FastQCFastQC Report
Fri 27 May 2016
SRR2140016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6474330
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71100.044.2310451
GTATCAA124800.033.744371
ATCAACG144250.028.2643173
TCAACGC145050.028.1658924
CAACGCA147250.027.688265
AACGCAG154250.026.404936
TATCAAC156000.026.295712
GTACATG205350.023.5135441
TACATGG203300.023.3788952
ACGCAGA180900.022.5381937
CGCAGAG184200.022.1116188
ACATGGG208700.021.9903833
CATGGGG139300.019.6528194
GTGGTAT39950.018.182111
GCAGAGT224950.018.106079
AGAGTAC202600.017.07711210-11
TGGTATC37450.017.0414582
GAGTACT120500.016.93470412-13
CAGAGTA219700.016.34040310-11
GAGTACA144200.015.839761
Produced by FastQC (version 0.11.5)