FastQCFastQC Report
Fri 27 May 2016
SRR2140017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10959669
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108950.044.8168141
GTATCAA190100.034.6360131
ATCAACG211950.029.9996993
TCAACGC212450.029.8481184
CAACGCA218600.029.0474015
AACGCAG229250.027.753916
TATCAAC232800.027.660292
ACGCAGA267200.023.7323747
CGCAGAG270150.023.4258658
GTACATG285500.022.0167941
TACATGG279950.021.6611672
ACATGGG286450.020.6937223
CATGGGG202650.019.065334
GCAGAGT337400.018.8198579
GAGTACT190500.018.00880412-13
AGAGTAC295900.017.28662110-11
CAGAGTA326350.017.03918510-11
GTGGTAT67050.016.3454571
GTACTTT223950.015.4335114-15
ATGGGGG132250.015.2520495
Produced by FastQC (version 0.11.5)