FastQCFastQC Report
Fri 27 May 2016
SRR2140019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16177360
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161750.044.219541
GTATCAA286650.035.1032871
TCAACGC335450.029.0580964
ATCAACG337100.028.9648913
CAACGCA341000.028.5607935
AACGCAG354050.027.6135356
TATCAAC362900.027.2147032
ACGCAGA421150.023.1746797
GTACATG483400.022.922211
CGCAGAG426650.022.8848538
TACATGG475900.022.6662062
ACATGGG482700.021.848663
CATGGGG340150.019.8223274
GCAGAGT522350.018.6523179
GAGTACT274500.017.51314412-13
AGAGTAC461700.017.25676710-11
CAGAGTA504250.016.60498810-11
GAGTACA339900.015.8065941
GTGGTAT95600.015.6734821
ATGGGGG222800.015.5122215
Produced by FastQC (version 0.11.5)