##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16177360
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42309041771958	33.0	31.0	34.0	31.0	34.0
2	32.6085967673341	34.0	31.0	34.0	31.0	34.0
3	32.704800597872584	34.0	31.0	34.0	31.0	34.0
4	36.19107171998398	37.0	37.0	37.0	35.0	37.0
5	35.9973117368965	37.0	35.0	37.0	35.0	37.0
6	36.06256954163102	37.0	35.0	37.0	35.0	37.0
7	36.04675008777699	37.0	35.0	37.0	35.0	37.0
8	36.048201931588345	37.0	35.0	37.0	35.0	37.0
9	37.926048255092304	39.0	38.0	39.0	35.0	39.0
10-11	37.77384214729721	39.0	37.5	39.0	35.0	39.0
12-13	37.78153821142634	39.0	38.0	39.0	35.0	39.0
14-15	39.13319314770766	40.0	39.0	41.0	36.0	41.0
16-17	39.09345622524318	40.0	38.0	41.0	36.0	41.0
18-19	39.125629583566166	40.0	38.5	41.0	36.0	41.0
20-21	39.13489510325927	40.0	39.0	41.0	36.0	41.0
22-23	39.072536726719434	40.0	39.0	41.0	36.0	41.0
24-25	39.09442990006857	40.0	39.0	41.0	36.0	41.0
26-27	38.9869977960311	40.0	39.0	41.0	35.5	41.0
28-29	38.906847130991764	40.0	38.0	41.0	35.0	41.0
30-31	38.80592924747799	40.0	38.0	41.0	35.0	41.0
32-33	38.73214488229118	40.0	38.0	41.0	35.0	41.0
34-35	38.68436885334826	40.0	38.0	41.0	35.0	41.0
36-37	38.66915429015413	40.0	38.0	41.0	35.0	41.0
38-39	38.59070605432042	40.0	38.0	41.0	35.0	41.0
40-41	38.56297933476927	40.0	38.0	41.0	35.0	41.0
42-43	38.51464411187894	40.0	38.0	41.0	34.0	41.0
44-45	38.42420433934493	40.0	38.0	41.0	34.0	41.0
46-47	38.352825619224035	40.0	38.0	41.0	34.0	41.0
48-49	38.354167863337366	40.0	38.0	41.0	34.0	41.0
50-51	38.284434404914506	40.0	38.0	41.0	34.0	41.0
52-53	38.10261351840155	40.0	37.0	41.0	33.5	41.0
54-55	37.93458004434717	40.0	37.0	41.0	33.0	41.0
56-57	37.80972695238893	39.5	37.0	41.0	33.0	41.0
58-59	37.64555533062355	39.0	36.0	41.0	33.0	41.0
60-61	37.464219829702174	39.0	36.0	41.0	33.0	41.0
62-63	37.29914716735716	39.0	35.5	40.0	32.5	41.0
64-65	37.08515711680943	38.5	35.0	40.0	32.0	41.0
66-67	36.79655882854641	38.0	35.0	40.0	32.0	41.0
68-69	36.54789199477584	37.5	35.0	40.0	32.0	41.0
70-71	36.25679486040809	37.0	35.0	39.5	31.0	41.0
72-73	35.95142637624454	36.5	35.0	39.0	31.0	41.0
74-75	35.5224723280311	36.0	34.0	39.0	31.0	40.0
76-77	33.59438402657803	34.5	32.0	36.5	28.0	39.0
78-79	34.68638223195357	35.0	34.0	37.0	30.0	39.0
80-81	34.66721603515265	35.0	34.0	37.0	31.0	39.0
82-83	34.42896694172006	35.0	34.0	36.0	30.5	38.5
84-85	34.17148351493788	35.0	34.0	36.0	30.0	37.0
86-87	33.94485461005932	35.0	34.0	36.0	30.0	37.0
88-89	33.79216893696751	35.0	34.0	35.0	30.0	36.5
90-91	33.711866936313456	35.0	34.0	35.0	30.0	36.0
92-93	33.59516370075971	35.0	34.0	35.0	30.0	36.0
94-95	33.49848962716179	35.0	33.5	35.0	30.0	36.0
96-97	33.48804118693436	35.0	33.0	35.0	30.0	35.5
98-99	33.60946131048837	35.0	34.0	35.0	30.5	35.0
100-101	32.38162002297213	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	30.0
20	73.0
21	324.0
22	1236.0
23	4124.0
24	11202.0
25	24323.0
26	44680.0
27	73953.0
28	109516.0
29	153986.0
30	206940.0
31	272648.0
32	360795.0
33	496786.0
34	731365.0
35	1138717.0
36	2058977.0
37	3820194.0
38	4605577.0
39	2026420.0
40	35491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25148602738642	20.124958584095303	13.294709396341553	24.32884599217672
2	14.930549481118591	21.5259363433349	40.6034386035406	22.940075572005902
3	18.398980847181598	26.301482603030728	30.200419623525672	25.099116926262
4	12.468759424869907	17.80130638780477	38.98019561880586	30.749738568519465
5	13.074191339007108	38.06672411320512	35.77309276668134	13.085991781106435
6	30.022834380887858	38.0882727466039	18.838698032311825	13.050194840196422
7	26.521994591719317	32.49416688894665	23.534489766208846	17.449348753125186
8	24.588746740241834	36.581689646234764	21.50289694809305	17.32666666543036
9	25.840438791191612	16.294161350129407	21.148367103073042	36.71703275560594
10-11	24.207709672912852	26.713044295292054	29.186983150505586	19.892262881289508
12-13	24.949453224471203	24.255646438838767	29.263190168595365	21.531710168094666
14-15	22.02382621012925	25.642897995824722	26.698701162628435	25.634574631417596
16-17	21.758557816332967	28.75937621611367	27.384146855394885	22.097919112158475
18-19	21.80949796505734	28.125318346133117	28.84995141358046	21.215232275229084
20-21	22.571042433749184	27.423868452673023	28.604990978530058	21.400098135047735
22-23	22.31086198623365	27.33679997499849	28.435330267433045	21.917007771334816
24-25	22.017108205992542	27.405616145735678	28.588634534357897	21.988641113913882
26-27	21.995914383058047	27.61749180040151	28.477211590254726	21.90938222628572
28-29	21.91569913919116	27.769874680848574	28.438246996659792	21.876179183300472
30-31	22.19879200629481	27.5680594476004	28.361874846266016	21.87127369983877
32-33	21.940455134772996	27.730661859634374	28.338470368126313	21.990412637466317
34-35	22.062642139406588	27.749245291505115	28.375978185515322	21.812134383572978
36-37	22.063390022257668	27.666328002843205	28.33666897585706	21.933612999042065
38-39	21.909058282844043	27.812173388806695	28.376872634115358	21.9018956942339
40-41	22.154874074403228	27.659795885090205	28.270944174787964	21.914385865718604
42-43	22.042368446809665	27.748978206221697	28.114885117910532	22.0937682290581
44-45	22.130240128039976	27.875333354674197	27.95593148331917	22.038495033966658
46-47	22.181396413427642	27.81103281219439	27.774647601304064	22.2329231730739
48-49	22.13607895828411	27.909870282695024	27.759397821858155	22.194652937162708
50-51	22.122405559004164	28.008183401579544	27.67967463532023	22.18973640409606
52-53	22.300998426166917	27.880223392710768	27.57871366358873	22.24006451753359
54-55	22.214187439374736	27.833595196876505	27.642737192809708	22.309480170939054
56-57	22.247971344593275	27.93306244448242	27.599257886267527	22.21970832465678
58-59	22.205939958704604	28.00802467671857	27.56509181508352	22.22094354949331
60-61	22.30409209480336	27.92939569226735	27.58718360107406	22.179328611855237
62-63	22.231678915132438	27.966623081687423	27.560793801998628	22.240904201181504
64-65	22.195850597831072	27.916310760299563	27.595648683197144	22.292189958672225
66-67	22.30040333557081	27.971131266075705	27.422046227813546	22.30641917053994
68-69	22.274041569379257	27.958346385420974	27.468961728207965	22.298650316991804
70-71	22.21787270475754	27.776384483620596	27.544518226136404	22.46122458548546
72-73	22.262251412469976	27.81878954015779	27.552166080909444	22.366792966462793
74-75	22.157815227345665	27.948824721933047	27.481253473390893	22.412106577330402
76-77	22.244967601384104	27.868218860338857	27.441682193957057	22.445131344319986
78-79	22.246394384940594	27.91449051881085	27.38774505920123	22.451370037047322
80-81	22.217452120077063	27.92442217176462	27.407424292457428	22.45070141570089
82-83	22.27548050881441	27.872967754344014	27.50407800917444	22.347473727667136
84-85	22.34731979235585	27.856708570625983	27.404393155265733	22.391578481752436
86-87	22.23777433557795	27.86452724577372	27.474505657944597	22.423192760703735
88-89	22.272531173148483	27.868337584539503	27.445737789887897	22.413393452424117
90-91	22.3129136570217	27.856635357522386	27.513350174412082	22.317100811043833
92-93	22.322695099583118	27.84575956312113	27.46280922254397	22.368736114751783
94-95	22.185996947197278	27.884627186309245	27.524742312374872	22.404633554118604
96-97	22.22375367228964	27.900862275999323	27.580459718106447	22.294924333604584
98-99	22.427567775603613	28.505989140034625	27.61935402872687	21.447089055634887
100-101	26.53575008111682	35.507609291186476	28.21606382905281	9.74057679864389
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3480.0
1	3062.0
2	3927.0
3	5592.0
4	7629.0
5	12094.5
6	15742.5
7	18233.0
8	19814.5
9	19446.0
10	17800.0
11	16085.5
12	17283.5
13	22620.0
14	34984.0
15	51496.5
16	64863.5
17	73223.5
18	76045.5
19	73871.0
20	70269.5
21	68276.5
22	70043.0
23	78401.5
24	94359.5
25	117965.0
26	148665.0
27	183492.0
28	222219.5
29	263440.0
30	302275.5
31	339926.0
32	378324.5
33	415606.5
34	451038.5
35	481907.0
36	514029.5
37	544454.0
38	567439.5
39	588260.0
40	611258.0
41	632441.0
42	646315.0
43	661906.0
44	677157.5
45	686479.5
46	690926.5
47	692649.0
48	692648.5
49	684829.0
50	671783.0
51	653894.0
52	628486.5
53	600222.5
54	566985.0
55	528933.5
56	489165.0
57	444207.5
58	395298.5
59	346372.0
60	295632.5
61	244808.5
62	198657.0
63	157646.0
64	120667.5
65	91203.0
66	69415.5
67	53306.5
68	40761.0
69	29690.0
70	21317.0
71	15447.5
72	11066.0
73	7884.5
74	5670.5
75	4018.0
76	2838.0
77	1962.5
78	1342.5
79	894.0
80	610.0
81	422.5
82	299.0
83	209.5
84	151.0
85	116.0
86	95.5
87	76.5
88	50.0
89	29.0
90	17.0
91	12.5
92	9.5
93	5.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004543386559982593
3	1.2362956625802973E-5
4	5.006997433450205E-4
5	0.0
6	0.0
7	0.0019471656685639683
8	0.0010075809650029425
9	4.327034819031041E-5
10-11	1.2362956625802974E-4
12-13	1.0817587047577601E-4
14-15	4.636108734676115E-5
16-17	3.61616481304737E-4
18-19	0.0
20-21	7.727163142049986E-5
22-23	0.0
24-25	0.0
26-27	6.093558894818956E-4
28-29	1.8572827989796586E-4
30-31	3.72077574825653E-5
32-33	0.0017476268812876862
34-35	5.056386354860752E-4
36-37	0.0013556829806899591
38-39	0.003853222955262563
40-41	1.2449597087943476E-4
42-43	8.634212551760976E-4
44-45	0.0017519609506791406
46-47	1.1208623572124376E-4
48-49	2.235643050690437E-4
50-51	1.8566076429717848E-4
52-53	0.0
54-55	1.9664307501747016E-4
56-57	0.0017105537149648491
58-59	0.0031561327936687907
60-61	0.0011451312918075805
62-63	0.0010467529488099054
64-65	3.6650444080603267E-6
66-67	9.277960190722204E-5
68-69	7.521010318374896E-6
70-71	3.0496169242760672E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	2.0685324664031024E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	419.0
20-21	1369.0
22-23	2788.0
24-25	6215.0
26-27	9761.0
28-29	20757.0
30-31	44485.0
32-33	55377.0
34-35	75803.0
36-37	119602.0
38-39	143237.0
40-41	140573.0
42-43	158051.0
44-45	184574.0
46-47	185181.0
48-49	176033.0
50-51	162038.0
52-53	156310.0
54-55	168447.0
56-57	175565.0
58-59	174902.0
60-61	170856.0
62-63	161741.0
64-65	167061.0
66-67	175966.0
68-69	178913.0
70-71	177133.0
72-73	167275.0
74-75	167979.0
76-77	166543.0
78-79	176520.0
80-81	177291.0
82-83	168682.0
84-85	165842.0
86-87	172391.0
88-89	182208.0
90-91	192393.0
92-93	197557.0
94-95	212434.0
96-97	311626.0
98-99	1286629.0
100-101	9238833.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.40250694948604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.27682430124267	75.35187783513678
2	7.342030515749036	12.393715632576933
3	1.9003647548815321	4.8118664829134055
4	0.6928544620970081	2.3391461420850055
5	0.31663054036009336	1.336220569158115
6	0.17144276063778413	0.8682119277701789
7	0.0936683019710621	0.5534087655641389
8	0.05594219349318023	0.377732910006211
9	0.03605390458624695	0.27387359391571336
>10	0.1111350397089794	1.4375305669067746
>50	0.002517890109894522	0.1437087677430808
>100	4.737900154780194E-4	0.06417930202648357
>500	4.924976883536858E-5	0.03096859242080529
>1k	1.2295378191863765E-5	0.017558911776387964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.181478312901487E-6
2	0.0	6.181478312901487E-6	0.0	0.0	6.181478312901487E-6
3	0.0	6.181478312901487E-6	0.0	0.0	6.181478312901487E-6
4	0.0	3.708886987740892E-5	0.0	0.0	1.2362956625802974E-5
5	0.0	4.327034819031041E-5	0.0	0.0	1.2362956625802974E-5
6	0.0	4.94518265032119E-5	0.0	0.0	1.2362956625802974E-5
7	0.0	4.94518265032119E-5	0.0	0.0	1.854443493870446E-5
8	0.0	4.94518265032119E-5	0.0	0.0	1.854443493870446E-5
9	0.0	9.89036530064238E-5	0.0	6.181478312901487E-6	1.854443493870446E-5
10-11	0.0	9.89036530064238E-5	0.0	6.181478312901487E-6	1.854443493870446E-5
12-13	0.0	1.1126660963222677E-4	0.0	6.181478312901487E-6	2.1635174095155205E-5
14-15	6.181478312901487E-6	1.8235361023059387E-4	0.0	9.27221746935223E-6	4.017960903385966E-5
16-17	1.854443493870446E-5	1.8853508854349535E-4	0.0	1.854443493870446E-5	4.327034819031041E-5
18-19	2.472591325160595E-5	2.132610017951013E-4	0.0	1.854443493870446E-5	5.872404397256413E-5
20-21	2.472591325160595E-5	2.348961758902565E-4	0.0	2.472591325160595E-5	9.27221746935223E-5
22-23	3.0907391564507436E-5	2.5034987167251024E-4	0.0	5.254256565966264E-5	9.89036530064238E-5
24-25	4.636108734676116E-5	2.9980169817572214E-4	0.0	6.799626144191637E-5	1.1126660963222677E-4
26-27	4.94518265032119E-5	3.3070908974022956E-4	0.0	1.5453695782253717E-4	1.1744808794512825E-4
28-29	6.181478312901487E-5	3.3689056805313107E-4	0.0	4.914275258756682E-4	1.2053882710157899E-4
30-31	7.108700059836709E-5	4.2343126443375185E-4	0.0	0.0016782713619527539	1.2672030541448047E-4
32-33	8.035921806771933E-5	4.4815717768535777E-4	0.0	0.00403341459916822	1.359925228838327E-4
34-35	9.27221746935223E-5	5.192441782837249E-4	0.0	0.007022159363456089	1.421740011967342E-4
36-37	1.0508513131932527E-4	5.996033963514443E-4	0.0	0.011389373791520989	1.483554795096357E-4
38-39	1.1126660963222677E-4	6.428737445417546E-4	0.0	0.018553707156173813	1.5144621866608644E-4
40-41	1.483554795096357E-4	6.954163102014173E-4	0.0	0.026048749610566864	1.5453695782253717E-4
42-43	1.483554795096357E-4	7.263237017659247E-4	0.0	0.033086362669805205	1.5453695782253717E-4
44-45	1.6689991444834016E-4	8.159551373029963E-4	0.0	0.04085029943080948	1.6071843613543865E-4
46-47	1.9162582769994608E-4	8.839513987449126E-4	0.0	0.049223111805634545	1.6071843613543865E-4
48-49	2.0398878432574907E-4	8.839513987449126E-4	0.0	0.057963722140077244	1.6689991444834016E-4
50-51	2.2253321926445354E-4	9.76673573438435E-4	0.0	0.06620054199201847	1.6689991444834016E-4
52-53	2.348961758902565E-4	0.0010106717041593932	0.0	0.07416537679819205	1.7926287107414312E-4
54-55	2.4107765420315798E-4	0.001023034660785196	0.0	0.08221366156158977	1.916258276999461E-4
56-57	2.472591325160595E-4	0.0010323068782545483	0.0	0.09045975363100037	1.9471656685639683E-4
58-59	2.5034987167251024E-4	0.0010415790957239005	0.0	0.09850185691608519	2.1017026263865055E-4
60-61	2.565313499854117E-4	0.0010539420523497036	0.0	0.10657177685357809	2.2253321926445354E-4
62-63	2.5962208914186245E-4	0.0010755772264448588	0.0	0.11442534505011942	2.2871469757735502E-4
64-65	2.627128282983132E-4	0.0011899345752335363	0.0	0.12203165411414471	2.2871469757735502E-4
66-67	2.781665240805669E-4	0.0012022975318593393	0.0	0.12894254686796858	2.2871469757735502E-4
68-69	2.843480023934684E-4	0.0012579308366754524	0.0	0.1363077782777907	2.4416839335960873E-4
70-71	2.905294807063699E-4	0.001301201184865763	0.0	0.14300850076897592	2.5962208914186245E-4
72-73	2.905294807063699E-4	0.001313564141491566	0.0	0.14871709599094043	2.781665240805669E-4
74-75	2.905294807063699E-4	0.0013228363589609183	6.181478312901487E-6	0.15370863972860838	2.781665240805669E-4
76-77	2.967109590192714E-4	0.0013413807938996228	6.181478312901487E-6	0.158094398591612	2.781665240805669E-4
78-79	3.028924373321729E-4	0.0013661067071512286	6.181478312901487E-6	0.16183110223176095	2.8125726323701767E-4
80-81	3.0907391564507434E-4	0.0013661067071512286	6.181478312901487E-6	0.1635340995069653	2.874387415499191E-4
82-83	3.1525539395797585E-4	0.00137228818546413	6.181478312901487E-6	0.16410897699006513	3.028924373321729E-4
84-85	3.1525539395797585E-4	0.0013908326204028346	6.181478312901487E-6	0.16464058412497468	3.0598317648862364E-4
86-87	3.1525539395797585E-4	0.001421740011967342	6.181478312901487E-6	0.16489711547496005	3.152553939579758E-4
88-89	3.3070908974022956E-4	0.0014681010993141033	6.181478312901487E-6	0.164965111736402	3.3070908974022956E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16175	0.0	44.21954	1
GTATCAA	28665	0.0	35.103287	1
TCAACGC	33545	0.0	29.058096	4
ATCAACG	33710	0.0	28.964891	3
CAACGCA	34100	0.0	28.560793	5
AACGCAG	35405	0.0	27.613535	6
TATCAAC	36290	0.0	27.214703	2
ACGCAGA	42115	0.0	23.174679	7
GTACATG	48340	0.0	22.92221	1
CGCAGAG	42665	0.0	22.884853	8
TACATGG	47590	0.0	22.666206	2
ACATGGG	48270	0.0	21.84866	3
CATGGGG	34015	0.0	19.822327	4
GCAGAGT	52235	0.0	18.652317	9
GAGTACT	27450	0.0	17.513144	12-13
AGAGTAC	46170	0.0	17.256767	10-11
CAGAGTA	50425	0.0	16.604988	10-11
GAGTACA	33990	0.0	15.806594	1
GTGGTAT	9560	0.0	15.673482	1
ATGGGGG	22280	0.0	15.512221	5
>>END_MODULE