TACATGG	40130	0.0	22.645163	2
ACGCAGA	37100	0.0	22.327166	7
ACATGGG	40285	0.0	22.071005	3
CGCAGAG	37910	0.0	21.816988	8
CATGGGG	31525	0.0	19.73618	4
GAGTACT	23665	0.0	18.231398	12-13
GCAGAGT	45280	0.0	18.229084	9
AGAGTAC	40120	0.0	17.101452	10-11
CAGAGTA	44245	0.0	16.93306	10-11
GTGGTAT	7690	0.0	16.139053	1
GTACTAG	3025	0.0	15.970582	1
GTACTTT	27345	0.0	15.740671	14-15
>>END_MODULE