##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10959669
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71585200246467	34.0	31.0	34.0	31.0	34.0
2	32.890720878522885	34.0	31.0	34.0	31.0	34.0
3	32.98623407331006	34.0	31.0	34.0	31.0	34.0
4	36.382416385020385	37.0	37.0	37.0	35.0	37.0
5	36.230554225679626	37.0	37.0	37.0	35.0	37.0
6	36.28485732552689	37.0	37.0	37.0	35.0	37.0
7	36.281154020253716	37.0	37.0	37.0	35.0	37.0
8	36.26211047067206	37.0	37.0	37.0	35.0	37.0
9	38.144338483215144	39.0	39.0	39.0	37.0	39.0
10-11	38.03855193984417	39.0	38.0	39.0	36.0	39.0
12-13	38.07803178179925	39.0	38.0	39.0	37.0	39.0
14-15	39.50075002265123	41.0	39.0	41.0	37.0	41.0
16-17	39.48430258249588	40.5	39.0	41.0	37.0	41.0
18-19	39.52983634815978	41.0	39.0	41.0	37.0	41.0
20-21	39.54550390057966	41.0	39.0	41.0	37.0	41.0
22-23	39.48058748548507	41.0	39.0	41.0	37.0	41.0
24-25	39.43822835452333	41.0	39.0	41.0	37.0	41.0
26-27	39.40133758457996	40.5	39.0	41.0	37.0	41.0
28-29	39.3059221423585	40.0	39.0	41.0	36.0	41.0
30-31	39.21033669489604	40.0	39.0	41.0	36.0	41.0
32-33	39.10311975819674	40.0	39.0	41.0	35.5	41.0
34-35	39.02870082459475	40.0	39.0	41.0	35.0	41.0
36-37	39.033925720510055	40.0	39.0	41.0	35.0	41.0
38-39	39.0278193818737	40.0	39.0	41.0	35.0	41.0
40-41	38.97953147973073	40.0	38.5	41.0	35.0	41.0
42-43	38.9425593893643	40.0	38.0	41.0	35.0	41.0
44-45	38.907269974825894	40.0	38.0	41.0	35.0	41.0
46-47	38.8329538548778	40.0	38.0	41.0	35.0	41.0
48-49	38.8057981597791	40.0	38.0	41.0	35.0	41.0
50-51	38.71319819759087	40.0	38.0	41.0	35.0	41.0
52-53	38.5418240988692	40.0	38.0	41.0	34.5	41.0
54-55	38.40888775305173	40.0	38.0	41.0	34.0	41.0
56-57	38.24896057696074	40.0	37.0	41.0	34.0	41.0
58-59	38.063004217857085	40.0	37.0	41.0	34.0	41.0
60-61	37.940329591248585	39.5	36.5	41.0	34.0	41.0
62-63	37.77392761728622	39.0	36.0	41.0	33.5	41.0
64-65	37.52664444388644	39.0	36.0	41.0	33.0	41.0
66-67	37.2580794883898	39.0	35.0	40.0	33.0	41.0
68-69	37.008148565242024	38.0	35.0	40.0	33.0	41.0
70-71	36.7138908340906	37.0	35.0	40.0	32.5	41.0
72-73	36.36408518616711	37.0	35.0	39.0	32.0	41.0
74-75	35.9836953940101	36.0	35.0	39.0	32.0	40.5
76-77	34.05283695974935	34.5	32.5	36.5	29.0	39.0
78-79	35.126972318304645	35.0	34.0	37.0	31.0	39.0
80-81	35.05061591406083	35.0	34.0	37.0	31.0	39.0
82-83	34.76537222002998	35.0	34.0	37.0	31.0	39.0
84-85	34.5101782248879	35.0	34.0	36.0	31.0	37.5
86-87	34.298651932197316	35.0	34.0	36.0	31.0	37.0
88-89	34.14046452906898	35.0	34.0	35.5	31.0	37.0
90-91	33.9961398324594	35.0	34.0	35.0	31.0	36.0
92-93	33.911211647065535	35.0	34.0	35.0	31.0	36.0
94-95	33.82944630419749	35.0	34.0	35.0	31.0	36.0
96-97	33.79140740052552	35.0	34.0	35.0	31.0	36.0
98-99	33.824664503443046	35.0	34.0	35.0	31.0	35.0
100-101	32.55080277833936	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	33.0
21	119.0
22	568.0
23	1806.0
24	4993.0
25	11103.0
26	21031.0
27	35068.0
28	53444.0
29	76154.0
30	103813.0
31	137697.0
32	184022.0
33	256407.0
34	390263.0
35	631803.0
36	1233802.0
37	2627948.0
38	3588953.0
39	1568518.0
40	32114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10771328951632	20.171996070319274	13.461455815864513	24.258834824299893
2	14.714798752186088	21.418947161345617	41.19665533023098	22.669598756237313
3	18.29241975454864	26.23317651166052	30.3469783305413	25.127425403249536
4	12.271873688086941	17.90739368537153	39.465985404843195	30.354747221698332
5	12.9124099219812	38.10740217813207	36.09712193784008	12.883065962046652
6	29.788271890328076	38.41828617269372	19.00173262531925	12.791709311658956
7	26.365887509923887	32.5239749485135	23.680441443988865	17.42969609757375
8	24.28758153236362	36.98047511748827	21.55642470691234	17.175518643235772
9	25.59925579765653	16.56562845451638	21.333678851504896	36.50143689632219
10-11	23.983291330507193	26.85712408687194	29.454253721401734	19.705330861219135
12-13	24.75310862527034	24.37673634436937	29.45297638993661	21.417178640423682
14-15	21.812569165621888	25.851656715241955	26.88488737349224	25.45088674564392
16-17	21.59626008861162	28.92984002662163	27.567096743641205	21.906803141125547
18-19	21.597769056711474	28.31115155028861	29.023426711153412	21.067652681846504
20-21	22.345876825555568	27.600639089871787	28.82423979192168	21.229244292650968
22-23	22.046315067513113	27.563849276686952	28.65950921589011	21.730326439909827
24-25	21.831980597823073	27.59848318384448	28.778741208702318	21.790795009630127
26-27	21.81814554794505	27.811891812141788	28.627608192103576	21.74235444780959
28-29	21.74414104721486	27.930344194421597	28.633071864635333	21.69244289372821
30-31	22.023603956918755	27.75301908949895	28.582047712772184	21.641329240810116
32-33	21.778546703921972	27.916977975426565	28.506922397703967	21.797552922947496
34-35	21.93621345461966	27.90883578731016	28.553848077110455	21.601102680959723
36-37	21.924706413791252	27.83106203676573	28.532108876583795	21.71212267285922
38-39	21.746715705201357	27.978209876786853	28.59620035713216	21.678874060879632
40-41	22.013460455966204	27.8298786182274	28.48374859398321	21.672912331823188
42-43	21.88262806246566	27.905369299095145	28.35801887303241	21.853983765406785
44-45	21.96854778335787	28.005295046705502	28.218122313964844	21.808034855971787
46-47	21.984943571824633	27.99491917082716	28.00848779525957	22.011649462088638
48-49	21.931906086516946	28.05322554954886	28.080054406696636	21.93481395723756
50-51	21.92617175178083	28.143265806680855	27.97707459627229	21.95348784526602
52-53	22.08193213626463	28.044762628347552	27.843932449543367	22.029372785844448
54-55	22.015958305705823	27.9797741307643	27.940582018253856	22.06368554527602
56-57	22.052871467821543	28.080239022720765	27.871594216813094	21.995295292644602
58-59	22.004852343480856	28.128513857416067	27.888104130907195	21.978529668195883
60-61	22.060850640773246	28.070191003649487	27.91832546522588	21.950632890351386
62-63	22.017792174008736	28.125044071757934	27.84374143978483	22.013422314448505
64-65	21.966168940033747	28.119101156047442	27.865623263365276	22.049106640553536
66-67	22.065358965571562	28.15500982882016	27.70559566301245	22.07403554259583
68-69	21.986897085353412	28.174929365088076	27.76203695708282	22.07613659247569
70-71	22.012740842489322	27.9245397662266	27.83810268791344	22.224616703370632
72-73	22.00023089975961	27.987617621333925	27.81762146388715	22.194530015019314
74-75	21.903915821244883	28.076716692409743	27.779863460315475	22.239504026029902
76-77	22.03007014568677	28.010225324573835	27.701003132470397	22.258701397269
78-79	22.064008454824116	28.057910761638293	27.631671300830035	22.246409482707556
80-81	21.99426984435359	28.084884805353948	27.66024711444603	22.260598235846434
82-83	22.079701447661133	28.018611144007462	27.73352893112126	22.16815847721015
84-85	22.171111378654672	28.01441311910264	27.682506826119546	22.131968676123137
86-87	22.000914798952138	28.07064853935405	27.73064299862698	22.197793663066832
88-89	22.058788535514594	28.04609670515083	27.709036184016977	22.186078575317595
90-91	22.102666570926168	28.032026997381713	27.753599701884713	22.111706729807405
92-93	22.073794858340452	28.032028630161125	27.778256647675644	22.115919863822782
94-95	21.967727893369958	28.073108616599267	27.839131122012507	22.12003236801827
96-97	21.91851611666862	28.096195679064596	27.891329371174024	22.09395883309276
98-99	22.238950460566738	28.693390881199527	27.880864431770835	21.186794226462904
100-101	22.989454509124226	31.300276531051612	24.985043215528886	20.725225744295276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2036.0
1	1766.5
2	2263.5
3	3245.0
4	4389.5
5	7056.5
6	9353.5
7	10990.0
8	12113.5
9	11974.5
10	10879.5
11	9663.5
12	10429.5
13	13765.0
14	21114.0
15	31006.5
16	39169.5
17	44281.0
18	46094.0
19	45013.5
20	42836.5
21	41397.5
22	42158.5
23	47700.5
24	58367.0
25	73179.5
26	92797.5
27	115944.5
28	142425.0
29	170272.5
30	196825.0
31	224128.0
32	252019.5
33	278286.0
34	303043.5
35	326098.0
36	349725.5
37	372104.0
38	389711.0
39	405384.0
40	422567.0
41	437120.0
42	446861.5
43	456176.0
44	465184.0
45	471932.5
46	474619.0
47	474956.0
48	469671.5
49	457466.5
50	444794.0
51	427475.5
52	404194.5
53	380954.5
54	355050.5
55	324946.0
56	295706.5
57	265214.5
58	232623.5
59	200791.5
60	168121.5
61	136602.0
62	107861.0
63	83239.5
64	62624.5
65	46811.0
66	35684.0
67	27792.5
68	21652.0
69	15791.5
70	11450.5
71	8452.0
72	6197.5
73	4502.0
74	3235.5
75	2353.0
76	1669.0
77	1087.5
78	691.0
79	473.0
80	341.0
81	243.5
82	179.0
83	126.5
84	86.0
85	57.5
86	42.5
87	34.5
88	26.5
89	19.0
90	14.0
91	11.5
92	9.0
93	5.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.007359893807012E-4
3	0.004671673934678136
4	5.474617892200941E-5
5	2.7373089461004706E-5
6	0.0
7	0.0
8	5.83959241834767E-4
9	0.002591319135641779
10-11	8.12068320676473E-4
12-13	3.786610708772318E-4
14-15	1.4142762888185765E-4
16-17	0.0014416493782795812
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.728297215128676E-4
32-33	7.516268367078781E-4
34-35	3.217224172549476E-5
36-37	8.630097145357695E-4
38-39	6.500868214167389E-4
40-41	2.80490366348E-5
42-43	1.6949259472729254E-4
44-45	0.0010253885249339833
46-47	0.0
48-49	4.644830158882222E-4
50-51	2.441115890707531E-5
52-53	2.4613345267195832E-5
54-55	8.18770019857406E-4
56-57	0.0010812310355955773
58-59	1.3133537873031623E-4
60-61	1.7846106497099423E-4
62-63	1.0294132179951312E-5
64-65	5.194644633061992E-6
66-67	0.0
68-69	2.120332359977094E-5
70-71	4.3936333252586927E-4
72-73	0.0
74-75	5.471041041178502E-6
76-77	0.0
78-79	3.1294196066632493E-4
80-81	0.0
82-83	1.143668232367538E-5
84-85	2.3130561960472416E-5
86-87	3.333548551906509E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.8515757156216703E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	212.0
20-21	698.0
22-23	1432.0
24-25	3173.0
26-27	4908.0
28-29	10140.0
30-31	22939.0
32-33	28603.0
34-35	39089.0
36-37	61803.0
38-39	73049.0
40-41	74082.0
42-43	83077.0
44-45	98762.0
46-47	99715.0
48-49	94758.0
50-51	86544.0
52-53	79460.0
54-55	86184.0
56-57	90072.0
58-59	91044.0
60-61	93803.0
62-63	88327.0
64-65	92305.0
66-67	98215.0
68-69	100354.0
70-71	99881.0
72-73	94554.0
74-75	95575.0
76-77	94577.0
78-79	100949.0
80-81	102988.0
82-83	97750.0
84-85	96519.0
86-87	100687.0
88-89	108189.0
90-91	117262.0
92-93	116017.0
94-95	129359.0
96-97	202083.0
98-99	930315.0
100-101	6870216.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.91236952216535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10144513210753	75.65814834016456
2	7.734364420285051	13.134864193486651
3	1.8034256071984884	4.593994246925205
4	0.6541567228849543	2.2218398951606386
5	0.31038312063206464	1.3177683116276346
6	0.14742814350709843	0.751108379966494
7	0.08204431297870352	0.48766039145879014
8	0.048451412474476055	0.32912993919228506
9	0.0316665739270956	0.24199954441186572
>10	0.08456910589219085	1.073426022808483
>50	0.0016657892092928053	0.09417280007778055
>100	3.3339837523322887E-4	0.05559098365806351
>500	4.969883363420972E-5	0.024490221179690963
>1k	1.656169415628568E-5	0.01580672988181457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.649745261467294E-5	0.0	0.0	0.0
5	0.0	4.562181576834118E-5	0.0	0.0	0.0
6	1.9161162622703296E-4	5.474617892200942E-5	0.0	0.0	0.0
7	1.9161162622703296E-4	7.299490522934588E-5	0.0	9.124363153668235E-6	0.0
8	1.9161162622703296E-4	8.211926838301413E-5	0.0	1.824872630733647E-5	9.124363153668235E-6
9	2.0073598938070118E-4	1.5511417361236E-4	0.0	1.824872630733647E-5	9.124363153668235E-6
10-11	2.0986035253436943E-4	1.5511417361236E-4	0.0	6.387054207567765E-5	9.124363153668235E-6
12-13	2.2354689726487178E-4	1.5511417361236E-4	0.0	6.843272365251177E-5	9.124363153668235E-6
14-15	2.463578051490424E-4	2.0529817095753532E-4	0.0	8.211926838301413E-5	3.649745261467294E-5
16-17	3.1935271037838824E-4	2.1442253411120354E-4	0.0	9.124363153668236E-5	4.562181576834118E-5
18-19	3.1935271037838824E-4	2.463578051490424E-4	0.0	1.0036799469035059E-4	5.474617892200942E-5
20-21	3.1935271037838824E-4	2.646065314563788E-4	0.0	1.4598981045869176E-4	5.474617892200942E-5
22-23	3.1935271037838824E-4	2.6916871303321295E-4	0.0	1.7336289991969647E-4	5.9308360498843534E-5
24-25	3.1935271037838824E-4	3.1935271037838824E-4	0.0	2.2354689726487178E-4	6.843272365251177E-5
26-27	3.1935271037838824E-4	3.376014366857247E-4	0.0	3.376014366857247E-4	7.299490522934588E-5
28-29	3.1935271037838824E-4	3.421636182625588E-4	0.0	9.352472232509941E-4	7.299490522934588E-5
30-31	3.1935271037838824E-4	3.7409888930039765E-4	0.0	0.0025365729567197693	7.299490522934588E-5
32-33	3.1935271037838824E-4	3.7409888930039765E-4	0.0	0.0054061851685484295	7.299490522934588E-5
34-35	3.3303925510889064E-4	4.379694313760753E-4	0.0	0.009211044603628083	1.0036799469035059E-4
36-37	3.376014366857247E-4	5.018399734517529E-4	0.0	0.015146442835089272	1.0949235784401884E-4
38-39	3.376014366857247E-4	5.109643366054212E-4	0.0	0.027546452360924404	1.0949235784401884E-4
40-41	3.376014366857247E-4	5.748348786810988E-4	0.0	0.055567371605839556	1.0949235784401884E-4
42-43	3.376014366857247E-4	6.113323312957718E-4	0.0	0.06619269249828622	1.1405453942085295E-4
44-45	3.512879814162271E-4	7.208246891397907E-4	0.0	0.08013471939709128	1.1861672099768707E-4
46-47	3.6497452614672944E-4	7.938195943691365E-4	0.0	0.09606585746339602	1.323032657281894E-4
48-49	3.695367077235635E-4	8.029439575228047E-4	0.0	0.11039566979623198	1.4598981045869176E-4
50-51	4.0147197876140236E-4	8.622523180216483E-4	0.0	0.12531856573405636	1.4598981045869176E-4
52-53	4.1515852349190477E-4	8.668144995984824E-4	0.0	0.14556552757204622	1.5967635518919412E-4
54-55	4.1972070506873885E-4	8.713766811753166E-4	0.0	0.1866707835793216	1.7336289991969647E-4
56-57	4.1972070506873885E-4	8.850632259058189E-4	0.0	0.23115205395345426	1.7336289991969647E-4
58-59	4.1972070506873885E-4	9.124363153668236E-4	0.0	0.27750838095566577	1.7336289991969647E-4
60-61	4.5165597610657764E-4	9.352472232509942E-4	0.0	0.3082073007861825	1.7336289991969647E-4
62-63	5.018399734517529E-4	9.626203127119988E-4	0.0	0.32274697347155284	1.7336289991969647E-4
64-65	5.064021550285871E-4	0.00108579921528652	0.0	0.3360366084048706	1.7336289991969647E-4
66-67	5.292130629127576E-4	0.00108579921528652	0.0	0.3425605280597434	1.7336289991969647E-4
68-69	5.383374260664259E-4	0.0011086101231706906	0.0	0.34877878154896835	1.779250814965306E-4
70-71	5.565861523737625E-4	0.0011314210310548612	0.0	0.3544450110673963	1.8248726307336472E-4
72-73	5.83959241834767E-4	0.0011359832126316954	0.0	0.35911668500207444	1.9161162622703296E-4
74-75	5.83959241834767E-4	0.001167918483669534	0.0	0.3630675342476128	1.9161162622703296E-4
76-77	5.930836049884353E-4	0.001199853754707373	0.0	0.3664846082486615	1.9161162622703296E-4
78-79	6.022079681421036E-4	0.0012044159362842072	0.0	0.3696370757182539	1.9161162622703296E-4
80-81	6.022079681421036E-4	0.0012044159362842072	0.0	0.3709920436465737	1.9617380780386707E-4
82-83	6.022079681421036E-4	0.0012044159362842072	0.0	0.37161250034102306	2.0073598938070118E-4
84-85	6.067701497189377E-4	0.0012272268441683777	0.0	0.3722283948538957	2.0073598938070118E-4
86-87	6.113323312957718E-4	0.0012500377520525483	0.0	0.3725021257485057	2.0073598938070118E-4
88-89	6.2045669444944E-4	0.0012865352046672212	0.0	0.3726298668326571	2.0073598938070118E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10895	0.0	44.816814	1
GTATCAA	19010	0.0	34.636013	1
ATCAACG	21195	0.0	29.999699	3
TCAACGC	21245	0.0	29.848118	4
CAACGCA	21860	0.0	29.047401	5
AACGCAG	22925	0.0	27.75391	6
TATCAAC	23280	0.0	27.66029	2
ACGCAGA	26720	0.0	23.732374	7
CGCAGAG	27015	0.0	23.425865	8
GTACATG	28550	0.0	22.016794	1
TACATGG	27995	0.0	21.661167	2
ACATGGG	28645	0.0	20.693722	3
CATGGGG	20265	0.0	19.06533	4
GCAGAGT	33740	0.0	18.819857	9
GAGTACT	19050	0.0	18.008804	12-13
AGAGTAC	29590	0.0	17.286621	10-11
CAGAGTA	32635	0.0	17.039185	10-11
GTGGTAT	6705	0.0	16.345457	1
GTACTTT	22395	0.0	15.43351	14-15
ATGGGGG	13225	0.0	15.252049	5
>>END_MODULE