FastQCFastQC Report
Fri 27 May 2016
SRR2140015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10093901
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103800.045.9892161
GTATCAA174150.033.8162881
TCAACGC200550.028.3201794
ATCAACG203500.027.9106083
CAACGCA205700.027.5489545
AACGCAG213250.026.6741966
TATCAAC224100.025.8784432
ACGCAGA251950.022.5430627
CGCAGAG256450.022.0808358
GTACATG260650.021.6429461
TACATGG258450.021.2980332
ACATGGG262500.020.3837363
CATGGGG195350.018.923284
GCAGAGT316950.017.9469979
GAGTACT184200.017.9226212-13
AGAGTAC280600.017.40826610-11
CAGAGTA309000.016.88014810-11
GTGGTAT57500.016.8716741
GTACTTT209700.015.75376814-15
GAGTACA193200.015.4842821
Produced by FastQC (version 0.11.5)