FastQCFastQC Report
Fri 27 May 2016
SRR2140018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18611933
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224900.043.3415451
GTATCAA393950.033.2113341
ATCAACG455150.028.1537653
TCAACGC457250.028.0697254
CAACGCA467650.027.454415
AACGCAG482950.026.6703476
TATCAAC485000.026.626892
ACGCAGA558650.023.0267437
CGCAGAG567300.022.6970988
GTACATG601100.021.6628171
TACATGG589900.021.5972182
ACATGGG598650.020.6722773
GCAGAGT678500.018.8489469
CATGGGG406700.018.7967514
GAGTACT348600.018.1238412-13
AGAGTAC596600.017.24291610-11
CAGAGTA660100.016.8444710-11
GTACTTT405250.015.60152214-15
ATGGGGG255100.015.3649385
GAGTACA426650.015.124371
Produced by FastQC (version 0.11.5)