##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18611933
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.416291311601	33.0	31.0	34.0	31.0	34.0
2	32.59310760467491	34.0	31.0	34.0	31.0	34.0
3	32.68888051552732	34.0	31.0	34.0	31.0	34.0
4	36.180359235120825	37.0	35.0	37.0	35.0	37.0
5	35.98753987562711	37.0	35.0	37.0	35.0	37.0
6	36.054444425519904	37.0	35.0	37.0	35.0	37.0
7	36.03905424546714	37.0	35.0	37.0	35.0	37.0
8	36.040391559544084	37.0	35.0	37.0	35.0	37.0
9	37.9165042126468	39.0	38.0	39.0	35.0	39.0
10-11	37.763854377726375	39.0	37.5	39.0	35.0	39.0
12-13	37.77055274162012	39.0	38.0	39.0	35.0	39.0
14-15	39.11498706233253	40.0	38.5	41.0	36.0	41.0
16-17	39.078556644277626	40.0	38.0	41.0	36.0	41.0
18-19	39.11800687225771	40.0	38.5	41.0	36.0	41.0
20-21	39.12721043894841	40.0	39.0	41.0	36.0	41.0
22-23	39.06518105219175	40.0	39.0	41.0	36.0	41.0
24-25	39.08866566997936	40.0	39.0	41.0	36.0	41.0
26-27	38.97999453528604	40.0	39.0	41.0	35.5	41.0
28-29	38.90058058821439	40.0	38.0	41.0	35.0	41.0
30-31	38.8006461486817	40.0	38.0	41.0	35.0	41.0
32-33	38.72515177628601	40.0	38.0	41.0	35.0	41.0
34-35	38.67601751700411	40.0	38.0	41.0	35.0	41.0
36-37	38.66621117716163	40.0	38.0	41.0	35.0	41.0
38-39	38.58816746084237	40.0	38.0	41.0	35.0	41.0
40-41	38.56240710575319	40.0	38.0	41.0	35.0	41.0
42-43	38.518257425961586	40.0	38.0	41.0	34.0	41.0
44-45	38.42726716432237	40.0	38.0	41.0	34.0	41.0
46-47	38.36378724002132	40.0	38.0	41.0	34.0	41.0
48-49	38.37145506122718	40.0	38.0	41.0	34.0	41.0
50-51	38.31025149544416	40.0	38.0	41.0	34.0	41.0
52-53	38.13720159046434	40.0	37.5	41.0	33.5	41.0
54-55	37.98018048879453	40.0	37.0	41.0	33.0	41.0
56-57	37.86337496153743	40.0	37.0	41.0	33.0	41.0
58-59	37.70891432691805	39.0	36.5	41.0	33.0	41.0
60-61	37.53976697499478	39.0	36.0	41.0	33.0	41.0
62-63	37.38696189713892	39.0	35.5	40.5	32.5	41.0
64-65	37.18461193838631	39.0	35.0	40.0	32.0	41.0
66-67	36.90345791420994	38.0	35.0	40.0	32.0	41.0
68-69	36.66041293016424	37.5	35.0	40.0	32.0	41.0
70-71	36.37319809041683	37.0	35.0	39.5	31.5	41.0
72-73	36.068946424200334	36.5	35.0	39.0	31.0	41.0
74-75	35.63585571871498	36.0	34.5	39.0	31.0	40.5
76-77	33.699058972264105	34.5	32.0	36.5	28.0	39.0
78-79	34.78434715946973	35.0	34.0	37.0	30.0	39.0
80-81	34.755957727523224	35.0	34.0	37.0	31.0	39.0
82-83	34.508839120968986	35.0	34.0	36.5	31.0	39.0
84-85	34.24352876943703	35.0	34.0	36.0	30.0	37.0
86-87	34.00528807850038	35.0	34.0	36.0	30.0	37.0
88-89	33.845133540488504	35.0	34.0	35.0	30.0	37.0
90-91	33.76039520531411	35.0	34.0	35.0	30.0	36.0
92-93	33.64043972071061	35.0	34.0	35.0	30.0	36.0
94-95	33.53786268370716	35.0	34.0	35.0	30.0	36.0
96-97	33.52272130377285	35.0	34.0	35.0	30.0	36.0
98-99	33.640454847422134	35.0	34.0	35.0	30.5	35.0
100-101	32.432046467643275	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	8.0
19	24.0
20	73.0
21	366.0
22	1458.0
23	4963.0
24	12608.0
25	27349.0
26	50946.0
27	83349.0
28	125268.0
29	175341.0
30	235390.0
31	312527.0
32	414248.0
33	565901.0
34	833716.0
35	1287423.0
36	2307440.0
37	4345577.0
38	5402216.0
39	2383751.0
40	41991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11120360254897	20.28115510624286	13.48669694867266	24.12094434253551
2	14.715029358918146	21.64338478666426	41.08832714345893	22.55325871095866
3	18.346022237026347	26.58884776652138	30.337196070628032	24.727933925824246
4	12.258989039301337	18.020852401594876	39.409175686407714	30.310982872696073
5	12.819039269053892	38.31764277251589	35.923931168245666	12.939386790184557
6	29.515732165795576	38.40743131879055	19.11795615844717	12.958880356966704
7	26.251007641120665	32.77282795074905	23.689768341002683	17.286396067127612
8	24.344900076451584	36.80083456825917	21.633606330700577	17.220659024588674
9	25.752952784462263	16.528463150827395	21.360832979975633	36.357751084734716
10-11	23.989247959465093	26.94985237677661	29.35137121076618	19.709528452992114
12-13	24.83637424052317	24.51913378572293	29.401279798967405	21.243212174786493
14-15	21.860903107526408	25.878461634719095	27.03275112507838	25.227884132676117
16-17	21.4615432868303	29.004674033919486	27.658890221521293	21.87489245772892
18-19	21.46027766164858	28.36338654346112	29.216959893418913	20.959375901471383
20-21	22.373990025095768	27.550219207821396	28.97659064431743	21.099200122765406
22-23	21.992275517470095	27.50480234366515	28.842053607734652	21.660868531130102
24-25	21.71522362481017	27.606335795756067	29.010275267209785	21.668165312223977
26-27	21.70239818888947	27.78356476346805	28.87020688227378	21.643830165368698
28-29	21.607515404833055	27.98327122392092	28.838470125352	21.570743245894022
30-31	21.9318352268891	27.788043045392374	28.748126558337567	21.531995169380956
32-33	21.687757069445226	27.900485734151324	28.73980231610077	21.671954880302675
34-35	21.76282974625877	27.982671367757295	28.73879769628902	21.515701189694912
36-37	21.75297120353067	27.884772214595767	28.747595674147895	21.61466090772567
38-39	21.603265492394915	28.034697685796832	28.782981473463693	21.579055348344557
40-41	21.84027795220208	27.91242686101954	28.668831769860986	21.578463416917394
42-43	21.773622802717277	27.957708523005813	28.496443728552418	21.772224945724492
44-45	21.856017637490677	28.110824000101715	28.297552346787736	21.735606015619872
46-47	21.883246405222067	28.066141454074028	28.097976342927577	21.952635797776324
48-49	21.855203723649062	28.152346041170613	28.11194616569312	21.880504069487205
50-51	21.81354285463972	28.250030035129654	28.022682013179285	21.913745097051343
52-53	21.973732428157046	28.15761611571292	27.910352418808564	21.95829903732147
54-55	21.955353153300898	28.096574030011766	27.956515398123756	21.99155741856358
56-57	21.964984027933223	28.20518191072144	27.883819082444422	21.94601497890092
58-59	21.933475127626235	28.247062779096442	27.913906212348888	21.905555880928436
60-61	21.976362236074504	28.237514666566803	27.90265831921821	21.883464778140485
62-63	21.955221221556464	28.209730197960592	27.87372725681745	21.9613213236655
64-65	21.895827424124324	28.21622980794024	27.884470214991236	22.003472552944203
66-67	22.00282915928422	28.228536724748103	27.70070811513759	22.067926000830088
68-69	21.983182758927924	28.239772049415336	27.786699947455514	21.99034524420123
70-71	21.922066224120364	28.021526466980625	27.873346806739246	22.183060502159773
72-73	21.98129954429445	28.065702469138454	27.8754271568739	22.0775708296932
74-75	21.885697476349772	28.19610209589471	27.78059689074997	22.137603537005553
76-77	21.93545731272146	28.174210646257407	27.735394249988016	22.15493779103312
78-79	21.987473232224502	28.162985840270593	27.70223196538786	22.147308962117048
80-81	21.931770053254557	28.171455543183395	27.740642416046494	22.156131987515554
82-83	21.985100240952146	28.129327889922727	27.823004137115053	22.062567732010073
84-85	22.145364031334765	28.11714865356045	27.68198163839695	22.05550567670784
86-87	21.9117249564797	28.159650814410924	27.79996122177898	22.1286630073304
88-89	22.006371973953915	28.13003786883339	27.77296906676187	22.090621090450824
90-91	22.056729296282324	28.14418924502524	27.78843984387203	22.010641614820404
92-93	22.060103464080218	28.10060883083422	27.767482208885337	22.071805496200227
94-95	21.90498589677878	28.193551824559666	27.80924525323178	22.09221702542978
96-97	21.916702970921417	28.216831800081856	27.874034103047883	21.992431125948844
98-99	22.13654120082387	28.778596115977784	27.921277877699314	21.163584805499035
100-101	26.08873203552646	35.870361017515826	28.538431618004555	9.502475328953162
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4858.0
1	4217.0
2	5271.5
3	7468.0
4	10016.5
5	15633.0
6	20219.0
7	23282.5
8	25452.5
9	25050.0
10	22851.5
11	20729.0
12	22339.0
13	29187.0
14	44685.0
15	65437.5
16	82602.5
17	93190.0
18	96250.5
19	93063.5
20	87589.5
21	83334.5
22	84365.0
23	94951.0
24	115125.5
25	143761.0
26	180301.5
27	221191.0
28	266686.5
29	315504.5
30	359293.5
31	402035.0
32	447937.0
33	492161.5
34	534820.0
35	574423.0
36	615236.0
37	652873.5
38	680578.5
39	702736.5
40	725709.5
41	747561.0
42	761837.5
43	776570.0
44	793065.5
45	804355.0
46	809847.0
47	813634.5
48	810314.0
49	795184.0
50	775161.0
51	744394.0
52	705351.0
53	666349.5
54	623139.5
55	572575.0
56	520168.0
57	468209.5
58	412705.0
59	357603.0
60	303146.5
61	248900.0
62	198755.0
63	155579.0
64	118664.5
65	89318.5
66	67044.0
67	50961.0
68	38869.5
69	28219.0
70	20333.0
71	14809.5
72	10777.0
73	7704.0
74	5420.5
75	3779.0
76	2621.5
77	1736.5
78	1139.5
79	779.5
80	569.0
81	401.0
82	276.0
83	201.0
84	148.5
85	103.0
86	76.5
87	58.5
88	42.0
89	31.5
90	24.5
91	19.0
92	16.0
93	9.5
94	3.5
95	1.5
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0047979970699443195
3	1.0745794109617739E-5
4	3.8684858794623854E-4
5	0.0
6	5.372897054808869E-6
7	0.002127667233704312
8	8.274261464405659E-4
9	4.835607349327982E-5
10-11	1.2894952931541285E-4
12-13	1.3700887489762618E-4
14-15	4.0296727911066514E-5
16-17	3.519247570899809E-4
18-19	0.0
20-21	1.074626005970837E-4
22-23	0.0
24-25	0.0
26-27	6.748599685730349E-4
28-29	1.560648243770242E-4
30-31	2.9648919596065276E-5
32-33	0.0019167728574149437
34-35	4.7217645939838743E-4
36-37	0.0013368934350783813
38-39	0.003919348229717043
40-41	1.5819584356795683E-4
42-43	9.244259384954211E-4
44-45	0.00196236339911923
46-47	6.594037831543205E-5
48-49	2.3506804262149277E-4
50-51	2.1432646777150885E-4
52-53	0.0
54-55	2.0101772574305601E-4
56-57	0.0016118423239378712
58-59	0.0032078756912526573
60-61	0.0012099197745431137
62-63	0.0010922148155508853
64-65	0.0
66-67	1.2895554199659673E-4
68-69	1.3064266917605107E-5
70-71	4.634129209717451E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.9482095408124624E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	525.0
20-21	1646.0
22-23	3181.0
24-25	7783.0
26-27	11871.0
28-29	24093.0
30-31	53469.0
32-33	64652.0
34-35	87386.0
36-37	136999.0
38-39	164234.0
40-41	164116.0
42-43	182567.0
44-45	214442.0
46-47	214464.0
48-49	203042.0
50-51	186747.0
52-53	178411.0
54-55	191020.0
56-57	199540.0
58-59	197192.0
60-61	193134.0
62-63	184286.0
64-65	188470.0
66-67	199264.0
68-69	203508.0
70-71	200183.0
72-73	190838.0
74-75	191935.0
76-77	189600.0
78-79	201136.0
80-81	203086.0
82-83	193379.0
84-85	190741.0
86-87	197372.0
88-89	206929.0
90-91	219036.0
92-93	226248.0
94-95	242143.0
96-97	355288.0
98-99	1476797.0
100-101	1.067118E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.60921765868363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1265300901412	74.5179945346983
2	7.276051941911198	12.16690021014282
3	1.9214917800499272	4.819632734026974
4	0.7736679348374828	2.587430830374854
5	0.3557387118873385	1.4871517695904117
6	0.18908353459028138	0.9485475839539242
7	0.1118102943033623	0.6543859863023904
8	0.06434747838012457	0.4304033860537019
9	0.04196535365908426	0.31578213493854107
>10	0.13600062096303686	1.707285954330689
>50	0.0025645615807635786	0.13783397952986892
>100	6.369134359474492E-4	0.09199375022018831
>500	3.326646027348857E-5	0.021264209232787785
>1k	7.751780010543557E-5	0.11339293660449477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.372897054808869E-6	1.0745794109617739E-5
2	0.0	1.611869116442661E-5	0.0	5.372897054808869E-6	1.0745794109617739E-5
3	0.0	1.611869116442661E-5	0.0	1.0745794109617739E-5	1.0745794109617739E-5
4	0.0	2.1491588219235478E-5	0.0	2.1491588219235478E-5	1.0745794109617739E-5
5	0.0	2.6864485274044346E-5	0.0	2.6864485274044346E-5	1.0745794109617739E-5
6	0.0	2.6864485274044346E-5	0.0	2.6864485274044346E-5	1.611869116442661E-5
7	0.0	2.6864485274044346E-5	0.0	4.8356073493279824E-5	1.611869116442661E-5
8	1.0745794109617739E-5	2.6864485274044346E-5	0.0	5.910186760289756E-5	1.611869116442661E-5
9	1.0745794109617739E-5	5.910186760289756E-5	0.0	9.671214698655965E-5	1.611869116442661E-5
10-11	2.6864485274044346E-5	5.910186760289756E-5	0.0	1.1014438962358182E-4	1.611869116442661E-5
12-13	2.6864485274044346E-5	5.910186760289756E-5	0.0	1.23576632260604E-4	1.611869116442661E-5
14-15	3.492383085625765E-5	1.0477149256877295E-4	0.0	1.5312756606205278E-4	2.6864485274044346E-5
16-17	4.566962496587539E-5	1.1283083815098625E-4	0.0	1.773056028086927E-4	2.6864485274044346E-5
18-19	4.8356073493279824E-5	1.23576632260604E-4	0.0	2.2566167630197248E-4	4.029672791106652E-5
20-21	4.8356073493279824E-5	1.3432242637022174E-4	0.0	3.250602718159366E-4	4.8356073493279824E-5
22-23	4.8356073493279824E-5	1.5044111753464835E-4	0.0	3.9759438205585633E-4	7.522055876732417E-5
24-25	8.327990434953748E-5	1.6118691164426608E-4	0.0	4.889336319876071E-4	1.0745794109617738E-4
26-27	9.402569845915521E-5	1.6118691164426608E-4	0.0	6.044509186659977E-4	1.1283083815098625E-4
28-29	9.671214698655965E-5	1.6387336017167052E-4	0.0	0.001364715851921453	1.1283083815098625E-4
30-31	1.1820373520579512E-4	2.0685653661014147E-4	0.0	0.0037072989678181194	1.23576632260604E-4
32-33	1.2357663226060398E-4	2.095429851375459E-4	0.0	0.007771895589781029	1.396953234250306E-4
34-35	1.3700887489762618E-4	2.552126101034213E-4	0.0	0.013252250585686076	1.396953234250306E-4
36-37	1.5044111753464835E-4	2.9013644095967896E-4	0.0	0.020986535896083444	1.477546690072439E-4
38-39	1.5044111753464835E-4	2.955093380144878E-4	0.0	0.03288212997543028	1.5044111753464835E-4
40-41	1.5581401458945722E-4	3.1162802917891443E-4	0.0	0.044288790422789506	1.5581401458945722E-4
42-43	1.5581401458945722E-4	3.1968737476112776E-4	0.0	0.05400298829788394	1.5581401458945722E-4
44-45	1.5850046311686165E-4	3.492383085625765E-4	0.0	0.0647622146501387	1.5850046311686165E-4
46-47	1.6387336017167052E-4	3.7610279383662085E-4	0.0	0.07644020639876578	1.7999205133609712E-4
48-49	1.6655980869907495E-4	3.814756908914297E-4	0.0	0.08751106078019946	1.8805139691831043E-4
50-51	1.6655980869907495E-4	4.2714531585730513E-4	0.0	0.0978941843386176	1.9073784544571486E-4
52-53	1.692462572264794E-4	4.2983176438470954E-4	0.0	0.10747405978734181	2.0417008808273703E-4
54-55	1.7193270575388382E-4	4.352046614395184E-4	0.0	0.11739511419904639	2.2028877924716364E-4
56-57	1.773056028086927E-4	4.51323352603945E-4	0.0	0.1269105148831129	2.256616763019725E-4
58-59	1.773056028086927E-4	4.566962496587539E-4	0.0	0.13632651697166542	2.2834812482937694E-4
60-61	1.8805139691831043E-4	4.755013893505849E-4	0.0	0.14517030552388083	2.3103457335678138E-4
62-63	1.8805139691831043E-4	4.916200805150114E-4	0.0	0.15395230575996594	2.3640747041159024E-4
64-65	1.8805139691831043E-4	5.104252202068426E-4	0.0	0.16180210835704167	2.3909391893899468E-4
66-67	1.934242939731193E-4	5.157981172616515E-4	0.0	0.16975936889521365	2.498397130486124E-4
68-69	2.0417008808273703E-4	5.211710143164603E-4	0.0	0.17815989344040728	2.525261615760168E-4
70-71	2.0685653661014147E-4	5.319168084260781E-4	0.0	0.18589417875080466	2.5789905863082574E-4
72-73	2.1222943366495034E-4	5.37289705480887E-4	0.0	0.1915330342098266	2.9013644095967896E-4
74-75	2.1491588219235477E-4	5.480354995905047E-4	0.0	0.19649759108846998	2.9282288948708336E-4
76-77	2.1491588219235477E-4	5.990780216111889E-4	0.0	0.20116395218057148	2.955093380144878E-4
78-79	2.2028877924716364E-4	6.178831613030199E-4	0.0	0.20498408198654058	2.955093380144878E-4
80-81	2.2028877924716364E-4	6.178831613030199E-4	0.0	0.20684310436750444	3.0625513212410556E-4
82-83	2.2028877924716364E-4	6.286289554126377E-4	0.0	0.20747173332291707	3.170009262337233E-4
84-85	2.2028877924716364E-4	6.474340951044687E-4	0.0	0.20799290433723355	3.170009262337233E-4
86-87	2.2028877924716364E-4	6.528069921592776E-4	0.0	0.20823468470469994	3.170009262337233E-4
88-89	2.2028877924716364E-4	6.904172715429396E-4	0.0	0.2083233375061043	3.2237382328853217E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22490	0.0	43.341545	1
GTATCAA	39395	0.0	33.211334	1
ATCAACG	45515	0.0	28.153765	3
TCAACGC	45725	0.0	28.069725	4
CAACGCA	46765	0.0	27.45441	5
AACGCAG	48295	0.0	26.670347	6
TATCAAC	48500	0.0	26.62689	2
ACGCAGA	55865	0.0	23.026743	7
CGCAGAG	56730	0.0	22.697098	8
GTACATG	60110	0.0	21.662817	1
TACATGG	58990	0.0	21.597218	2
ACATGGG	59865	0.0	20.672277	3
GCAGAGT	67850	0.0	18.848946	9
CATGGGG	40670	0.0	18.796751	4
GAGTACT	34860	0.0	18.12384	12-13
AGAGTAC	59660	0.0	17.242916	10-11
CAGAGTA	66010	0.0	16.84447	10-11
GTACTTT	40525	0.0	15.601522	14-15
ATGGGGG	25510	0.0	15.364938	5
GAGTACA	42665	0.0	15.12437	1
>>END_MODULE